Restriction map for
pF1KB5606
(Linear) (Six-base) MAPSORT of: KIBB5606.seq Check: 1400 from: 1 to: 1836
KIBB5606.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 660 1230 1510 1836
Size: 660 570 280 326
Fragments arranged by size:
660 570 326 280
AccI GT'mk_AC
Cuts at: 0 109 532 1836
Size: 109 423 1304
Fragments arranged by size:
1304 423 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1751 1836
Size: 826 925 85
Fragments arranged by size:
925 826 85
AleI CACnn'nnGTG
Cuts at: 0 1504 1836
Size: 1504 332
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1045 1075 1836
Size: 769 276 30 761
Fragments arranged by size:
769 761 276 30
ApaI G_GGCC'C
Cuts at: 0 743 1836
Size: 743 1093
ApoI r'AATT_y
Cuts at: 0 725 1591 1836
Size: 725 866 245
Fragments arranged by size:
866 725 245
AseI AT'TA_AT
Cuts at: 0 1409 1836
Size: 1409 427
AvaI C'yCGr_G
Cuts at: 0 1638 1836
Size: 1638 198
BamHI G'GATC_C
Cuts at: 0 1532 1836
Size: 1532 304
BanII G_rGCy'C
Cuts at: 0 44 433 743 1400 1562 1836
Size: 44 389 310 657 162 274
Fragments arranged by size:
657 389 310 274 162 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1063 1299 1836
Size: 1063 236 537
Fragments arranged by size:
1063 537 236
BbvCI CC'TCA_GC
Cuts at: 0 608 1836
Size: 608 1228
BciVI GTATCCnnnnn_n'
Cuts at: 0 1046 1836
Size: 1046 790
Bme1580I G_kGCm'C
Cuts at: 0 240 743 1769 1836
Size: 240 503 1026 67
Fragments arranged by size:
1026 503 240 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 463 1141 1836
Size: 463 678 695
Fragments arranged by size:
695 678 463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 357 381 417 1836
Size: 357 24 36 1419
Fragments arranged by size:
1419 357 36 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 327 440 1025 1438 1836
Size: 203 124 113 585 413 398
Fragments arranged by size:
585 413 398 203 124 113
Bpu10I CC'TnA_GC
Cuts at: 0 608 735 1836
Size: 608 127 1101
Fragments arranged by size:
1101 608 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 412 1198 1836
Size: 349 63 786 638
Fragments arranged by size:
786 638 349 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 1726 1836
Size: 64 1662 110
Fragments arranged by size:
1662 110 64
BsaAI yAC'GTr
Cuts at: 0 1314 1836
Size: 1314 522
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 435 465 841 871 1250 1280 1836
Size: 435 30 376 30 379 30 556
Fragments arranged by size:
556 435 379 376 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 257 346 443 989 1663 1836
Size: 257 89 97 546 674 173
Fragments arranged by size:
674 546 257 173 97 89
BseYI C'CCAG_C
Cuts at: 0 94 602 618 683 688 1836
Size: 94 508 16 65 5 1148
Fragments arranged by size:
1148 508 94 65 16 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 417 536 688 1836
Size: 44 373 119 152 1148
Fragments arranged by size:
1148 373 152 119 44
BsiHKAI G_wGCw'C
Cuts at: 0 433 1184 1251 1828 1836
Size: 433 751 67 577 8
Fragments arranged by size:
751 577 433 67 8
Bsp1286I G_dGCh'C
Cuts at: 0 44 240 433 743 1184 1251 1400 1562
Size: 44 196 193 310 441 67 149 162
Cuts at: 1562 1769 1828 1836
Size: 207 59 8
Fragments arranged by size:
441 310 207 196 193 162 149 67
59 44 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 660 1230 1336 1510 1792 1836
Size: 660 570 106 174 282 44
Fragments arranged by size:
660 570 282 174 106 44
BsrBI CCG'CTC
Cuts at: 0 1455 1836
Size: 1455 381
BsrDI GCAATG_nn'
Cuts at: 0 1099 1434 1836
Size: 1099 335 402
Fragments arranged by size:
1099 402 335
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1337 1784 1836
Size: 1337 447 52
Fragments arranged by size:
1337 447 52
BstEII G'GTnAC_C
Cuts at: 0 961 1836
Size: 961 875
BstXI CCAn_nnnn'nTGG
Cuts at: 0 85 381 576 974 1836
Size: 85 296 195 398 862
Fragments arranged by size:
862 398 296 195 85
BstYI r'GATC_y
Cuts at: 0 1071 1532 1836
Size: 1071 461 304
Fragments arranged by size:
1071 461 304
BtgI C'CryG_G
Cuts at: 0 632 1295 1600 1836
Size: 632 663 305 236
Fragments arranged by size:
663 632 305 236
BtsI GCAGTG_nn'
Cuts at: 0 787 1289 1429 1566 1692 1836
Size: 787 502 140 137 126 144
Fragments arranged by size:
787 502 144 140 137 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1300 1335 1836
Size: 1300 35 501
Fragments arranged by size:
1300 501 35
EaeI y'GGCC_r
Cuts at: 0 48 1116 1239 1836
Size: 48 1068 123 597
Fragments arranged by size:
1068 597 123 48
EarI CTCTTCn'nnn_
Cuts at: 0 281 1836
Size: 281 1555
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1150 1836
Size: 1150 686
EcoICRI GAG'CTC
Cuts at: 0 431 1836
Size: 431 1405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 327 440 826 1025 1438 1751 1836
Size: 203 124 113 386 199 413 313 85
Fragments arranged by size:
413 386 313 203 199 124 113 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 716 1007 1836
Size: 716 291 829
Fragments arranged by size:
829 716 291
EcoO109I rG'GnC_Cy
Cuts at: 0 739 740 1135 1836
Size: 739 1 395 701
Fragments arranged by size:
739 701 395 1
HaeII r_GCGC'y
Cuts at: 0 342 1836
Size: 342 1494
HincII GTy'rAC
Cuts at: 0 955 1811 1836
Size: 955 856 25
Fragments arranged by size:
955 856 25
MscI TGG'CCA
Cuts at: 0 1118 1836
Size: 1118 718
MslI CAynn'nnrTG
Cuts at: 0 103 574 1294 1504 1804 1836
Size: 103 471 720 210 300 32
Fragments arranged by size:
720 471 300 210 103 32
MspA1I CmG'CkG
Cuts at: 0 1244 1602 1836
Size: 1244 358 234
Fragments arranged by size:
1244 358 234
NcoI C'CATG_G
Cuts at: 0 1295 1836
Size: 1295 541
PasI CC'CwG_GG
Cuts at: 0 744 1836
Size: 744 1092
PflMI CCAn_nnn'nTGG
Cuts at: 0 919 1045 1114 1836
Size: 919 126 69 722
Fragments arranged by size:
919 722 126 69
PfoI T'CCnGG_A
Cuts at: 0 312 1002 1836
Size: 312 690 834
Fragments arranged by size:
834 690 312
PmlI CAC'GTG
Cuts at: 0 1314 1836
Size: 1314 522
PshAI GACnn'nnGTC
Cuts at: 0 469 1836
Size: 469 1367
PspOMI G'GGCC_C
Cuts at: 0 739 1836
Size: 739 1097
SacI G_AGCT'C
Cuts at: 0 433 1836
Size: 433 1403
SacII CC_GC'GG
Cuts at: 0 1603 1836
Size: 1603 233
SmaI CCC'GGG
Cuts at: 0 1640 1836
Size: 1640 196
SmlI C'TyrA_G
Cuts at: 0 328 391 1177 1836
Size: 328 63 786 659
Fragments arranged by size:
786 659 328 63
StuI AGG'CCT
Cuts at: 0 654 1480 1836
Size: 654 826 356
Fragments arranged by size:
826 654 356
StyI C'CwwG_G
Cuts at: 0 1295 1743 1757 1817 1836
Size: 1295 448 14 60 19
Fragments arranged by size:
1295 448 60 19 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 23 39 91 138 980 1836
Size: 23 16 52 47 842 856
Fragments arranged by size:
856 842 52 47 23 16
TatI w'GTAC_w
Cuts at: 0 161 300 1836
Size: 161 139 1536
Fragments arranged by size:
1536 161 139
XbaI T'CTAG_A
Cuts at: 0 222 1836
Size: 222 1614
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1024 1446 1836
Size: 1024 422 390
Fragments arranged by size:
1024 422 390
XmaI C'CCGG_G
Cuts at: 0 1638 1836
Size: 1638 198
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI AseI
AvaI BamHI BanII BbsI BbvCI BciVI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstAPI
BstEII BstXI BstYI BtgI BtsI CspCI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII HincII MscI
MslI MspA1I NcoI PasI PflMI PfoI PmlI PshAI
PspOMI SacI SacII SmaI SmlI StuI StyI TaqII
TatI XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AsiSI AvrII BaeI BanI
BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PpiI PpuMI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XhoI XmnI ZraI