Restriction map for
pF1KB5607
(Linear) (Six-base) MAPSORT of: KIBB5607.seq Check: 6168 from: 1 to: 1395
KIBB5607.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 409 835 1395
Size: 409 426 560
Fragments arranged by size:
560 426 409
AccI GT'mk_AC
Cuts at: 0 530 608 1395
Size: 530 78 787
Fragments arranged by size:
787 530 78
Acc65I G'GTAC_C
Cuts at: 0 646 1395
Size: 646 749
AflIII A'CryG_T
Cuts at: 0 253 1395
Size: 253 1142
AhdI GACnn_n'nnGTC
Cuts at: 0 1367 1395
Size: 1367 28
AleI CACnn'nnGTG
Cuts at: 0 566 658 1395
Size: 566 92 737
Fragments arranged by size:
737 566 92
AlwNI CAG_nnn'CTG
Cuts at: 0 85 994 1048 1395
Size: 85 909 54 347
Fragments arranged by size:
909 347 85 54
ApaI G_GGCC'C
Cuts at: 0 676 1395
Size: 676 719
AvaI C'yCGr_G
Cuts at: 0 602 908 1309 1395
Size: 602 306 401 86
Fragments arranged by size:
602 401 306 86
BanI G'GyrC_C
Cuts at: 0 646 853 1395
Size: 646 207 542
Fragments arranged by size:
646 542 207
BanII G_rGCy'C
Cuts at: 0 30 266 676 1214 1395
Size: 30 236 410 538 181
Fragments arranged by size:
538 410 236 181 30
BbvCI CC'TCA_GC
Cuts at: 0 834 1395
Size: 834 561
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 697 731 1395
Size: 697 34 664
Fragments arranged by size:
697 664 34
BglII A'GATC_T
Cuts at: 0 1079 1395
Size: 1079 316
Bme1580I G_kGCm'C
Cuts at: 0 253 676 1395
Size: 253 423 719
Fragments arranged by size:
719 423 253
BmrI ACTGGGnnnn_n'
Cuts at: 0 621 1395
Size: 621 774
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 46 702 1395
Size: 46 656 693
Fragments arranged by size:
693 656 46
Bpu10I CC'TnA_GC
Cuts at: 0 677 834 1213 1395
Size: 677 157 379 182
Fragments arranged by size:
677 379 182 157
BsaI GGTCTCn'nnnn_
Cuts at: 0 1003 1277 1395
Size: 1003 274 118
Fragments arranged by size:
1003 274 118
BsaAI yAC'GTr
Cuts at: 0 568 1395
Size: 568 827
BsaWI w'CCGG_w
Cuts at: 0 96 1395
Size: 96 1299
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 492 522 695 725 1013 1043 1395
Size: 492 30 173 30 288 30 352
Fragments arranged by size:
492 352 288 173 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 142 1021 1222 1395
Size: 38 104 879 201 173
Fragments arranged by size:
879 201 173 104 38
BseYI C'CCAG_C
Cuts at: 0 1364 1395
Size: 1364 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 813 1395
Size: 387 426 582
Fragments arranged by size:
582 426 387
BsiHKAI G_wGCw'C
Cuts at: 0 30 266 960 1214 1395
Size: 30 236 694 254 181
Fragments arranged by size:
694 254 236 181 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 777 1395
Size: 777 618
Bsp1286I G_dGCh'C
Cuts at: 0 30 253 266 676 960 1214 1395
Size: 30 223 13 410 284 254 181
Fragments arranged by size:
410 284 254 223 181 30 13
BspEI T'CCGG_A
Cuts at: 0 96 1395
Size: 96 1299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 70 409 835 1395
Size: 70 339 426 560
Fragments arranged by size:
560 426 339 70
BsrDI GCAATG_nn'
Cuts at: 0 316 1395
Size: 316 1079
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 1395
Size: 315 1080
BstXI CCAn_nnnn'nTGG
Cuts at: 0 877 1395
Size: 877 518
BstYI r'GATC_y
Cuts at: 0 537 548 1079 1395
Size: 537 11 531 316
Fragments arranged by size:
537 531 316 11
BstZ17I GTA'TAC
Cuts at: 0 609 1395
Size: 609 786
Bsu36I CC'TnA_GG
Cuts at: 0 1157 1395
Size: 1157 238
BtsI GCAGTG_nn'
Cuts at: 0 65 722 1395
Size: 65 657 673
Fragments arranged by size:
673 657 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 160 1395
Size: 160 1235
EaeI y'GGCC_r
Cuts at: 0 2 1395
Size: 2 1393
EcoICRI GAG'CTC
Cuts at: 0 28 264 1212 1395
Size: 28 236 948 183
Fragments arranged by size:
948 236 183 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 348 1395
Size: 348 1047
EcoO109I rG'GnC_Cy
Cuts at: 0 132 615 672 673 1395
Size: 132 483 57 1 722
Fragments arranged by size:
722 483 132 57 1
HaeII r_GCGC'y
Cuts at: 0 1230 1395
Size: 1230 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 540 572 1019 1051 1353 1385 1395
Size: 540 32 447 32 302 32 10
Fragments arranged by size:
540 447 302 32 32 32 10
HincII GTy'rAC
Cuts at: 0 978 984 1361 1395
Size: 978 6 377 34
Fragments arranged by size:
978 377 34 6
KpnI G_GTAC'C
Cuts at: 0 650 1395
Size: 650 745
MluI A'CGCG_T
Cuts at: 0 253 1395
Size: 253 1142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1082 1395
Size: 1082 313
MscI TGG'CCA
Cuts at: 0 4 1395
Size: 4 1391
MslI CAynn'nnrTG
Cuts at: 0 69 566 658 875 1395
Size: 69 497 92 217 520
Fragments arranged by size:
520 497 217 92 69
MspA1I CmG'CkG
Cuts at: 0 443 554 755 838 991 1188 1395
Size: 443 111 201 83 153 197 207
Fragments arranged by size:
443 207 201 197 153 111 83
NdeI CA'TA_TG
Cuts at: 0 1387 1395
Size: 1387 8
NspI r_CATG'y
Cuts at: 0 212 712 1126 1395
Size: 212 500 414 269
Fragments arranged by size:
500 414 269 212
PfoI T'CCnGG_A
Cuts at: 0 191 1175 1395
Size: 191 984 220
Fragments arranged by size:
984 220 191
PmlI CAC'GTG
Cuts at: 0 568 1395
Size: 568 827
PpuMI rG'GwC_Cy
Cuts at: 0 132 615 1395
Size: 132 483 780
Fragments arranged by size:
780 483 132
PsiI TTA'TAA
Cuts at: 0 1070 1395
Size: 1070 325
PspOMI G'GGCC_C
Cuts at: 0 672 1395
Size: 672 723
PspXI vC'TCGA_Gb
Cuts at: 0 602 1395
Size: 602 793
PstI C_TGCA'G
Cuts at: 0 352 1395
Size: 352 1043
PvuII CAG'CTG
Cuts at: 0 443 755 991 1188 1395
Size: 443 312 236 197 207
Fragments arranged by size:
443 312 236 207 197
SacI G_AGCT'C
Cuts at: 0 30 266 1214 1395
Size: 30 236 948 181
Fragments arranged by size:
948 236 181 30
SanDI GG'GwC_CC
Cuts at: 0 615 1395
Size: 615 780
SfcI C'TryA_G
Cuts at: 0 348 438 1090 1395
Size: 348 90 652 305
Fragments arranged by size:
652 348 305 90
SmaI CCC'GGG
Cuts at: 0 910 1395
Size: 910 485
SmlI C'TyrA_G
Cuts at: 0 602 1395
Size: 602 793
SpeI A'CTAG_T
Cuts at: 0 385 1395
Size: 385 1010
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 749 1295 1395
Size: 749 546 100
Fragments arranged by size:
749 546 100
TatI w'GTAC_w
Cuts at: 0 774 1395
Size: 774 621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 492 524 695 727 1395
Size: 492 32 171 32 668
Fragments arranged by size:
668 492 171 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 326 1107 1137 1395
Size: 326 781 30 258
Fragments arranged by size:
781 326 258 30
XhoI C'TCGA_G
Cuts at: 0 602 1395
Size: 602 793
XmaI C'CCGG_G
Cuts at: 0 908 1395
Size: 908 487
Enzymes that do cut:
AarI AccI Acc65I AflIII AhdI AleI AlwNI ApaI
AvaI BanI BanII BbvCI BcgI BglII Bme1580I BmrI
BplI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BstAPI
BstXI BstYI BstZ17I Bsu36I BtsI DrdI EaeI EcoICRI
EcoNI EcoO109I HaeII Hin4I HincII KpnI MluI MmeI
MscI MslI MspA1I NdeI NspI PfoI PmlI PpuMI
PsiI PspOMI PspXI PstI PvuII SacI SanDI SfcI
SmaI SmlI SpeI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BpmI BpuEI BsaBI BsaHI BsiEI BsiWI BsmI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BtgI BtgZI ClaI CspCI DraI DraIII EagI EarI
EciI Eco57MI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI MfeI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PshAI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI SspI StuI StyI SwaI Tth111I
XbaI XmnI ZraI