Restriction map for
pF1KB5611
(Linear) (Six-base) MAPSORT of: KIBB5611.seq Check: 8007 from: 1 to: 1146
KIBB5611.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 523 1146
Size: 523 623
AccI GT'mk_AC
Cuts at: 0 815 1146
Size: 815 331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 701 1146
Size: 677 24 445
Fragments arranged by size:
677 445 24
AhdI GACnn_n'nnGTC
Cuts at: 0 775 1146
Size: 775 371
AlwNI CAG_nnn'CTG
Cuts at: 0 655 1146
Size: 655 491
ApaI G_GGCC'C
Cuts at: 0 588 1146
Size: 588 558
ApoI r'AATT_y
Cuts at: 0 521 1146
Size: 521 625
BanII G_rGCy'C
Cuts at: 0 588 749 1146
Size: 588 161 397
Fragments arranged by size:
588 397 161
BclI T'GATC_A
Cuts at: 0 690 1146
Size: 690 456
BglII A'GATC_T
Cuts at: 0 676 1146
Size: 676 470
BlpI GC'TnA_GC
Cuts at: 0 76 1146
Size: 76 1070
Bme1580I G_kGCm'C
Cuts at: 0 588 1146
Size: 588 558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 529 1146
Size: 337 192 617
Fragments arranged by size:
617 337 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 811 1146
Size: 423 388 335
Fragments arranged by size:
423 388 335
BsaBI GATnn'nnATC
Cuts at: 0 862 1146
Size: 862 284
BsaHI Gr'CG_yC
Cuts at: 0 977 1146
Size: 977 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 904 934 1146
Size: 904 30 212
Fragments arranged by size:
904 212 30
BseYI C'CCAG_C
Cuts at: 0 1027 1146
Size: 1027 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1146
Size: 383 763
BsiHKAI G_wGCw'C
Cuts at: 0 273 749 1146
Size: 273 476 397
Fragments arranged by size:
476 397 273
BsmI GAATG_Cn'
Cuts at: 0 534 943 1146
Size: 534 409 203
Fragments arranged by size:
534 409 203
Bsp1286I G_dGCh'C
Cuts at: 0 273 588 749 1146
Size: 273 315 161 397
Fragments arranged by size:
397 315 273 161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 523 1146
Size: 523 623
BsrDI GCAATG_nn'
Cuts at: 0 810 1146
Size: 810 336
BstYI r'GATC_y
Cuts at: 0 676 763 1146
Size: 676 87 383
Fragments arranged by size:
676 383 87
BtgI C'CryG_G
Cuts at: 0 615 1146
Size: 615 531
BtsI GCAGTG_nn'
Cuts at: 0 908 1146
Size: 908 238
DraI TTT'AAA
Cuts at: 0 255 1146
Size: 255 891
EarI CTCTTCn'nnn_
Cuts at: 0 737 1146
Size: 737 409
EcoICRI GAG'CTC
Cuts at: 0 747 1146
Size: 747 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 529 677 701 1146
Size: 337 192 148 24 445
Fragments arranged by size:
445 337 192 148 24
EcoO109I rG'GnC_Cy
Cuts at: 0 584 585 625 1146
Size: 584 1 40 521
Fragments arranged by size:
584 521 40 1
EcoRI G'AATT_C
Cuts at: 0 521 1146
Size: 521 625
FspI TGC'GCA
Cuts at: 0 1003 1146
Size: 1003 143
FspAI rTGC'GCAy
Cuts at: 0 1003 1146
Size: 1003 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 761 793 1105 1111 1137 1143 1146
Size: 761 32 312 6 26 6 3
Fragments arranged by size:
761 312 32 26 6 6 3
HincII GTy'rAC
Cuts at: 0 380 816 1146
Size: 380 436 330
Fragments arranged by size:
436 380 330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1146
Size: 902 244
MslI CAynn'nnrTG
Cuts at: 0 853 1146
Size: 853 293
MspA1I CmG'CkG
Cuts at: 0 385 507 1146
Size: 385 122 639
Fragments arranged by size:
639 385 122
NcoI C'CATG_G
Cuts at: 0 615 1146
Size: 615 531
NspI r_CATG'y
Cuts at: 0 87 187 713 1146
Size: 87 100 526 433
Fragments arranged by size:
526 433 100 87
PasI CC'CwG_GG
Cuts at: 0 45 1146
Size: 45 1101
PpuMI rG'GwC_Cy
Cuts at: 0 625 1146
Size: 625 521
PspOMI G'GGCC_C
Cuts at: 0 584 1146
Size: 584 562
PstI C_TGCA'G
Cuts at: 0 267 1146
Size: 267 879
PvuII CAG'CTG
Cuts at: 0 385 1146
Size: 385 761
SacI G_AGCT'C
Cuts at: 0 749 1146
Size: 749 397
SalI G'TCGA_C
Cuts at: 0 814 1146
Size: 814 332
SapI GCTCTTCn'nnn_
Cuts at: 0 737 1146
Size: 737 409
SfcI C'TryA_G
Cuts at: 0 263 386 1146
Size: 263 123 760
Fragments arranged by size:
760 263 123
SmlI C'TyrA_G
Cuts at: 0 438 790 1146
Size: 438 352 356
Fragments arranged by size:
438 356 352
SphI G_CATG'C
Cuts at: 0 87 187 1146
Size: 87 100 959
Fragments arranged by size:
959 100 87
StyI C'CwwG_G
Cuts at: 0 588 615 1146
Size: 588 27 531
Fragments arranged by size:
588 531 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 897 1146
Size: 897 249
Tth111I GACn'n_nGTC
Cuts at: 0 988 1146
Size: 988 158
XmnI GAAnn'nnTTC
Cuts at: 0 521 1146
Size: 521 625
Enzymes that do cut:
AarI AccI AcuI AhdI AlwNI ApaI ApoI BanII
BclI BglII BlpI Bme1580I BpmI BpuEI BsaBI BsaHI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstYI BtgI BtsI DraI EarI EcoICRI Eco57MI EcoO109I
EcoRI FspI FspAI Hin4I HincII MmeI MslI MspA1I
NcoI NspI PasI PpuMI PspOMI PstI PvuII SacI
SalI SapI SfcI SmlI SphI StyI TaqII Tth111I
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoNI EcoRV FalI FseI HaeII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI XmaI ZraI