Restriction map for
pF1KB5616
(Linear) (Six-base) MAPSORT of: KIBB5616.seq Check: 7511 from: 1 to: 2364
KIBB5616.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 384 634 1204 1783 1894 2364
Size: 384 250 570 579 111 470
Fragments arranged by size:
579 570 470 384 250 111
AccI GT'mk_AC
Cuts at: 0 2332 2364
Size: 2332 32
Acc65I G'GTAC_C
Cuts at: 0 481 2364
Size: 481 1883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 232 1187 1251 2364
Size: 217 15 955 64 1113
Fragments arranged by size:
1113 955 217 64 15
AflII C'TTAA_G
Cuts at: 0 2011 2364
Size: 2011 353
AflIII A'CryG_T
Cuts at: 0 569 651 1762 2364
Size: 569 82 1111 602
Fragments arranged by size:
1111 602 569 82
AleI CACnn'nnGTG
Cuts at: 0 1607 2364
Size: 1607 757
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1810 2364
Size: 1810 554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1116 1148 2364
Size: 1116 32 1216
Fragments arranged by size:
1216 1116 32
AlwNI CAG_nnn'CTG
Cuts at: 0 126 477 1009 2364
Size: 126 351 532 1355
Fragments arranged by size:
1355 532 351 126
ApaI G_GGCC'C
Cuts at: 0 83 2149 2364
Size: 83 2066 215
Fragments arranged by size:
2066 215 83
ApoI r'AATT_y
Cuts at: 0 372 2254 2263 2364
Size: 372 1882 9 101
Fragments arranged by size:
1882 372 101 9
AvaI C'yCGr_G
Cuts at: 0 260 1541 2364
Size: 260 1281 823
Fragments arranged by size:
1281 823 260
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 75 108 465 498 2364
Size: 75 33 357 33 1866
Fragments arranged by size:
1866 357 75 33 33
BamHI G'GATC_C
Cuts at: 0 1480 2364
Size: 1480 884
BanI G'GyrC_C
Cuts at: 0 481 2364
Size: 481 1883
BanII G_rGCy'C
Cuts at: 0 83 112 139 181 298 1049 1855 1950
Size: 83 29 27 42 117 751 806 95
Cuts at: 1950 2149 2364
Size: 199 215
Fragments arranged by size:
806 751 215 199 117 95 83 42
29 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 625 2364
Size: 625 1739
BbvCI CC'TCA_GC
Cuts at: 0 283 2364
Size: 283 2081
BciVI GTATCCnnnnn_n'
Cuts at: 0 1852 2364
Size: 1852 512
BfrBI ATG'CAT
Cuts at: 0 802 2364
Size: 802 1562
BglI GCCn_nnn'nGGC
Cuts at: 0 1390 1473 1571 2364
Size: 1390 83 98 793
Fragments arranged by size:
1390 793 98 83
Bme1580I G_kGCm'C
Cuts at: 0 83 228 1882 2149 2362 2364
Size: 83 145 1654 267 213 2
Fragments arranged by size:
1654 267 213 145 83 2
BmgBI CAC'GTC
Cuts at: 0 2330 2364
Size: 2330 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 241 1479 2364
Size: 241 1238 885
Fragments arranged by size:
1238 885 241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1701 2364
Size: 1701 663
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 810 1084 2364
Size: 320 490 274 1280
Fragments arranged by size:
1280 490 320 274
Bpu10I CC'TnA_GC
Cuts at: 0 283 1686 2364
Size: 283 1403 678
Fragments arranged by size:
1403 678 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1210 1228 2364
Size: 305 905 18 1136
Fragments arranged by size:
1136 905 305 18
BsaAI yAC'GTr
Cuts at: 0 654 2351 2364
Size: 654 1697 13
Fragments arranged by size:
1697 654 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 335 365 910 940 1244 1274 1639 1669
Size: 335 30 545 30 304 30 365 30
Cuts at: 1669 2364
Size: 695
Fragments arranged by size:
695 545 365 335 304 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1516 2218 2364
Size: 1516 702 146
Fragments arranged by size:
1516 702 146
BseYI C'CCAG_C
Cuts at: 0 48 1476 1520 2364
Size: 48 1428 44 844
Fragments arranged by size:
1428 844 48 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1722 1821 1884 2364
Size: 1722 99 63 480
Fragments arranged by size:
1722 480 99 63
BsiEI CG_ry'CG
Cuts at: 0 13 25 1809 2247 2364
Size: 13 12 1784 438 117
Fragments arranged by size:
1784 438 117 13 12
BsiHKAI G_wGCw'C
Cuts at: 0 112 157 1049 1498 1687 2364
Size: 112 45 892 449 189 677
Fragments arranged by size:
892 677 449 189 112 45
BsmI GAATG_Cn'
Cuts at: 0 1537 2364
Size: 1537 827
Bsp1286I G_dGCh'C
Cuts at: 0 83 112 139 157 181 228 298 1049
Size: 83 29 27 18 24 47 70 751
Cuts at: 1049 1498 1687 1855 1882 1950 2149 2362 2364
Size: 449 189 168 27 68 199 213 2
Fragments arranged by size:
751 449 213 199 189 168 83 70
68 47 29 27 27 24 18 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 384 530 634 1204 1783 1894 1975 2364
Size: 384 146 104 570 579 111 81 389
Fragments arranged by size:
579 570 389 384 146 111 104 81
BsrBI CCG'CTC
Cuts at: 0 27 2364
Size: 27 2337
BsrDI GCAATG_nn'
Cuts at: 0 1098 1437 1794 2364
Size: 1098 339 357 570
Fragments arranged by size:
1098 570 357 339
BsrFI r'CCGG_y
Cuts at: 0 1738 2364
Size: 1738 626
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1637 2364
Size: 1637 727
BstBI TT'CG_AA
Cuts at: 0 367 2364
Size: 367 1997
BstEII G'GTnAC_C
Cuts at: 0 675 1362 2364
Size: 675 687 1002
Fragments arranged by size:
1002 687 675
BstXI CCAn_nnnn'nTGG
Cuts at: 0 936 2364
Size: 936 1428
BstYI r'GATC_y
Cuts at: 0 1480 2364
Size: 1480 884
BtgI C'CryG_G
Cuts at: 0 13 935 1079 1331 1692 1809 2364
Size: 13 922 144 252 361 117 555
Fragments arranged by size:
922 555 361 252 144 117 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1399 2364
Size: 1399 965
BtsI GCAGTG_nn'
Cuts at: 0 127 898 1010 1614 2039 2364
Size: 127 771 112 604 425 325
Fragments arranged by size:
771 604 425 325 127 112
DrdI GACnn_nn'nnGTC
Cuts at: 0 2068 2364
Size: 2068 296
EaeI y'GGCC_r
Cuts at: 0 10 22 355 491 1695 1806 2364
Size: 10 12 333 136 1204 111 558
Fragments arranged by size:
1204 558 333 136 111 12 10
EagI C'GGCC_G
Cuts at: 0 10 22 1806 2364
Size: 10 12 1784 558
Fragments arranged by size:
1784 558 12 10
EarI CTCTTCn'nnn_
Cuts at: 0 1163 2120 2364
Size: 1163 957 244
Fragments arranged by size:
1163 957 244
EcoICRI GAG'CTC
Cuts at: 0 110 1047 2364
Size: 110 937 1317
Fragments arranged by size:
1317 937 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 232 320 810 1084 1187 1251 2364
Size: 217 15 88 490 274 103 64 1113
Fragments arranged by size:
1113 490 274 217 103 88 64 15
EcoO109I rG'GnC_Cy
Cuts at: 0 79 165 278 591 1436 2145 2146 2364
Size: 79 86 113 313 845 709 1 218
Fragments arranged by size:
845 709 313 218 113 86 79 1
EcoRI G'AATT_C
Cuts at: 0 2254 2364
Size: 2254 110
HaeII r_GCGC'y
Cuts at: 0 49 58 2364
Size: 49 9 2306
Fragments arranged by size:
2306 49 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 931 958 963 990 1639 1671
Size: 280 32 619 27 5 27 649 32
Cuts at: 1671 1981 2013 2314 2346 2364
Size: 310 32 301 32 18
Fragments arranged by size:
649 619 310 301 280 32 32 32
32 27 27 18 5
KpnI G_GTAC'C
Cuts at: 0 485 2364
Size: 485 1879
MluI A'CGCG_T
Cuts at: 0 1762 2364
Size: 1762 602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1754 2364
Size: 1754 610
MscI TGG'CCA
Cuts at: 0 493 1697 2364
Size: 493 1204 667
Fragments arranged by size:
1204 667 493
MslI CAynn'nnrTG
Cuts at: 0 385 499 934 1607 1777 2158 2364
Size: 385 114 435 673 170 381 206
Fragments arranged by size:
673 435 385 381 206 170 114
MspA1I CmG'CkG
Cuts at: 0 15 1569 1706 1805 1811 1832 2364
Size: 15 1554 137 99 6 21 532
Fragments arranged by size:
1554 532 137 99 21 15 6
NcoI C'CATG_G
Cuts at: 0 935 1079 1692 2364
Size: 935 144 613 672
Fragments arranged by size:
935 672 613 144
NotI GC'GGCC_GC
Cuts at: 0 10 1806 2364
Size: 10 1796 558
Fragments arranged by size:
1796 558 10
NsiI A_TGCA'T
Cuts at: 0 804 2364
Size: 804 1560
NspI r_CATG'y
Cuts at: 0 573 1782 2364
Size: 573 1209 582
Fragments arranged by size:
1209 582 573
PasI CC'CwG_GG
Cuts at: 0 1054 2364
Size: 1054 1310
PciI A'CATG_T
Cuts at: 0 569 2364
Size: 569 1795
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 739 813 2364
Size: 477 262 74 1551
Fragments arranged by size:
1551 477 262 74
PmlI CAC'GTG
Cuts at: 0 654 2364
Size: 654 1710
PpuMI rG'GwC_Cy
Cuts at: 0 1436 2364
Size: 1436 928
PspOMI G'GGCC_C
Cuts at: 0 79 2145 2364
Size: 79 2066 219
Fragments arranged by size:
2066 219 79
PstI C_TGCA'G
Cuts at: 0 1564 2364
Size: 1564 800
PvuII CAG'CTG
Cuts at: 0 1706 1832 2364
Size: 1706 126 532
Fragments arranged by size:
1706 532 126
SacI G_AGCT'C
Cuts at: 0 112 1049 2364
Size: 112 937 1315
Fragments arranged by size:
1315 937 112
SacII CC_GC'GG
Cuts at: 0 16 1812 2364
Size: 16 1796 552
Fragments arranged by size:
1796 552 16
SapI GCTCTTCn'nnn_
Cuts at: 0 2120 2364
Size: 2120 244
ScaI AGT'ACT
Cuts at: 0 266 1636 2083 2364
Size: 266 1370 447 281
Fragments arranged by size:
1370 447 281 266
SexAI A'CCwGG_T
Cuts at: 0 671 2364
Size: 671 1693
SfcI C'TryA_G
Cuts at: 0 764 1038 1560 2364
Size: 764 274 522 804
Fragments arranged by size:
804 764 522 274
SmaI CCC'GGG
Cuts at: 0 1543 2364
Size: 1543 821
SmlI C'TyrA_G
Cuts at: 0 320 1225 1243 2011 2364
Size: 320 905 18 768 353
Fragments arranged by size:
905 768 353 320 18
StyI C'CwwG_G
Cuts at: 0 935 1079 1280 1692 2141 2364
Size: 935 144 201 412 449 223
Fragments arranged by size:
935 449 412 223 201 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 511 2261 2364
Size: 511 1750 103
Fragments arranged by size:
1750 511 103
TatI w'GTAC_w
Cuts at: 0 264 1634 2081 2364
Size: 264 1370 447 283
Fragments arranged by size:
1370 447 283 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 335 367 445 477 1244 1276 1705 1737
Size: 335 32 78 32 767 32 429 32
Cuts at: 1737 2364
Size: 627
Fragments arranged by size:
767 627 429 335 78 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2065 2364
Size: 2065 299
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1329 1705 2115 2364
Size: 1329 376 410 249
Fragments arranged by size:
1329 410 376 249
XmaI C'CCGG_G
Cuts at: 0 1541 2364
Size: 1541 823
XmnI GAAnn'nnTTC
Cuts at: 0 373 2364
Size: 373 1991
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlfI
AloI AlwNI ApaI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BciVI BfrBI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I KpnI MluI MmeI MscI
MslI MspA1I NcoI NotI NsiI NspI PasI PciI
PflMI PmlI PpuMI PspOMI PstI PvuII SacI SacII
SapI ScaI SexAI SfcI SmaI SmlI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI ApaLI AscI AseI
AsiSI AvrII BbeI BcgI BclI BglII BlpI BmtI
BsaI BsaBI BsaHI BsaWI BsiWI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstZ17I Bsu36I ClaI CspCI DraI
DraIII EciI EcoNI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI NaeI NarI NdeI
NgoMIV NheI NruI PacI PfoI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XhoI ZraI