Restriction map for
pF1KB5623
(Linear) (Six-base) MAPSORT of: KIBB5623.seq Check: 4599 from: 1 to: 2937
KIBB5623.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1976 2937
Size: 1976 961
AclI AA'CG_TT
Cuts at: 0 84 2937
Size: 84 2853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1779 1859 2937
Size: 1779 80 1078
Fragments arranged by size:
1779 1078 80
AflII C'TTAA_G
Cuts at: 0 945 1170 2937
Size: 945 225 1767
Fragments arranged by size:
1767 945 225
AflIII A'CryG_T
Cuts at: 0 130 579 2937
Size: 130 449 2358
Fragments arranged by size:
2358 449 130
AleI CACnn'nnGTG
Cuts at: 0 262 2937
Size: 262 2675
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 2937
Size: 430 32 2475
Fragments arranged by size:
2475 430 32
AlwNI CAG_nnn'CTG
Cuts at: 0 282 2402 2596 2937
Size: 282 2120 194 341
Fragments arranged by size:
2120 341 282 194
ApaLI G'TGCA_C
Cuts at: 0 1063 2937
Size: 1063 1874
ApoI r'AATT_y
Cuts at: 0 378 1799 2359 2937
Size: 378 1421 560 578
Fragments arranged by size:
1421 578 560 378
AseI AT'TA_AT
Cuts at: 0 2353 2937
Size: 2353 584
AvrII C'CTAG_G
Cuts at: 0 2460 2937
Size: 2460 477
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 86 119 1960 1993 2937
Size: 86 33 1841 33 944
Fragments arranged by size:
1841 944 86 33 33
BamHI G'GATC_C
Cuts at: 0 771 2937
Size: 771 2166
BanI G'GyrC_C
Cuts at: 0 481 1976 2814 2937
Size: 481 1495 838 123
Fragments arranged by size:
1495 838 481 123
BanII G_rGCy'C
Cuts at: 0 308 719 1448 2937
Size: 308 411 729 1489
Fragments arranged by size:
1489 729 411 308
BciVI GTATCCnnnnn_n'
Cuts at: 0 540 1846 1893 2416 2637 2937
Size: 540 1306 47 523 221 300
Fragments arranged by size:
1306 540 523 300 221 47
Bme1580I G_kGCm'C
Cuts at: 0 1067 2937
Size: 1067 1870
BmrI ACTGGGnnnn_n'
Cuts at: 0 2165 2751 2800 2937
Size: 2165 586 49 137
Fragments arranged by size:
2165 586 137 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 414 1329 1673 2750 2937
Size: 405 9 915 344 1077 187
Fragments arranged by size:
1077 915 405 344 187 9
Bpu10I CC'TnA_GC
Cuts at: 0 371 2937
Size: 371 2566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 730 881 2937
Size: 72 658 151 2056
Fragments arranged by size:
2056 658 151 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 2296 2937
Size: 2296 641
BsaAI yAC'GTr
Cuts at: 0 1890 2937
Size: 1890 1047
BsaBI GATnn'nnATC
Cuts at: 0 208 2227 2937
Size: 208 2019 710
Fragments arranged by size:
2019 710 208
BsaWI w'CCGG_w
Cuts at: 0 2199 2937
Size: 2199 738
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2692 2722 2937
Size: 2692 30 215
Fragments arranged by size:
2692 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 2202 2889 2937
Size: 727 1475 687 48
Fragments arranged by size:
1475 727 687 48
BseYI C'CCAG_C
Cuts at: 0 293 2649 2724 2937
Size: 293 2356 75 213
Fragments arranged by size:
2356 293 213 75
BsiEI CG_ry'CG
Cuts at: 0 2882 2937
Size: 2882 55
BsiHKAI G_wGCw'C
Cuts at: 0 308 1067 1448 1706 2937
Size: 308 759 381 258 1231
Fragments arranged by size:
1231 759 381 308 258
BsmI GAATG_Cn'
Cuts at: 0 589 2937
Size: 589 2348
Bsp1286I G_dGCh'C
Cuts at: 0 308 719 1067 1448 1706 2937
Size: 308 411 348 381 258 1231
Fragments arranged by size:
1231 411 381 348 308 258
BspEI T'CCGG_A
Cuts at: 0 2199 2937
Size: 2199 738
BspHI T'CATG_A
Cuts at: 0 1049 2937
Size: 1049 1888
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1194 2937
Size: 1194 1743
BsrDI GCAATG_nn'
Cuts at: 0 40 2937
Size: 40 2897
BsrGI T'GTAC_A
Cuts at: 0 417 862 2937
Size: 417 445 2075
Fragments arranged by size:
2075 445 417
BstXI CCAn_nnnn'nTGG
Cuts at: 0 383 2937
Size: 383 2554
BstYI r'GATC_y
Cuts at: 0 203 771 1391 2916 2937
Size: 203 568 620 1525 21
Fragments arranged by size:
1525 620 568 203 21
BtgI C'CryG_G
Cuts at: 0 1110 2240 2937
Size: 1110 1130 697
Fragments arranged by size:
1130 1110 697
BtsI GCAGTG_nn'
Cuts at: 0 785 2014 2937
Size: 785 1229 923
Fragments arranged by size:
1229 923 785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 312 347 2937
Size: 312 35 2590
Fragments arranged by size:
2590 312 35
EaeI y'GGCC_r
Cuts at: 0 2613 2937
Size: 2613 324
EarI CTCTTCn'nnn_
Cuts at: 0 946 2937
Size: 946 1991
EcoICRI GAG'CTC
Cuts at: 0 306 1446 2937
Size: 306 1140 1491
Fragments arranged by size:
1491 1140 306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 414 1329 1673 1779 1859 2750 2937
Size: 405 9 915 344 106 80 891 187
Fragments arranged by size:
915 891 405 344 187 106 80 9
EcoO109I rG'GnC_Cy
Cuts at: 0 1296 1732 2167 2937
Size: 1296 436 435 770
Fragments arranged by size:
1296 770 436 435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 842 1844 2937
Size: 842 1002 1093
Fragments arranged by size:
1093 1002 842
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1417 1449 2194 2226 2295 2327 2590 2622
Size: 1417 32 745 32 69 32 263 32
Cuts at: 2622 2937
Size: 315
Fragments arranged by size:
1417 745 315 263 69 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1017 2495 2937
Size: 1017 1478 442
Fragments arranged by size:
1478 1017 442
KpnI G_GTAC'C
Cuts at: 0 1980 2937
Size: 1980 957
MluI A'CGCG_T
Cuts at: 0 130 2937
Size: 130 2807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1912 2548 2937
Size: 1912 636 389
Fragments arranged by size:
1912 636 389
MscI TGG'CCA
Cuts at: 0 2615 2937
Size: 2615 322
MslI CAynn'nnrTG
Cuts at: 0 241 262 1092 1150 1382 2269 2937
Size: 241 21 830 58 232 887 668
Fragments arranged by size:
887 830 668 241 232 58 21
MspA1I CmG'CkG
Cuts at: 0 2728 2937
Size: 2728 209
NcoI C'CATG_G
Cuts at: 0 1110 2240 2937
Size: 1110 1130 697
Fragments arranged by size:
1130 1110 697
NspI r_CATG'y
Cuts at: 0 583 1381 2937
Size: 583 798 1556
Fragments arranged by size:
1556 798 583
PasI CC'CwG_GG
Cuts at: 0 2712 2937
Size: 2712 225
PciI A'CATG_T
Cuts at: 0 579 2937
Size: 579 2358
PmlI CAC'GTG
Cuts at: 0 1890 2937
Size: 1890 1047
PpuMI rG'GwC_Cy
Cuts at: 0 1732 2937
Size: 1732 1205
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 2937
Size: 1147 32 1758
Fragments arranged by size:
1758 1147 32
PstI C_TGCA'G
Cuts at: 0 1572 2937
Size: 1572 1365
PvuII CAG'CTG
Cuts at: 0 2728 2937
Size: 2728 209
SacI G_AGCT'C
Cuts at: 0 308 1448 2937
Size: 308 1140 1489
Fragments arranged by size:
1489 1140 308
ScaI AGT'ACT
Cuts at: 0 707 1165 2937
Size: 707 458 1772
Fragments arranged by size:
1772 707 458
SexAI A'CCwGG_T
Cuts at: 0 275 1959 2937
Size: 275 1684 978
Fragments arranged by size:
1684 978 275
SfcI C'TryA_G
Cuts at: 0 1568 2937
Size: 1568 1369
SmlI C'TyrA_G
Cuts at: 0 51 709 896 945 1170 2937
Size: 51 658 187 49 225 1767
Fragments arranged by size:
1767 658 225 187 51 49
SpeI A'CTAG_T
Cuts at: 0 2152 2937
Size: 2152 785
SphI G_CATG'C
Cuts at: 0 1381 2937
Size: 1381 1556
StyI C'CwwG_G
Cuts at: 0 719 791 1110 1997 2240 2460 2937
Size: 719 72 319 887 243 220 477
Fragments arranged by size:
887 719 477 319 243 220 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 1525 2030 2784 2896 2937
Size: 231 1294 505 754 112 41
Fragments arranged by size:
1294 754 505 231 112 41
TatI w'GTAC_w
Cuts at: 0 417 705 763 862 1163 1196 2676 2937
Size: 417 288 58 99 301 33 1480 261
Fragments arranged by size:
1480 417 301 288 261 99 58 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 942 974 1230 1262 2937
Size: 942 32 256 32 1675
Fragments arranged by size:
1675 942 256 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1197 1530 2937
Size: 1197 333 1407
Fragments arranged by size:
1407 1197 333
XmnI GAAnn'nnTTC
Cuts at: 0 1081 2937
Size: 1081 1856
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AleI AloI AlwNI
ApaLI ApoI AseI AvrII BaeI BamHI BanI BanII
BciVI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstXI BstYI
BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI Hin4I HincII KpnI MluI MmeI MscI MslI
MspA1I NcoI NspI PasI PciI PmlI PpuMI PsrI
PstI PvuII SacI ScaI SexAI SfcI SmlI SpeI
SphI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AlfI ApaI
AscI AsiSI AvaI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaHI
BsgI BsiWI BsmBI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI ZraI