Restriction map for
pF1KB5631
(Linear) (Six-base) MAPSORT of: KIBB5631.seq Check: 3398 from: 1 to: 3108
KIBB5631.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1548 1718 3108
Size: 1548 170 1390
Fragments arranged by size:
1548 1390 170
AccI GT'mk_AC
Cuts at: 0 119 833 1320 3108
Size: 119 714 487 1788
Fragments arranged by size:
1788 714 487 119
Acc65I G'GTAC_C
Cuts at: 0 288 3108
Size: 288 2820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1496 2684 3016 3065 3108
Size: 1496 1188 332 49 43
Fragments arranged by size:
1496 1188 332 49 43
AfeI AGC'GCT
Cuts at: 0 1984 3108
Size: 1984 1124
AflII C'TTAA_G
Cuts at: 0 1566 3108
Size: 1566 1542
AflIII A'CryG_T
Cuts at: 0 128 437 3108
Size: 128 309 2671
Fragments arranged by size:
2671 309 128
AleI CACnn'nnGTG
Cuts at: 0 1486 3108
Size: 1486 1622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 65 1943 3108
Size: 65 1878 1165
Fragments arranged by size:
1878 1165 65
AlwNI CAG_nnn'CTG
Cuts at: 0 1814 2692 3108
Size: 1814 878 416
Fragments arranged by size:
1814 878 416
ApoI r'AATT_y
Cuts at: 0 331 355 373 841 973 1675 2448 2827
Size: 331 24 18 468 132 702 773 379
Cuts at: 2827 3108
Size: 281
Fragments arranged by size:
773 702 468 379 331 281 132 24
18
AseI AT'TA_AT
Cuts at: 0 299 3108
Size: 299 2809
AvaI C'yCGr_G
Cuts at: 0 470 2741 3108
Size: 470 2271 367
Fragments arranged by size:
2271 470 367
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 205 238 272 305 3108
Size: 205 33 34 33 2803
Fragments arranged by size:
2803 205 34 33 33
BamHI G'GATC_C
Cuts at: 0 2297 2811 3108
Size: 2297 514 297
Fragments arranged by size:
2297 514 297
BanI G'GyrC_C
Cuts at: 0 288 808 991 1703 2894 3108
Size: 288 520 183 712 1191 214
Fragments arranged by size:
1191 712 520 288 214 183
BanII G_rGCy'C
Cuts at: 0 1386 2537 3108
Size: 1386 1151 571
Fragments arranged by size:
1386 1151 571
BbsI GAAGACnn'nnnn_
Cuts at: 0 438 1836 3108
Size: 438 1398 1272
Fragments arranged by size:
1398 1272 438
BbvCI CC'TCA_GC
Cuts at: 0 2984 3108
Size: 2984 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2269 2303 3108
Size: 2269 34 805
Fragments arranged by size:
2269 805 34
BglII A'GATC_T
Cuts at: 0 685 3108
Size: 685 2423
Bme1580I G_kGCm'C
Cuts at: 0 3092 3108
Size: 3092 16
BmgBI CAC'GTC
Cuts at: 0 129 1488 1969 3108
Size: 129 1359 481 1139
Fragments arranged by size:
1359 1139 481 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 476 1168 1863 3108
Size: 476 692 695 1245
Fragments arranged by size:
1245 695 692 476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 406 3108
Size: 406 2702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 3108
Size: 710 2398
Bpu10I CC'TnA_GC
Cuts at: 0 2963 2984 3108
Size: 2963 21 124
Fragments arranged by size:
2963 124 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 376 3108
Size: 176 200 2732
Fragments arranged by size:
2732 200 176
BsaBI GATnn'nnATC
Cuts at: 0 1129 3108
Size: 1129 1979
BsaWI w'CCGG_w
Cuts at: 0 2891 3108
Size: 2891 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 101 131 1732 1762 1882 1912 2102 2132
Size: 101 30 1601 30 120 30 190 30
Cuts at: 2132 3108
Size: 976
Fragments arranged by size:
1601 976 190 120 101 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 478 2456 3001 3108
Size: 127 351 1978 545 107
Fragments arranged by size:
1978 545 351 127 107
BseYI C'CCAG_C
Cuts at: 0 136 2643 3108
Size: 136 2507 465
Fragments arranged by size:
2507 465 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 1696 2457 3108
Size: 20 1676 761 651
Fragments arranged by size:
1676 761 651 20
BsiHKAI G_wGCw'C
Cuts at: 0 1475 2537 3108
Size: 1475 1062 571
Fragments arranged by size:
1475 1062 571
BsmI GAATG_Cn'
Cuts at: 0 1050 1208 3108
Size: 1050 158 1900
Fragments arranged by size:
1900 1050 158
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1526 3108
Size: 1526 1582
Bsp1286I G_dGCh'C
Cuts at: 0 1386 1475 2537 3092 3108
Size: 1386 89 1062 555 16
Fragments arranged by size:
1386 1062 555 89 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1548 1718 3108
Size: 1548 170 1390
Fragments arranged by size:
1548 1390 170
BsrBI CCG'CTC
Cuts at: 0 2123 3108
Size: 2123 985
BsrDI GCAATG_nn'
Cuts at: 0 812 1109 1553 1925 2285 3108
Size: 812 297 444 372 360 823
Fragments arranged by size:
823 812 444 372 360 297
BsrFI r'CCGG_y
Cuts at: 0 2383 2732 3108
Size: 2383 349 376
Fragments arranged by size:
2383 376 349
BssSI C'ACGA_G
Cuts at: 0 1955 3108
Size: 1955 1153
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1710 3108
Size: 1710 1398
BstBI TT'CG_AA
Cuts at: 0 2366 3108
Size: 2366 742
BstXI CCAn_nnnn'nTGG
Cuts at: 0 988 3108
Size: 988 2120
BstYI r'GATC_y
Cuts at: 0 685 1241 2297 2811 2970 3002 3108
Size: 685 556 1056 514 159 32 106
Fragments arranged by size:
1056 685 556 514 159 106 32
BtgI C'CryG_G
Cuts at: 0 19 1941 3006 3092 3108
Size: 19 1922 1065 86 16
Fragments arranged by size:
1922 1065 86 19 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2294 3108
Size: 2294 814
BtsI GCAGTG_nn'
Cuts at: 0 37 1500 3108
Size: 37 1463 1608
Fragments arranged by size:
1608 1463 37
ClaI AT'CG_AT
Cuts at: 0 1014 3108
Size: 1014 2094
DraI TTT'AAA
Cuts at: 0 336 923 3108
Size: 336 587 2185
Fragments arranged by size:
2185 587 336
EaeI y'GGCC_r
Cuts at: 0 2730 3108
Size: 2730 378
EarI CTCTTCn'nnn_
Cuts at: 0 726 1103 1115 1609 2156 3108
Size: 726 377 12 494 547 952
Fragments arranged by size:
952 726 547 494 377 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2402 3108
Size: 2402 706
EcoICRI GAG'CTC
Cuts at: 0 2535 3108
Size: 2535 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1496 2684 3016 3065 3108
Size: 710 786 1188 332 49 43
Fragments arranged by size:
1188 786 710 332 49 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1452 3108
Size: 1452 1656
EcoRI G'AATT_C
Cuts at: 0 841 973 3108
Size: 841 132 2135
Fragments arranged by size:
2135 841 132
EcoRV GAT'ATC
Cuts at: 0 2080 3108
Size: 2080 1028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2494 3108
Size: 2494 614
HaeII r_GCGC'y
Cuts at: 0 1986 3108
Size: 1986 1122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1226 1258 1345 1377 1732 1764 1882 1914
Size: 1226 32 87 32 355 32 118 32
Cuts at: 1914 1915 1947 3108
Size: 1 32 1161
Fragments arranged by size:
1226 1161 355 118 87 32 32 32
32 32 1
HindIII A'AGCT_T
Cuts at: 0 1396 3108
Size: 1396 1712
KpnI G_GTAC'C
Cuts at: 0 292 3108
Size: 292 2816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1181 3108
Size: 1174 7 1927
Fragments arranged by size:
1927 1174 7
MslI CAynn'nnrTG
Cuts at: 0 1336 1486 3108
Size: 1336 150 1622
Fragments arranged by size:
1622 1336 150
MspA1I CmG'CkG
Cuts at: 0 630 806 2931 3108
Size: 630 176 2125 177
Fragments arranged by size:
2125 630 177 176
NcoI C'CATG_G
Cuts at: 0 3092 3108
Size: 3092 16
NdeI CA'TA_TG
Cuts at: 0 1026 3108
Size: 1026 2082
NspI r_CATG'y
Cuts at: 0 946 2530 2643 3108
Size: 946 1584 113 465
Fragments arranged by size:
1584 946 465 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 648 1419 1814 3108
Size: 648 771 395 1294
Fragments arranged by size:
1294 771 648 395
PsiI TTA'TAA
Cuts at: 0 185 2944 3108
Size: 185 2759 164
Fragments arranged by size:
2759 185 164
PstI C_TGCA'G
Cuts at: 0 44 1938 3108
Size: 44 1894 1170
Fragments arranged by size:
1894 1170 44
PvuII CAG'CTG
Cuts at: 0 630 2931 3108
Size: 630 2301 177
Fragments arranged by size:
2301 630 177
SacI G_AGCT'C
Cuts at: 0 2537 3108
Size: 2537 571
SapI GCTCTTCn'nnn_
Cuts at: 0 726 3108
Size: 726 2382
SfcI C'TryA_G
Cuts at: 0 40 741 936 965 1934 2401 2424 2755
Size: 40 701 195 29 969 467 23 331
Cuts at: 2755 3108
Size: 353
Fragments arranged by size:
969 701 467 353 331 195 40 29
23
SmlI C'TyrA_G
Cuts at: 0 191 391 470 1566 3108
Size: 191 200 79 1096 1542
Fragments arranged by size:
1542 1096 200 191 79
SphI G_CATG'C
Cuts at: 0 2643 3108
Size: 2643 465
StyI C'CwwG_G
Cuts at: 0 528 2457 2775 3092 3108
Size: 528 1929 318 317 16
Fragments arranged by size:
1929 528 318 317 16
SwaI ATTT'AAAT
Cuts at: 0 336 3108
Size: 336 2772
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2009 3108
Size: 2009 1099
TatI w'GTAC_w
Cuts at: 0 395 2913 3108
Size: 395 2518 195
Fragments arranged by size:
2518 395 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3002 3034 3108
Size: 3002 32 74
Fragments arranged by size:
3002 74 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3090 3108
Size: 3090 18
XhoI C'TCGA_G
Cuts at: 0 470 3108
Size: 470 2638
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApoI AseI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI HaeII Hin4I HindIII
KpnI MmeI MslI MspA1I NcoI NdeI NspI PflMI
PsiI PstI PvuII SacI SapI SfcI SmlI SphI
StyI SwaI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AclI AgeI AhdI AloI ApaI ApaLI AscI
AsiSI AvrII BbeI BciVI BclI BfrBI BglI BlpI
BmtI BsaI BsaAI BsaHI BsiEI BsiWI BspEI BspHI
BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII DrdI
EagI EcoO109I FseI FspI FspAI HincII HpaI KasI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
Tth111I XbaI XmaI XmnI ZraI