Restriction map for pF1KB5634
 (Linear) (Six-base) MAPSORT of: KIBB5634.seq  Check: 4966  from: 1  to: 2352



KIBB5634.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      67    2049    2352 
   Size:         67    1982     303

  Fragments arranged by size: 

                1982     303      67
 
AccI GT'mk_AC

Cuts at:      0    1310    2352 
   Size:       1310    1042
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     824    1162    2352 
   Size:        601     223     338    1190

  Fragments arranged by size: 

                1190     601     338     223
 
AflIII A'CryG_T

Cuts at:      0     302    2352 
   Size:        302    2050
 
AleI CACnn'nnGTG

Cuts at:      0     814     826    1093    2352 
   Size:        814      12     267    1259

  Fragments arranged by size: 

                1259     814     267      12
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1918    2352 
   Size:       1918     434
 
ApaI G_GGCC'C

Cuts at:      0    2105    2352 
   Size:       2105     247
 
ApoI r'AATT_y

Cuts at:      0    1543    2352 
   Size:       1543     809
 
AvaI C'yCGr_G

Cuts at:      0     714    2104    2333    2352 
   Size:        714    1390     229      19

  Fragments arranged by size: 

                1390     714     229      19
 
BanI G'GyrC_C

Cuts at:      0      34     128     820    1699    2239    2352 
   Size:         34      94     692     879     540     113

  Fragments arranged by size: 

                 879     692     540     113      94      34
 
BanII G_rGCy'C

Cuts at:      0     835     863    1051    1071    1106    2105    2206    2352 
   Size:        835      28     188      20      35     999     101     146

  Fragments arranged by size: 

                 999     835     188     146     101      35      28      20
            
 
BbeI G_GCGC'C

Cuts at:      0      38     132    2352 
   Size:         38      94    2220

  Fragments arranged by size: 

                2220      94      38
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     267     812    2352 
   Size:        267     545    1540

  Fragments arranged by size: 

                1540     545     267
 
BbvCI CC'TCA_GC

Cuts at:      0    2227    2352 
   Size:       2227     125
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1210    2352 
   Size:       1210    1142
 
BclI T'GATC_A

Cuts at:      0    1838    2352 
   Size:       1838     514
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2022    2076    2352 
   Size:       2022      54     276

  Fragments arranged by size: 

                2022     276      54
 
BglII A'GATC_T

Cuts at:      0    1496    2352 
   Size:       1496     856
 
BlpI GC'TnA_GC

Cuts at:      0    2312    2352 
   Size:       2312      40
 
Bme1580I G_kGCm'C

Cuts at:      0     721     823    1702    2094    2105    2242    2352 
   Size:        721     102     879     392      11     137     110

  Fragments arranged by size: 

                 879     721     392     137     110     102      11
 
BmgBI CAC'GTC

Cuts at:      0     303     429    2262    2352 
   Size:        303     126    1833      90

  Fragments arranged by size: 

                1833     303     126      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2295    2352 
   Size:       2295      57
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     354    2352 
   Size:        354    1998
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    2352 
   Size:        412    1940
 
Bpu10I CC'TnA_GC

Cuts at:      0    2227    2352 
   Size:       2227     125
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     545    2352 
   Size:        545    1807
 
BsaHI Gr'CG_yC

Cuts at:      0      35     129    2036    2352 
   Size:         35      94    1907     316

  Fragments arranged by size: 

                1907     316      94      35
 
BsaWI w'CCGG_w

Cuts at:      0     869    1871    2352 
   Size:        869    1002     481

  Fragments arranged by size: 

                1002     869     481
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     248     278    2188    2218    2332    2352 
   Size:        248      30    1910      30     114      20

  Fragments arranged by size: 

                1910     248     114      30      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10    1954    2352 
   Size:         10    1944     398

  Fragments arranged by size: 

                1944     398      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2083    2167    2352 
   Size:       2083      84     185

  Fragments arranged by size: 

                2083     185      84
 
BsiHKAI G_wGCw'C

Cuts at:      0    1988    2352 
   Size:       1988     364
 
BsmI GAATG_Cn'

Cuts at:      0     240    2352 
   Size:        240    2112
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     293    2352 
   Size:        293    2059
 
Bsp1286I G_dGCh'C

Cuts at:      0     721     823     835     863    1051    1071    1106    1702 
   Size:        721     102      12      28     188      20      35     596

Cuts at:   1702    1988    2094    2105    2206    2242    2352 
   Size:        286     106      11     101      36     110

  Fragments arranged by size: 

                 721     596     286     188     110     106     102     101
                  36      35      28      20      12      11
 
BspEI T'CCGG_A

Cuts at:      0    1871    2352 
   Size:       1871     481
 
BspHI T'CATG_A

Cuts at:      0      11     423    2352 
   Size:         11     412    1929

  Fragments arranged by size: 

                1929     412      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      67     254    2049    2352 
   Size:         67     187    1795     303

  Fragments arranged by size: 

                1795     303     187      67
 
BsrFI r'CCGG_y

Cuts at:      0     125    2352 
   Size:        125    2227
 
BssHII G'CGCG_C

Cuts at:      0    1886    2352 
   Size:       1886     466
 
BssSI C'ACGA_G

Cuts at:      0    1915    2352 
   Size:       1915     437
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      79     529    2352 
   Size:         79     450    1823

  Fragments arranged by size: 

                1823     450      79
 
BstEII G'GTnAC_C

Cuts at:      0    1926    2352 
   Size:       1926     426
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     732    1347    2352 
   Size:        732     615    1005

  Fragments arranged by size: 

                1005     732     615
 
BstYI r'GATC_y

Cuts at:      0    1496    1910    2352 
   Size:       1496     414     442

  Fragments arranged by size: 

                1496     442     414
 
BtgI C'CryG_G

Cuts at:      0    1055    1184    1476    1661    1715    2001    2020    2352 
   Size:       1055     129     292     185      54     286      19     332

  Fragments arranged by size: 

                1055     332     292     286     185     129      54      19
            
 
BtsI GCAGTG_nn'

Cuts at:      0      62      71     353     530    1805    2352 
   Size:         62       9     282     177    1275     547

  Fragments arranged by size: 

                1275     547     282     177      62       9
 
ClaI AT'CG_AT

Cuts at:      0     605    2352 
   Size:        605    1747
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1306    2352 
   Size:       1306    1046
 
EaeI y'GGCC_r

Cuts at:      0     722    1181    1479    1598    1718    1769    2352 
   Size:        722     459     298     119     120      51     583

  Fragments arranged by size: 

                 722     583     459     298     120     119      51
 
EarI CTCTTCn'nnn_

Cuts at:      0     167    2352 
   Size:        167    2185
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2161    2352 
   Size:       2161     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     601     824    1162    2352 
   Size:        412     189     223     338    1190

  Fragments arranged by size: 

                1190     412     338     223     189
 
EcoO109I rG'GnC_Cy

Cuts at:      0     500     699     849    2101    2290    2352 
   Size:        500     199     150    1252     189      62

  Fragments arranged by size: 

                1252     500     199     189     150      62
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1668    2352 
   Size:       1668     684
 
FspI TGC'GCA

Cuts at:      0    2057    2352 
   Size:       2057     295
 
HaeII r_GCGC'y

Cuts at:      0      38     132    2352 
   Size:         38      94    2220

  Fragments arranged by size: 

                2220      94      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     544     576     608     640    1058    1090    1208    1240 
   Size:        544      32      32      32     418      32     118      32

Cuts at:   1240    1674    1706    2352 
   Size:        434      32     646

  Fragments arranged by size: 

                 646     544     434     418     118      32      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     639    2352 
   Size:        639    1713
 
KasI G'GCGC_C

Cuts at:      0      34     128    2352 
   Size:         34      94    2224

  Fragments arranged by size: 

                2224      94      34
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2276    2352 
   Size:       2276      76
 
MscI TGG'CCA

Cuts at:      0    1183    1481    2352 
   Size:       1183     298     871

  Fragments arranged by size: 

                1183     871     298
 
MslI CAynn'nnrTG

Cuts at:      0     167     514     814     826    1093    1834    2352 
   Size:        167     347     300      12     267     741     518

  Fragments arranged by size: 

                 741     518     347     300     267     167      12
 
MspA1I CmG'CkG

Cuts at:      0    1057    1140    1485    1923    2003    2022    2352 
   Size:       1057      83     345     438      80      19     330

  Fragments arranged by size: 

                1057     438     345     330      83      80      19
 
NarI GG'CG_CC

Cuts at:      0      35     129    2352 
   Size:         35      94    2223

  Fragments arranged by size: 

                2223      94      35
 
NcoI C'CATG_G

Cuts at:      0    1184    1476    2352 
   Size:       1184     292     876

  Fragments arranged by size: 

                1184     876     292
 
PasI CC'CwG_GG

Cuts at:      0     696    2235    2352 
   Size:        696    1539     117

  Fragments arranged by size: 

                1539     696     117
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1800    2352 
   Size:       1800     552
 
PpuMI rG'GwC_Cy

Cuts at:      0     500     849    2290    2352 
   Size:        500     349    1441      62

  Fragments arranged by size: 

                1441     500     349      62
 
PshAI GACnn'nnGTC

Cuts at:      0     505    2352 
   Size:        505    1847
 
PspOMI G'GGCC_C

Cuts at:      0    2101    2352 
   Size:       2101     251
 
PstI C_TGCA'G

Cuts at:      0    1496    2352 
   Size:       1496     856
 
PvuII CAG'CTG

Cuts at:      0    1140    1485    1923    2352 
   Size:       1140     345     438     429

  Fragments arranged by size: 

                1140     438     429     345
 
SacII CC_GC'GG

Cuts at:      0    1058    2004    2023    2352 
   Size:       1058     946      19     329

  Fragments arranged by size: 

                1058     946     329      19
 
SanDI GG'GwC_CC

Cuts at:      0    2290    2352 
   Size:       2290      62
 
SfcI C'TryA_G

Cuts at:      0    1492    2352 
   Size:       1492     860
 
SfoI GGC'GCC

Cuts at:      0      36     130    2352 
   Size:         36      94    2222

  Fragments arranged by size: 

                2222      94      36
 
SgrAI Cr'CCGG_yG

Cuts at:      0     125    2352 
   Size:        125    2227
 
SmaI CCC'GGG

Cuts at:      0    2106    2335    2352 
   Size:       2106     229      17

  Fragments arranged by size: 

                2106     229      17
 
StyI C'CwwG_G

Cuts at:      0    1184    1476    1643    2352 
   Size:       1184     292     167     709

  Fragments arranged by size: 

                1184     709     292     167
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     118     136     662     790    2352 
   Size:        118      18     526     128    1562

  Fragments arranged by size: 

                1562     526     128     118      18
 
TatI w'GTAC_w

Cuts at:      0     785     881    2352 
   Size:        785      96    1471

  Fragments arranged by size: 

                1471     785      96
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     174     206     248     280    2352 
   Size:        174      32      42      32    2072

  Fragments arranged by size: 

                2072     174      42      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318     969    2352 
   Size:        318     651    1383

  Fragments arranged by size: 

                1383     651     318
 
XmaI C'CCGG_G

Cuts at:      0    2104    2333    2352 
   Size:       2104     229      19

  Fragments arranged by size: 

                2104     229      19
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI     ApaI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrFI   BssHII
    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     ClaI
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI     FspI
    HaeII    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     PasI    PflMI    PpuMI    PshAI   PspOMI     PstI
    PvuII    SacII    SanDI     SfcI     SfoI    SgrAI     SmaI     StyI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BfrBI     BmtI    BpuEI    BsaAI    BsaBI    BseYI    BsiEI
    BsiWI    BsrBI    BsrDI    BsrGI    BstBI  BstZ17I   Bsu36I    BtgZI
    CspCI     DraI   DraIII     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI    FspAI   HincII     HpaI     KpnI     MfeI     MluI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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