(Linear) (Six-base) MAPSORT of: KIBB5634.seq Check: 4966 from: 1 to: 2352 KIBB5634.orf With 182 enzymes: SgfI * February 28, 2007 11:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 67 2049 2352 Size: 67 1982 303 Fragments arranged by size: 1982 303 67 AccI GT'mk_AC Cuts at: 0 1310 2352 Size: 1310 1042 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 824 1162 2352 Size: 601 223 338 1190 Fragments arranged by size: 1190 601 338 223 AflIII A'CryG_T Cuts at: 0 302 2352 Size: 302 2050 AleI CACnn'nnGTG Cuts at: 0 814 826 1093 2352 Size: 814 12 267 1259 Fragments arranged by size: 1259 814 267 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1918 2352 Size: 1918 434 ApaI G_GGCC'C Cuts at: 0 2105 2352 Size: 2105 247 ApoI r'AATT_y Cuts at: 0 1543 2352 Size: 1543 809 AvaI C'yCGr_G Cuts at: 0 714 2104 2333 2352 Size: 714 1390 229 19 Fragments arranged by size: 1390 714 229 19 BanI G'GyrC_C Cuts at: 0 34 128 820 1699 2239 2352 Size: 34 94 692 879 540 113 Fragments arranged by size: 879 692 540 113 94 34 BanII G_rGCy'C Cuts at: 0 835 863 1051 1071 1106 2105 2206 2352 Size: 835 28 188 20 35 999 101 146 Fragments arranged by size: 999 835 188 146 101 35 28 20 BbeI G_GCGC'C Cuts at: 0 38 132 2352 Size: 38 94 2220 Fragments arranged by size: 2220 94 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 267 812 2352 Size: 267 545 1540 Fragments arranged by size: 1540 545 267 BbvCI CC'TCA_GC Cuts at: 0 2227 2352 Size: 2227 125 BciVI GTATCCnnnnn_n' Cuts at: 0 1210 2352 Size: 1210 1142 BclI T'GATC_A Cuts at: 0 1838 2352 Size: 1838 514 BglI GCCn_nnn'nGGC Cuts at: 0 2022 2076 2352 Size: 2022 54 276 Fragments arranged by size: 2022 276 54 BglII A'GATC_T Cuts at: 0 1496 2352 Size: 1496 856 BlpI GC'TnA_GC Cuts at: 0 2312 2352 Size: 2312 40 Bme1580I G_kGCm'C Cuts at: 0 721 823 1702 2094 2105 2242 2352 Size: 721 102 879 392 11 137 110 Fragments arranged by size: 879 721 392 137 110 102 11 BmgBI CAC'GTC Cuts at: 0 303 429 2262 2352 Size: 303 126 1833 90 Fragments arranged by size: 1833 303 126 90 BmrI ACTGGGnnnn_n' Cuts at: 0 2295 2352 Size: 2295 57 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 354 2352 Size: 354 1998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 2352 Size: 412 1940 Bpu10I CC'TnA_GC Cuts at: 0 2227 2352 Size: 2227 125 BsaI GGTCTCn'nnnn_ Cuts at: 0 545 2352 Size: 545 1807 BsaHI Gr'CG_yC Cuts at: 0 35 129 2036 2352 Size: 35 94 1907 316 Fragments arranged by size: 1907 316 94 35 BsaWI w'CCGG_w Cuts at: 0 869 1871 2352 Size: 869 1002 481 Fragments arranged by size: 1002 869 481 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 248 278 2188 2218 2332 2352 Size: 248 30 1910 30 114 20 Fragments arranged by size: 1910 248 114 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 1954 2352 Size: 10 1944 398 Fragments arranged by size: 1944 398 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2083 2167 2352 Size: 2083 84 185 Fragments arranged by size: 2083 185 84 BsiHKAI G_wGCw'C Cuts at: 0 1988 2352 Size: 1988 364 BsmI GAATG_Cn' Cuts at: 0 240 2352 Size: 240 2112 BsmBI CGTCTCn'nnnn_ Cuts at: 0 293 2352 Size: 293 2059 Bsp1286I G_dGCh'C Cuts at: 0 721 823 835 863 1051 1071 1106 1702 Size: 721 102 12 28 188 20 35 596 Cuts at: 1702 1988 2094 2105 2206 2242 2352 Size: 286 106 11 101 36 110 Fragments arranged by size: 721 596 286 188 110 106 102 101 36 35 28 20 12 11 BspEI T'CCGG_A Cuts at: 0 1871 2352 Size: 1871 481 BspHI T'CATG_A Cuts at: 0 11 423 2352 Size: 11 412 1929 Fragments arranged by size: 1929 412 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 67 254 2049 2352 Size: 67 187 1795 303 Fragments arranged by size: 1795 303 187 67 BsrFI r'CCGG_y Cuts at: 0 125 2352 Size: 125 2227 BssHII G'CGCG_C Cuts at: 0 1886 2352 Size: 1886 466 BssSI C'ACGA_G Cuts at: 0 1915 2352 Size: 1915 437 BstAPI GCAn_nnn'nTGC Cuts at: 0 79 529 2352 Size: 79 450 1823 Fragments arranged by size: 1823 450 79 BstEII G'GTnAC_C Cuts at: 0 1926 2352 Size: 1926 426 BstXI CCAn_nnnn'nTGG Cuts at: 0 732 1347 2352 Size: 732 615 1005 Fragments arranged by size: 1005 732 615 BstYI r'GATC_y Cuts at: 0 1496 1910 2352 Size: 1496 414 442 Fragments arranged by size: 1496 442 414 BtgI C'CryG_G Cuts at: 0 1055 1184 1476 1661 1715 2001 2020 2352 Size: 1055 129 292 185 54 286 19 332 Fragments arranged by size: 1055 332 292 286 185 129 54 19 BtsI GCAGTG_nn' Cuts at: 0 62 71 353 530 1805 2352 Size: 62 9 282 177 1275 547 Fragments arranged by size: 1275 547 282 177 62 9 ClaI AT'CG_AT Cuts at: 0 605 2352 Size: 605 1747 DrdI GACnn_nn'nnGTC Cuts at: 0 1306 2352 Size: 1306 1046 EaeI y'GGCC_r Cuts at: 0 722 1181 1479 1598 1718 1769 2352 Size: 722 459 298 119 120 51 583 Fragments arranged by size: 722 583 459 298 120 119 51 EarI CTCTTCn'nnn_ Cuts at: 0 167 2352 Size: 167 2185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2161 2352 Size: 2161 191 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 601 824 1162 2352 Size: 412 189 223 338 1190 Fragments arranged by size: 1190 412 338 223 189 EcoO109I rG'GnC_Cy Cuts at: 0 500 699 849 2101 2290 2352 Size: 500 199 150 1252 189 62 Fragments arranged by size: 1252 500 199 189 150 62 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1668 2352 Size: 1668 684 FspI TGC'GCA Cuts at: 0 2057 2352 Size: 2057 295 HaeII r_GCGC'y Cuts at: 0 38 132 2352 Size: 38 94 2220 Fragments arranged by size: 2220 94 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 544 576 608 640 1058 1090 1208 1240 Size: 544 32 32 32 418 32 118 32 Cuts at: 1240 1674 1706 2352 Size: 434 32 646 Fragments arranged by size: 646 544 434 418 118 32 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 639 2352 Size: 639 1713 KasI G'GCGC_C Cuts at: 0 34 128 2352 Size: 34 94 2224 Fragments arranged by size: 2224 94 34 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2276 2352 Size: 2276 76 MscI TGG'CCA Cuts at: 0 1183 1481 2352 Size: 1183 298 871 Fragments arranged by size: 1183 871 298 MslI CAynn'nnrTG Cuts at: 0 167 514 814 826 1093 1834 2352 Size: 167 347 300 12 267 741 518 Fragments arranged by size: 741 518 347 300 267 167 12 MspA1I CmG'CkG Cuts at: 0 1057 1140 1485 1923 2003 2022 2352 Size: 1057 83 345 438 80 19 330 Fragments arranged by size: 1057 438 345 330 83 80 19 NarI GG'CG_CC Cuts at: 0 35 129 2352 Size: 35 94 2223 Fragments arranged by size: 2223 94 35 NcoI C'CATG_G Cuts at: 0 1184 1476 2352 Size: 1184 292 876 Fragments arranged by size: 1184 876 292 PasI CC'CwG_GG Cuts at: 0 696 2235 2352 Size: 696 1539 117 Fragments arranged by size: 1539 696 117 PflMI CCAn_nnn'nTGG Cuts at: 0 1800 2352 Size: 1800 552 PpuMI rG'GwC_Cy Cuts at: 0 500 849 2290 2352 Size: 500 349 1441 62 Fragments arranged by size: 1441 500 349 62 PshAI GACnn'nnGTC Cuts at: 0 505 2352 Size: 505 1847 PspOMI G'GGCC_C Cuts at: 0 2101 2352 Size: 2101 251 PstI C_TGCA'G Cuts at: 0 1496 2352 Size: 1496 856 PvuII CAG'CTG Cuts at: 0 1140 1485 1923 2352 Size: 1140 345 438 429 Fragments arranged by size: 1140 438 429 345 SacII CC_GC'GG Cuts at: 0 1058 2004 2023 2352 Size: 1058 946 19 329 Fragments arranged by size: 1058 946 329 19 SanDI GG'GwC_CC Cuts at: 0 2290 2352 Size: 2290 62 SfcI C'TryA_G Cuts at: 0 1492 2352 Size: 1492 860 SfoI GGC'GCC Cuts at: 0 36 130 2352 Size: 36 94 2222 Fragments arranged by size: 2222 94 36 SgrAI Cr'CCGG_yG Cuts at: 0 125 2352 Size: 125 2227 SmaI CCC'GGG Cuts at: 0 2106 2335 2352 Size: 2106 229 17 Fragments arranged by size: 2106 229 17 StyI C'CwwG_G Cuts at: 0 1184 1476 1643 2352 Size: 1184 292 167 709 Fragments arranged by size: 1184 709 292 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 118 136 662 790 2352 Size: 118 18 526 128 1562 Fragments arranged by size: 1562 526 128 118 18 TatI w'GTAC_w Cuts at: 0 785 881 2352 Size: 785 96 1471 Fragments arranged by size: 1471 785 96 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 174 206 248 280 2352 Size: 174 32 42 32 2072 Fragments arranged by size: 2072 174 42 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 318 969 2352 Size: 318 651 1383 Fragments arranged by size: 1383 651 318 XmaI C'CCGG_G Cuts at: 0 2104 2333 2352 Size: 2104 229 19 Fragments arranged by size: 2104 229 19 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DrdI EaeI EarI EciI Eco57MI EcoO109I FalI FspI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI NcoI PasI PflMI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SfcI SfoI SgrAI SmaI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BfrBI BmtI BpuEI BsaAI BsaBI BseYI BsiEI BsiWI BsrBI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HincII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI