Restriction map for
pF1KB5634
(Linear) (Six-base) MAPSORT of: KIBB5634.seq Check: 4966 from: 1 to: 2352
KIBB5634.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 67 2049 2352
Size: 67 1982 303
Fragments arranged by size:
1982 303 67
AccI GT'mk_AC
Cuts at: 0 1310 2352
Size: 1310 1042
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 824 1162 2352
Size: 601 223 338 1190
Fragments arranged by size:
1190 601 338 223
AflIII A'CryG_T
Cuts at: 0 302 2352
Size: 302 2050
AleI CACnn'nnGTG
Cuts at: 0 814 826 1093 2352
Size: 814 12 267 1259
Fragments arranged by size:
1259 814 267 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1918 2352
Size: 1918 434
ApaI G_GGCC'C
Cuts at: 0 2105 2352
Size: 2105 247
ApoI r'AATT_y
Cuts at: 0 1543 2352
Size: 1543 809
AvaI C'yCGr_G
Cuts at: 0 714 2104 2333 2352
Size: 714 1390 229 19
Fragments arranged by size:
1390 714 229 19
BanI G'GyrC_C
Cuts at: 0 34 128 820 1699 2239 2352
Size: 34 94 692 879 540 113
Fragments arranged by size:
879 692 540 113 94 34
BanII G_rGCy'C
Cuts at: 0 835 863 1051 1071 1106 2105 2206 2352
Size: 835 28 188 20 35 999 101 146
Fragments arranged by size:
999 835 188 146 101 35 28 20
BbeI G_GCGC'C
Cuts at: 0 38 132 2352
Size: 38 94 2220
Fragments arranged by size:
2220 94 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 267 812 2352
Size: 267 545 1540
Fragments arranged by size:
1540 545 267
BbvCI CC'TCA_GC
Cuts at: 0 2227 2352
Size: 2227 125
BciVI GTATCCnnnnn_n'
Cuts at: 0 1210 2352
Size: 1210 1142
BclI T'GATC_A
Cuts at: 0 1838 2352
Size: 1838 514
BglI GCCn_nnn'nGGC
Cuts at: 0 2022 2076 2352
Size: 2022 54 276
Fragments arranged by size:
2022 276 54
BglII A'GATC_T
Cuts at: 0 1496 2352
Size: 1496 856
BlpI GC'TnA_GC
Cuts at: 0 2312 2352
Size: 2312 40
Bme1580I G_kGCm'C
Cuts at: 0 721 823 1702 2094 2105 2242 2352
Size: 721 102 879 392 11 137 110
Fragments arranged by size:
879 721 392 137 110 102 11
BmgBI CAC'GTC
Cuts at: 0 303 429 2262 2352
Size: 303 126 1833 90
Fragments arranged by size:
1833 303 126 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 2295 2352
Size: 2295 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 2352
Size: 354 1998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 2352
Size: 412 1940
Bpu10I CC'TnA_GC
Cuts at: 0 2227 2352
Size: 2227 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 545 2352
Size: 545 1807
BsaHI Gr'CG_yC
Cuts at: 0 35 129 2036 2352
Size: 35 94 1907 316
Fragments arranged by size:
1907 316 94 35
BsaWI w'CCGG_w
Cuts at: 0 869 1871 2352
Size: 869 1002 481
Fragments arranged by size:
1002 869 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 248 278 2188 2218 2332 2352
Size: 248 30 1910 30 114 20
Fragments arranged by size:
1910 248 114 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 1954 2352
Size: 10 1944 398
Fragments arranged by size:
1944 398 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2083 2167 2352
Size: 2083 84 185
Fragments arranged by size:
2083 185 84
BsiHKAI G_wGCw'C
Cuts at: 0 1988 2352
Size: 1988 364
BsmI GAATG_Cn'
Cuts at: 0 240 2352
Size: 240 2112
BsmBI CGTCTCn'nnnn_
Cuts at: 0 293 2352
Size: 293 2059
Bsp1286I G_dGCh'C
Cuts at: 0 721 823 835 863 1051 1071 1106 1702
Size: 721 102 12 28 188 20 35 596
Cuts at: 1702 1988 2094 2105 2206 2242 2352
Size: 286 106 11 101 36 110
Fragments arranged by size:
721 596 286 188 110 106 102 101
36 35 28 20 12 11
BspEI T'CCGG_A
Cuts at: 0 1871 2352
Size: 1871 481
BspHI T'CATG_A
Cuts at: 0 11 423 2352
Size: 11 412 1929
Fragments arranged by size:
1929 412 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 254 2049 2352
Size: 67 187 1795 303
Fragments arranged by size:
1795 303 187 67
BsrFI r'CCGG_y
Cuts at: 0 125 2352
Size: 125 2227
BssHII G'CGCG_C
Cuts at: 0 1886 2352
Size: 1886 466
BssSI C'ACGA_G
Cuts at: 0 1915 2352
Size: 1915 437
BstAPI GCAn_nnn'nTGC
Cuts at: 0 79 529 2352
Size: 79 450 1823
Fragments arranged by size:
1823 450 79
BstEII G'GTnAC_C
Cuts at: 0 1926 2352
Size: 1926 426
BstXI CCAn_nnnn'nTGG
Cuts at: 0 732 1347 2352
Size: 732 615 1005
Fragments arranged by size:
1005 732 615
BstYI r'GATC_y
Cuts at: 0 1496 1910 2352
Size: 1496 414 442
Fragments arranged by size:
1496 442 414
BtgI C'CryG_G
Cuts at: 0 1055 1184 1476 1661 1715 2001 2020 2352
Size: 1055 129 292 185 54 286 19 332
Fragments arranged by size:
1055 332 292 286 185 129 54 19
BtsI GCAGTG_nn'
Cuts at: 0 62 71 353 530 1805 2352
Size: 62 9 282 177 1275 547
Fragments arranged by size:
1275 547 282 177 62 9
ClaI AT'CG_AT
Cuts at: 0 605 2352
Size: 605 1747
DrdI GACnn_nn'nnGTC
Cuts at: 0 1306 2352
Size: 1306 1046
EaeI y'GGCC_r
Cuts at: 0 722 1181 1479 1598 1718 1769 2352
Size: 722 459 298 119 120 51 583
Fragments arranged by size:
722 583 459 298 120 119 51
EarI CTCTTCn'nnn_
Cuts at: 0 167 2352
Size: 167 2185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2161 2352
Size: 2161 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 601 824 1162 2352
Size: 412 189 223 338 1190
Fragments arranged by size:
1190 412 338 223 189
EcoO109I rG'GnC_Cy
Cuts at: 0 500 699 849 2101 2290 2352
Size: 500 199 150 1252 189 62
Fragments arranged by size:
1252 500 199 189 150 62
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1668 2352
Size: 1668 684
FspI TGC'GCA
Cuts at: 0 2057 2352
Size: 2057 295
HaeII r_GCGC'y
Cuts at: 0 38 132 2352
Size: 38 94 2220
Fragments arranged by size:
2220 94 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 608 640 1058 1090 1208 1240
Size: 544 32 32 32 418 32 118 32
Cuts at: 1240 1674 1706 2352
Size: 434 32 646
Fragments arranged by size:
646 544 434 418 118 32 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 639 2352
Size: 639 1713
KasI G'GCGC_C
Cuts at: 0 34 128 2352
Size: 34 94 2224
Fragments arranged by size:
2224 94 34
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2276 2352
Size: 2276 76
MscI TGG'CCA
Cuts at: 0 1183 1481 2352
Size: 1183 298 871
Fragments arranged by size:
1183 871 298
MslI CAynn'nnrTG
Cuts at: 0 167 514 814 826 1093 1834 2352
Size: 167 347 300 12 267 741 518
Fragments arranged by size:
741 518 347 300 267 167 12
MspA1I CmG'CkG
Cuts at: 0 1057 1140 1485 1923 2003 2022 2352
Size: 1057 83 345 438 80 19 330
Fragments arranged by size:
1057 438 345 330 83 80 19
NarI GG'CG_CC
Cuts at: 0 35 129 2352
Size: 35 94 2223
Fragments arranged by size:
2223 94 35
NcoI C'CATG_G
Cuts at: 0 1184 1476 2352
Size: 1184 292 876
Fragments arranged by size:
1184 876 292
PasI CC'CwG_GG
Cuts at: 0 696 2235 2352
Size: 696 1539 117
Fragments arranged by size:
1539 696 117
PflMI CCAn_nnn'nTGG
Cuts at: 0 1800 2352
Size: 1800 552
PpuMI rG'GwC_Cy
Cuts at: 0 500 849 2290 2352
Size: 500 349 1441 62
Fragments arranged by size:
1441 500 349 62
PshAI GACnn'nnGTC
Cuts at: 0 505 2352
Size: 505 1847
PspOMI G'GGCC_C
Cuts at: 0 2101 2352
Size: 2101 251
PstI C_TGCA'G
Cuts at: 0 1496 2352
Size: 1496 856
PvuII CAG'CTG
Cuts at: 0 1140 1485 1923 2352
Size: 1140 345 438 429
Fragments arranged by size:
1140 438 429 345
SacII CC_GC'GG
Cuts at: 0 1058 2004 2023 2352
Size: 1058 946 19 329
Fragments arranged by size:
1058 946 329 19
SanDI GG'GwC_CC
Cuts at: 0 2290 2352
Size: 2290 62
SfcI C'TryA_G
Cuts at: 0 1492 2352
Size: 1492 860
SfoI GGC'GCC
Cuts at: 0 36 130 2352
Size: 36 94 2222
Fragments arranged by size:
2222 94 36
SgrAI Cr'CCGG_yG
Cuts at: 0 125 2352
Size: 125 2227
SmaI CCC'GGG
Cuts at: 0 2106 2335 2352
Size: 2106 229 17
Fragments arranged by size:
2106 229 17
StyI C'CwwG_G
Cuts at: 0 1184 1476 1643 2352
Size: 1184 292 167 709
Fragments arranged by size:
1184 709 292 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 136 662 790 2352
Size: 118 18 526 128 1562
Fragments arranged by size:
1562 526 128 118 18
TatI w'GTAC_w
Cuts at: 0 785 881 2352
Size: 785 96 1471
Fragments arranged by size:
1471 785 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 174 206 248 280 2352
Size: 174 32 42 32 2072
Fragments arranged by size:
2072 174 42 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 969 2352
Size: 318 651 1383
Fragments arranged by size:
1383 651 318
XmaI C'CCGG_G
Cuts at: 0 2104 2333 2352
Size: 2104 229 19
Fragments arranged by size:
2104 229 19
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI
DrdI EaeI EarI EciI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI PasI PflMI PpuMI PshAI PspOMI PstI
PvuII SacII SanDI SfcI SfoI SgrAI SmaI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BfrBI BmtI BpuEI BsaAI BsaBI BseYI BsiEI
BsiWI BsrBI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI
CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI HincII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI ScaI SexAI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmnI ZraI