Restriction map for
pF1KB5637
(Linear) (Six-base) MAPSORT of: KIBB5637.seq Check: 4773 from: 1 to: 3231
KIBB5637.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 810 1212 3231
Size: 810 402 2019
Fragments arranged by size:
2019 810 402
AatII G_ACGT'C
Cuts at: 0 207 3231
Size: 207 3024
Acc65I G'GTAC_C
Cuts at: 0 1086 3231
Size: 1086 2145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1400 1493 2660 2976 3231
Size: 854 546 93 1167 316 255
Fragments arranged by size:
1167 854 546 316 255 93
AflIII A'CryG_T
Cuts at: 0 460 3231
Size: 460 2771
AhdI GACnn_n'nnGTC
Cuts at: 0 2367 3231
Size: 2367 864
AleI CACnn'nnGTG
Cuts at: 0 982 3231
Size: 982 2249
AlwNI CAG_nnn'CTG
Cuts at: 0 802 1234 3066 3231
Size: 802 432 1832 165
Fragments arranged by size:
1832 802 432 165
ApaI G_GGCC'C
Cuts at: 0 271 3231
Size: 271 2960
ApoI r'AATT_y
Cuts at: 0 2155 2182 2484 2640 2944 3016 3083 3231
Size: 2155 27 302 156 304 72 67 148
Fragments arranged by size:
2155 304 302 156 148 72 67 27
AseI AT'TA_AT
Cuts at: 0 1774 1787 3043 3231
Size: 1774 13 1256 188
Fragments arranged by size:
1774 1256 188 13
AvaI C'yCGr_G
Cuts at: 0 45 1889 3231
Size: 45 1844 1342
Fragments arranged by size:
1844 1342 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1078 1111 3231
Size: 1078 33 2120
Fragments arranged by size:
2120 1078 33
BamHI G'GATC_C
Cuts at: 0 1413 2737 3231
Size: 1413 1324 494
Fragments arranged by size:
1413 1324 494
BanI G'GyrC_C
Cuts at: 0 1086 1700 3231
Size: 1086 614 1531
Fragments arranged by size:
1531 1086 614
BanII G_rGCy'C
Cuts at: 0 56 271 1454 1661 3212 3231
Size: 56 215 1183 207 1551 19
Fragments arranged by size:
1551 1183 215 207 56 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1273 1560 2308 2453 3231
Size: 1273 287 748 145 778
Fragments arranged by size:
1273 778 748 287 145
BbvCI CC'TCA_GC
Cuts at: 0 1046 3231
Size: 1046 2185
BciVI GTATCCnnnnn_n'
Cuts at: 0 245 819 3231
Size: 245 574 2412
Fragments arranged by size:
2412 574 245
BclI T'GATC_A
Cuts at: 0 2055 3231
Size: 2055 1176
BglI GCCn_nnn'nGGC
Cuts at: 0 1495 3231
Size: 1495 1736
Bme1580I G_kGCm'C
Cuts at: 0 271 3231
Size: 271 2960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 265 365 410 1238 1933 2904 3231
Size: 227 38 100 45 828 695 971 327
Fragments arranged by size:
971 828 695 327 227 100 45 38
Bpu10I CC'TnA_GC
Cuts at: 0 55 554 572 590 608 620 626 686
Size: 55 499 18 18 18 12 6 60
Cuts at: 686 692 704 722 1046 1212 3231
Size: 6 12 18 324 166 2019
Fragments arranged by size:
2019 499 324 166 60 55 18 18
18 18 12 12 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 3231
Size: 99 3132
BsaI GGTCTCn'nnnn_
Cuts at: 0 279 3004 3231
Size: 279 2725 227
Fragments arranged by size:
2725 279 227
BsaAI yAC'GTr
Cuts at: 0 463 2864 3231
Size: 463 2401 367
Fragments arranged by size:
2401 463 367
BsaHI Gr'CG_yC
Cuts at: 0 204 3231
Size: 204 3027
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 482 512 951 981 1005 1035 1972 2002
Size: 482 30 439 30 24 30 937 30
Cuts at: 2002 3231
Size: 1229
Fragments arranged by size:
1229 937 482 439 30 30 30 30
24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 167 230 293 314 317 415 1420
Size: 137 30 63 63 21 3 98 1005
Cuts at: 1420 1423 1462 1477 2770 2907 3145 3231
Size: 3 39 15 1293 137 238 86
Fragments arranged by size:
1293 1005 238 137 137 98 86 63
63 39 30 21 15 3 3
BseYI C'CCAG_C
Cuts at: 0 795 998 2726 3231
Size: 795 203 1728 505
Fragments arranged by size:
1728 795 505 203
BsiEI CG_ry'CG
Cuts at: 0 193 3231
Size: 193 3038
BsiHKAI G_wGCw'C
Cuts at: 0 56 2816 3231
Size: 56 2760 415
Fragments arranged by size:
2760 415 56
BsmI GAATG_Cn'
Cuts at: 0 359 3231
Size: 359 2872
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1393 1820 3210 3231
Size: 1393 427 1390 21
Fragments arranged by size:
1393 1390 427 21
Bsp1286I G_dGCh'C
Cuts at: 0 56 271 1454 1661 2816 3212 3231
Size: 56 215 1183 207 1155 396 19
Fragments arranged by size:
1183 1155 396 215 207 56 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 810 1074 1212 3231
Size: 810 264 138 2019
Fragments arranged by size:
2019 810 264 138
BsrBI CCG'CTC
Cuts at: 0 1563 3231
Size: 1563 1668
BssSI C'ACGA_G
Cuts at: 0 3213 3231
Size: 3213 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1066 3231
Size: 1066 2165
BstBI TT'CG_AA
Cuts at: 0 2299 3231
Size: 2299 932
BstYI r'GATC_y
Cuts at: 0 1413 2737 2895 3031 3231
Size: 1413 1324 158 136 200
Fragments arranged by size:
1413 1324 200 158 136
BtgI C'CryG_G
Cuts at: 0 187 1534 1865 3231
Size: 187 1347 331 1366
Fragments arranged by size:
1366 1347 331 187
BtsI GCAGTG_nn'
Cuts at: 0 119 3231
Size: 119 3112
DrdI GACnn_nn'nnGTC
Cuts at: 0 1719 3231
Size: 1719 1512
EaeI y'GGCC_r
Cuts at: 0 190 2267 3231
Size: 190 2077 964
Fragments arranged by size:
2077 964 190
EagI C'GGCC_G
Cuts at: 0 190 3231
Size: 190 3041
EarI CTCTTCn'nnn_
Cuts at: 0 1340 1376 1418 1454 1816 2657 3231
Size: 1340 36 42 36 362 841 574
Fragments arranged by size:
1340 841 574 362 42 36 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 3231
Size: 19 3212
EcoICRI GAG'CTC
Cuts at: 0 54 3231
Size: 54 3177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 265 365 410 854 1238 1400 1493
Size: 227 38 100 45 444 384 162 93
Cuts at: 1493 1933 2660 2904 2976 3231
Size: 440 727 244 72 255
Fragments arranged by size:
727 444 440 384 255 244 227 162
100 93 72 45 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 242 245 3231
Size: 242 3 2986
Fragments arranged by size:
2986 242 3
EcoO109I rG'GnC_Cy
Cuts at: 0 172 268 2705 3231
Size: 172 96 2437 526
Fragments arranged by size:
2437 526 172 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2189 2785 3231
Size: 2189 596 446
Fragments arranged by size:
2189 596 446
FspI TGC'GCA
Cuts at: 0 878 3231
Size: 878 2353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 129 161 1005 1037 1831 1863 2353 2385
Size: 129 32 844 32 794 32 490 32
Cuts at: 2385 2419 2451 3231
Size: 34 32 780
Fragments arranged by size:
844 794 780 490 129 34 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1641 3231
Size: 1641 1590
HindIII A'AGCT_T
Cuts at: 0 2767 3231
Size: 2767 464
KpnI G_GTAC'C
Cuts at: 0 1090 3231
Size: 1090 2141
MfeI C'AATT_G
Cuts at: 0 2222 3231
Size: 2222 1009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 1735 3231
Size: 747 988 1496
Fragments arranged by size:
1496 988 747
MslI CAynn'nnrTG
Cuts at: 0 982 2862 3231
Size: 982 1880 369
Fragments arranged by size:
1880 982 369
MspA1I CmG'CkG
Cuts at: 0 2272 2726 3231
Size: 2272 454 505
Fragments arranged by size:
2272 505 454
NcoI C'CATG_G
Cuts at: 0 1534 1865 3231
Size: 1534 331 1366
Fragments arranged by size:
1534 1366 331
NdeI CA'TA_TG
Cuts at: 0 3156 3231
Size: 3156 75
NspI r_CATG'y
Cuts at: 0 1119 1944 2200 3231
Size: 1119 825 256 1031
Fragments arranged by size:
1119 1031 825 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 157 3231
Size: 157 3074
PfoI T'CCnGG_A
Cuts at: 0 243 3231
Size: 243 2988
PmlI CAC'GTG
Cuts at: 0 463 2864 3231
Size: 463 2401 367
Fragments arranged by size:
2401 463 367
PpuMI rG'GwC_Cy
Cuts at: 0 2705 3231
Size: 2705 526
PsiI TTA'TAA
Cuts at: 0 449 1280 1577 3022 3231
Size: 449 831 297 1445 209
Fragments arranged by size:
1445 831 449 297 209
PspOMI G'GGCC_C
Cuts at: 0 267 3231
Size: 267 2964
PstI C_TGCA'G
Cuts at: 0 807 3231
Size: 807 2424
SacI G_AGCT'C
Cuts at: 0 56 3231
Size: 56 3175
SapI GCTCTTCn'nnn_
Cuts at: 0 1376 1418 2657 3231
Size: 1376 42 1239 574
Fragments arranged by size:
1376 1239 574 42
ScaI AGT'ACT
Cuts at: 0 3113 3231
Size: 3113 118
SfcI C'TryA_G
Cuts at: 0 803 944 971 3231
Size: 803 141 27 2260
Fragments arranged by size:
2260 803 141 27
SmaI CCC'GGG
Cuts at: 0 47 3231
Size: 47 3184
SmlI C'TyrA_G
Cuts at: 0 78 3231
Size: 78 3153
SphI G_CATG'C
Cuts at: 0 1119 2200 3231
Size: 1119 1081 1031
Fragments arranged by size:
1119 1081 1031
StuI AGG'CCT
Cuts at: 0 76 3231
Size: 76 3155
StyI C'CwwG_G
Cuts at: 0 1534 1865 2120 3231
Size: 1534 331 255 1111
Fragments arranged by size:
1534 1111 331 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 909 1622 1718 2255 3231
Size: 909 713 96 537 976
Fragments arranged by size:
976 909 713 537 96
TatI w'GTAC_w
Cuts at: 0 2462 3111 3231
Size: 2462 649 120
Fragments arranged by size:
2462 649 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1908 3231
Size: 1908 1323
XmaI C'CCGG_G
Cuts at: 0 45 3231
Size: 45 3186
XmnI GAAnn'nnTTC
Cuts at: 0 1930 3231
Size: 1930 1301
ZraI GAC'GTC
Cuts at: 0 205 3231
Size: 205 3026
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AleI AlwNI
ApaI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BciVI BclI BglI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BssSI BstAPI
BstBI BstYI BtgI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI Hin4I
HincII HindIII KpnI MfeI MmeI MslI MspA1I NcoI
NdeI NspI PflMI PfoI PmlI PpuMI PsiI PspOMI
PstI SacI SapI ScaI SfcI SmaI SmlI SphI
StuI StyI TaqII TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AlfI AloI ApaLI
AscI AsiSI AvrII BbeI BcgI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaBI BsaWI BsgI BsiWI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRI
EcoRV FseI FspAI HaeII HpaI KasI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PshAI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XhoI