(Linear) (Six-base) MAPSORT of: KIBB5638.seq  Check: 8429  from: 1  to: 564



KIBB5638.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AccI GT'mk_AC

Cuts at:      0     374     564 
   Size:        374     190
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     564 
   Size:        338     226
 
ApoI r'AATT_y

Cuts at:      0     413     485     564 
   Size:        413      72      79

  Fragments arranged by size: 

                 413      79      72
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     230     564 
   Size:        230     334
 
Bpu10I CC'TnA_GC

Cuts at:      0     389     564 
   Size:        389     175
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     199     564 
   Size:        199     365
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     166     564 
   Size:        166     398
 
BsrDI GCAATG_nn'

Cuts at:      0     203     564 
   Size:        203     361
 
BstBI TT'CG_AA

Cuts at:      0     489     564 
   Size:        489      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     564 
   Size:        338     226
 
EcoRI G'AATT_C

Cuts at:      0     413     564 
   Size:        413     151
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      69     564 
   Size:         69     495
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     124     564 
   Size:          6     118     440

  Fragments arranged by size: 

                 440     118       6
 
SfcI C'TryA_G

Cuts at:      0     117     564 
   Size:        117     447
 
TatI w'GTAC_w

Cuts at:      0     188     209     564 
   Size:        188      21     355

  Fragments arranged by size: 

                 355     188      21
 
XbaI T'CTAG_A

Cuts at:      0     364     564 
   Size:        364     200
 
XmnI GAAnn'nnTTC

Cuts at:      0     275     564 
   Size:        275     289
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BmrI   Bpu10I    BseRI    BspMI    BsrDI
    BstBI  Eco57MI    EcoRI     FalI     MmeI     SfcI     TatI     XbaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI