Restriction map for
pF1KB5639
(Linear) (Six-base) MAPSORT of: KIBB5639.seq Check: 5758 from: 1 to: 1320
KIBB5639.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AflIII A'CryG_T
Cuts at: 0 902 1320
Size: 902 418
AlwNI CAG_nnn'CTG
Cuts at: 0 949 1309 1320
Size: 949 360 11
Fragments arranged by size:
949 360 11
ApaI G_GGCC'C
Cuts at: 0 42 1320
Size: 42 1278
AvaI C'yCGr_G
Cuts at: 0 913 1320
Size: 913 407
BanI G'GyrC_C
Cuts at: 0 424 1044 1320
Size: 424 620 276
Fragments arranged by size:
620 424 276
BanII G_rGCy'C
Cuts at: 0 42 95 1273 1320
Size: 42 53 1178 47
Fragments arranged by size:
1178 53 47 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 272 1320
Size: 272 1048
BbvCI CC'TCA_GC
Cuts at: 0 800 1118 1320
Size: 800 318 202
Fragments arranged by size:
800 318 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1015 1049 1320
Size: 1015 34 271
Fragments arranged by size:
1015 271 34
BclI T'GATC_A
Cuts at: 0 164 1320
Size: 164 1156
Bme1580I G_kGCm'C
Cuts at: 0 42 1049 1320
Size: 42 1007 271
Fragments arranged by size:
1007 271 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 582 1320
Size: 537 45 738
Fragments arranged by size:
738 537 45
Bpu10I CC'TnA_GC
Cuts at: 0 744 764 800 854 1118 1320
Size: 744 20 36 54 264 202
Fragments arranged by size:
744 264 202 54 36 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 150 962 1320
Size: 150 812 358
Fragments arranged by size:
812 358 150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 641 671 1320
Size: 641 30 649
Fragments arranged by size:
649 641 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 978 981 1320
Size: 598 380 3 339
Fragments arranged by size:
598 380 339 3
BseYI C'CCAG_C
Cuts at: 0 188 573 1224 1320
Size: 188 385 651 96
Fragments arranged by size:
651 385 188 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 369 1320
Size: 330 39 951
Fragments arranged by size:
951 330 39
BsiEI CG_ry'CG
Cuts at: 0 29 1320
Size: 29 1291
BsiHKAI G_wGCw'C
Cuts at: 0 119 1273 1320
Size: 119 1154 47
Fragments arranged by size:
1154 119 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 580 1213 1320
Size: 580 633 107
Fragments arranged by size:
633 580 107
Bsp1286I G_dGCh'C
Cuts at: 0 42 95 119 1049 1273 1320
Size: 42 53 24 930 224 47
Fragments arranged by size:
930 224 53 47 42 24
BspHI T'CATG_A
Cuts at: 0 1070 1320
Size: 1070 250
BsrBI CCG'CTC
Cuts at: 0 199 1068 1320
Size: 199 869 252
Fragments arranged by size:
869 252 199
BsrFI r'CCGG_y
Cuts at: 0 939 1320
Size: 939 381
BsrGI T'GTAC_A
Cuts at: 0 508 1145 1320
Size: 508 637 175
Fragments arranged by size:
637 508 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 287 1309 1320
Size: 287 1022 11
Fragments arranged by size:
1022 287 11
BstEII G'GTnAC_C
Cuts at: 0 591 1320
Size: 591 729
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 1320
Size: 489 831
BtgI C'CryG_G
Cuts at: 0 128 1320
Size: 128 1192
DraIII CAC_nnn'GTG
Cuts at: 0 353 407 476 1320
Size: 353 54 69 844
Fragments arranged by size:
844 353 69 54
EaeI y'GGCC_r
Cuts at: 0 26 1320
Size: 26 1294
EagI C'GGCC_G
Cuts at: 0 26 1320
Size: 26 1294
EcoICRI GAG'CTC
Cuts at: 0 1271 1320
Size: 1271 49
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 582 1320
Size: 537 45 738
Fragments arranged by size:
738 537 45
EcoO109I rG'GnC_Cy
Cuts at: 0 38 61 99 759 849 1320
Size: 38 23 38 660 90 471
Fragments arranged by size:
660 471 90 38 38 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 242 266 268 274 298 300 476 508
Size: 242 24 2 6 24 2 176 32
Cuts at: 508 766 798 1320
Size: 258 32 522
Fragments arranged by size:
522 258 242 176 32 32 24 24
6 2 2
HincII GTy'rAC
Cuts at: 0 900 1170 1320
Size: 900 270 150
Fragments arranged by size:
900 270 150
MslI CAynn'nnrTG
Cuts at: 0 320 1320
Size: 320 1000
MspA1I CmG'CkG
Cuts at: 0 130 151 577 1126 1320
Size: 130 21 426 549 194
Fragments arranged by size:
549 426 194 130 21
NspI r_CATG'y
Cuts at: 0 906 1320
Size: 906 414
PasI CC'CwG_GG
Cuts at: 0 65 604 1320
Size: 65 539 716
Fragments arranged by size:
716 539 65
PciI A'CATG_T
Cuts at: 0 902 1320
Size: 902 418
PfoI T'CCnGG_A
Cuts at: 0 431 872 1320
Size: 431 441 448
Fragments arranged by size:
448 441 431
PpuMI rG'GwC_Cy
Cuts at: 0 61 99 759 849 1320
Size: 61 38 660 90 471
Fragments arranged by size:
660 471 90 61 38
PspOMI G'GGCC_C
Cuts at: 0 38 1320
Size: 38 1282
PvuII CAG'CTG
Cuts at: 0 151 577 1320
Size: 151 426 743
Fragments arranged by size:
743 426 151
SacI G_AGCT'C
Cuts at: 0 1273 1320
Size: 1273 47
SacII CC_GC'GG
Cuts at: 0 131 1320
Size: 131 1189
SanDI GG'GwC_CC
Cuts at: 0 99 759 1320
Size: 99 660 561
Fragments arranged by size:
660 561 99
SexAI A'CCwGG_T
Cuts at: 0 349 1320
Size: 349 971
SfcI C'TryA_G
Cuts at: 0 306 1320
Size: 306 1014
SmaI CCC'GGG
Cuts at: 0 915 1320
Size: 915 405
StuI AGG'CCT
Cuts at: 0 87 1320
Size: 87 1233
StyI C'CwwG_G
Cuts at: 0 1214 1320
Size: 1214 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 184 859 1320
Size: 184 675 461
Fragments arranged by size:
675 461 184
TatI w'GTAC_w
Cuts at: 0 508 1145 1320
Size: 508 637 175
Fragments arranged by size:
637 508 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 462 494 1320
Size: 462 32 826
Fragments arranged by size:
826 462 32
Tth111I GACn'n_nGTC
Cuts at: 0 808 1320
Size: 808 512
XbaI T'CTAG_A
Cuts at: 0 1290 1320
Size: 1290 30
XmaI C'CCGG_G
Cuts at: 0 913 1320
Size: 913 407
Enzymes that do cut:
AflIII AlwNI ApaI AvaI BanI BanII BbsI BbvCI
BcgI BclI Bme1580I BpmI Bpu10I BsaI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI
BsrFI BsrGI BstAPI BstEII BstXI BtgI DraIII EaeI
EagI EcoICRI Eco57MI EcoO109I Hin4I HincII MslI MspA1I
NspI PasI PciI PfoI PpuMI PspOMI PvuII SacI
SacII SanDI SexAI SfcI SmaI StuI StyI TaqII
TatI TstI Tth111I XbaI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BspEI BspMI
BsrDI BssHII BssSI BstBI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DrdI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XcmI XhoI XmnI ZraI