(Linear) (Six-base) MAPSORT of: KIBB5643.seq  Check: 2585  from: 1  to: 216



KIBB5643.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     216 
   Size:        158      58
 
AhdI GACnn_n'nnGTC

Cuts at:      0     117     216 
   Size:        117      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     216 
   Size:         73     143
 
BstBI TT'CG_AA

Cuts at:      0      97     216 
   Size:         97     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     216 
   Size:        158      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     216 
   Size:        103      32      81

  Fragments arranged by size: 

                 103      81      32
 
MspA1I CmG'CkG

Cuts at:      0     109     216 
   Size:        109     107
 
NspI r_CATG'y

Cuts at:      0     139     216 
   Size:        139      77
 

 Enzymes that do cut:

     AcuI     AhdI     BsgI    BstBI  Eco57MI    Hin4I   MspA1I     NspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI