Restriction map for
pF1KB5645
(Linear) (Six-base) MAPSORT of: KIBB5645.seq Check: 9469 from: 1 to: 3510
KIBB5645.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3233 3510
Size: 3233 277
AatII G_ACGT'C
Cuts at: 0 542 3510
Size: 542 2968
AccI GT'mk_AC
Cuts at: 0 2447 3438 3510
Size: 2447 991 72
Fragments arranged by size:
2447 991 72
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 926 3006 3510
Size: 333 593 2080 504
Fragments arranged by size:
2080 593 504 333
AflIII A'CryG_T
Cuts at: 0 1348 3510
Size: 1348 2162
AgeI A'CCGG_T
Cuts at: 0 1817 3510
Size: 1817 1693
AhdI GACnn_n'nnGTC
Cuts at: 0 22 3510
Size: 22 3488
AleI CACnn'nnGTG
Cuts at: 0 868 1820 3510
Size: 868 952 1690
Fragments arranged by size:
1690 952 868
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1798 2752 3510
Size: 1798 954 758
Fragments arranged by size:
1798 954 758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1155 1187 3510
Size: 1155 32 2323
Fragments arranged by size:
2323 1155 32
AlwNI CAG_nnn'CTG
Cuts at: 0 70 1090 3214 3510
Size: 70 1020 2124 296
Fragments arranged by size:
2124 1020 296 70
ApaI G_GGCC'C
Cuts at: 0 154 495 3510
Size: 154 341 3015
Fragments arranged by size:
3015 341 154
ApaLI G'TGCA_C
Cuts at: 0 1755 3510
Size: 1755 1755
ApoI r'AATT_y
Cuts at: 0 1193 2237 3510
Size: 1193 1044 1273
Fragments arranged by size:
1273 1193 1044
AvaI C'yCGr_G
Cuts at: 0 299 3174 3510
Size: 299 2875 336
Fragments arranged by size:
2875 336 299
BamHI G'GATC_C
Cuts at: 0 1373 3510
Size: 1373 2137
BanI G'GyrC_C
Cuts at: 0 49 134 376 3028 3510
Size: 49 85 242 2652 482
Fragments arranged by size:
2652 482 242 85 49
BanII G_rGCy'C
Cuts at: 0 154 495 1246 3165 3510
Size: 154 341 751 1919 345
Fragments arranged by size:
1919 751 345 341 154
BbeI G_GCGC'C
Cuts at: 0 138 3510
Size: 138 3372
BbsI GAAGACnn'nnnn_
Cuts at: 0 301 339 491 549 677 2186 3447 3510
Size: 301 38 152 58 128 1509 1261 63
Fragments arranged by size:
1509 1261 301 152 128 63 58 38
BbvCI CC'TCA_GC
Cuts at: 0 388 934 3510
Size: 388 546 2576
Fragments arranged by size:
2576 546 388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3046 3080 3271 3305 3510
Size: 3046 34 191 34 205
Fragments arranged by size:
3046 205 191 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2425 3163 3510
Size: 2425 738 347
Fragments arranged by size:
2425 738 347
BclI T'GATC_A
Cuts at: 0 1364 3510
Size: 1364 2146
BfrBI ATG'CAT
Cuts at: 0 39 3510
Size: 39 3471
BglI GCCn_nnn'nGGC
Cuts at: 0 2644 3510
Size: 2644 866
BglII A'GATC_T
Cuts at: 0 1616 3510
Size: 1616 1894
Bme1580I G_kGCm'C
Cuts at: 0 154 307 379 495 1759 2633 2741 3510
Size: 154 153 72 116 1264 874 108 769
Fragments arranged by size:
1264 874 769 154 153 116 108 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 1566 2090 2937 3126 3150 3510
Size: 1566 524 847 189 24 360
Fragments arranged by size:
1566 847 524 360 189 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 959 1182 1263 3510
Size: 959 223 81 2247
Fragments arranged by size:
2247 959 223 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 681 1332 3510
Size: 340 341 651 2178
Fragments arranged by size:
2178 651 341 340
Bpu10I CC'TnA_GC
Cuts at: 0 388 934 3164 3510
Size: 388 546 2230 346
Fragments arranged by size:
2230 546 388 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3446 3510
Size: 3446 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 560 1151 1432 2306 2854 3510
Size: 560 591 281 874 548 656
Fragments arranged by size:
874 656 591 560 548 281
BsaAI yAC'GTr
Cuts at: 0 417 3510
Size: 417 3093
BsaHI Gr'CG_yC
Cuts at: 0 23 135 539 608 1460 2384 3330 3510
Size: 23 112 404 69 852 924 946 180
Fragments arranged by size:
946 924 852 404 180 112 69 23
BsaWI w'CCGG_w
Cuts at: 0 1817 2523 3510
Size: 1817 706 987
Fragments arranged by size:
1817 987 706
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1517 1547 3510
Size: 1517 30 1963
Fragments arranged by size:
1963 1517 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 1236 1541 3510
Size: 706 530 305 1969
Fragments arranged by size:
1969 706 530 305
BseYI C'CCAG_C
Cuts at: 0 168 494 1120 1393 1481 2277 3510
Size: 168 326 626 273 88 796 1233
Fragments arranged by size:
1233 796 626 326 273 168 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 990 1932 3510
Size: 499 491 942 1578
Fragments arranged by size:
1578 942 499 491
BsiEI CG_ry'CG
Cuts at: 0 1209 3510
Size: 1209 2301
BsiHKAI G_wGCw'C
Cuts at: 0 1759 1817 3224 3510
Size: 1759 58 1407 286
Fragments arranged by size:
1759 1407 286 58
BsmI GAATG_Cn'
Cuts at: 0 59 531 1105 2705 3510
Size: 59 472 574 1600 805
Fragments arranged by size:
1600 805 574 472 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1555 2375 2402 3337 3510
Size: 1555 820 27 935 173
Fragments arranged by size:
1555 935 820 173 27
Bsp1286I G_dGCh'C
Cuts at: 0 154 307 379 495 1246 1759 1817 2633
Size: 154 153 72 116 751 513 58 816
Cuts at: 2633 2741 3165 3224 3510
Size: 108 424 59 286
Fragments arranged by size:
816 751 513 424 286 154 153 116
108 72 59 58
BspEI T'CCGG_A
Cuts at: 0 2523 3510
Size: 2523 987
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1273 3233 3510
Size: 1273 1960 277
Fragments arranged by size:
1960 1273 277
BsrBI CCG'CTC
Cuts at: 0 324 1211 3510
Size: 324 887 2299
Fragments arranged by size:
2299 887 324
BsrDI GCAATG_nn'
Cuts at: 0 1194 2058 3510
Size: 1194 864 1452
Fragments arranged by size:
1452 1194 864
BsrFI r'CCGG_y
Cuts at: 0 448 1672 1817 1873 3510
Size: 448 1224 145 56 1637
Fragments arranged by size:
1637 1224 448 145 56
BsrGI T'GTAC_A
Cuts at: 0 500 3510
Size: 500 3010
BssSI C'ACGA_G
Cuts at: 0 2734 3156 3510
Size: 2734 422 354
Fragments arranged by size:
2734 422 354
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1589 1754 2063 3510
Size: 1589 165 309 1447
Fragments arranged by size:
1589 1447 309 165
BstEII G'GTnAC_C
Cuts at: 0 1493 3510
Size: 1493 2017
BstXI CCAn_nnnn'nTGG
Cuts at: 0 570 3510
Size: 570 2940
BstYI r'GATC_y
Cuts at: 0 1373 1616 3504 3510
Size: 1373 243 1888 6
Fragments arranged by size:
1888 1373 243 6
Bsu36I CC'TnA_GG
Cuts at: 0 280 679 3510
Size: 280 399 2831
Fragments arranged by size:
2831 399 280
BtgI C'CryG_G
Cuts at: 0 1150 1327 1498 1910 3510
Size: 1150 177 171 412 1600
Fragments arranged by size:
1600 1150 412 177 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 564 579 2783 3510
Size: 564 15 2204 727
Fragments arranged by size:
2204 727 564 15
BtsI GCAGTG_nn'
Cuts at: 0 2133 3510
Size: 2133 1377
ClaI AT'CG_AT
Cuts at: 0 2825 3510
Size: 2825 685
DraI TTT'AAA
Cuts at: 0 1296 3510
Size: 1296 2214
DraIII CAC_nnn'GTG
Cuts at: 0 420 1013 3278 3510
Size: 420 593 2265 232
Fragments arranged by size:
2265 593 420 232
EaeI y'GGCC_r
Cuts at: 0 339 450 572 777 923 1206 1890 1913
Size: 339 111 122 205 146 283 684 23
Cuts at: 1913 2117 2595 3261 3510
Size: 204 478 666 249
Fragments arranged by size:
684 666 478 339 283 249 205 204
146 122 111 23
EagI C'GGCC_G
Cuts at: 0 1206 3510
Size: 1206 2304
EarI CTCTTCn'nnn_
Cuts at: 0 454 902 3510
Size: 454 448 2608
Fragments arranged by size:
2608 454 448
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 89 2860 3510
Size: 89 2771 650
Fragments arranged by size:
2771 650 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 340 681 926 1332 3006 3510
Size: 333 7 341 245 406 1674 504
Fragments arranged by size:
1674 504 406 341 333 245 7
EcoO109I rG'GnC_Cy
Cuts at: 0 150 151 491 855 1486 1958 3211 3406
Size: 150 1 340 364 631 472 1253 195
Cuts at: 3406 3510
Size: 104
Fragments arranged by size:
1253 631 472 364 340 195 150 104
1
EcoRI G'AATT_C
Cuts at: 0 1193 3510
Size: 1193 2317
FspI TGC'GCA
Cuts at: 0 862 3271 3510
Size: 862 2409 239
Fragments arranged by size:
2409 862 239
FspAI rTGC'GCAy
Cuts at: 0 3271 3510
Size: 3271 239
HaeII r_GCGC'y
Cuts at: 0 138 3510
Size: 138 3372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 578 610 814 846 1129 1161
Size: 8 32 538 32 204 32 283 32
Cuts at: 1161 2399 2431 2527 2559 2874 2906 3317 3349
Size: 1238 32 96 32 315 32 411 32
Cuts at: 3349 3510
Size: 161
Fragments arranged by size:
1238 538 411 315 283 204 161 96
32 32 32 32 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 2850 2970 3510
Size: 2850 120 540
Fragments arranged by size:
2850 540 120
KasI G'GCGC_C
Cuts at: 0 134 3510
Size: 134 3376
MfeI C'AATT_G
Cuts at: 0 1640 2502 3510
Size: 1640 862 1008
Fragments arranged by size:
1640 1008 862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 515 2466 3510
Size: 515 1951 1044
Fragments arranged by size:
1951 1044 515
MscI TGG'CCA
Cuts at: 0 341 574 779 925 2119 2597 3263 3510
Size: 341 233 205 146 1194 478 666 247
Fragments arranged by size:
1194 666 478 341 247 233 205 146
MslI CAynn'nnrTG
Cuts at: 0 44 868 1820 2473 2690 2810 3364 3510
Size: 44 824 952 653 217 120 554 146
Fragments arranged by size:
952 824 653 554 217 146 120 44
MspA1I CmG'CkG
Cuts at: 0 498 1706 2024 2318 2532 3510
Size: 498 1208 318 294 214 978
Fragments arranged by size:
1208 978 498 318 294 214
NaeI GCC'GGC
Cuts at: 0 1674 3510
Size: 1674 1836
NarI GG'CG_CC
Cuts at: 0 135 3510
Size: 135 3375
NcoI C'CATG_G
Cuts at: 0 1150 1327 1498 3510
Size: 1150 177 171 2012
Fragments arranged by size:
2012 1150 177 171
NgoMIV G'CCGG_C
Cuts at: 0 1672 3510
Size: 1672 1838
NotI GC'GGCC_GC
Cuts at: 0 1206 3510
Size: 1206 2304
NsiI A_TGCA'T
Cuts at: 0 41 3510
Size: 41 3469
NspI r_CATG'y
Cuts at: 0 43 1352 1909 3510
Size: 43 1309 557 1601
Fragments arranged by size:
1601 1309 557 43
PciI A'CATG_T
Cuts at: 0 1348 3510
Size: 1348 2162
PflMI CCAn_nnn'nTGG
Cuts at: 0 70 231 1645 3510
Size: 70 161 1414 1865
Fragments arranged by size:
1865 1414 161 70
PfoI T'CCnGG_A
Cuts at: 0 842 2979 3510
Size: 842 2137 531
Fragments arranged by size:
2137 842 531
PmlI CAC'GTG
Cuts at: 0 417 3510
Size: 417 3093
PpuMI rG'GwC_Cy
Cuts at: 0 1486 1958 3406 3510
Size: 1486 472 1448 104
Fragments arranged by size:
1486 1448 472 104
PshAI GACnn'nnGTC
Cuts at: 0 2258 2327 2618 2834 3290 3510
Size: 2258 69 291 216 456 220
Fragments arranged by size:
2258 456 291 220 216 69
PspOMI G'GGCC_C
Cuts at: 0 150 491 3510
Size: 150 341 3019
Fragments arranged by size:
3019 341 150
PstI C_TGCA'G
Cuts at: 0 638 884 2397 2731 3510
Size: 638 246 1513 334 779
Fragments arranged by size:
1513 779 638 334 246
PvuII CAG'CTG
Cuts at: 0 498 1706 2532 3510
Size: 498 1208 826 978
Fragments arranged by size:
1208 978 826 498
SanDI GG'GwC_CC
Cuts at: 0 1958 3510
Size: 1958 1552
SapI GCTCTTCn'nnn_
Cuts at: 0 454 3510
Size: 454 3056
SexAI A'CCwGG_T
Cuts at: 0 230 2105 3510
Size: 230 1875 1405
Fragments arranged by size:
1875 1405 230
SfcI C'TryA_G
Cuts at: 0 634 880 2004 2393 2727 2990 3321 3510
Size: 634 246 1124 389 334 263 331 189
Fragments arranged by size:
1124 634 389 334 331 263 246 189
SfoI GGC'GCC
Cuts at: 0 136 3510
Size: 136 3374
SgrAI Cr'CCGG_yG
Cuts at: 0 1817 3510
Size: 1817 1693
SmaI CCC'GGG
Cuts at: 0 301 3510
Size: 301 3209
SmlI C'TyrA_G
Cuts at: 0 3461 3510
Size: 3461 49
StyI C'CwwG_G
Cuts at: 0 402 1150 1327 1489 1498 2510 3510
Size: 402 748 177 162 9 1012 1000
Fragments arranged by size:
1012 1000 748 402 177 162 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1239 2878 3145 3510
Size: 1239 1639 267 365
Fragments arranged by size:
1639 1239 365 267
TatI w'GTAC_w
Cuts at: 0 500 971 1684 3510
Size: 500 471 713 1826
Fragments arranged by size:
1826 713 500 471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1404 1436 1701 1733 3510
Size: 1404 32 265 32 1777
Fragments arranged by size:
1777 1404 265 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 1157 2103 2659 3510
Size: 840 317 946 556 851
Fragments arranged by size:
946 851 840 556 317
XmaI C'CCGG_G
Cuts at: 0 299 3510
Size: 299 3211
XmnI GAAnn'nnTTC
Cuts at: 0 3310 3510
Size: 3310 200
ZraI GAC'GTC
Cuts at: 0 540 3510
Size: 540 2970
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NsiI
NspI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII SanDI SapI SexAI SfcI SfoI SgrAI
SmaI SmlI StyI TaqII TatI TstI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AscI AseI AsiSI AvrII
BaeI BlpI BmgBI BmtI BsaBI BsiWI BspHI BssHII
BstBI BstZ17I CspCI DrdI EcoICRI EcoNI EcoRV FalI
FseI HindIII HpaI KpnI MluI NdeI NheI NruI
PacI PasI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI