Restriction map for
pF1KB5648
(Linear) (Six-base) MAPSORT of: KIBB5648.seq Check: 7970 from: 1 to: 2478
KIBB5648.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 732 2340 2478
Size: 732 1608 138
Fragments arranged by size:
1608 732 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1082 1643 2478
Size: 122 960 561 835
Fragments arranged by size:
960 835 561 122
AflII C'TTAA_G
Cuts at: 0 1168 2478
Size: 1168 1310
AflIII A'CryG_T
Cuts at: 0 2066 2259 2478
Size: 2066 193 219
Fragments arranged by size:
2066 219 193
AgeI A'CCGG_T
Cuts at: 0 1086 2478
Size: 1086 1392
AhdI GACnn_n'nnGTC
Cuts at: 0 1496 2478
Size: 1496 982
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2085 2117 2478
Size: 2085 32 361
Fragments arranged by size:
2085 361 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1210 1258 2104 2478
Size: 1210 48 846 374
Fragments arranged by size:
1210 846 374 48
ApoI r'AATT_y
Cuts at: 0 701 751 984 1338 1588 2478
Size: 701 50 233 354 250 890
Fragments arranged by size:
890 701 354 250 233 50
AvaI C'yCGr_G
Cuts at: 0 65 2478
Size: 65 2413
BanI G'GyrC_C
Cuts at: 0 7 2478
Size: 7 2471
BbeI G_GCGC'C
Cuts at: 0 11 2478
Size: 11 2467
BbsI GAAGACnn'nnnn_
Cuts at: 0 1631 1904 2478
Size: 1631 273 574
Fragments arranged by size:
1631 574 273
BclI T'GATC_A
Cuts at: 0 852 2478
Size: 852 1626
BglII A'GATC_T
Cuts at: 0 79 2478
Size: 79 2399
BmrI ACTGGGnnnn_n'
Cuts at: 0 1541 2478
Size: 1541 937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 2478
Size: 1232 1246
Bpu10I CC'TnA_GC
Cuts at: 0 1532 2478
Size: 1532 946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1294 2478
Size: 941 353 1184
Fragments arranged by size:
1184 941 353
BsaAI yAC'GTr
Cuts at: 0 597 2478
Size: 597 1881
BsaBI GATnn'nnATC
Cuts at: 0 2274 2478
Size: 2274 204
BsaHI Gr'CG_yC
Cuts at: 0 8 2478
Size: 8 2470
BsaWI w'CCGG_w
Cuts at: 0 1086 2478
Size: 1086 1392
BseYI C'CCAG_C
Cuts at: 0 1203 2346 2478
Size: 1203 1143 132
Fragments arranged by size:
1203 1143 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1388 2478
Size: 564 824 1090
Fragments arranged by size:
1090 824 564
BsmI GAATG_Cn'
Cuts at: 0 2148 2478
Size: 2148 330
BspHI T'CATG_A
Cuts at: 0 873 2478
Size: 873 1605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 661 2478
Size: 661 1817
BsrBI CCG'CTC
Cuts at: 0 818 2478
Size: 818 1660
BsrDI GCAATG_nn'
Cuts at: 0 1352 2478
Size: 1352 1126
BsrFI r'CCGG_y
Cuts at: 0 10 1086 2478
Size: 10 1076 1392
Fragments arranged by size:
1392 1076 10
BstBI TT'CG_AA
Cuts at: 0 1386 2478
Size: 1386 1092
BstYI r'GATC_y
Cuts at: 0 79 2478
Size: 79 2399
Bsu36I CC'TnA_GG
Cuts at: 0 2105 2478
Size: 2105 373
BtsI GCAGTG_nn'
Cuts at: 0 576 1850 2478
Size: 576 1274 628
Fragments arranged by size:
1274 628 576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 915 950 2478
Size: 915 35 1528
Fragments arranged by size:
1528 915 35
DraIII CAC_nnn'GTG
Cuts at: 0 393 2478
Size: 393 2085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1082 1232 1643 2478
Size: 122 960 150 411 835
Fragments arranged by size:
960 835 411 150 122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2417 2437 2478
Size: 2417 20 41
Fragments arranged by size:
2417 41 20
EcoRI G'AATT_C
Cuts at: 0 984 1588 2478
Size: 984 604 890
Fragments arranged by size:
984 890 604
HaeII r_GCGC'y
Cuts at: 0 11 2478
Size: 11 2467
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 154 173 186 852 884 1205 1237
Size: 141 13 19 13 666 32 321 32
Cuts at: 1237 1470 1482 1502 1514 1671 1703 1723 1755
Size: 233 12 20 12 157 32 20 32
Cuts at: 1755 1905 1937 2478
Size: 150 32 541
Fragments arranged by size:
666 541 321 233 157 150 141 32
32 32 32 32 20 20 19 13
13 12 12
HincII GTy'rAC
Cuts at: 0 733 2475 2478
Size: 733 1742 3
Fragments arranged by size:
1742 733 3
HindIII A'AGCT_T
Cuts at: 0 495 2478
Size: 495 1983
HpaI GTT'AAC
Cuts at: 0 2475 2478
Size: 2475 3
KasI G'GCGC_C
Cuts at: 0 7 2478
Size: 7 2471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1577 1972 2478
Size: 1577 395 506
Fragments arranged by size:
1577 506 395
MslI CAynn'nnrTG
Cuts at: 0 413 452 599 2478
Size: 413 39 147 1879
Fragments arranged by size:
1879 413 147 39
MspA1I CmG'CkG
Cuts at: 0 1210 1366 2382 2478
Size: 1210 156 1016 96
Fragments arranged by size:
1210 1016 156 96
NaeI GCC'GGC
Cuts at: 0 12 2478
Size: 12 2466
NarI GG'CG_CC
Cuts at: 0 8 2478
Size: 8 2470
NgoMIV G'CCGG_C
Cuts at: 0 10 2478
Size: 10 2468
NspI r_CATG'y
Cuts at: 0 2339 2478
Size: 2339 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 1210 2478
Size: 1210 1268
PstI C_TGCA'G
Cuts at: 0 2441 2478
Size: 2441 37
PvuII CAG'CTG
Cuts at: 0 2382 2478
Size: 2382 96
SalI G'TCGA_C
Cuts at: 0 731 2478
Size: 731 1747
SfcI C'TryA_G
Cuts at: 0 2096 2437 2478
Size: 2096 341 41
Fragments arranged by size:
2096 341 41
SfoI GGC'GCC
Cuts at: 0 9 2478
Size: 9 2469
SgrAI Cr'CCGG_yG
Cuts at: 0 10 2478
Size: 10 2468
SmlI C'TyrA_G
Cuts at: 0 65 956 1168 1273 2478
Size: 65 891 212 105 1205
Fragments arranged by size:
1205 891 212 105 65
SpeI A'CTAG_T
Cuts at: 0 1954 2478
Size: 1954 524
SspI AAT'ATT
Cuts at: 0 696 832 1555 1910 2478
Size: 696 136 723 355 568
Fragments arranged by size:
723 696 568 355 136
StuI AGG'CCT
Cuts at: 0 169 2478
Size: 169 2309
StyI C'CwwG_G
Cuts at: 0 284 2478
Size: 284 2194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 701 1329 2478
Size: 701 628 1149
Fragments arranged by size:
1149 701 628
TatI w'GTAC_w
Cuts at: 0 911 2478
Size: 911 1567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 377 409 2478
Size: 377 32 2069
Fragments arranged by size:
2069 377 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 625 2478
Size: 625 1853
XhoI C'TCGA_G
Cuts at: 0 65 2478
Size: 65 2413
XmnI GAAnn'nnTTC
Cuts at: 0 677 2478
Size: 677 1801
Enzymes that do cut:
AccI AcuI AflII AflIII AgeI AhdI AloI AlwNI
ApoI AvaI BanI BbeI BbsI BclI BglII BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsmI BspHI BspMI BsrBI BsrDI BsrFI BstBI
BstYI Bsu36I BtsI CspCI DraIII Eco57MI EcoNI EcoRI
HaeII Hin4I HincII HindIII HpaI KasI MmeI MslI
MspA1I NaeI NarI NgoMIV NspI PflMI PstI PvuII
SalI SfcI SfoI SgrAI SmlI SpeI SspI StuI
StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AleI AlfI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI BsaI BsaXI BseRI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I BtgI BtgZI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI EcoO109I EcoRV FalI FseI
FspI FspAI KpnI MfeI MluI MscI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SexAI SfiI SgfI SmaI SnaBI SphI SrfI
SwaI Tth111I XbaI XmaI ZraI