Restriction map for
pF1KB5649
(Linear) (Six-base) MAPSORT of: KIBB5649.seq Check: 3051 from: 1 to: 2217
KIBB5649.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 737 2217
Size: 737 1480
Acc65I G'GTAC_C
Cuts at: 0 463 2217
Size: 463 1754
AclI AA'CG_TT
Cuts at: 0 29 2217
Size: 29 2188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 864 1699 2217
Size: 71 793 835 518
Fragments arranged by size:
835 793 518 71
AflII C'TTAA_G
Cuts at: 0 985 2217
Size: 985 1232
AflIII A'CryG_T
Cuts at: 0 873 2217
Size: 873 1344
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2024 2056 2171 2203 2217
Size: 2024 32 115 32 14
Fragments arranged by size:
2024 115 32 32 14
AlwNI CAG_nnn'CTG
Cuts at: 0 2043 2217
Size: 2043 174
ApoI r'AATT_y
Cuts at: 0 1489 2217
Size: 1489 728
BamHI G'GATC_C
Cuts at: 0 77 158 1988 2217
Size: 77 81 1830 229
Fragments arranged by size:
1830 229 81 77
BanI G'GyrC_C
Cuts at: 0 366 463 775 990 2217
Size: 366 97 312 215 1227
Fragments arranged by size:
1227 366 312 215 97
BanII G_rGCy'C
Cuts at: 0 824 853 1482 2217
Size: 824 29 629 735
Fragments arranged by size:
824 735 629 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 870 2217
Size: 870 1347
BciVI GTATCCnnnnn_n'
Cuts at: 0 863 1524 2217
Size: 863 661 693
Fragments arranged by size:
863 693 661
BclI T'GATC_A
Cuts at: 0 135 1454 2217
Size: 135 1319 763
Fragments arranged by size:
1319 763 135
Bme1580I G_kGCm'C
Cuts at: 0 371 2217
Size: 371 1846
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 1106 2217
Size: 366 740 1111
Fragments arranged by size:
1111 740 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 2217
Size: 707 1510
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 2217
Size: 121 2096
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1506 1536 2171 2201 2217
Size: 1506 30 635 30 16
Fragments arranged by size:
1506 635 30 30 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1331 1550 2217
Size: 258 1073 219 667
Fragments arranged by size:
1073 667 258 219
BsiHKAI G_wGCw'C
Cuts at: 0 573 604 2217
Size: 573 31 1613
Fragments arranged by size:
1613 573 31
BsmI GAATG_Cn'
Cuts at: 0 1783 2217
Size: 1783 434
Bsp1286I G_dGCh'C
Cuts at: 0 371 573 604 824 853 1482 2217
Size: 371 202 31 220 29 629 735
Fragments arranged by size:
735 629 371 220 202 31 29
BsrDI GCAATG_nn'
Cuts at: 0 377 2217
Size: 377 1840
BsrGI T'GTAC_A
Cuts at: 0 422 2217
Size: 422 1795
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1318 2217
Size: 1318 899
BstYI r'GATC_y
Cuts at: 0 77 158 1497 1988 2217
Size: 77 81 1339 491 229
Fragments arranged by size:
1339 491 229 81 77
Bsu36I CC'TnA_GG
Cuts at: 0 579 2217
Size: 579 1638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 718 2158 2217
Size: 718 1440 59
Fragments arranged by size:
1440 718 59
BtsI GCAGTG_nn'
Cuts at: 0 295 1567 1654 1965 2217
Size: 295 1272 87 311 252
Fragments arranged by size:
1272 311 295 252 87
DraI TTT'AAA
Cuts at: 0 1785 2217
Size: 1785 432
EaeI y'GGCC_r
Cuts at: 0 399 2217
Size: 399 1818
EarI CTCTTCn'nnn_
Cuts at: 0 1667 2128 2217
Size: 1667 461 89
Fragments arranged by size:
1667 461 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 707 864 1699 2217
Size: 71 636 157 835 518
Fragments arranged by size:
835 636 518 157 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 578 2217
Size: 578 1639
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2134 2217
Size: 2134 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 120 152 322 354 1888 1920 2217
Size: 120 32 170 32 1534 32 297
Fragments arranged by size:
1534 297 170 120 32 32 32
HincII GTy'rAC
Cuts at: 0 528 1812 2217
Size: 528 1284 405
Fragments arranged by size:
1284 528 405
HindIII A'AGCT_T
Cuts at: 0 982 2217
Size: 982 1235
KpnI G_GTAC'C
Cuts at: 0 467 2217
Size: 467 1750
MfeI C'AATT_G
Cuts at: 0 1460 2217
Size: 1460 757
MscI TGG'CCA
Cuts at: 0 401 2217
Size: 401 1816
MslI CAynn'nnrTG
Cuts at: 0 481 2217
Size: 481 1736
MspA1I CmG'CkG
Cuts at: 0 49 2217
Size: 49 2168
NspI r_CATG'y
Cuts at: 0 877 2217
Size: 877 1340
PciI A'CATG_T
Cuts at: 0 873 2217
Size: 873 1344
PflMI CCAn_nnn'nTGG
Cuts at: 0 1511 2217
Size: 1511 706
PfoI T'CCnGG_A
Cuts at: 0 1892 2217
Size: 1892 325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2171 2203 2217
Size: 2171 32 14
Fragments arranged by size:
2171 32 14
PsiI TTA'TAA
Cuts at: 0 1067 2217
Size: 1067 1150
PstI C_TGCA'G
Cuts at: 0 224 1960 2217
Size: 224 1736 257
Fragments arranged by size:
1736 257 224
PvuII CAG'CTG
Cuts at: 0 49 2217
Size: 49 2168
ScaI AGT'ACT
Cuts at: 0 108 500 1578 2217
Size: 108 392 1078 639
Fragments arranged by size:
1078 639 392 108
SfcI C'TryA_G
Cuts at: 0 220 538 1148 1943 1956 2127 2217
Size: 220 318 610 795 13 171 90
Fragments arranged by size:
795 610 318 220 171 90 13
SmlI C'TyrA_G
Cuts at: 0 985 2217
Size: 985 1232
SpeI A'CTAG_T
Cuts at: 0 1306 2217
Size: 1306 911
SspI AAT'ATT
Cuts at: 0 2095 2217
Size: 2095 122
StuI AGG'CCT
Cuts at: 0 1589 2217
Size: 1589 628
StyI C'CwwG_G
Cuts at: 0 1446 1928 2217
Size: 1446 482 289
Fragments arranged by size:
1446 482 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 51 545 1125 2217
Size: 51 494 580 1092
Fragments arranged by size:
1092 580 494 51
TatI w'GTAC_w
Cuts at: 0 106 422 498 1576 2217
Size: 106 316 76 1078 641
Fragments arranged by size:
1078 641 316 106 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 828 860 961 993 2217
Size: 828 32 101 32 1224
Fragments arranged by size:
1224 828 101 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 588 2117 2217
Size: 588 1529 100
Fragments arranged by size:
1529 588 100
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApoI BamHI BanI BanII BbsI BciVI BclI Bme1580I
BmrI BpmI BsaI BsaXI BsgI BsiHKAI BsmI Bsp1286I
BsrDI BsrGI BstAPI BstYI Bsu36I BtgZI BtsI DraI
EaeI EarI Eco57MI EcoNI FalI Hin4I HincII HindIII
KpnI MfeI MscI MslI MspA1I NspI PciI PflMI
PfoI PpiI PsiI PstI PvuII ScaI SfcI SmlI
SpeI SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI