Restriction map for
pF1KB5655
(Linear) (Six-base) MAPSORT of: KIBB5655.seq Check: 7034 from: 1 to: 3684
KIBB5655.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 279 3684
Size: 279 3405
AccI GT'mk_AC
Cuts at: 0 1639 3684
Size: 1639 2045
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 242 409 1886 2860 3684
Size: 110 132 167 1477 974 824
Fragments arranged by size:
1477 974 824 167 132 110
AflII C'TTAA_G
Cuts at: 0 1819 3040 3684
Size: 1819 1221 644
Fragments arranged by size:
1819 1221 644
AflIII A'CryG_T
Cuts at: 0 1417 1634 1781 1842 3684
Size: 1417 217 147 61 1842
Fragments arranged by size:
1842 1417 217 147 61
AgeI A'CCGG_T
Cuts at: 0 721 3684
Size: 721 2963
AhdI GACnn_n'nnGTC
Cuts at: 0 2595 3684
Size: 2595 1089
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 3684
Size: 1051 32 2601
Fragments arranged by size:
2601 1051 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1603 3684
Size: 1603 2081
ApaLI G'TGCA_C
Cuts at: 0 266 3684
Size: 266 3418
ApoI r'AATT_y
Cuts at: 0 418 600 1108 1187 1810 1836 2064 2568
Size: 418 182 508 79 623 26 228 504
Cuts at: 2568 2697 2893 3406 3413 3684
Size: 129 196 513 7 271
Fragments arranged by size:
623 513 508 504 418 271 228 196
182 129 79 26 7
AseI AT'TA_AT
Cuts at: 0 1148 3684
Size: 1148 2536
AvaI C'yCGr_G
Cuts at: 0 173 3684
Size: 173 3511
BamHI G'GATC_C
Cuts at: 0 727 3684
Size: 727 2957
BanII G_rGCy'C
Cuts at: 0 534 852 3684
Size: 534 318 2832
Fragments arranged by size:
2832 534 318
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 223 230 1784 3684
Size: 115 108 7 1554 1900
Fragments arranged by size:
1900 1554 115 108 7
BbvCI CC'TCA_GC
Cuts at: 0 275 550 3684
Size: 275 275 3134
Fragments arranged by size:
3134 275 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1327 1361 3684
Size: 1327 34 2323
Fragments arranged by size:
2323 1327 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 814 3684
Size: 814 2870
BclI T'GATC_A
Cuts at: 0 975 3684
Size: 975 2709
BglII A'GATC_T
Cuts at: 0 742 3684
Size: 742 2942
BlpI GC'TnA_GC
Cuts at: 0 920 3684
Size: 920 2764
Bme1580I G_kGCm'C
Cuts at: 0 270 3163 3684
Size: 270 2893 521
Fragments arranged by size:
2893 521 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 3572 3684
Size: 3572 112
BmtI G_CTAG'C
Cuts at: 0 365 3684
Size: 365 3319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1990 3684
Size: 1990 1694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 401 506 533 1580 3245 3684
Size: 188 213 105 27 1047 1665 439
Fragments arranged by size:
1665 1047 439 213 188 105 27
Bpu10I CC'TnA_GC
Cuts at: 0 275 550 3684
Size: 275 275 3134
Fragments arranged by size:
3134 275 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1473 1978 2909 3684
Size: 1473 505 931 775
Fragments arranged by size:
1473 931 775 505
BsaAI yAC'GTr
Cuts at: 0 3164 3323 3684
Size: 3164 159 361
Fragments arranged by size:
3164 361 159
BsaWI w'CCGG_w
Cuts at: 0 721 3684
Size: 721 2963
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 234 264 798 828 1051 1081 1741 1771
Size: 234 30 534 30 223 30 660 30
Cuts at: 1771 3684
Size: 1913
Fragments arranged by size:
1913 660 534 234 223 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 926 1059 3133 3684
Size: 926 133 2074 551
Fragments arranged by size:
2074 926 551 133
BseYI C'CCAG_C
Cuts at: 0 1316 3684
Size: 1316 2368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3210 3684
Size: 3210 474
BsiEI CG_ry'CG
Cuts at: 0 2965 3684
Size: 2965 719
BsiHKAI G_wGCw'C
Cuts at: 0 270 1473 3468 3684
Size: 270 1203 1995 216
Fragments arranged by size:
1995 1203 270 216
BsmI GAATG_Cn'
Cuts at: 0 72 1496 1596 2829 3684
Size: 72 1424 100 1233 855
Fragments arranged by size:
1424 1233 855 100 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1740 3684
Size: 1740 1944
Bsp1286I G_dGCh'C
Cuts at: 0 270 534 852 1473 3163 3468 3684
Size: 270 264 318 621 1690 305 216
Fragments arranged by size:
1690 621 318 305 270 264 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 279 2410 3684
Size: 279 2131 1274
Fragments arranged by size:
2131 1274 279
BsrDI GCAATG_nn'
Cuts at: 0 1001 3016 3684
Size: 1001 2015 668
Fragments arranged by size:
2015 1001 668
BsrFI r'CCGG_y
Cuts at: 0 721 3684
Size: 721 2963
BsrGI T'GTAC_A
Cuts at: 0 639 3684
Size: 639 3045
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 3310 3684
Size: 162 3148 374
Fragments arranged by size:
3148 374 162
BstBI TT'CG_AA
Cuts at: 0 605 3684
Size: 605 3079
BstEII G'GTnAC_C
Cuts at: 0 946 3684
Size: 946 2738
BstYI r'GATC_y
Cuts at: 0 727 742 1940 2279 3277 3684
Size: 727 15 1198 339 998 407
Fragments arranged by size:
1198 998 727 407 339 15
Bsu36I CC'TnA_GG
Cuts at: 0 89 3116 3684
Size: 89 3027 568
Fragments arranged by size:
3027 568 89
BtgI C'CryG_G
Cuts at: 0 535 3296 3684
Size: 535 2761 388
Fragments arranged by size:
2761 535 388
BtsI GCAGTG_nn'
Cuts at: 0 244 2655 3684
Size: 244 2411 1029
Fragments arranged by size:
2411 1029 244
ClaI AT'CG_AT
Cuts at: 0 2961 3684
Size: 2961 723
DraI TTT'AAA
Cuts at: 0 1185 1809 3684
Size: 1185 624 1875
Fragments arranged by size:
1875 1185 624
DraIII CAC_nnn'GTG
Cuts at: 0 1389 3684
Size: 1389 2295
DrdI GACnn_nn'nnGTC
Cuts at: 0 679 3684
Size: 679 3005
EarI CTCTTCn'nnn_
Cuts at: 0 1013 1124 1231 1826 2684 3684
Size: 1013 111 107 595 858 1000
Fragments arranged by size:
1013 1000 858 595 111 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 3684
Size: 202 3482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 188 242 401 409 506 533 1580
Size: 110 78 54 159 8 97 27 1047
Cuts at: 1580 1886 2860 3245 3684
Size: 306 974 385 439
Fragments arranged by size:
1047 974 439 385 306 159 110 97
78 54 27 8
EcoO109I rG'GnC_Cy
Cuts at: 0 706 1254 3684
Size: 706 548 2430
Fragments arranged by size:
2430 706 548
EcoRI G'AATT_C
Cuts at: 0 3413 3684
Size: 3413 271
EcoRV GAT'ATC
Cuts at: 0 755 3684
Size: 755 2929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 247 1079 3684
Size: 247 832 2605
Fragments arranged by size:
2605 832 247
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 85 117 744 776 798 830
Size: 5 32 48 32 627 32 22 32
Cuts at: 830 1621 1653 1710 1741 1742 1771 1773 1803
Size: 791 32 57 31 1 29 2 30
Cuts at: 1803 2022 2054 2581 2613 3684
Size: 219 32 527 32 1071
Fragments arranged by size:
1071 791 627 527 219 57 48 32
32 32 32 32 32 32 31 30
29 22 5 2 1
HincII GTy'rAC
Cuts at: 0 841 3684
Size: 841 2843
HindIII A'AGCT_T
Cuts at: 0 3235 3505 3684
Size: 3235 270 179
Fragments arranged by size:
3235 270 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 483 2477 2696 3684
Size: 456 27 1994 219 988
Fragments arranged by size:
1994 988 456 219 27
MslI CAynn'nnrTG
Cuts at: 0 3323 3684
Size: 3323 361
MspA1I CmG'CkG
Cuts at: 0 901 1600 3684
Size: 901 699 2084
Fragments arranged by size:
2084 901 699
NcoI C'CATG_G
Cuts at: 0 3296 3684
Size: 3296 388
NdeI CA'TA_TG
Cuts at: 0 1992 3684
Size: 1992 1692
NheI G'CTAG_C
Cuts at: 0 361 3684
Size: 361 3323
NspI r_CATG'y
Cuts at: 0 1206 1421 1638 1785 1846 3684
Size: 1206 215 217 147 61 1838
Fragments arranged by size:
1838 1206 217 215 147 61
PciI A'CATG_T
Cuts at: 0 1417 1634 1781 1842 3684
Size: 1417 217 147 61 1842
Fragments arranged by size:
1842 1417 217 147 61
PflMI CCAn_nnn'nTGG
Cuts at: 0 3400 3684
Size: 3400 284
PfoI T'CCnGG_A
Cuts at: 0 378 816 3684
Size: 378 438 2868
Fragments arranged by size:
2868 438 378
PmlI CAC'GTG
Cuts at: 0 3164 3684
Size: 3164 520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 3684
Size: 1051 32 2601
Fragments arranged by size:
2601 1051 32
PpuMI rG'GwC_Cy
Cuts at: 0 706 1254 3684
Size: 706 548 2430
Fragments arranged by size:
2430 706 548
PshAI GACnn'nnGTC
Cuts at: 0 2365 3684
Size: 2365 1319
PsiI TTA'TAA
Cuts at: 0 2203 3684
Size: 2203 1481
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2461 2493 3171 3203 3684
Size: 2461 32 678 32 481
Fragments arranged by size:
2461 678 481 32 32
PstI C_TGCA'G
Cuts at: 0 1016 3202 3684
Size: 1016 2186 482
Fragments arranged by size:
2186 1016 482
PvuI CG_AT'CG
Cuts at: 0 2965 3684
Size: 2965 719
PvuII CAG'CTG
Cuts at: 0 901 1600 3684
Size: 901 699 2084
Fragments arranged by size:
2084 901 699
SapI GCTCTTCn'nnn_
Cuts at: 0 1231 2684 3684
Size: 1231 1453 1000
Fragments arranged by size:
1453 1231 1000
ScaI AGT'ACT
Cuts at: 0 2259 3684
Size: 2259 1425
SexAI A'CCwGG_T
Cuts at: 0 646 677 3339 3684
Size: 646 31 2662 345
Fragments arranged by size:
2662 646 345 31
SfcI C'TryA_G
Cuts at: 0 768 1012 1214 3132 3198 3684
Size: 768 244 202 1918 66 486
Fragments arranged by size:
1918 768 486 244 202 66
SmlI C'TyrA_G
Cuts at: 0 1488 1819 1957 2924 3040 3684
Size: 1488 331 138 967 116 644
Fragments arranged by size:
1488 967 644 331 138 116
SnaBI TAC'GTA
Cuts at: 0 3323 3684
Size: 3323 361
SpeI A'CTAG_T
Cuts at: 0 2044 3684
Size: 2044 1640
SspI AAT'ATT
Cuts at: 0 1032 3528 3684
Size: 1032 2496 156
Fragments arranged by size:
2496 1032 156
StyI C'CwwG_G
Cuts at: 0 3296 3684
Size: 3296 388
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3309 3684
Size: 3309 375
TatI w'GTAC_w
Cuts at: 0 639 1152 1545 1631 2115 2257 3443 3684
Size: 639 513 393 86 484 142 1186 241
Fragments arranged by size:
1186 639 513 484 393 241 142 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 793 825 3470 3502 3684
Size: 793 32 2645 32 182
Fragments arranged by size:
2645 793 182 32 32
XbaI T'CTAG_A
Cuts at: 0 1473 3684
Size: 1473 2211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 3684
Size: 666 3018
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AvaI BamHI BanII BbsI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII MmeI MslI MspA1I NcoI NdeI NheI NspI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SapI ScaI SexAI SfcI
SmlI SnaBI SpeI SspI StyI TaqII TatI TstI
XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AleI AlfI ApaI AscI
AsiSI AvrII BaeI BanI BbeI BfrBI BglI BmgBI
BsaI BsaBI BsaHI BsiWI BspEI BspHI BsrBI BssHII
BssSI BstXI BstZ17I BtgZI CspCI EaeI EagI EcoICRI
EcoNI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NotI NruI
NsiI PacI PasI PmeI PspOMI PspXI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SmaI SphI SrfI StuI SwaI Tth111I XhoI XmaI
XmnI ZraI