Restriction map for
pF1KB5659
(Linear) (Six-base) MAPSORT of: KIBB5659.seq Check: 8188 from: 1 to: 924
KIBB5659.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 0 369 924
Size: 369 555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 924
Size: 526 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 260 924
Size: 260 664
AlwNI CAG_nnn'CTG
Cuts at: 0 384 924
Size: 384 540
ApaI G_GGCC'C
Cuts at: 0 825 924
Size: 825 99
ApoI r'AATT_y
Cuts at: 0 499 924
Size: 499 425
BamHI G'GATC_C
Cuts at: 0 259 924
Size: 259 665
BanII G_rGCy'C
Cuts at: 0 362 825 869 924
Size: 362 463 44 55
Fragments arranged by size:
463 362 55 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 230 264 924
Size: 230 34 660
Fragments arranged by size:
660 230 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 560 924
Size: 560 364
BglII A'GATC_T
Cuts at: 0 86 924
Size: 86 838
Bme1580I G_kGCm'C
Cuts at: 0 702 825 924
Size: 702 123 99
Fragments arranged by size:
702 123 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 322 924
Size: 322 602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 724 924
Size: 477 247 200
Fragments arranged by size:
477 247 200
Bpu10I CC'TnA_GC
Cuts at: 0 70 577 924
Size: 70 507 347
Fragments arranged by size:
507 347 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 924
Size: 640 284
BsaI GGTCTCn'nnnn_
Cuts at: 0 727 924
Size: 727 197
BsaHI Gr'CG_yC
Cuts at: 0 366 924
Size: 366 558
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 853 856 924
Size: 853 3 68
Fragments arranged by size:
853 68 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 924
Size: 146 778
BsiEI CG_ry'CG
Cuts at: 0 44 117 924
Size: 44 73 807
Fragments arranged by size:
807 73 44
BsiHKAI G_wGCw'C
Cuts at: 0 362 869 913 924
Size: 362 507 44 11
Fragments arranged by size:
507 362 44 11
Bsp1286I G_dGCh'C
Cuts at: 0 362 702 825 869 913 924
Size: 362 340 123 44 44 11
Fragments arranged by size:
362 340 123 44 44 11
BsrDI GCAATG_nn'
Cuts at: 0 175 924
Size: 175 749
BsrFI r'CCGG_y
Cuts at: 0 112 924
Size: 112 812
BsrGI T'GTAC_A
Cuts at: 0 784 924
Size: 784 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 410 924
Size: 410 514
BstYI r'GATC_y
Cuts at: 0 86 259 924
Size: 86 173 665
Fragments arranged by size:
665 173 86
BtsI GCAGTG_nn'
Cuts at: 0 288 924
Size: 288 636
EaeI y'GGCC_r
Cuts at: 0 114 924
Size: 114 810
EagI C'GGCC_G
Cuts at: 0 114 924
Size: 114 810
EarI CTCTTCn'nnn_
Cuts at: 0 59 924
Size: 59 865
EcoICRI GAG'CTC
Cuts at: 0 360 867 924
Size: 360 507 57
Fragments arranged by size:
507 360 57
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 526 724 924
Size: 477 49 198 200
Fragments arranged by size:
477 200 198 49
EcoO109I rG'GnC_Cy
Cuts at: 0 821 924
Size: 821 103
EcoRI G'AATT_C
Cuts at: 0 499 924
Size: 499 425
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 251 283 289 321 356 388 924
Size: 251 32 6 32 35 32 536
Fragments arranged by size:
536 251 35 32 32 32 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 387 924
Size: 371 16 537
Fragments arranged by size:
537 371 16
MslI CAynn'nnrTG
Cuts at: 0 242 924
Size: 242 682
MspA1I CmG'CkG
Cuts at: 0 755 889 924
Size: 755 134 35
Fragments arranged by size:
755 134 35
NaeI GCC'GGC
Cuts at: 0 114 924
Size: 114 810
NgoMIV G'CCGG_C
Cuts at: 0 112 924
Size: 112 812
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 924
Size: 384 540
PfoI T'CCnGG_A
Cuts at: 0 860 924
Size: 860 64
PspOMI G'GGCC_C
Cuts at: 0 821 924
Size: 821 103
PvuII CAG'CTG
Cuts at: 0 889 924
Size: 889 35
SacI G_AGCT'C
Cuts at: 0 362 869 924
Size: 362 507 55
Fragments arranged by size:
507 362 55
SapI GCTCTTCn'nnn_
Cuts at: 0 59 924
Size: 59 865
SfcI C'TryA_G
Cuts at: 0 432 924
Size: 432 492
SmlI C'TyrA_G
Cuts at: 0 655 924
Size: 655 269
SspI AAT'ATT
Cuts at: 0 540 924
Size: 540 384
StuI AGG'CCT
Cuts at: 0 189 924
Size: 189 735
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 751 924
Size: 751 173
TatI w'GTAC_w
Cuts at: 0 784 924
Size: 784 140
ZraI GAC'GTC
Cuts at: 0 367 924
Size: 367 557
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApaI ApoI BamHI BanII
BcgI BciVI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrDI
BsrFI BsrGI BstXI BstYI BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I MmeI MslI MspA1I
NaeI NgoMIV PflMI PfoI PspOMI PvuII SacI SapI
SfcI SmlI SspI StuI TaqII TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI
BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI