Restriction map for
pF1KB5666
(Linear) (Six-base) MAPSORT of: KIBB5666.seq Check: 4143 from: 1 to: 2901
KIBB5666.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 343 1568 2901
Size: 343 1225 1333
Fragments arranged by size:
1333 1225 343
AatII G_ACGT'C
Cuts at: 0 356 2901
Size: 356 2545
AccI GT'mk_AC
Cuts at: 0 2387 2901
Size: 2387 514
Acc65I G'GTAC_C
Cuts at: 0 2528 2901
Size: 2528 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1818 2181 2185 2901
Size: 1818 363 4 716
Fragments arranged by size:
1818 716 363 4
AflIII A'CryG_T
Cuts at: 0 1745 2901
Size: 1745 1156
AgeI A'CCGG_T
Cuts at: 0 1580 2901
Size: 1580 1321
AhdI GACnn_n'nnGTC
Cuts at: 0 1113 2901
Size: 1113 1788
AleI CACnn'nnGTG
Cuts at: 0 1603 2901
Size: 1603 1298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 2901
Size: 84 2817
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 526 558 1567 1599 2901
Size: 526 32 1009 32 1302
Fragments arranged by size:
1302 1009 526 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1497 2751 2901
Size: 1497 1254 150
Fragments arranged by size:
1497 1254 150
ApaI G_GGCC'C
Cuts at: 0 912 967 2808 2901
Size: 912 55 1841 93
Fragments arranged by size:
1841 912 93 55
ApaLI G'TGCA_C
Cuts at: 0 69 490 2901
Size: 69 421 2411
Fragments arranged by size:
2411 421 69
ApoI r'AATT_y
Cuts at: 0 1735 2901
Size: 1735 1166
AseI AT'TA_AT
Cuts at: 0 1985 2901
Size: 1985 916
AvaI C'yCGr_G
Cuts at: 0 2801 2901
Size: 2801 100
BamHI G'GATC_C
Cuts at: 0 44 2516 2901
Size: 44 2472 385
Fragments arranged by size:
2472 385 44
BanI G'GyrC_C
Cuts at: 0 1054 1438 2528 2797 2901
Size: 1054 384 1090 269 104
Fragments arranged by size:
1090 1054 384 269 104
BanII G_rGCy'C
Cuts at: 0 316 473 505 912 967 1280 1821 2808
Size: 316 157 32 407 55 313 541 987
Cuts at: 2808 2901
Size: 93
Fragments arranged by size:
987 541 407 316 313 157 93 55
32
BbeI G_GCGC'C
Cuts at: 0 1058 1442 2901
Size: 1058 384 1459
Fragments arranged by size:
1459 1058 384
BbsI GAAGACnn'nnnn_
Cuts at: 0 785 2833 2901
Size: 785 2048 68
Fragments arranged by size:
2048 785 68
BbvCI CC'TCA_GC
Cuts at: 0 159 391 2901
Size: 159 232 2510
Fragments arranged by size:
2510 232 159
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 60 94 2245 2279 2382 2416 2901
Size: 60 34 2151 34 103 34 485
Fragments arranged by size:
2151 485 103 60 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1656 2901
Size: 1656 1245
BglI GCCn_nnn'nGGC
Cuts at: 0 688 928 2901
Size: 688 240 1973
Fragments arranged by size:
1973 688 240
Bme1580I G_kGCm'C
Cuts at: 0 73 404 494 912 967 1760 1977 2013
Size: 73 331 90 418 55 793 217 36
Cuts at: 2013 2808 2901
Size: 795 93
Fragments arranged by size:
795 793 418 331 217 93 90 73
55 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 1077 2901
Size: 911 166 1824
Fragments arranged by size:
1824 911 166
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2331 2901
Size: 2331 570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1107 1446 1935 2238 2773 2830 2842
Size: 300 807 339 489 303 535 57 12
Cuts at: 2842 2860 2901
Size: 18 41
Fragments arranged by size:
807 535 489 339 303 300 57 41
18 12
Bpu10I CC'TnA_GC
Cuts at: 0 159 391 2095 2104 2533 2793 2901
Size: 159 232 1704 9 429 260 108
Fragments arranged by size:
1704 429 260 232 159 108 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 2901
Size: 481 2420
BsaI GGTCTCn'nnnn_
Cuts at: 0 261 1937 2319 2901
Size: 261 1676 382 582
Fragments arranged by size:
1676 582 382 261
BsaAI yAC'GTr
Cuts at: 0 830 867 1248 1746 2901
Size: 830 37 381 498 1155
Fragments arranged by size:
1155 830 498 381 37
BsaBI GATnn'nnATC
Cuts at: 0 901 2901
Size: 901 2000
BsaHI Gr'CG_yC
Cuts at: 0 353 1055 1418 1439 1501 1991 2573 2901
Size: 353 702 363 21 62 490 582 328
Fragments arranged by size:
702 582 490 363 353 328 62 21
BsaWI w'CCGG_w
Cuts at: 0 899 1580 2561 2901
Size: 899 681 981 340
Fragments arranged by size:
981 899 681 340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1408 1438 2407 2437 2901
Size: 1408 30 969 30 464
Fragments arranged by size:
1408 969 464 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1110 1341 2320 2431 2434 2901
Size: 1110 231 979 111 3 467
Fragments arranged by size:
1110 979 467 231 111 3
BseYI C'CCAG_C
Cuts at: 0 434 760 2901
Size: 434 326 2141
Fragments arranged by size:
2141 434 326
BsiHKAI G_wGCw'C
Cuts at: 0 73 494 1694 2876 2901
Size: 73 421 1200 1182 25
Fragments arranged by size:
1200 1182 421 73 25
Bsp1286I G_dGCh'C
Cuts at: 0 73 316 404 473 494 505 912 967
Size: 73 243 88 69 21 11 407 55
Cuts at: 967 1280 1694 1760 1821 1977 2013 2808 2876
Size: 313 414 66 61 156 36 795 68
Cuts at: 2876 2901
Size: 25
Fragments arranged by size:
795 414 407 313 243 156 88 73
69 68 66 61 55 36 25 21
11
BspEI T'CCGG_A
Cuts at: 0 899 2561 2901
Size: 899 1662 340
Fragments arranged by size:
1662 899 340
BspHI T'CATG_A
Cuts at: 0 1161 1616 2901
Size: 1161 455 1285
Fragments arranged by size:
1285 1161 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 343 1568 2163 2381 2901
Size: 343 1225 595 218 520
Fragments arranged by size:
1225 595 520 343 218
BsrBI CCG'CTC
Cuts at: 0 1140 2132 2901
Size: 1140 992 769
Fragments arranged by size:
1140 992 769
BsrDI GCAATG_nn'
Cuts at: 0 609 923 1854 2901
Size: 609 314 931 1047
Fragments arranged by size:
1047 931 609 314
BsrFI r'CCGG_y
Cuts at: 0 678 1051 1392 1580 2901
Size: 678 373 341 188 1321
Fragments arranged by size:
1321 678 373 341 188
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1339 2279 2901
Size: 1339 940 622
Fragments arranged by size:
1339 940 622
BstEII G'GTnAC_C
Cuts at: 0 1077 1194 2901
Size: 1077 117 1707
Fragments arranged by size:
1707 1077 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 192 2901
Size: 30 162 2709
Fragments arranged by size:
2709 162 30
BstYI r'GATC_y
Cuts at: 0 44 902 2225 2516 2901
Size: 44 858 1323 291 385
Fragments arranged by size:
1323 858 385 291 44
Bsu36I CC'TnA_GG
Cuts at: 0 1450 2901
Size: 1450 1451
BtgI C'CryG_G
Cuts at: 0 134 936 1058 2901
Size: 134 802 122 1843
Fragments arranged by size:
1843 802 134 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 63 2901
Size: 63 2838
BtsI GCAGTG_nn'
Cuts at: 0 1340 2338 2717 2901
Size: 1340 998 379 184
Fragments arranged by size:
1340 998 379 184
DraIII CAC_nnn'GTG
Cuts at: 0 408 2901
Size: 408 2493
DrdI GACnn_nn'nnGTC
Cuts at: 0 735 2901
Size: 735 2166
EaeI y'GGCC_r
Cuts at: 0 2 182 620 680 1952 2003 2901
Size: 2 180 438 60 1272 51 898
Fragments arranged by size:
1272 898 438 180 60 51 2
EarI CTCTTCn'nnn_
Cuts at: 0 1837 1901 2056 2057 2191 2901
Size: 1837 64 155 1 134 710
Fragments arranged by size:
1837 710 155 134 64 1
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 144 2901
Size: 144 2757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1107 1446 1818 1935 2181 2185 2238
Size: 300 807 339 372 117 246 4 53
Cuts at: 2238 2773 2830 2842 2860 2901
Size: 535 57 12 18 41
Fragments arranged by size:
807 535 372 339 300 246 117 57
53 41 18 12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 394 511 1474 1564 1684 2704 2901
Size: 394 117 963 90 120 1020 197
Fragments arranged by size:
1020 963 394 197 120 117 90
EcoO109I rG'GnC_Cy
Cuts at: 0 294 723 757 964 1494 1676 1857 2019
Size: 294 429 34 207 530 182 181 162
Cuts at: 2019 2805 2901
Size: 786 96
Fragments arranged by size:
786 530 429 294 207 182 181 162
96 34
EcoRI G'AATT_C
Cuts at: 0 1735 2901
Size: 1735 1166
HaeII r_GCGC'y
Cuts at: 0 1058 1442 2038 2901
Size: 1058 384 596 863
Fragments arranged by size:
1058 863 596 384
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 1099 1131 1408 1440 1914 1946
Size: 260 32 807 32 277 32 474 32
Cuts at: 1946 2563 2595 2901
Size: 617 32 306
Fragments arranged by size:
807 617 474 306 277 260 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 2656 2901
Size: 2656 245
KasI G'GCGC_C
Cuts at: 0 1054 1438 2901
Size: 1054 384 1463
Fragments arranged by size:
1463 1054 384
KpnI G_GTAC'C
Cuts at: 0 2532 2901
Size: 2532 369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 764 1614 2309 2720 2901
Size: 764 850 695 411 181
Fragments arranged by size:
850 764 695 411 181
MscI TGG'CCA
Cuts at: 0 4 622 2005 2901
Size: 4 618 1383 896
Fragments arranged by size:
1383 896 618 4
MslI CAynn'nnrTG
Cuts at: 0 1603 1657 2901
Size: 1603 54 1244
Fragments arranged by size:
1603 1244 54
MspA1I CmG'CkG
Cuts at: 0 1362 1625 1935 2751 2901
Size: 1362 263 310 816 150
Fragments arranged by size:
1362 816 310 263 150
NaeI GCC'GGC
Cuts at: 0 680 1053 1394 2901
Size: 680 373 341 1507
Fragments arranged by size:
1507 680 373 341
NarI GG'CG_CC
Cuts at: 0 1055 1439 2901
Size: 1055 384 1462
Fragments arranged by size:
1462 1055 384
NcoI C'CATG_G
Cuts at: 0 1058 2901
Size: 1058 1843
NgoMIV G'CCGG_C
Cuts at: 0 678 1051 1392 2901
Size: 678 373 341 1509
Fragments arranged by size:
1509 678 373 341
NspI r_CATG'y
Cuts at: 0 747 2079 2901
Size: 747 1332 822
Fragments arranged by size:
1332 822 747
PasI CC'CwG_GG
Cuts at: 0 30 2081 2406 2901
Size: 30 2051 325 495
Fragments arranged by size:
2051 495 325 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 1205 2216 2901
Size: 1205 1011 685
Fragments arranged by size:
1205 1011 685
PpuMI rG'GwC_Cy
Cuts at: 0 723 757 1676 1857 2019 2901
Size: 723 34 919 181 162 882
Fragments arranged by size:
919 882 723 181 162 34
PspOMI G'GGCC_C
Cuts at: 0 908 963 2804 2901
Size: 908 55 1841 97
Fragments arranged by size:
1841 908 97 55
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 2901
Size: 1522 32 1347
Fragments arranged by size:
1522 1347 32
PstI C_TGCA'G
Cuts at: 0 645 1565 1637 1940 2503 2747 2901
Size: 645 920 72 303 563 244 154
Fragments arranged by size:
920 645 563 303 244 154 72
PvuII CAG'CTG
Cuts at: 0 1935 2751 2901
Size: 1935 816 150
Fragments arranged by size:
1935 816 150
SanDI GG'GwC_CC
Cuts at: 0 1676 2901
Size: 1676 1225
SbfI CC_TGCA'GG
Cuts at: 0 1565 2901
Size: 1565 1336
SexAI A'CCwGG_T
Cuts at: 0 482 1190 2901
Size: 482 708 1711
Fragments arranged by size:
1711 708 482
SfcI C'TryA_G
Cuts at: 0 641 1428 1561 1633 1936 2499 2743 2901
Size: 641 787 133 72 303 563 244 158
Fragments arranged by size:
787 641 563 303 244 158 133 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 688 2901
Size: 688 2213
SfoI GGC'GCC
Cuts at: 0 1056 1440 2901
Size: 1056 384 1461
Fragments arranged by size:
1461 1056 384
SgrAI Cr'CCGG_yG
Cuts at: 0 1051 2901
Size: 1051 1850
SmaI CCC'GGG
Cuts at: 0 2803 2901
Size: 2803 98
SmlI C'TyrA_G
Cuts at: 0 496 2901
Size: 496 2405
StyI C'CwwG_G
Cuts at: 0 5 191 719 1058 2901
Size: 5 186 528 339 1843
Fragments arranged by size:
1843 528 339 186 5
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1249 2838 2901
Size: 1249 1589 63
Fragments arranged by size:
1589 1249 63
TatI w'GTAC_w
Cuts at: 0 95 590 2072 2249 2901
Size: 95 495 1482 177 652
Fragments arranged by size:
1482 652 495 177 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 756 788 1348 1380 2407 2439 2901
Size: 756 32 560 32 1027 32 462
Fragments arranged by size:
1027 756 560 462 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2635 2901
Size: 2635 266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 834 2901
Size: 480 354 2067
Fragments arranged by size:
2067 480 354
XmaI C'CCGG_G
Cuts at: 0 2801 2901
Size: 2801 100
XmnI GAAnn'nnTTC
Cuts at: 0 658 2901
Size: 658 2243
ZraI GAC'GTC
Cuts at: 0 354 2901
Size: 354 2547
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HindIII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PpuMI PspOMI PsrI PstI PvuII SanDI SbfI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AscI AsiSI AvrII BaeI BclI
BfrBI BglII BlpI BmgBI BmtI BsgI BsiEI BsiWI
BsmI BsmBI BsrGI BssHII BssSI BstBI BstZ17I ClaI
CspCI DraI EagI EcoICRI EcoRV FalI FseI FspI
FspAI HincII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SacI SacII SalI
SapI ScaI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI