Restriction map for
pF1KB5669
(Linear) (Six-base) MAPSORT of: KIBB5669.seq Check: 2551 from: 1 to: 1047
KIBB5669.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 0 590 1047
Size: 590 457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 913 1047
Size: 165 748 134
Fragments arranged by size:
748 165 134
AflIII A'CryG_T
Cuts at: 0 503 1047
Size: 503 544
AlwNI CAG_nnn'CTG
Cuts at: 0 342 1047
Size: 342 705
ApoI r'AATT_y
Cuts at: 0 124 268 457 795 1047
Size: 124 144 189 338 252
Fragments arranged by size:
338 252 189 144 124
AseI AT'TA_AT
Cuts at: 0 227 1047
Size: 227 820
AvaI C'yCGr_G
Cuts at: 0 103 1047
Size: 103 944
BamHI G'GATC_C
Cuts at: 0 6 148 1047
Size: 6 142 899
Fragments arranged by size:
899 142 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 1047
Size: 506 541
BfrBI ATG'CAT
Cuts at: 0 966 1047
Size: 966 81
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1047
Size: 34 1013
BlpI GC'TnA_GC
Cuts at: 0 257 1047
Size: 257 790
Bme1580I G_kGCm'C
Cuts at: 0 1015 1047
Size: 1015 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 92 1047
Size: 92 955
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 150 1047
Size: 76 74 897
Fragments arranged by size:
897 76 74
BsaWI w'CCGG_w
Cuts at: 0 432 462 1047
Size: 432 30 585
Fragments arranged by size:
585 432 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 302 1047
Size: 97 205 745
Fragments arranged by size:
745 205 97
BsiEI CG_ry'CG
Cuts at: 0 70 1047
Size: 70 977
BsmI GAATG_Cn'
Cuts at: 0 929 1047
Size: 929 118
Bsp1286I G_dGCh'C
Cuts at: 0 1015 1047
Size: 1015 32
BsrFI r'CCGG_y
Cuts at: 0 28 1047
Size: 28 1019
BsrGI T'GTAC_A
Cuts at: 0 441 1047
Size: 441 606
BstBI TT'CG_AA
Cuts at: 0 921 1047
Size: 921 126
BstYI r'GATC_y
Cuts at: 0 6 148 1047
Size: 6 142 899
Fragments arranged by size:
899 142 6
Bsu36I CC'TnA_GG
Cuts at: 0 611 1047
Size: 611 436
BtgI C'CryG_G
Cuts at: 0 38 64 1047
Size: 38 26 983
Fragments arranged by size:
983 38 26
EaeI y'GGCC_r
Cuts at: 0 67 132 1047
Size: 67 65 915
Fragments arranged by size:
915 67 65
EagI C'GGCC_G
Cuts at: 0 67 1047
Size: 67 980
EarI CTCTTCn'nnn_
Cuts at: 0 304 999 1047
Size: 304 695 48
Fragments arranged by size:
695 304 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 150 165 913 1047
Size: 76 74 15 748 134
Fragments arranged by size:
748 134 76 74 15
EcoRI G'AATT_C
Cuts at: 0 268 1047
Size: 268 779
HincII GTy'rAC
Cuts at: 0 730 1047
Size: 730 317
HindIII A'AGCT_T
Cuts at: 0 259 274 1047
Size: 259 15 773
Fragments arranged by size:
773 259 15
MscI TGG'CCA
Cuts at: 0 134 1047
Size: 134 913
MspA1I CmG'CkG
Cuts at: 0 66 543 1047
Size: 66 477 504
Fragments arranged by size:
504 477 66
NaeI GCC'GGC
Cuts at: 0 30 1047
Size: 30 1017
NdeI CA'TA_TG
Cuts at: 0 789 1047
Size: 789 258
NgoMIV G'CCGG_C
Cuts at: 0 28 1047
Size: 28 1019
NsiI A_TGCA'T
Cuts at: 0 968 1047
Size: 968 79
NspI r_CATG'y
Cuts at: 0 970 1047
Size: 970 77
PsiI TTA'TAA
Cuts at: 0 329 1047
Size: 329 718
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 284 316 1047
Size: 284 32 731
Fragments arranged by size:
731 284 32
PvuII CAG'CTG
Cuts at: 0 543 1047
Size: 543 504
SacII CC_GC'GG
Cuts at: 0 67 1047
Size: 67 980
SphI G_CATG'C
Cuts at: 0 970 1047
Size: 970 77
SspI AAT'ATT
Cuts at: 0 777 1047
Size: 777 270
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 926 1047
Size: 926 121
TatI w'GTAC_w
Cuts at: 0 441 1047
Size: 441 606
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI AseI AvaI BamHI
BbsI BfrBI BglI BlpI Bme1580I BmrI BpmI BsaWI
BseRI BsiEI BsmI Bsp1286I BsrFI BsrGI BstBI BstYI
Bsu36I BtgI EaeI EagI EarI Eco57MI EcoRI HincII
HindIII MscI MspA1I NaeI NdeI NgoMIV NsiI NspI
PsiI PsrI PvuII SacII SphI SspI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI
MfeI MluI MmeI MslI NarI NcoI NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI StyI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI