Restriction map for
pF1KB5675
(Linear) (Six-base) MAPSORT of: KIBB5675.seq Check: 354 from: 1 to: 3033
KIBB5675.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 206 699 1218 2711 3033
Size: 206 493 519 1493 322
Fragments arranged by size:
1493 519 493 322 206
AclI AA'CG_TT
Cuts at: 0 1843 3033
Size: 1843 1190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1707 1968 2473 3033
Size: 754 953 261 505 560
Fragments arranged by size:
953 754 560 505 261
AflIII A'CryG_T
Cuts at: 0 977 3033
Size: 977 2056
AgeI A'CCGG_T
Cuts at: 0 1165 3033
Size: 1165 1868
AleI CACnn'nnGTG
Cuts at: 0 284 494 763 2866 3033
Size: 284 210 269 2103 167
Fragments arranged by size:
2103 284 269 210 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 413 1648 2701 2777 3033
Size: 413 1235 1053 76 256
Fragments arranged by size:
1235 1053 413 256 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1678 1710 3033
Size: 1678 32 1323
Fragments arranged by size:
1678 1323 32
AlwNI CAG_nnn'CTG
Cuts at: 0 696 831 1499 3033
Size: 696 135 668 1534
Fragments arranged by size:
1534 696 668 135
ApaI G_GGCC'C
Cuts at: 0 2728 3033
Size: 2728 305
ApaLI G'TGCA_C
Cuts at: 0 193 1298 2065 2437 2698 3033
Size: 193 1105 767 372 261 335
Fragments arranged by size:
1105 767 372 335 261 193
AvaI C'yCGr_G
Cuts at: 0 140 602 853 2616 2641 3033
Size: 140 462 251 1763 25 392
Fragments arranged by size:
1763 462 392 251 140 25
BanI G'GyrC_C
Cuts at: 0 4 383 437 496 656 1351 1746 1972
Size: 4 379 54 59 160 695 395 226
Cuts at: 1972 2123 2519 2626 3033
Size: 151 396 107 407
Fragments arranged by size:
695 407 396 395 379 226 160 151
107 59 54 4
BanII G_rGCy'C
Cuts at: 0 952 2028 2309 2432 2649 2728 3033
Size: 952 1076 281 123 217 79 305
Fragments arranged by size:
1076 952 305 281 217 123 79
BbeI G_GCGC'C
Cuts at: 0 8 441 660 2523 2630 3033
Size: 8 433 219 1863 107 403
Fragments arranged by size:
1863 433 403 219 107 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 2050 3033
Size: 2050 983
BbvCI CC'TCA_GC
Cuts at: 0 1210 3033
Size: 1210 1823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1305 1339 3033
Size: 1305 34 1694
Fragments arranged by size:
1694 1305 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 145 3033
Size: 145 2888
BglI GCCn_nnn'nGGC
Cuts at: 0 325 3033
Size: 325 2708
BlpI GC'TnA_GC
Cuts at: 0 1507 3033
Size: 1507 1526
Bme1580I G_kGCm'C
Cuts at: 0 197 1302 1637 2069 2441 2702 2728 3033
Size: 197 1105 335 432 372 261 26 305
Fragments arranged by size:
1105 432 372 335 305 261 197 26
BmgBI CAC'GTC
Cuts at: 0 222 3033
Size: 222 2811
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 2910 3033
Size: 630 2280 123
Fragments arranged by size:
2280 630 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 556 718 1264 1312 2149 2230 2278
Size: 463 93 162 546 48 837 81 48
Cuts at: 2278 2446 2880 3033
Size: 168 434 153
Fragments arranged by size:
837 546 463 434 168 162 153 93
81 48 48
Bpu10I CC'TnA_GC
Cuts at: 0 1210 2791 3033
Size: 1210 1581 242
Fragments arranged by size:
1581 1210 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2835 2887 3033
Size: 2835 52 146
Fragments arranged by size:
2835 146 52
BsaI GGTCTCn'nnnn_
Cuts at: 0 413 2851 3033
Size: 413 2438 182
Fragments arranged by size:
2438 413 182
BsaAI yAC'GTr
Cuts at: 0 354 1368 3033
Size: 354 1014 1665
Fragments arranged by size:
1665 1014 354
BsaBI GATnn'nnATC
Cuts at: 0 2347 3033
Size: 2347 686
BsaHI Gr'CG_yC
Cuts at: 0 5 438 657 1340 2520 2627 3033
Size: 5 433 219 683 1180 107 406
Fragments arranged by size:
1180 683 433 406 219 107 5
BsaWI w'CCGG_w
Cuts at: 0 1165 1288 1683 2779 3033
Size: 1165 123 395 1096 254
Fragments arranged by size:
1165 1096 395 254 123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 697 727 1678 1708 2122 2152 2482 2512
Size: 697 30 951 30 414 30 330 30
Cuts at: 2512 2555 2585 2743 2773 2812 2842 3033
Size: 43 30 158 30 39 30 191
Fragments arranged by size:
951 697 414 330 191 158 43 39
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2277 2639 3033
Size: 2277 362 394
Fragments arranged by size:
2277 394 362
BseYI C'CCAG_C
Cuts at: 0 461 803 1403 1636 1657 2021 2720 3033
Size: 461 342 600 233 21 364 699 313
Fragments arranged by size:
699 600 461 364 342 313 233 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 310 2077 3033
Size: 193 117 1767 956
Fragments arranged by size:
1767 956 193 117
BsiEI CG_ry'CG
Cuts at: 0 746 1186 3033
Size: 746 440 1847
Fragments arranged by size:
1847 746 440
BsiHKAI G_wGCw'C
Cuts at: 0 141 197 1302 1328 1832 2069 2432 2441
Size: 141 56 1105 26 504 237 363 9
Cuts at: 2441 2702 2756 3033
Size: 261 54 277
Fragments arranged by size:
1105 504 363 277 261 237 141 56
54 26 9
BsmI GAATG_Cn'
Cuts at: 0 975 3033
Size: 975 2058
Bsp1286I G_dGCh'C
Cuts at: 0 141 197 952 1302 1328 1637 1832 2028
Size: 141 56 755 350 26 309 195 196
Cuts at: 2028 2069 2309 2432 2441 2649 2702 2728 2756
Size: 41 240 123 9 208 53 26 28
Cuts at: 2756 3033
Size: 277
Fragments arranged by size:
755 350 309 277 240 208 196 195
141 123 56 53 41 28 26 26
9
BspEI T'CCGG_A
Cuts at: 0 1288 1683 2779 3033
Size: 1288 395 1096 254
Fragments arranged by size:
1288 1096 395 254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 699 1218 2693 2711 3033
Size: 206 493 519 1475 18 322
Fragments arranged by size:
1475 519 493 322 206 18
BsrBI CCG'CTC
Cuts at: 0 93 1506 2390 3033
Size: 93 1413 884 643
Fragments arranged by size:
1413 884 643 93
BsrDI GCAATG_nn'
Cuts at: 0 558 2247 3033
Size: 558 1689 786
Fragments arranged by size:
1689 786 558
BsrFI r'CCGG_y
Cuts at: 0 133 283 911 1165 1185 1584 2567 3033
Size: 133 150 628 254 20 399 983 466
Fragments arranged by size:
983 628 466 399 254 150 133 20
BssHII G'CGCG_C
Cuts at: 0 1384 1522 3033
Size: 1384 138 1511
Fragments arranged by size:
1511 1384 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 1899 2064 3033
Size: 201 1698 165 969
Fragments arranged by size:
1698 969 201 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 873 1644 2868 3033
Size: 873 771 1224 165
Fragments arranged by size:
1224 873 771 165
BstYI r'GATC_y
Cuts at: 0 330 1795 2030 2252 3033
Size: 330 1465 235 222 781
Fragments arranged by size:
1465 781 330 235 222
Bsu36I CC'TnA_GG
Cuts at: 0 1968 2600 3033
Size: 1968 632 433
Fragments arranged by size:
1968 632 433
BtgI C'CryG_G
Cuts at: 0 239 746 944 2309 2981 3033
Size: 239 507 198 1365 672 52
Fragments arranged by size:
1365 672 507 239 198 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1330 1655 3033
Size: 1330 325 1378
Fragments arranged by size:
1378 1330 325
BtsI GCAGTG_nn'
Cuts at: 0 899 1047 1705 3033
Size: 899 148 658 1328
Fragments arranged by size:
1328 899 658 148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 658 693 3033
Size: 658 35 2340
Fragments arranged by size:
2340 658 35
DraIII CAC_nnn'GTG
Cuts at: 0 936 2040 2529 3033
Size: 936 1104 489 504
Fragments arranged by size:
1104 936 504 489
EaeI y'GGCC_r
Cuts at: 0 85 594 896 1183 1265 1370 2244 2714
Size: 85 509 302 287 82 105 874 470
Cuts at: 2714 2870 2960 3033
Size: 156 90 73
Fragments arranged by size:
874 509 470 302 287 156 105 90
85 82 73
EagI C'GGCC_G
Cuts at: 0 1183 3033
Size: 1183 1850
EarI CTCTTCn'nnn_
Cuts at: 0 760 3033
Size: 760 2273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1572 2593 3033
Size: 1572 1021 440
Fragments arranged by size:
1572 1021 440
EcoICRI GAG'CTC
Cuts at: 0 2430 3033
Size: 2430 603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 556 718 754 1264 1312 1707 1968
Size: 463 93 162 36 510 48 395 261
Cuts at: 1968 2149 2230 2278 2446 2473 2880 3033
Size: 181 81 48 168 27 407 153
Fragments arranged by size:
510 463 407 395 261 181 168 162
153 93 81 48 48 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1309 1856 3033
Size: 1309 547 1177
Fragments arranged by size:
1309 1177 547
EcoO109I rG'GnC_Cy
Cuts at: 0 58 840 1276 1408 1614 2589 2682 2724
Size: 58 782 436 132 206 975 93 42
Cuts at: 2724 2725 2820 2923 3033
Size: 1 95 103 110
Fragments arranged by size:
975 782 436 206 132 110 103 95
93 58 42 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2067 2241 3033
Size: 2067 174 792
Fragments arranged by size:
2067 792 174
FseI GG_CCGG'CC
Cuts at: 0 1189 3033
Size: 1189 1844
FspI TGC'GCA
Cuts at: 0 626 1022 2677 3033
Size: 626 396 1655 356
Fragments arranged by size:
1655 626 396 356
HaeII r_GCGC'y
Cuts at: 0 8 441 660 1209 1252 2523 2630 2798
Size: 8 433 219 549 43 1271 107 168
Cuts at: 2798 3033
Size: 235
Fragments arranged by size:
1271 549 433 235 219 168 107 43
8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1951 1983 2482 2514 2555 2587 2812 2844
Size: 1951 32 499 32 41 32 225 32
Cuts at: 2844 3033
Size: 189
Fragments arranged by size:
1951 499 225 189 41 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2334 3033
Size: 2334 699
KasI G'GCGC_C
Cuts at: 0 4 437 656 2519 2626 3033
Size: 4 433 219 1863 107 407
Fragments arranged by size:
1863 433 407 219 107 4
MscI TGG'CCA
Cuts at: 0 898 1372 2716 2872 3033
Size: 898 474 1344 156 161
Fragments arranged by size:
1344 898 474 161 156
MslI CAynn'nnrTG
Cuts at: 0 212 284 352 494 763 937 2866 3033
Size: 212 72 68 142 269 174 1929 167
Fragments arranged by size:
1929 269 212 174 167 142 72 68
MspA1I CmG'CkG
Cuts at: 0 748 831 1011 1242 1349 2007 2021 2121
Size: 748 83 180 231 107 658 14 100
Cuts at: 2121 2208 2370 2720 2952 3033
Size: 87 162 350 232 81
Fragments arranged by size:
748 658 350 232 231 180 162 107
100 87 83 81 14
NaeI GCC'GGC
Cuts at: 0 1187 3033
Size: 1187 1846
NarI GG'CG_CC
Cuts at: 0 5 438 657 2520 2627 3033
Size: 5 433 219 1863 107 406
Fragments arranged by size:
1863 433 406 219 107 5
NcoI C'CATG_G
Cuts at: 0 944 2981 3033
Size: 944 2037 52
Fragments arranged by size:
2037 944 52
NgoMIV G'CCGG_C
Cuts at: 0 1185 3033
Size: 1185 1848
NspI r_CATG'y
Cuts at: 0 164 186 981 2367 3033
Size: 164 22 795 1386 666
Fragments arranged by size:
1386 795 666 164 22
PasI CC'CwG_GG
Cuts at: 0 906 1551 1716 2394 3033
Size: 906 645 165 678 639
Fragments arranged by size:
906 678 645 639 165
PciI A'CATG_T
Cuts at: 0 977 3033
Size: 977 2056
PflMI CCAn_nnn'nTGG
Cuts at: 0 980 1368 3033
Size: 980 388 1665
Fragments arranged by size:
1665 980 388
PfoI T'CCnGG_A
Cuts at: 0 299 2255 3033
Size: 299 1956 778
Fragments arranged by size:
1956 778 299
PmlI CAC'GTG
Cuts at: 0 1368 3033
Size: 1368 1665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1678 1710 3033
Size: 1678 32 1323
Fragments arranged by size:
1678 1323 32
PpuMI rG'GwC_Cy
Cuts at: 0 840 1276 1614 2589 2682 2820 3033
Size: 840 436 338 975 93 138 213
Fragments arranged by size:
975 840 436 338 213 138 93
PsiI TTA'TAA
Cuts at: 0 1544 3033
Size: 1544 1489
PspOMI G'GGCC_C
Cuts at: 0 2724 3033
Size: 2724 309
PstI C_TGCA'G
Cuts at: 0 1229 2556 2855 3033
Size: 1229 1327 299 178
Fragments arranged by size:
1327 1229 299 178
PvuII CAG'CTG
Cuts at: 0 1011 1242 2370 2720 2952 3033
Size: 1011 231 1128 350 232 81
Fragments arranged by size:
1128 1011 350 232 231 81
SacI G_AGCT'C
Cuts at: 0 2432 3033
Size: 2432 601
SacII CC_GC'GG
Cuts at: 0 749 3033
Size: 749 2284
SanDI GG'GwC_CC
Cuts at: 0 1276 3033
Size: 1276 1757
SbfI CC_TGCA'GG
Cuts at: 0 1229 2855 3033
Size: 1229 1626 178
Fragments arranged by size:
1626 1229 178
ScaI AGT'ACT
Cuts at: 0 250 3033
Size: 250 2783
SexAI A'CCwGG_T
Cuts at: 0 1105 2036 2525 3033
Size: 1105 931 489 508
Fragments arranged by size:
1105 931 508 489
SfcI C'TryA_G
Cuts at: 0 1225 2552 2851 3033
Size: 1225 1327 299 182
Fragments arranged by size:
1327 1225 299 182
SfoI GGC'GCC
Cuts at: 0 6 439 658 2521 2628 3033
Size: 6 433 219 1863 107 405
Fragments arranged by size:
1863 433 405 219 107 6
SgrAI Cr'CCGG_yG
Cuts at: 0 283 3033
Size: 283 2750
SmaI CCC'GGG
Cuts at: 0 2618 3033
Size: 2618 415
SmlI C'TyrA_G
Cuts at: 0 2814 2902 3033
Size: 2814 88 131
Fragments arranged by size:
2814 131 88
SspI AAT'ATT
Cuts at: 0 1652 3033
Size: 1652 1381
StuI AGG'CCT
Cuts at: 0 613 1966 3033
Size: 613 1353 1067
Fragments arranged by size:
1353 1067 613
StyI C'CwwG_G
Cuts at: 0 790 866 944 2051 2981 3033
Size: 790 76 78 1107 930 52
Fragments arranged by size:
1107 930 790 78 76 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 453 502 2499 3033
Size: 297 156 49 1997 534
Fragments arranged by size:
1997 534 297 156 49
TatI w'GTAC_w
Cuts at: 0 248 293 857 3033
Size: 248 45 564 2176
Fragments arranged by size:
2176 564 248 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 697 729 799 831 1769 1801 2122 2154
Size: 697 32 70 32 938 32 321 32
Cuts at: 2154 3033
Size: 879
Fragments arranged by size:
938 879 697 321 70 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 69 307 1342 3033
Size: 69 238 1035 1691
Fragments arranged by size:
1691 1035 238 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 253 517 2970 3033
Size: 253 264 2453 63
Fragments arranged by size:
2453 264 253 63
XmaI C'CCGG_G
Cuts at: 0 2616 3033
Size: 2616 417
Enzymes that do cut:
AarI AclI AcuI AflIII AgeI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI FspI HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PstI
PvuII SacI SacII SanDI SbfI ScaI SexAI SfcI
SfoI SgrAI SmaI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AhdI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BclI BfrBI BglII
BmrI BmtI BsiWI BsmBI BspHI BsrGI BssSI BstBI
BstEII BstZ17I ClaI DraI DrdI EcoRI EcoRV FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PmeI PshAI PspXI PsrI
PvuI RsrII SalI SapI SfiI SgfI SnaBI SpeI
SphI SrfI SwaI XbaI XhoI XmnI ZraI