Restriction map for
pF1KB5676
(Linear) (Six-base) MAPSORT of: KIBB5676.seq Check: 3664 from: 1 to: 2418
KIBB5676.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 398 2384 2418
Size: 398 1986 34
Fragments arranged by size:
1986 398 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1531 2269 2295 2306 2418
Size: 1126 405 738 26 11 112
Fragments arranged by size:
1126 738 405 112 26 11
AflII C'TTAA_G
Cuts at: 0 403 2418
Size: 403 2015
AflIII A'CryG_T
Cuts at: 0 1017 2418
Size: 1017 1401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1238 1257 2418
Size: 1238 19 1161
Fragments arranged by size:
1238 1161 19
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2002 2034 2418
Size: 2002 32 384
Fragments arranged by size:
2002 384 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1252 1473 1812 2185 2418
Size: 1252 221 339 373 233
Fragments arranged by size:
1252 373 339 233 221
ApoI r'AATT_y
Cuts at: 0 1109 1504 2418
Size: 1109 395 914
Fragments arranged by size:
1109 914 395
AvaI C'yCGr_G
Cuts at: 0 764 2418
Size: 764 1654
AvrII C'CTAG_G
Cuts at: 0 285 2418
Size: 285 2133
BanI G'GyrC_C
Cuts at: 0 1410 2418
Size: 1410 1008
BbsI GAAGACnn'nnnn_
Cuts at: 0 1436 2399 2418
Size: 1436 963 19
Fragments arranged by size:
1436 963 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 349 1493 2418
Size: 349 1144 925
Fragments arranged by size:
1144 925 349
BclI T'GATC_A
Cuts at: 0 803 1917 2418
Size: 803 1114 501
Fragments arranged by size:
1114 803 501
Bme1580I G_kGCm'C
Cuts at: 0 1017 2418
Size: 1017 1401
BmrI ACTGGGnnnn_n'
Cuts at: 0 1987 2342 2418
Size: 1987 355 76
Fragments arranged by size:
1987 355 76
BmtI G_CTAG'C
Cuts at: 0 640 2418
Size: 640 1778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 515 806 2069 2418
Size: 29 486 291 1263 349
Fragments arranged by size:
1263 486 349 291 29
Bpu10I CC'TnA_GC
Cuts at: 0 1634 2418
Size: 1634 784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1386 1954 2418
Size: 1386 568 464
Fragments arranged by size:
1386 568 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 1297 2418
Size: 1297 1121
BsaWI w'CCGG_w
Cuts at: 0 437 1355 2418
Size: 437 918 1063
Fragments arranged by size:
1063 918 437
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1603 1633 2418
Size: 1603 30 785
Fragments arranged by size:
1603 785 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 2418
Size: 886 1532
BseYI C'CCAG_C
Cuts at: 0 125 1062 2418
Size: 125 937 1356
Fragments arranged by size:
1356 937 125
BsiHKAI G_wGCw'C
Cuts at: 0 1246 1496 2418
Size: 1246 250 922
Fragments arranged by size:
1246 922 250
BsmI GAATG_Cn'
Cuts at: 0 891 1606 2418
Size: 891 715 812
Fragments arranged by size:
891 812 715
BsmBI CGTCTCn'nnnn_
Cuts at: 0 183 2418
Size: 183 2235
Bsp1286I G_dGCh'C
Cuts at: 0 1017 1246 1496 2418
Size: 1017 229 250 922
Fragments arranged by size:
1017 922 250 229
BspEI T'CCGG_A
Cuts at: 0 437 2418
Size: 437 1981
BspHI T'CATG_A
Cuts at: 0 821 1322 2418
Size: 821 501 1096
Fragments arranged by size:
1096 821 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1988 2418
Size: 1988 430
BsrBI CCG'CTC
Cuts at: 0 963 2418
Size: 963 1455
BsrDI GCAATG_nn'
Cuts at: 0 1589 2418
Size: 1589 829
BsrFI r'CCGG_y
Cuts at: 0 1871 2418
Size: 1871 547
BssHII G'CGCG_C
Cuts at: 0 2227 2418
Size: 2227 191
BssSI C'ACGA_G
Cuts at: 0 1754 2418
Size: 1754 664
BstEII G'GTnAC_C
Cuts at: 0 1419 2418
Size: 1419 999
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2007 2418
Size: 2007 411
BstYI r'GATC_y
Cuts at: 0 504 1145 1281 1808 2189 2418
Size: 504 641 136 527 381 229
Fragments arranged by size:
641 527 504 381 229 136
BstZ17I GTA'TAC
Cuts at: 0 399 2385 2418
Size: 399 1986 33
Fragments arranged by size:
1986 399 33
BtgI C'CryG_G
Cuts at: 0 462 1400 2156 2418
Size: 462 938 756 262
Fragments arranged by size:
938 756 462 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 909 2418
Size: 909 1509
BtsI GCAGTG_nn'
Cuts at: 0 546 1387 2364 2385 2418
Size: 546 841 977 21 33
Fragments arranged by size:
977 841 546 33 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1737 1772 2418
Size: 1737 35 646
Fragments arranged by size:
1737 646 35
DraIII CAC_nnn'GTG
Cuts at: 0 1649 2418
Size: 1649 769
DrdI GACnn_nn'nnGTC
Cuts at: 0 1910 2418
Size: 1910 508
EaeI y'GGCC_r
Cuts at: 0 1580 1908 2090 2418
Size: 1580 328 182 328
Fragments arranged by size:
1580 328 328 182
EarI CTCTTCn'nnn_
Cuts at: 0 181 394 578 2162 2418
Size: 181 213 184 1584 256
Fragments arranged by size:
1584 256 213 184 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 515 806 1126 1531 2069 2269 2295
Size: 29 486 291 320 405 538 200 26
Cuts at: 2295 2306 2418
Size: 11 112
Fragments arranged by size:
538 486 405 320 291 200 112 29
26 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 713 743 1189 2418
Size: 688 25 30 446 1229
Fragments arranged by size:
1229 688 446 30 25
EcoO109I rG'GnC_Cy
Cuts at: 0 1440 1630 2418
Size: 1440 190 788
Fragments arranged by size:
1440 788 190
EcoRI G'AATT_C
Cuts at: 0 1109 2418
Size: 1109 1309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 2418
Size: 429 1989
FspI TGC'GCA
Cuts at: 0 1984 2418
Size: 1984 434
HaeII r_GCGC'y
Cuts at: 0 1865 2418
Size: 1865 553
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 571 603 795 827 1816 1848
Size: 19 32 520 32 192 32 989 32
Cuts at: 1848 1936 1968 2418
Size: 88 32 450
Fragments arranged by size:
989 520 450 192 88 32 32 32
32 32 19
HincII GTy'rAC
Cuts at: 0 986 2418
Size: 986 1432
MfeI C'AATT_G
Cuts at: 0 695 2418
Size: 695 1723
MscI TGG'CCA
Cuts at: 0 1582 2092 2418
Size: 1582 510 326
Fragments arranged by size:
1582 510 326
MslI CAynn'nnrTG
Cuts at: 0 1214 1220 2418
Size: 1214 6 1198
Fragments arranged by size:
1214 1198 6
NcoI C'CATG_G
Cuts at: 0 2156 2418
Size: 2156 262
NheI G'CTAG_C
Cuts at: 0 636 2418
Size: 636 1782
NspI r_CATG'y
Cuts at: 0 1021 1219 1833 2418
Size: 1021 198 614 585
Fragments arranged by size:
1021 614 585 198
PciI A'CATG_T
Cuts at: 0 1017 2418
Size: 1017 1401
PfoI T'CCnGG_A
Cuts at: 0 416 738 2418
Size: 416 322 1680
Fragments arranged by size:
1680 416 322
PpuMI rG'GwC_Cy
Cuts at: 0 1630 2418
Size: 1630 788
PspXI vC'TCGA_Gb
Cuts at: 0 764 2418
Size: 764 1654
PstI C_TGCA'G
Cuts at: 0 628 2418
Size: 628 1790
SfcI C'TryA_G
Cuts at: 0 624 770 1121 1465 2418
Size: 624 146 351 344 953
Fragments arranged by size:
953 624 351 344 146
SmlI C'TyrA_G
Cuts at: 0 403 764 1365 1969 2418
Size: 403 361 601 604 449
Fragments arranged by size:
604 601 449 403 361
StyI C'CwwG_G
Cuts at: 0 285 1532 1748 2000 2156 2418
Size: 285 1247 216 252 156 262
Fragments arranged by size:
1247 285 262 252 216 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1210 1511 1808 2418
Size: 1210 301 297 610
Fragments arranged by size:
1210 610 301 297
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2319 2351 2418
Size: 2319 32 67
Fragments arranged by size:
2319 67 32
Tth111I GACn'n_nGTC
Cuts at: 0 1795 2418
Size: 1795 623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1421 2338 2418
Size: 1421 917 80
Fragments arranged by size:
1421 917 80
XhoI C'TCGA_G
Cuts at: 0 764 2418
Size: 764 1654
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AloI AlwNI ApoI
AvaI AvrII BanI BbsI BciVI BclI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII MfeI MscI MslI NcoI NheI NspI
PciI PfoI PpuMI PspXI PstI SfcI SmlI StyI
TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanII
BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI
BplI BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsrGI
BstAPI BstBI Bsu36I ClaI DraI EagI EciI EcoICRI
EcoRV FseI FspAI HindIII HpaI KasI KpnI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI XbaI XmaI XmnI ZraI