(Linear) (Six-base) MAPSORT of: KIBB5677.seq  Check: 1566  from: 1  to: 1011



KIBB5677.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0     250    1011 
   Size:        250     761
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     386    1011 
   Size:         29     357     625

  Fragments arranged by size: 

                 625     357      29
 
ApoI r'AATT_y

Cuts at:      0      88     130     326     438     593     724     937    1011 
   Size:         88      42     196     112     155     131     213      74

  Fragments arranged by size: 

                 213     196     155     131     112      88      74      42
            
 
AseI AT'TA_AT

Cuts at:      0     800    1011 
   Size:        800     211
 
BanI G'GyrC_C

Cuts at:      0     250     631    1011 
   Size:        250     381     380

  Fragments arranged by size: 

                 381     380     250
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     345    1011 
   Size:        345     666
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     265     299    1011 
   Size:        265      34     712

  Fragments arranged by size: 

                 712     265      34
 
BclI T'GATC_A

Cuts at:      0     192    1011 
   Size:        192     819
 
BglII A'GATC_T

Cuts at:      0     580    1011 
   Size:        580     431
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458    1011 
   Size:        458     553
 
BsaBI GATnn'nnATC

Cuts at:      0     386    1011 
   Size:        386     625
 
BsaWI w'CCGG_w

Cuts at:      0      96     257    1011 
   Size:         96     161     754

  Fragments arranged by size: 

                 754     161      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     196     226    1011 
   Size:        196      30     785

  Fragments arranged by size: 

                 785     196      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     104     236     882    1011 
   Size:        104     132     646     129

  Fragments arranged by size: 

                 646     132     129     104
 
BspEI T'CCGG_A

Cuts at:      0      96    1011 
   Size:         96     915
 
BspHI T'CATG_A

Cuts at:      0     483    1011 
   Size:        483     528
 
BstEII G'GTnAC_C

Cuts at:      0     922    1011 
   Size:        922      89
 
BstYI r'GATC_y

Cuts at:      0     468     580    1011 
   Size:        468     112     431

  Fragments arranged by size: 

                 468     431     112
 
Bsu36I CC'TnA_GG

Cuts at:      0     116    1011 
   Size:        116     895
 
DraI TTT'AAA

Cuts at:      0     135     729    1011 
   Size:        135     594     282

  Fragments arranged by size: 

                 594     282     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     500    1011 
   Size:        500     511
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     636    1011 
   Size:        636     375
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     386     458    1011 
   Size:         29     357      72     553

  Fragments arranged by size: 

                 553     357      72      29
 
EcoRI G'AATT_C

Cuts at:      0     438    1011 
   Size:        438     573
 
EcoRV GAT'ATC

Cuts at:      0     424    1011 
   Size:        424     587
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1003    1011 
   Size:       1003       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     949     981    1011 
   Size:        949      32      30

  Fragments arranged by size: 

                 949      32      30
 
HindIII A'AGCT_T

Cuts at:      0     149    1011 
   Size:        149     862
 
KpnI G_GTAC'C

Cuts at:      0     254    1011 
   Size:        254     757
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     338    1011 
   Size:        338     673
 
MslI CAynn'nnrTG

Cuts at:      0     832    1011 
   Size:        832     179
 
ScaI AGT'ACT

Cuts at:      0     986    1011 
   Size:        986      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     337    1011 
   Size:        337     674
 
TatI w'GTAC_w

Cuts at:      0     984    1011 
   Size:        984      27
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI     AseI     BanI     BbsI     BcgI     BclI
    BglII     BpmI    BsaBI    BsaWI    BsaXI    BseRI    BspEI    BspHI
   BstEII    BstYI   Bsu36I     DraI     EarI     EciI  Eco57MI    EcoRI
    EcoRV     FalI    Hin4I  HindIII     KpnI     MmeI     MslI     ScaI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI