Restriction map for
pF1KB5679
(Linear) (Six-base) MAPSORT of: KIBB5679.seq Check: 8907 from: 1 to: 2880
KIBB5679.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 0 1042 2595 2880
Size: 1042 1553 285
Fragments arranged by size:
1553 1042 285
Acc65I G'GTAC_C
Cuts at: 0 1748 2880
Size: 1748 1132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 931 2164 2533 2880
Size: 349 582 1233 369 347
Fragments arranged by size:
1233 582 369 349 347
AfeI AGC'GCT
Cuts at: 0 1693 2880
Size: 1693 1187
AflII C'TTAA_G
Cuts at: 0 1171 2880
Size: 1171 1709
ApaI G_GGCC'C
Cuts at: 0 1737 2880
Size: 1737 1143
ApoI r'AATT_y
Cuts at: 0 575 1290 1417 1864 2468 2656 2880
Size: 575 715 127 447 604 188 224
Fragments arranged by size:
715 604 575 447 224 188 127
BamHI G'GATC_C
Cuts at: 0 144 1548 2880
Size: 144 1404 1332
Fragments arranged by size:
1404 1332 144
BanI G'GyrC_C
Cuts at: 0 1748 2880
Size: 1748 1132
BanII G_rGCy'C
Cuts at: 0 1737 2230 2671 2880
Size: 1737 493 441 209
Fragments arranged by size:
1737 493 441 209
BbsI GAAGACnn'nnnn_
Cuts at: 0 1724 2880
Size: 1724 1156
BclI T'GATC_A
Cuts at: 0 2687 2880
Size: 2687 193
BfrBI ATG'CAT
Cuts at: 0 98 862 2880
Size: 98 764 2018
Fragments arranged by size:
2018 764 98
BglII A'GATC_T
Cuts at: 0 339 2880
Size: 339 2541
Bme1580I G_kGCm'C
Cuts at: 0 1737 2880
Size: 1737 1143
BmrI ACTGGGnnnn_n'
Cuts at: 0 154 203 309 2319 2880
Size: 154 49 106 2010 561
Fragments arranged by size:
2010 561 154 106 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1612 1684 2235 2854 2880
Size: 1019 593 72 551 619 26
Fragments arranged by size:
1019 619 593 551 72 26
Bpu10I CC'TnA_GC
Cuts at: 0 199 2880
Size: 199 2681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 2149 2753 2782 2880
Size: 1378 771 604 29 98
Fragments arranged by size:
1378 771 604 98 29
BsaBI GATnn'nnATC
Cuts at: 0 2689 2880
Size: 2689 191
BsaHI Gr'CG_yC
Cuts at: 0 1039 2592 2880
Size: 1039 1553 288
Fragments arranged by size:
1553 1039 288
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1426 2880
Size: 1426 1454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2033 2880
Size: 2033 847
BsiHKAI G_wGCw'C
Cuts at: 0 2397 2880
Size: 2397 483
BsmI GAATG_Cn'
Cuts at: 0 360 2880
Size: 360 2520
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1030 2880
Size: 1030 1850
Bsp1286I G_dGCh'C
Cuts at: 0 1737 2230 2397 2671 2880
Size: 1737 493 167 274 209
Fragments arranged by size:
1737 493 274 209 167
BspHI T'CATG_A
Cuts at: 0 432 1989 2880
Size: 432 1557 891
Fragments arranged by size:
1557 891 432
BsrBI CCG'CTC
Cuts at: 0 2252 2880
Size: 2252 628
BsrDI GCAATG_nn'
Cuts at: 0 23 312 2880
Size: 23 289 2568
Fragments arranged by size:
2568 289 23
BssSI C'ACGA_G
Cuts at: 0 1779 2880
Size: 1779 1101
BstEII G'GTnAC_C
Cuts at: 0 1089 2880
Size: 1089 1791
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1129 2880
Size: 1129 1751
BstYI r'GATC_y
Cuts at: 0 144 339 1548 1836 2880
Size: 144 195 1209 288 1044
Fragments arranged by size:
1209 1044 288 195 144
Bsu36I CC'TnA_GG
Cuts at: 0 1967 2880
Size: 1967 913
BtgI C'CryG_G
Cuts at: 0 1004 2880
Size: 1004 1876
BtsI GCAGTG_nn'
Cuts at: 0 445 1042 2880
Size: 445 597 1838
Fragments arranged by size:
1838 597 445
DraI TTT'AAA
Cuts at: 0 2372 2880
Size: 2372 508
DrdI GACnn_nn'nnGTC
Cuts at: 0 241 2880
Size: 241 2639
EarI CTCTTCn'nnn_
Cuts at: 0 669 1071 2323 2880
Size: 669 402 1252 557
Fragments arranged by size:
1252 669 557 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 931 1019 1612 1684 2164 2235 2533
Size: 349 582 88 593 72 480 71 298
Cuts at: 2533 2854 2880
Size: 321 26
Fragments arranged by size:
593 582 480 349 321 298 88 72
71 26
EcoO109I rG'GnC_Cy
Cuts at: 0 1017 1733 1734 2880
Size: 1017 716 1 1146
Fragments arranged by size:
1146 1017 716 1
EcoRI G'AATT_C
Cuts at: 0 575 1290 2880
Size: 575 715 1590
Fragments arranged by size:
1590 715 575
EcoRV GAT'ATC
Cuts at: 0 945 2151 2880
Size: 945 1206 729
Fragments arranged by size:
1206 945 729
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 757 2880
Size: 757 2123
HaeII r_GCGC'y
Cuts at: 0 1695 2880
Size: 1695 1185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 799 831 1510 1542 1815 1847 2212 2244
Size: 799 32 679 32 273 32 365 32
Cuts at: 2244 2481 2513 2653 2685 2880
Size: 237 32 140 32 195
Fragments arranged by size:
799 679 365 273 237 195 140 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2486 2880
Size: 2486 394
KpnI G_GTAC'C
Cuts at: 0 1752 2880
Size: 1752 1128
MfeI C'AATT_G
Cuts at: 0 722 2880
Size: 722 2158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1701 2085 2872 2880
Size: 725 976 384 787 8
Fragments arranged by size:
976 787 725 384 8
MslI CAynn'nnrTG
Cuts at: 0 2683 2880
Size: 2683 197
MspA1I CmG'CkG
Cuts at: 0 1318 1938 2880
Size: 1318 620 942
Fragments arranged by size:
1318 942 620
NcoI C'CATG_G
Cuts at: 0 1004 2880
Size: 1004 1876
NdeI CA'TA_TG
Cuts at: 0 2673 2880
Size: 2673 207
NsiI A_TGCA'T
Cuts at: 0 100 864 2880
Size: 100 764 2016
Fragments arranged by size:
2016 764 100
NspI r_CATG'y
Cuts at: 0 708 1572 2880
Size: 708 864 1308
Fragments arranged by size:
1308 864 708
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 2127 2331 2880
Size: 1113 1014 204 549
Fragments arranged by size:
1113 1014 549 204
PfoI T'CCnGG_A
Cuts at: 0 1712 2880
Size: 1712 1168
PsiI TTA'TAA
Cuts at: 0 539 2880
Size: 539 2341
PspOMI G'GGCC_C
Cuts at: 0 1733 2880
Size: 1733 1147
PstI C_TGCA'G
Cuts at: 0 1742 2880
Size: 1742 1138
PvuII CAG'CTG
Cuts at: 0 1938 2880
Size: 1938 942
SbfI CC_TGCA'GG
Cuts at: 0 1742 2880
Size: 1742 1138
ScaI AGT'ACT
Cuts at: 0 937 1441 2880
Size: 937 504 1439
Fragments arranged by size:
1439 937 504
SexAI A'CCwGG_T
Cuts at: 0 1136 2880
Size: 1136 1744
SfcI C'TryA_G
Cuts at: 0 1458 1738 2880
Size: 1458 280 1142
Fragments arranged by size:
1458 1142 280
SmlI C'TyrA_G
Cuts at: 0 1171 1393 2164 2761 2768 2880
Size: 1171 222 771 597 7 112
Fragments arranged by size:
1171 771 597 222 112 7
SspI AAT'ATT
Cuts at: 0 2142 2880
Size: 2142 738
StyI C'CwwG_G
Cuts at: 0 187 1004 2720 2880
Size: 187 817 1716 160
Fragments arranged by size:
1716 817 187 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1396 1848 2380 2880
Size: 1396 452 532 500
Fragments arranged by size:
1396 532 500 452
TatI w'GTAC_w
Cuts at: 0 778 935 1439 2880
Size: 778 157 504 1441
Fragments arranged by size:
1441 778 504 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 2701 2733 2880
Size: 129 32 2540 32 147
Fragments arranged by size:
2540 147 129 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 820 2880
Size: 820 2060
XmnI GAAnn'nnTTC
Cuts at: 0 182 2880
Size: 182 2698
ZraI GAC'GTC
Cuts at: 0 1040 2593 2880
Size: 1040 1553 287
Fragments arranged by size:
1553 1040 287
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflII ApaI ApoI BamHI
BanI BanII BbsI BclI BfrBI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaBI BsaHI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BssSI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EarI
Eco57MI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
KpnI MfeI MmeI MslI MspA1I NcoI NdeI NsiI
NspI PflMI PfoI PsiI PspOMI PstI PvuII SbfI
ScaI SexAI SfcI SmlI SspI StyI TaqII TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BcgI BciVI BglI BlpI BmgBI
BmtI BplI BsaI BsaAI BsaWI BsaXI BseYI BsiEI
BsiWI BspEI BspMI BsrFI BsrGI BssHII BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraIII EaeI EagI EciI
EcoICRI EcoNI FseI FspI FspAI HindIII HpaI KasI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI