Restriction map for
pF1KB5682
(Linear) (Six-base) MAPSORT of: KIBB5682.seq Check: 777 from: 1 to: 1656
KIBB5682.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 681 1656
Size: 681 975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 229 542 847 1656
Size: 160 69 313 305 809
Fragments arranged by size:
809 313 305 160 69
AflIII A'CryG_T
Cuts at: 0 330 1656
Size: 330 1326
AhdI GACnn_n'nnGTC
Cuts at: 0 9 1656
Size: 9 1647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 467 1174 1656
Size: 467 707 482
Fragments arranged by size:
707 482 467
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 1656
Size: 1259 32 365
Fragments arranged by size:
1259 365 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1375 1459 1656
Size: 1375 84 197
Fragments arranged by size:
1375 197 84
ApaI G_GGCC'C
Cuts at: 0 1596 1656
Size: 1596 60
ApaLI G'TGCA_C
Cuts at: 0 1259 1656
Size: 1259 397
ApoI r'AATT_y
Cuts at: 0 1577 1656
Size: 1577 79
BanI G'GyrC_C
Cuts at: 0 1199 1656
Size: 1199 457
BanII G_rGCy'C
Cuts at: 0 198 1596 1656
Size: 198 1398 60
Fragments arranged by size:
1398 198 60
BbvCI CC'TCA_GC
Cuts at: 0 731 1656
Size: 731 925
BciVI GTATCCnnnnn_n'
Cuts at: 0 1048 1656
Size: 1048 608
BfrBI ATG'CAT
Cuts at: 0 1496 1656
Size: 1496 160
BglII A'GATC_T
Cuts at: 0 1283 1656
Size: 1283 373
Bme1580I G_kGCm'C
Cuts at: 0 1263 1596 1656
Size: 1263 333 60
Fragments arranged by size:
1263 333 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 694 1656
Size: 694 962
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 470 1656
Size: 470 1186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1091 1286 1421 1582 1656
Size: 315 776 195 135 161 74
Fragments arranged by size:
776 315 195 161 135 74
Bpu10I CC'TnA_GC
Cuts at: 0 731 1656
Size: 731 925
BsaAI yAC'GTr
Cuts at: 0 1059 1656
Size: 1059 597
BsaWI w'CCGG_w
Cuts at: 0 1530 1656
Size: 1530 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1259 1289 1656
Size: 1259 30 367
Fragments arranged by size:
1259 367 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1088 1656
Size: 1088 568
BseYI C'CCAG_C
Cuts at: 0 591 1252 1523 1656
Size: 591 661 271 133
Fragments arranged by size:
661 591 271 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 803 1180 1343 1656
Size: 803 377 163 313
Fragments arranged by size:
803 377 313 163
BsiHKAI G_wGCw'C
Cuts at: 0 198 1263 1656
Size: 198 1065 393
Fragments arranged by size:
1065 393 198
BsmI GAATG_Cn'
Cuts at: 0 925 1656
Size: 925 731
BsmBI CGTCTCn'nnnn_
Cuts at: 0 295 1656
Size: 295 1361
Bsp1286I G_dGCh'C
Cuts at: 0 198 1263 1596 1656
Size: 198 1065 333 60
Fragments arranged by size:
1065 333 198 60
BsrDI GCAATG_nn'
Cuts at: 0 974 1656
Size: 974 682
BsrGI T'GTAC_A
Cuts at: 0 1224 1656
Size: 1224 432
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1366 1375 1656
Size: 1366 9 281
Fragments arranged by size:
1366 281 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1656
Size: 684 972
BstYI r'GATC_y
Cuts at: 0 1283 1656
Size: 1283 373
BstZ17I GTA'TAC
Cuts at: 0 682 1656
Size: 682 974
BtgI C'CryG_G
Cuts at: 0 1391 1656
Size: 1391 265
BtsI GCAGTG_nn'
Cuts at: 0 439 629 1656
Size: 439 190 1027
Fragments arranged by size:
1027 439 190
DrdI GACnn_nn'nnGTC
Cuts at: 0 1216 1656
Size: 1216 440
EaeI y'GGCC_r
Cuts at: 0 855 1656
Size: 855 801
EcoICRI GAG'CTC
Cuts at: 0 196 1656
Size: 196 1460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 229 315 542 847 1091 1286 1421
Size: 160 69 86 227 305 244 195 135
Cuts at: 1421 1582 1656
Size: 161 74
Fragments arranged by size:
305 244 227 195 161 160 135 86
74 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 734 1070 1412 1656
Size: 734 336 342 244
Fragments arranged by size:
734 342 336 244
EcoO109I rG'GnC_Cy
Cuts at: 0 727 1593 1656
Size: 727 866 63
Fragments arranged by size:
866 727 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1535 1567 1656
Size: 27 1508 32 89
Fragments arranged by size:
1508 89 32 27
HincII GTy'rAC
Cuts at: 0 1096 1656
Size: 1096 560
MfeI C'AATT_G
Cuts at: 0 116 1656
Size: 116 1540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1145 1656
Size: 238 907 511
Fragments arranged by size:
907 511 238
MslI CAynn'nnrTG
Cuts at: 0 317 652 1656
Size: 317 335 1004
Fragments arranged by size:
1004 335 317
MspA1I CmG'CkG
Cuts at: 0 1375 1456 1656
Size: 1375 81 200
Fragments arranged by size:
1375 200 81
NsiI A_TGCA'T
Cuts at: 0 1498 1656
Size: 1498 158
NspI r_CATG'y
Cuts at: 0 334 1656
Size: 334 1322
PciI A'CATG_T
Cuts at: 0 330 1656
Size: 330 1326
PfoI T'CCnGG_A
Cuts at: 0 891 1656
Size: 891 765
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 1656
Size: 1259 32 365
Fragments arranged by size:
1259 365 32
PshAI GACnn'nnGTC
Cuts at: 0 1484 1656
Size: 1484 172
PspOMI G'GGCC_C
Cuts at: 0 1592 1656
Size: 1592 64
PstI C_TGCA'G
Cuts at: 0 446 1371 1656
Size: 446 925 285
Fragments arranged by size:
925 446 285
PvuII CAG'CTG
Cuts at: 0 1375 1456 1656
Size: 1375 81 200
Fragments arranged by size:
1375 200 81
SacI G_AGCT'C
Cuts at: 0 198 1656
Size: 198 1458
ScaI AGT'ACT
Cuts at: 0 1616 1656
Size: 1616 40
SfcI C'TryA_G
Cuts at: 0 442 1367 1656
Size: 442 925 289
Fragments arranged by size:
925 442 289
SspI AAT'ATT
Cuts at: 0 340 1656
Size: 340 1316
TatI w'GTAC_w
Cuts at: 0 1224 1614 1656
Size: 1224 390 42
Fragments arranged by size:
1224 390 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 82 651 1656
Size: 82 569 1005
Fragments arranged by size:
1005 569 82
XmnI GAAnn'nnTTC
Cuts at: 0 1616 1656
Size: 1616 40
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AloI AlwNI ApaI
ApaLI ApoI BanI BanII BbvCI BciVI BfrBI BglII
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI
BsrGI BstAPI BstXI BstYI BstZ17I BtgI BtsI DrdI
EaeI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MfeI
MmeI MslI MspA1I NsiI NspI PciI PfoI PpiI
PshAI PspOMI PstI PvuII SacI ScaI SfcI SspI
TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbsI BcgI BclI BglI BlpI BmgBI BmtI BpuEI
BsaI BsaBI BsaHI BsiEI BsiWI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstBI BstEII Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EarI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PflMI PmeI
PmlI PpuMI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI
ZraI