Restriction map for
pF1KB5685
(Linear) (Six-base) MAPSORT of: KIBB5685.seq Check: 1397 from: 1 to: 1206
KIBB5685.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 31 123 1206
Size: 31 92 1083
Fragments arranged by size:
1083 92 31
AccI GT'mk_AC
Cuts at: 0 920 1206
Size: 920 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 397 935 1206
Size: 393 4 538 271
Fragments arranged by size:
538 393 271 4
AflIII A'CryG_T
Cuts at: 0 959 1206
Size: 959 247
AlwNI CAG_nnn'CTG
Cuts at: 0 851 1029 1206
Size: 851 178 177
Fragments arranged by size:
851 178 177
ApaLI G'TGCA_C
Cuts at: 0 70 1206
Size: 70 1136
AvaI C'yCGr_G
Cuts at: 0 1114 1206
Size: 1114 92
BanI G'GyrC_C
Cuts at: 0 801 1206
Size: 801 405
BanII G_rGCy'C
Cuts at: 0 314 1206
Size: 314 892
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 601 1206
Size: 496 105 605
Fragments arranged by size:
605 496 105
BbvCI CC'TCA_GC
Cuts at: 0 766 1206
Size: 766 440
BclI T'GATC_A
Cuts at: 0 509 1206
Size: 509 697
BglI GCCn_nnn'nGGC
Cuts at: 0 100 1108 1206
Size: 100 1008 98
Fragments arranged by size:
1008 100 98
Bme1580I G_kGCm'C
Cuts at: 0 74 668 837 1206
Size: 74 594 169 369
Fragments arranged by size:
594 369 169 74
BmgBI CAC'GTC
Cuts at: 0 1017 1206
Size: 1017 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 1206
Size: 492 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 597 928 1206
Size: 190 407 331 278
Fragments arranged by size:
407 331 278 190
Bpu10I CC'TnA_GC
Cuts at: 0 766 1206
Size: 766 440
BsaAI yAC'GTr
Cuts at: 0 90 1206
Size: 90 1116
BsaWI w'CCGG_w
Cuts at: 0 419 1206
Size: 419 787
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 236 1065 1132 1206
Size: 236 829 67 74
Fragments arranged by size:
829 236 74 67
BseYI C'CCAG_C
Cuts at: 0 230 836 1206
Size: 230 606 370
Fragments arranged by size:
606 370 230
BsiHKAI G_wGCw'C
Cuts at: 0 74 314 427 1206
Size: 74 240 113 779
Fragments arranged by size:
779 240 113 74
BsmI GAATG_Cn'
Cuts at: 0 573 1206
Size: 573 633
Bsp1286I G_dGCh'C
Cuts at: 0 74 314 427 668 837 1206
Size: 74 240 113 241 169 369
Fragments arranged by size:
369 241 240 169 113 74
BspEI T'CCGG_A
Cuts at: 0 419 1206
Size: 419 787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 31 123 1206
Size: 31 92 1083
Fragments arranged by size:
1083 92 31
BsrDI GCAATG_nn'
Cuts at: 0 776 1206
Size: 776 430
BsrGI T'GTAC_A
Cuts at: 0 302 1206
Size: 302 904
BssSI C'ACGA_G
Cuts at: 0 987 1206
Size: 987 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1029 1206
Size: 1029 177
BstYI r'GATC_y
Cuts at: 0 372 1206
Size: 372 834
Bsu36I CC'TnA_GG
Cuts at: 0 925 1206
Size: 925 281
BtgI C'CryG_G
Cuts at: 0 322 536 753 1206
Size: 322 214 217 453
Fragments arranged by size:
453 322 217 214
EaeI y'GGCC_r
Cuts at: 0 197 585 720 1206
Size: 197 388 135 486
Fragments arranged by size:
486 388 197 135
EarI CTCTTCn'nnn_
Cuts at: 0 637 1206
Size: 637 569
EcoICRI GAG'CTC
Cuts at: 0 312 1206
Size: 312 894
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 393 397 597 928 935 1206
Size: 190 203 4 200 331 7 271
Fragments arranged by size:
331 271 203 200 190 7 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 618 1206
Size: 618 588
HaeII r_GCGC'y
Cuts at: 0 1030 1206
Size: 1030 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 458 490 1127 1159 1206
Size: 458 32 637 32 47
Fragments arranged by size:
637 458 47 32 32
HincII GTy'rAC
Cuts at: 0 921 1206
Size: 921 285
MscI TGG'CCA
Cuts at: 0 199 722 1206
Size: 199 523 484
Fragments arranged by size:
523 484 199
MspA1I CmG'CkG
Cuts at: 0 210 369 546 1087 1206
Size: 210 159 177 541 119
Fragments arranged by size:
541 210 177 159 119
NcoI C'CATG_G
Cuts at: 0 322 1206
Size: 322 884
NspI r_CATG'y
Cuts at: 0 774 963 1206
Size: 774 189 243
Fragments arranged by size:
774 243 189
PciI A'CATG_T
Cuts at: 0 959 1206
Size: 959 247
PflMI CCAn_nnn'nTGG
Cuts at: 0 1197 1206
Size: 1197 9
PfoI T'CCnGG_A
Cuts at: 0 737 1206
Size: 737 469
PshAI GACnn'nnGTC
Cuts at: 0 916 1206
Size: 916 290
PstI C_TGCA'G
Cuts at: 0 1034 1206
Size: 1034 172
PvuII CAG'CTG
Cuts at: 0 210 546 1087 1206
Size: 210 336 541 119
Fragments arranged by size:
541 336 210 119
SacI G_AGCT'C
Cuts at: 0 314 1206
Size: 314 892
SalI G'TCGA_C
Cuts at: 0 919 1206
Size: 919 287
SfcI C'TryA_G
Cuts at: 0 1030 1206
Size: 1030 176
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 100 1206
Size: 100 1106
StuI AGG'CCT
Cuts at: 0 145 1206
Size: 145 1061
StyI C'CwwG_G
Cuts at: 0 149 322 1206
Size: 149 173 884
Fragments arranged by size:
884 173 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 474 634 970 1206
Size: 474 160 336 236
Fragments arranged by size:
474 336 236 160
TatI w'GTAC_w
Cuts at: 0 302 1206
Size: 302 904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 903 1206
Size: 903 303
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaLI AvaI BanI
BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaWI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BsrGI BssSI BstAPI BstYI
Bsu36I BtgI EaeI EarI EcoICRI Eco57MI FalI HaeII
Hin4I HincII MscI MspA1I NcoI NspI PciI PflMI
PfoI PshAI PstI PvuII SacI SalI SfcI SfiI
StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BglII BlpI
BmtI BplI BpuEI BsaI BsaBI BsaHI BsaXI BsgI
BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BssHII BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI