(Linear) (Six-base) MAPSORT of: KIBB5687.seq  Check: 9484  from: 1  to: 732



KIBB5687.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     315     732 
   Size:        315     417
 
AclI AA'CG_TT

Cuts at:      0     611     732 
   Size:        611     121
 
ApoI r'AATT_y

Cuts at:      0     367     732 
   Size:        367     365
 
AvaI C'yCGr_G

Cuts at:      0     145     732 
   Size:        145     587
 
BamHI G'GATC_C

Cuts at:      0      48     732 
   Size:         48     684
 
BanII G_rGCy'C

Cuts at:      0     446     732 
   Size:        446     286
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     117     732 
   Size:        117     615
 
BglI GCCn_nnn'nGGC

Cuts at:      0     583     732 
   Size:        583     149
 
BlpI GC'TnA_GC

Cuts at:      0     531     732 
   Size:        531     201
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     316     732 
   Size:        316     416
 
BsaBI GATnn'nnATC

Cuts at:      0      53     732 
   Size:         53     679
 
BsaHI Gr'CG_yC

Cuts at:      0     463     732 
   Size:        463     269
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705     732 
   Size:        705      27
 
BsiHKAI G_wGCw'C

Cuts at:      0     446     732 
   Size:        446     286
 
BsmI GAATG_Cn'

Cuts at:      0     532     732 
   Size:        532     200
 
Bsp1286I G_dGCh'C

Cuts at:      0     446     732 
   Size:        446     286
 
BstYI r'GATC_y

Cuts at:      0      48     732 
   Size:         48     684
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     468     732 
   Size:        468     264
 
EaeI y'GGCC_r

Cuts at:      0     182     494     732 
   Size:        182     312     238

  Fragments arranged by size: 

                 312     238     182
 
EarI CTCTTCn'nnn_

Cuts at:      0       6     709     732 
   Size:          6     703      23

  Fragments arranged by size: 

                 703      23       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     250     732 
   Size:        250     482
 
EcoICRI GAG'CTC

Cuts at:      0     444     732 
   Size:        444     288
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     156     732 
   Size:        156     576
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     659     691     732 
   Size:        659      32      41

  Fragments arranged by size: 

                 659      41      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     524     732 
   Size:        524     208
 
MscI TGG'CCA

Cuts at:      0     496     732 
   Size:        496     236
 
MslI CAynn'nnrTG

Cuts at:      0     727     732 
   Size:        727       5
 
NspI r_CATG'y

Cuts at:      0     590     660     732 
   Size:        590      70      72

  Fragments arranged by size: 

                 590      72      70
 
PfoI T'CCnGG_A

Cuts at:      0     362     732 
   Size:        362     370
 
SacI G_AGCT'C

Cuts at:      0     446     732 
   Size:        446     286
 
SapI GCTCTTCn'nnn_

Cuts at:      0       6     709     732 
   Size:          6     703      23

  Fragments arranged by size: 

                 703      23       6
 
SfcI C'TryA_G

Cuts at:      0     161     732 
   Size:        161     571
 
StyI C'CwwG_G

Cuts at:      0     131     581     732 
   Size:        131     450     151

  Fragments arranged by size: 

                 450     151     131
 
TatI w'GTAC_w

Cuts at:      0     430     732 
   Size:        430     302
 
Tth111I GACn'n_nGTC

Cuts at:      0     256     334     732 
   Size:        256      78     398

  Fragments arranged by size: 

                 398     256      78
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     390     732 
   Size:        390     342
 

 Enzymes that do cut:

     AccI     AclI     ApoI     AvaI    BamHI    BanII     BbsI     BglI
     BlpI     BsaI    BsaBI    BsaHI     BsgI  BsiHKAI     BsmI Bsp1286I
    BstYI     DrdI     EaeI     EarI     EciI  EcoICRI     FalI    Hin4I
     MmeI     MscI     MslI     NspI     PfoI     SacI     SapI     SfcI
     StyI     TatI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     EagI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI     XbaI     XhoI     XmaI     XmnI     ZraI