Restriction map for
pF1KB5688
(Linear) (Six-base) MAPSORT of: KIBB5688.seq Check: 4590 from: 1 to: 2085
KIBB5688.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AatII G_ACGT'C
Cuts at: 0 1589 2085
Size: 1589 496
AccI GT'mk_AC
Cuts at: 0 575 2085
Size: 575 1510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 746 1510 1842 2085
Size: 247 499 764 332 243
Fragments arranged by size:
764 499 332 247 243
AleI CACnn'nnGTG
Cuts at: 0 304 2085
Size: 304 1781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 2085
Size: 61 2024
AlwNI CAG_nnn'CTG
Cuts at: 0 1119 1185 2085
Size: 1119 66 900
Fragments arranged by size:
1119 900 66
ApaI G_GGCC'C
Cuts at: 0 1843 2085
Size: 1843 242
ApoI r'AATT_y
Cuts at: 0 409 715 1499 2085
Size: 409 306 784 586
Fragments arranged by size:
784 586 409 306
AvaI C'yCGr_G
Cuts at: 0 1205 1816 2085
Size: 1205 611 269
Fragments arranged by size:
1205 611 269
BanI G'GyrC_C
Cuts at: 0 72 226 344 883 1031 1705 1960 2085
Size: 72 154 118 539 148 674 255 125
Fragments arranged by size:
674 539 255 154 148 125 118 72
BanII G_rGCy'C
Cuts at: 0 68 1523 1600 1843 2085
Size: 68 1455 77 243 242
Fragments arranged by size:
1455 243 242 77 68
BbeI G_GCGC'C
Cuts at: 0 76 2085
Size: 76 2009
BbsI GAAGACnn'nnnn_
Cuts at: 0 698 746 2085
Size: 698 48 1339
Fragments arranged by size:
1339 698 48
BbvCI CC'TCA_GC
Cuts at: 0 1942 2085
Size: 1942 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 91 125 955 989 2085
Size: 91 34 830 34 1096
Fragments arranged by size:
1096 830 91 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 293 2085
Size: 293 1792
BfrBI ATG'CAT
Cuts at: 0 183 2085
Size: 183 1902
BglII A'GATC_T
Cuts at: 0 732 2085
Size: 732 1353
BlpI GC'TnA_GC
Cuts at: 0 143 2085
Size: 143 1942
Bme1580I G_kGCm'C
Cuts at: 0 1843 2085
Size: 1843 242
BmrI ACTGGGnnnn_n'
Cuts at: 0 209 2085
Size: 209 1876
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1133 2085
Size: 1133 952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1267 1285 1694 2085
Size: 1042 225 18 409 391
Fragments arranged by size:
1042 409 391 225 18
Bpu10I CC'TnA_GC
Cuts at: 0 1010 1942 2085
Size: 1010 932 143
Fragments arranged by size:
1010 932 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 961 1186 1567 2085
Size: 961 225 381 518
Fragments arranged by size:
961 518 381 225
BsaAI yAC'GTr
Cuts at: 0 1434 2085
Size: 1434 651
BsaHI Gr'CG_yC
Cuts at: 0 73 1586 2085
Size: 73 1513 499
Fragments arranged by size:
1513 499 73
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 868 923 1618 1627 2085
Size: 868 55 695 9 458
Fragments arranged by size:
868 695 458 55 9
BseYI C'CCAG_C
Cuts at: 0 1647 2085
Size: 1647 438
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1559 2085
Size: 1559 526
BsiEI CG_ry'CG
Cuts at: 0 23 2085
Size: 23 2062
BsiHKAI G_wGCw'C
Cuts at: 0 1523 1600 1878 2085
Size: 1523 77 278 207
Fragments arranged by size:
1523 278 207 77
BsmI GAATG_Cn'
Cuts at: 0 1295 2085
Size: 1295 790
Bsp1286I G_dGCh'C
Cuts at: 0 68 1523 1600 1843 1878 2085
Size: 68 1455 77 243 35 207
Fragments arranged by size:
1455 243 207 77 68 35
BspHI T'CATG_A
Cuts at: 0 909 2085
Size: 909 1176
BsrBI CCG'CTC
Cuts at: 0 465 1827 2085
Size: 465 1362 258
Fragments arranged by size:
1362 465 258
BsrDI GCAATG_nn'
Cuts at: 0 164 370 1907 2085
Size: 164 206 1537 178
Fragments arranged by size:
1537 206 178 164
BsrFI r'CCGG_y
Cuts at: 0 91 2085
Size: 91 1994
BssSI C'ACGA_G
Cuts at: 0 400 2085
Size: 400 1685
BstAPI GCAn_nnn'nTGC
Cuts at: 0 118 2085
Size: 118 1967
BstXI CCAn_nnnn'nTGG
Cuts at: 0 586 2085
Size: 586 1499
BstYI r'GATC_y
Cuts at: 0 628 732 1616 1669 2085
Size: 628 104 884 53 416
Fragments arranged by size:
884 628 416 104 53
BtgI C'CryG_G
Cuts at: 0 17 32 585 1139 1282 1835 1913 1971
Size: 17 15 553 554 143 553 78 58
Cuts at: 1971 2037 2085
Size: 66 48
Fragments arranged by size:
554 553 553 143 78 66 58 48
17 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1276 1881 2085
Size: 1276 605 204
Fragments arranged by size:
1276 605 204
BtsI GCAGTG_nn'
Cuts at: 0 1556 1843 1878 1907 2085
Size: 1556 287 35 29 178
Fragments arranged by size:
1556 287 178 35 29
EaeI y'GGCC_r
Cuts at: 0 5 20 89 1904 2085
Size: 5 15 69 1815 181
Fragments arranged by size:
1815 181 69 15 5
EagI C'GGCC_G
Cuts at: 0 20 2085
Size: 20 2065
EarI CTCTTCn'nnn_
Cuts at: 0 638 644 656 662 668 680 1064 1706
Size: 638 6 12 6 6 12 384 642
Cuts at: 1706 1817 1942 2003 2085
Size: 111 125 61 82
Fragments arranged by size:
642 638 384 125 111 82 61 12
12 6 6 6
EcoICRI GAG'CTC
Cuts at: 0 1521 1598 2085
Size: 1521 77 487
Fragments arranged by size:
1521 487 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 746 1042 1267 1285 1510 1694 1842
Size: 247 499 296 225 18 225 184 148
Cuts at: 1842 2085
Size: 243
Fragments arranged by size:
499 296 247 243 225 225 184 148
18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 255 277 393 2085
Size: 255 22 116 1692
Fragments arranged by size:
1692 255 116 22
HaeII r_GCGC'y
Cuts at: 0 76 88 2085
Size: 76 12 1997
Fragments arranged by size:
1997 76 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1410 1442 1816 1848 2085
Size: 1410 32 374 32 237
Fragments arranged by size:
1410 374 237 32 32
HincII GTy'rAC
Cuts at: 0 1383 2085
Size: 1383 702
HindIII A'AGCT_T
Cuts at: 0 1148 2085
Size: 1148 937
KasI G'GCGC_C
Cuts at: 0 72 2085
Size: 72 2013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 952 1216 1788 2085
Size: 244 708 264 572 297
Fragments arranged by size:
708 572 297 264 244
MscI TGG'CCA
Cuts at: 0 1906 2085
Size: 1906 179
MslI CAynn'nnrTG
Cuts at: 0 304 1900 2042 2085
Size: 304 1596 142 43
Fragments arranged by size:
1596 304 142 43
MspA1I CmG'CkG
Cuts at: 0 19 324 757 1020 1185 2085
Size: 19 305 433 263 165 900
Fragments arranged by size:
900 433 305 263 165 19
NaeI GCC'GGC
Cuts at: 0 93 2085
Size: 93 1992
NarI GG'CG_CC
Cuts at: 0 73 2085
Size: 73 2012
NcoI C'CATG_G
Cuts at: 0 585 1139 2037 2085
Size: 585 554 898 48
Fragments arranged by size:
898 585 554 48
NgoMIV G'CCGG_C
Cuts at: 0 91 2085
Size: 91 1994
NotI GC'GGCC_GC
Cuts at: 0 20 2085
Size: 20 2065
NsiI A_TGCA'T
Cuts at: 0 185 2085
Size: 185 1900
NspI r_CATG'y
Cuts at: 0 187 521 558 994 1372 2085
Size: 187 334 37 436 378 713
Fragments arranged by size:
713 436 378 334 187 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 1140 2085
Size: 1140 945
PmlI CAC'GTG
Cuts at: 0 1434 2085
Size: 1434 651
PshAI GACnn'nnGTC
Cuts at: 0 1232 1859 2085
Size: 1232 627 226
Fragments arranged by size:
1232 627 226
PsiI TTA'TAA
Cuts at: 0 707 2085
Size: 707 1378
PspOMI G'GGCC_C
Cuts at: 0 1839 2085
Size: 1839 246
PspXI vC'TCGA_Gb
Cuts at: 0 1816 2085
Size: 1816 269
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2041 2073 2085
Size: 2041 32 12
Fragments arranged by size:
2041 32 12
PstI C_TGCA'G
Cuts at: 0 978 2085
Size: 978 1107
PvuII CAG'CTG
Cuts at: 0 757 1020 1185 2085
Size: 757 263 165 900
Fragments arranged by size:
900 757 263 165
SacI G_AGCT'C
Cuts at: 0 1523 1600 2085
Size: 1523 77 485
Fragments arranged by size:
1523 485 77
SacII CC_GC'GG
Cuts at: 0 20 2085
Size: 20 2065
SapI GCTCTTCn'nnn_
Cuts at: 0 1817 2003 2085
Size: 1817 186 82
Fragments arranged by size:
1817 186 82
ScaI AGT'ACT
Cuts at: 0 268 2085
Size: 268 1817
SfcI C'TryA_G
Cuts at: 0 292 974 2085
Size: 292 682 1111
Fragments arranged by size:
1111 682 292
SfoI GGC'GCC
Cuts at: 0 74 2085
Size: 74 2011
SmlI C'TyrA_G
Cuts at: 0 1816 2085
Size: 1816 269
SphI G_CATG'C
Cuts at: 0 558 994 2085
Size: 558 436 1091
Fragments arranged by size:
1091 558 436
StuI AGG'CCT
Cuts at: 0 1306 1814 2085
Size: 1306 508 271
Fragments arranged by size:
1306 508 271
StyI C'CwwG_G
Cuts at: 0 80 585 1139 2037 2085
Size: 80 505 554 898 48
Fragments arranged by size:
898 554 505 80 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 394 824 2085
Size: 394 430 1261
Fragments arranged by size:
1261 430 394
TatI w'GTAC_w
Cuts at: 0 266 2085
Size: 266 1819
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1661 1693 2085
Size: 1661 32 392
Fragments arranged by size:
1661 392 32
XhoI C'TCGA_G
Cuts at: 0 1816 2085
Size: 1816 269
XmnI GAAnn'nnTTC
Cuts at: 0 257 260 2085
Size: 257 3 1825
Fragments arranged by size:
1825 257 3
ZraI GAC'GTC
Cuts at: 0 1587 2085
Size: 1587 498
Enzymes that do cut:
AatII AccI AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
EcoICRI Eco57MI FalI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NsiI NspI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI PstI PvuII SacI SacII SapI ScaI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BclI BglI BmgBI BmtI BpuEI BsaBI BsaWI BsaXI
BsiWI BsmBI BspEI BspMI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HpaI
KpnI MfeI MluI NdeI NheI NruI PacI PasI
PciI PfoI PmeI PpiI PpuMI PvuI RsrII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XcmI XmaI