(Linear) (Six-base) MAPSORT of: KIBB5696.seq  Check: 2920  from: 1  to: 735



KIBB5696.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     731     735 
   Size:        731       4
 
AvaI C'yCGr_G

Cuts at:      0     236     735 
   Size:        236     499
 
BanII G_rGCy'C

Cuts at:      0     162     735 
   Size:        162     573
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339     735 
   Size:        339     396
 
BbvCI CC'TCA_GC

Cuts at:      0     389     735 
   Size:        389     346
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     217     251     735 
   Size:        217      34     484

  Fragments arranged by size: 

                 484     217      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     684     735 
   Size:        684      51
 
BglI GCCn_nnn'nGGC

Cuts at:      0      34      43     735 
   Size:         34       9     692

  Fragments arranged by size: 

                 692      34       9
 
BlpI GC'TnA_GC

Cuts at:      0      47     735 
   Size:         47     688
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     511     735 
   Size:        511     224
 
Bpu10I CC'TnA_GC

Cuts at:      0     389     735 
   Size:        389     346
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     735 
   Size:        171     564
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     735 
   Size:        130     605
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     189     253     695     735 
   Size:        189      64     442      40

  Fragments arranged by size: 

                 442     189      64      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     162     735 
   Size:        162     573
 
BsrDI GCAATG_nn'

Cuts at:      0     288     596     735 
   Size:        288     308     139

  Fragments arranged by size: 

                 308     288     139
 
EaeI y'GGCC_r

Cuts at:      0      35     735 
   Size:         35     700
 
EarI CTCTTCn'nnn_

Cuts at:      0     617     735 
   Size:        617     118
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     731     735 
   Size:        731       4
 
EcoRV GAT'ATC

Cuts at:      0     276     735 
   Size:        276     459
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     277     309     411     443     735 
   Size:        277      32     102      32     292

  Fragments arranged by size: 

                 292     277     102      32      32
 
HindIII A'AGCT_T

Cuts at:      0     452     735 
   Size:        452     283
 
MspA1I CmG'CkG

Cuts at:      0     400     735 
   Size:        400     335
 
PsiI TTA'TAA

Cuts at:      0     143     735 
   Size:        143     592
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      34     735 
   Size:         34     701
 
SmlI C'TyrA_G

Cuts at:      0     186     236     735 
   Size:        186      50     499

  Fragments arranged by size: 

                 499     186      50
 
StyI C'CwwG_G

Cuts at:      0     696     735 
   Size:        696      39
 
TatI w'GTAC_w

Cuts at:      0     288     735 
   Size:        288     447
 
Tth111I GACn'n_nGTC

Cuts at:      0     503     735 
   Size:        503     232
 
XhoI C'TCGA_G

Cuts at:      0     236     735 
   Size:        236     499
 

 Enzymes that do cut:

     AcuI     AvaI    BanII     BbsI    BbvCI     BcgI    BciVI     BglI
     BlpI     BmrI   Bpu10I    BpuEI    BseRI    BsmBI Bsp1286I    BsrDI
     EaeI     EarI  Eco57MI    EcoRV    Hin4I  HindIII   MspA1I     PsiI
     SfiI     SmlI     StyI     TatI  Tth111I     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BclI    BfrBI    BglII Bme1580I    BmgBI     BmtI     BplI
     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI     XbaI     XcmI     XmaI     XmnI     ZraI