Restriction map for
pF1KB5705
(Linear) (Six-base) MAPSORT of: KIBB5705.seq Check: 3393 from: 1 to: 2232
KIBB5705.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AatII G_ACGT'C
Cuts at: 0 196 2232
Size: 196 2036
AccI GT'mk_AC
Cuts at: 0 1748 1775 2232
Size: 1748 27 457
Fragments arranged by size:
1748 457 27
Acc65I G'GTAC_C
Cuts at: 0 83 2232
Size: 83 2149
AfeI AGC'GCT
Cuts at: 0 1077 2232
Size: 1077 1155
AhdI GACnn_n'nnGTC
Cuts at: 0 1782 2232
Size: 1782 450
AleI CACnn'nnGTG
Cuts at: 0 1612 2232
Size: 1612 620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 463 835 1348 2024 2232
Size: 463 372 513 676 208
Fragments arranged by size:
676 513 463 372 208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 2232
Size: 37 32 2163
Fragments arranged by size:
2163 37 32
AlwNI CAG_nnn'CTG
Cuts at: 0 57 71 162 567 654 752 774 2154
Size: 57 14 91 405 87 98 22 1380
Cuts at: 2154 2232
Size: 78
Fragments arranged by size:
1380 405 98 91 87 78 57 22
14
ApaI G_GGCC'C
Cuts at: 0 288 1732 1874 2232
Size: 288 1444 142 358
Fragments arranged by size:
1444 358 288 142
ApoI r'AATT_y
Cuts at: 0 1453 2232
Size: 1453 779
AvaI C'yCGr_G
Cuts at: 0 496 2232
Size: 496 1736
BanI G'GyrC_C
Cuts at: 0 83 273 1125 2232
Size: 83 190 852 1107
Fragments arranged by size:
1107 852 190 83
BanII G_rGCy'C
Cuts at: 0 288 787 1415 1732 1874 2232
Size: 288 499 628 317 142 358
Fragments arranged by size:
628 499 358 317 288 142
BbsI GAAGACnn'nnnn_
Cuts at: 0 1410 2232
Size: 1410 822
BbvCI CC'TCA_GC
Cuts at: 0 451 2232
Size: 451 1781
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2147 2181 2232
Size: 2147 34 51
Fragments arranged by size:
2147 51 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1109 1727 2232
Size: 1109 618 505
Fragments arranged by size:
1109 618 505
BlpI GC'TnA_GC
Cuts at: 0 1079 2232
Size: 1079 1153
Bme1580I G_kGCm'C
Cuts at: 0 94 288 1254 1732 1874 2232
Size: 94 194 966 478 142 358
Fragments arranged by size:
966 478 358 194 142 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 2070 2232
Size: 2070 162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1981 2232
Size: 1981 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 688 2212 2232
Size: 643 45 1524 20
Fragments arranged by size:
1524 643 45 20
Bpu10I CC'TnA_GC
Cuts at: 0 451 2232
Size: 451 1781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 2232
Size: 919 1313
BsaI GGTCTCn'nnnn_
Cuts at: 0 664 2232
Size: 664 1568
BsaHI Gr'CG_yC
Cuts at: 0 193 215 2232
Size: 193 22 2017
Fragments arranged by size:
2017 193 22
BsaWI w'CCGG_w
Cuts at: 0 1122 1751 2232
Size: 1122 629 481
Fragments arranged by size:
1122 629 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1730 1760 2012 2042 2232
Size: 1730 30 252 30 190
Fragments arranged by size:
1730 252 190 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1186 2232
Size: 1186 1046
BseYI C'CCAG_C
Cuts at: 0 1357 2232
Size: 1357 875
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 626 947 1109 2232
Size: 97 529 321 162 1123
Fragments arranged by size:
1123 529 321 162 97
BsiEI CG_ry'CG
Cuts at: 0 922 1494 2232
Size: 922 572 738
Fragments arranged by size:
922 738 572
BsiHKAI G_wGCw'C
Cuts at: 0 929 1415 2232
Size: 929 486 817
Fragments arranged by size:
929 817 486
BsmI GAATG_Cn'
Cuts at: 0 882 2232
Size: 882 1350
BsmBI CGTCTCn'nnnn_
Cuts at: 0 222 385 2232
Size: 222 163 1847
Fragments arranged by size:
1847 222 163
Bsp1286I G_dGCh'C
Cuts at: 0 94 288 787 929 1254 1415 1732 1874
Size: 94 194 499 142 325 161 317 142
Cuts at: 1874 2232
Size: 358
Fragments arranged by size:
499 358 325 317 194 161 142 142
94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1277 2232
Size: 1277 955
BsrBI CCG'CTC
Cuts at: 0 828 864 2232
Size: 828 36 1368
Fragments arranged by size:
1368 828 36
BsrDI GCAATG_nn'
Cuts at: 0 269 2001 2232
Size: 269 1732 231
Fragments arranged by size:
1732 269 231
BsrFI r'CCGG_y
Cuts at: 0 185 1099 2232
Size: 185 914 1133
Fragments arranged by size:
1133 914 185
BsrGI T'GTAC_A
Cuts at: 0 1166 1367 1943 2232
Size: 1166 201 576 289
Fragments arranged by size:
1166 576 289 201
BssHII G'CGCG_C
Cuts at: 0 1174 2232
Size: 1174 1058
BssSI C'ACGA_G
Cuts at: 0 951 2232
Size: 951 1281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 71 538 633 752 774 2204 2232
Size: 71 467 95 119 22 1430 28
Fragments arranged by size:
1430 467 119 95 71 28 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1147 1614 1740 1813 2232
Size: 1147 467 126 73 419
Fragments arranged by size:
1147 467 419 126 73
Bsu36I CC'TnA_GG
Cuts at: 0 1685 2232
Size: 1685 547
BtgI C'CryG_G
Cuts at: 0 980 1842 1983 2232
Size: 980 862 141 249
Fragments arranged by size:
980 862 249 141
BtsI GCAGTG_nn'
Cuts at: 0 223 631 718 772 940 959 1352 1625
Size: 223 408 87 54 168 19 393 273
Cuts at: 1625 2202 2232
Size: 577 30
Fragments arranged by size:
577 408 393 273 223 168 87 54
30 19
DraI TTT'AAA
Cuts at: 0 2211 2232
Size: 2211 21
EaeI y'GGCC_r
Cuts at: 0 327 395 510 865 977 1491 1716 1742
Size: 327 68 115 355 112 514 225 26
Cuts at: 1742 1827 2172 2232
Size: 85 345 60
Fragments arranged by size:
514 355 345 327 225 115 112 85
68 60 26
EagI C'GGCC_G
Cuts at: 0 1491 2232
Size: 1491 741
EarI CTCTTCn'nnn_
Cuts at: 0 1972 2232
Size: 1972 260
EcoICRI GAG'CTC
Cuts at: 0 1413 2232
Size: 1413 819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 688 2212 2232
Size: 643 45 1524 20
Fragments arranged by size:
1524 643 45 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 997 2232
Size: 256 741 1235
Fragments arranged by size:
1235 741 256
EcoO109I rG'GnC_Cy
Cuts at: 0 284 300 588 618 990 1266 1729 1870
Size: 284 16 288 30 372 276 463 141
Cuts at: 1870 1871 2151 2232
Size: 1 280 81
Fragments arranged by size:
463 372 288 284 280 276 141 81
30 16 1
EcoRI G'AATT_C
Cuts at: 0 1453 2232
Size: 1453 779
FspI TGC'GCA
Cuts at: 0 811 1066 2232
Size: 811 255 1166
Fragments arranged by size:
1166 811 255
FspAI rTGC'GCAy
Cuts at: 0 1066 2232
Size: 1066 1166
HaeII r_GCGC'y
Cuts at: 0 492 959 1006 1079 1299 1605 2232
Size: 492 467 47 73 220 306 627
Fragments arranged by size:
627 492 467 306 220 73 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 911 943 1768 1800 2232
Size: 911 32 825 32 432
Fragments arranged by size:
911 825 432 32 32
HincII GTy'rAC
Cuts at: 0 1776 2232
Size: 1776 456
KpnI G_GTAC'C
Cuts at: 0 87 2232
Size: 87 2145
MfeI C'AATT_G
Cuts at: 0 536 1400 2232
Size: 536 864 832
Fragments arranged by size:
864 832 536
MscI TGG'CCA
Cuts at: 0 979 2174 2232
Size: 979 1195 58
Fragments arranged by size:
1195 979 58
MslI CAynn'nnrTG
Cuts at: 0 875 1145 1612 1642 2050 2232
Size: 875 270 467 30 408 182
Fragments arranged by size:
875 467 408 270 182 30
MspA1I CmG'CkG
Cuts at: 0 525 567 711 885 1490 1596 2232
Size: 525 42 144 174 605 106 636
Fragments arranged by size:
636 605 525 174 144 106 42
NaeI GCC'GGC
Cuts at: 0 187 2232
Size: 187 2045
NcoI C'CATG_G
Cuts at: 0 1983 2232
Size: 1983 249
NdeI CA'TA_TG
Cuts at: 0 1155 2232
Size: 1155 1077
NgoMIV G'CCGG_C
Cuts at: 0 185 2232
Size: 185 2047
NotI GC'GGCC_GC
Cuts at: 0 1491 2232
Size: 1491 741
NspI r_CATG'y
Cuts at: 0 822 2232
Size: 822 1410
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1881 2140 2181 2232
Size: 42 1839 259 41 51
Fragments arranged by size:
1839 259 51 42 41
PfoI T'CCnGG_A
Cuts at: 0 857 2232
Size: 857 1375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 1678 1710 2232
Size: 1537 32 109 32 522
Fragments arranged by size:
1537 522 109 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 300 618 1266 2232
Size: 300 318 648 966
Fragments arranged by size:
966 648 318 300
PspOMI G'GGCC_C
Cuts at: 0 284 1728 1870 2232
Size: 284 1444 142 362
Fragments arranged by size:
1444 362 284 142
PspXI vC'TCGA_Gb
Cuts at: 0 496 2232
Size: 496 1736
PstI C_TGCA'G
Cuts at: 0 230 488 572 638 757 1601 2130 2232
Size: 230 258 84 66 119 844 529 102
Fragments arranged by size:
844 529 258 230 119 102 84 66
PvuI CG_AT'CG
Cuts at: 0 922 2232
Size: 922 1310
PvuII CAG'CTG
Cuts at: 0 525 567 711 885 1596 2232
Size: 525 42 144 174 711 636
Fragments arranged by size:
711 636 525 174 144 42
SacI G_AGCT'C
Cuts at: 0 1415 2232
Size: 1415 817
SalI G'TCGA_C
Cuts at: 0 1774 2232
Size: 1774 458
SfcI C'TryA_G
Cuts at: 0 226 484 568 634 753 1597 2126 2232
Size: 226 258 84 66 119 844 529 106
Fragments arranged by size:
844 529 258 226 119 106 84 66
SmlI C'TyrA_G
Cuts at: 0 496 898 2232
Size: 496 402 1334
Fragments arranged by size:
1334 496 402
StuI AGG'CCT
Cuts at: 0 385 853 2232
Size: 385 468 1379
Fragments arranged by size:
1379 468 385
StyI C'CwwG_G
Cuts at: 0 95 1733 1983 2232
Size: 95 1638 250 249
Fragments arranged by size:
1638 250 249 95
TatI w'GTAC_w
Cuts at: 0 1166 1367 1943 2232
Size: 1166 201 576 289
Fragments arranged by size:
1166 576 289 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 1104 1136 1208 1240 2012 2044
Size: 839 32 233 32 72 32 772 32
Cuts at: 2044 2232
Size: 188
Fragments arranged by size:
839 772 233 188 72 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1818 2232
Size: 1818 414
XhoI C'TCGA_G
Cuts at: 0 496 2232
Size: 496 1736
XmnI GAAnn'nnTTC
Cuts at: 0 235 2232
Size: 235 1997
ZraI GAC'GTC
Cuts at: 0 194 2232
Size: 194 2038
Enzymes that do cut:
AatII AccI Acc65I AfeI AhdI AleI AlfI AloI
AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI
BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstXI Bsu36I BtgI
BtsI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII KpnI
MfeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NotI NspI PflMI PfoI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII SacI SalI SfcI SmlI StuI
StyI TatI TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AclI AcuI AflII AflIII AgeI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BglII BmgBI BmtI BsaAI BsaBI BsiWI BspEI
BspHI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraIII DrdI EciI EcoRV FalI FseI HindIII HpaI
KasI MluI MmeI NarI NheI NruI NsiI PacI
PasI PciI PmeI PmlI PshAI PsiI PsrI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XmaI