Restriction map for
pF1KB5709
(Linear) (Six-base) MAPSORT of: KIBB5709.seq Check: 288 from: 1 to: 2352
KIBB5709.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1635 1943 2052 2335 2352
Size: 79 1556 308 109 283 17
Fragments arranged by size:
1556 308 283 109 79 17
AfeI AGC'GCT
Cuts at: 0 1828 2352
Size: 1828 524
AflIII A'CryG_T
Cuts at: 0 266 2352
Size: 266 2086
AleI CACnn'nnGTG
Cuts at: 0 1792 2352
Size: 1792 560
AlwNI CAG_nnn'CTG
Cuts at: 0 2188 2352
Size: 2188 164
ApaI G_GGCC'C
Cuts at: 0 1300 1808 1868 2352
Size: 1300 508 60 484
Fragments arranged by size:
1300 508 484 60
AvaI C'yCGr_G
Cuts at: 0 1259 2352
Size: 1259 1093
BamHI G'GATC_C
Cuts at: 0 743 2352
Size: 743 1609
BanI G'GyrC_C
Cuts at: 0 453 2352
Size: 453 1899
BanII G_rGCy'C
Cuts at: 0 578 1300 1682 1808 1868 2198 2213 2352
Size: 578 722 382 126 60 330 15 139
Fragments arranged by size:
722 578 382 330 139 126 60 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 1898 2084 2352
Size: 125 1773 186 268
Fragments arranged by size:
1773 268 186 125
BbvCI CC'TCA_GC
Cuts at: 0 1319 1535 2189 2352
Size: 1319 216 654 163
Fragments arranged by size:
1319 654 216 163
BclI T'GATC_A
Cuts at: 0 1070 2352
Size: 1070 1282
BglII A'GATC_T
Cuts at: 0 1004 2352
Size: 1004 1348
Bme1580I G_kGCm'C
Cuts at: 0 1300 1808 1868 2347 2352
Size: 1300 508 60 479 5
Fragments arranged by size:
1300 508 479 60 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 1729 2352
Size: 1729 623
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1884 2352
Size: 1884 468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1234 1363 1696 1884 2352
Size: 863 371 129 333 188 468
Fragments arranged by size:
863 468 371 333 188 129
Bpu10I CC'TnA_GC
Cuts at: 0 628 1319 1535 2189 2352
Size: 628 691 216 654 163
Fragments arranged by size:
691 654 628 216 163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1252 1329 2088 2352
Size: 763 489 77 759 264
Fragments arranged by size:
763 759 489 264 77
BsaWI w'CCGG_w
Cuts at: 0 515 740 2352
Size: 515 225 1612
Fragments arranged by size:
1612 515 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1914 1944 2352
Size: 1914 30 408
Fragments arranged by size:
1914 408 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1366 1552 1555 2020 2023 2203 2206 2352
Size: 1366 186 3 465 3 180 3 146
Fragments arranged by size:
1366 465 186 180 146 3 3 3
BseYI C'CCAG_C
Cuts at: 0 646 913 1220 1681 1809 2052 2090 2352
Size: 646 267 307 461 128 243 38 262
Fragments arranged by size:
646 461 307 267 262 243 128 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1117 1609 2352
Size: 985 132 492 743
Fragments arranged by size:
985 743 492 132
BsiEI CG_ry'CG
Cuts at: 0 2271 2352
Size: 2271 81
BsiHKAI G_wGCw'C
Cuts at: 0 578 634 812 2213 2352
Size: 578 56 178 1401 139
Fragments arranged by size:
1401 578 178 139 56
Bsp1286I G_dGCh'C
Cuts at: 0 578 634 812 1300 1682 1808 1868 2198
Size: 578 56 178 488 382 126 60 330
Cuts at: 2198 2213 2347 2352
Size: 15 134 5
Fragments arranged by size:
578 488 382 330 178 134 126 60
56 15 5
BspEI T'CCGG_A
Cuts at: 0 515 2352
Size: 515 1837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1472 1556 2352
Size: 1472 84 796
Fragments arranged by size:
1472 796 84
BsrBI CCG'CTC
Cuts at: 0 1264 1374 2352
Size: 1264 110 978
Fragments arranged by size:
1264 978 110
BsrGI T'GTAC_A
Cuts at: 0 754 2352
Size: 754 1598
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1572 2352
Size: 1572 780
BstEII G'GTnAC_C
Cuts at: 0 2122 2352
Size: 2122 230
BstXI CCAn_nnnn'nTGG
Cuts at: 0 286 1908 2352
Size: 286 1622 444
Fragments arranged by size:
1622 444 286
BstYI r'GATC_y
Cuts at: 0 63 743 1004 1385 1719 2352
Size: 63 680 261 381 334 633
Fragments arranged by size:
680 633 381 334 261 63
BtgI C'CryG_G
Cuts at: 0 669 1193 1424 1907 2352
Size: 669 524 231 483 445
Fragments arranged by size:
669 524 483 445 231
BtsI GCAGTG_nn'
Cuts at: 0 392 531 711 759 1123 2024 2269 2352
Size: 392 139 180 48 364 901 245 83
Fragments arranged by size:
901 392 364 245 180 139 83 48
ClaI AT'CG_AT
Cuts at: 0 2228 2352
Size: 2228 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 475 2352
Size: 127 348 1877
Fragments arranged by size:
1877 348 127
EaeI y'GGCC_r
Cuts at: 0 1265 1818 2268 2327 2352
Size: 1265 553 450 59 25
Fragments arranged by size:
1265 553 450 59 25
EagI C'GGCC_G
Cuts at: 0 2268 2352
Size: 2268 84
EarI CTCTTCn'nnn_
Cuts at: 0 1180 1734 1907 1991 2201 2352
Size: 1180 554 173 84 210 151
Fragments arranged by size:
1180 554 210 173 151 84
EcoICRI GAG'CTC
Cuts at: 0 576 2211 2352
Size: 576 1635 141
Fragments arranged by size:
1635 576 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 863 1234 1363 1635 1696 1884 1943
Size: 79 784 371 129 272 61 188 59
Cuts at: 1943 2052 2335 2352
Size: 109 283 17
Fragments arranged by size:
784 371 283 272 188 129 109 79
61 59 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1937 2278 2352
Size: 1937 341 74
Fragments arranged by size:
1937 341 74
EcoO109I rG'GnC_Cy
Cuts at: 0 221 1029 1296 1297 1545 1805 1864 1865
Size: 221 808 267 1 248 260 59 1
Cuts at: 1865 2352
Size: 487
Fragments arranged by size:
808 487 267 260 248 221 59 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1023 2352
Size: 1023 1329
FspI TGC'GCA
Cuts at: 0 1447 2352
Size: 1447 905
FspAI rTGC'GCAy
Cuts at: 0 1447 2352
Size: 1447 905
HaeII r_GCGC'y
Cuts at: 0 1830 2352
Size: 1830 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 288 320 674 706 1267 1299
Size: 55 32 201 32 354 32 561 32
Cuts at: 1299 1711 1743 2286 2318 2352
Size: 412 32 543 32 34
Fragments arranged by size:
561 543 412 354 201 55 34 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 465 867 2352
Size: 465 402 1485
Fragments arranged by size:
1485 465 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1568 2352
Size: 335 1233 784
Fragments arranged by size:
1233 784 335
MscI TGG'CCA
Cuts at: 0 1820 2329 2352
Size: 1820 509 23
Fragments arranged by size:
1820 509 23
MslI CAynn'nnrTG
Cuts at: 0 892 944 1792 2352
Size: 892 52 848 560
Fragments arranged by size:
892 848 560 52
MspA1I CmG'CkG
Cuts at: 0 749 1426 1473 1554 1563 1674 2352
Size: 749 677 47 81 9 111 678
Fragments arranged by size:
749 678 677 111 81 47 9
NcoI C'CATG_G
Cuts at: 0 669 1907 2352
Size: 669 1238 445
Fragments arranged by size:
1238 669 445
NotI GC'GGCC_GC
Cuts at: 0 2268 2352
Size: 2268 84
NspI r_CATG'y
Cuts at: 0 270 943 1848 2352
Size: 270 673 905 504
Fragments arranged by size:
905 673 504 270
PasI CC'CwG_GG
Cuts at: 0 19 2352
Size: 19 2333
PciI A'CATG_T
Cuts at: 0 266 2352
Size: 266 2086
PflMI CCAn_nnn'nTGG
Cuts at: 0 496 1796 1816 2352
Size: 496 1300 20 536
Fragments arranged by size:
1300 536 496 20
PfoI T'CCnGG_A
Cuts at: 0 719 1522 2352
Size: 719 803 830
Fragments arranged by size:
830 803 719
PpuMI rG'GwC_Cy
Cuts at: 0 221 1029 1545 2352
Size: 221 808 516 807
Fragments arranged by size:
808 807 516 221
PspOMI G'GGCC_C
Cuts at: 0 1296 1804 1864 2352
Size: 1296 508 60 488
Fragments arranged by size:
1296 508 488 60
PstI C_TGCA'G
Cuts at: 0 71 1064 1457 1478 1553 1568 1941 2222
Size: 71 993 393 21 75 15 373 281
Cuts at: 2222 2352
Size: 130
Fragments arranged by size:
993 393 373 281 130 75 71 21
15
PvuII CAG'CTG
Cuts at: 0 1473 1563 1674 2352
Size: 1473 90 111 678
Fragments arranged by size:
1473 678 111 90
SacI G_AGCT'C
Cuts at: 0 578 2213 2352
Size: 578 1635 139
Fragments arranged by size:
1635 578 139
SacII CC_GC'GG
Cuts at: 0 1427 2352
Size: 1427 925
SanDI GG'GwC_CC
Cuts at: 0 221 2352
Size: 221 2131
SapI GCTCTTCn'nnn_
Cuts at: 0 1734 2201 2352
Size: 1734 467 151
Fragments arranged by size:
1734 467 151
SfcI C'TryA_G
Cuts at: 0 67 927 1060 1453 1474 1549 1564 1937
Size: 67 860 133 393 21 75 15 373
Cuts at: 1937 2218 2352
Size: 281 134
Fragments arranged by size:
860 393 373 281 134 133 75 67
21 15
SmlI C'TyrA_G
Cuts at: 0 778 1231 1259 1308 2103 2352
Size: 778 453 28 49 795 249
Fragments arranged by size:
795 778 453 249 49 28
SphI G_CATG'C
Cuts at: 0 1848 2352
Size: 1848 504
StyI C'CwwG_G
Cuts at: 0 225 457 590 669 1013 1418 1907 2352
Size: 225 232 133 79 344 405 489 445
Fragments arranged by size:
489 445 405 344 232 225 133 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 649 672 705 1628 1714 2352
Size: 649 23 33 923 86 638
Fragments arranged by size:
923 649 638 86 33 23
TatI w'GTAC_w
Cuts at: 0 754 1979 2352
Size: 754 1225 373
Fragments arranged by size:
1225 754 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1911 1943 2352
Size: 1911 32 409
Fragments arranged by size:
1911 409 32
Tth111I GACn'n_nGTC
Cuts at: 0 1638 2352
Size: 1638 714
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 2352
Size: 1275 1077
XhoI C'TCGA_G
Cuts at: 0 1259 2352
Size: 1259 1093
XmnI GAAnn'nnTTC
Cuts at: 0 505 2032 2352
Size: 505 1527 320
Fragments arranged by size:
1527 505 320
Enzymes that do cut:
AcuI AfeI AflIII AleI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BbvCI BclI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrGI
BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NcoI NotI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SfcI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BglI BlpI BmgBI
BmtI BsaI BsaAI BsaBI BsaHI BsiWI BsmI BsmBI
BspHI BsrDI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I
BtgZI CspCI DraI DraIII EciI EcoRI EcoRV FseI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XmaI
ZraI