Restriction map for
pF1KB5713
(Linear) (Six-base) MAPSORT of: KIBB5713.seq Check: 6605 from: 1 to: 2355
KIBB5713.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 0 794 1860 2355
Size: 794 1066 495
Fragments arranged by size:
1066 794 495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 494 1460 2271 2355
Size: 449 45 966 811 84
Fragments arranged by size:
966 811 449 84 45
AflIII A'CryG_T
Cuts at: 0 147 2355
Size: 147 2208
AleI CACnn'nnGTG
Cuts at: 0 2224 2355
Size: 2224 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 876 2355
Size: 876 1479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2027 2059 2355
Size: 2027 32 296
Fragments arranged by size:
2027 296 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 2012 2355
Size: 300 1712 343
Fragments arranged by size:
1712 343 300
ApaLI G'TGCA_C
Cuts at: 0 2227 2355
Size: 2227 128
ApoI r'AATT_y
Cuts at: 0 189 611 685 989 1054 2098 2326 2355
Size: 189 422 74 304 65 1044 228 29
Fragments arranged by size:
1044 422 304 228 189 74 65 29
BanII G_rGCy'C
Cuts at: 0 37 123 1977 2355
Size: 37 86 1854 378
Fragments arranged by size:
1854 378 86 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 482 1202 1327 1448 1730 1929 1945
Size: 437 45 720 125 121 282 199 16
Cuts at: 1945 2355
Size: 410
Fragments arranged by size:
720 437 410 282 199 125 121 45
16
BbvCI CC'TCA_GC
Cuts at: 0 110 1349 2355
Size: 110 1239 1006
Fragments arranged by size:
1239 1006 110
BglII A'GATC_T
Cuts at: 0 407 2355
Size: 407 1948
Bme1580I G_kGCm'C
Cuts at: 0 2231 2355
Size: 2231 124
BmrI ACTGGGnnnn_n'
Cuts at: 0 1641 1811 2355
Size: 1641 170 544
Fragments arranged by size:
1641 544 170
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 2355
Size: 210 2145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2113 2355
Size: 2113 242
Bpu10I CC'TnA_GC
Cuts at: 0 110 1349 2355
Size: 110 1239 1006
Fragments arranged by size:
1239 1006 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1880 2355
Size: 1165 715 475
Fragments arranged by size:
1165 715 475
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 1393 2355
Size: 266 1127 962
Fragments arranged by size:
1127 962 266
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1514 1544 2027 2057 2355
Size: 1514 30 483 30 298
Fragments arranged by size:
1514 483 298 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1517 1729 2355
Size: 1517 212 626
Fragments arranged by size:
1517 626 212
BseYI C'CCAG_C
Cuts at: 0 57 1296 2355
Size: 57 1239 1059
Fragments arranged by size:
1239 1059 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 1358 2355
Size: 1067 291 997
Fragments arranged by size:
1067 997 291
BsiHKAI G_wGCw'C
Cuts at: 0 1977 2231 2355
Size: 1977 254 124
Fragments arranged by size:
1977 254 124
BsmI GAATG_Cn'
Cuts at: 0 865 2355
Size: 865 1490
Bsp1286I G_dGCh'C
Cuts at: 0 37 123 1977 2231 2355
Size: 37 86 1854 254 124
Fragments arranged by size:
1854 254 124 86 37
BspHI T'CATG_A
Cuts at: 0 1308 1511 2355
Size: 1308 203 844
Fragments arranged by size:
1308 844 203
BsrGI T'GTAC_A
Cuts at: 0 2280 2355
Size: 2280 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1153 2355
Size: 1153 1202
BstBI TT'CG_AA
Cuts at: 0 1193 2355
Size: 1193 1162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 728 2239 2355
Size: 728 1511 116
Fragments arranged by size:
1511 728 116
BstYI r'GATC_y
Cuts at: 0 407 2355
Size: 407 1948
BstZ17I GTA'TAC
Cuts at: 0 1861 2355
Size: 1861 494
BtgI C'CryG_G
Cuts at: 0 1947 2355
Size: 1947 408
BtsI GCAGTG_nn'
Cuts at: 0 599 1434 2355
Size: 599 835 921
Fragments arranged by size:
921 835 599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1929 1964 2355
Size: 1929 35 391
Fragments arranged by size:
1929 391 35
DraI TTT'AAA
Cuts at: 0 2103 2355
Size: 2103 252
EaeI y'GGCC_r
Cuts at: 0 718 1775 2355
Size: 718 1057 580
Fragments arranged by size:
1057 718 580
EarI CTCTTCn'nnn_
Cuts at: 0 349 983 1040 1244 1691 2355
Size: 349 634 57 204 447 664
Fragments arranged by size:
664 634 447 349 204 57
EcoICRI GAG'CTC
Cuts at: 0 1975 2355
Size: 1975 380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 494 1460 2113 2271 2355
Size: 449 45 966 653 158 84
Fragments arranged by size:
966 653 449 158 84 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2047 2355
Size: 2047 308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1206 2355
Size: 1206 1149
HaeII r_GCGC'y
Cuts at: 0 16 64 2355
Size: 16 48 2291
Fragments arranged by size:
2291 48 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 392 424 456 958 990 1246
Size: 348 32 12 32 32 502 32 256
Cuts at: 1246 1278 1514 1546 1971 2003 2355
Size: 32 236 32 425 32 352
Fragments arranged by size:
502 425 352 348 256 236 32 32
32 32 32 32 32 12
HincII GTy'rAC
Cuts at: 0 492 2355
Size: 492 1863
HindIII A'AGCT_T
Cuts at: 0 566 755 2296 2355
Size: 566 189 1541 59
Fragments arranged by size:
1541 566 189 59
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 1054 1587 2355
Size: 523 531 533 768
Fragments arranged by size:
768 533 531 523
MscI TGG'CCA
Cuts at: 0 1777 2355
Size: 1777 578
MslI CAynn'nnrTG
Cuts at: 0 788 1643 2224 2237 2355
Size: 788 855 581 13 118
Fragments arranged by size:
855 788 581 118 13
NspI r_CATG'y
Cuts at: 0 151 2355
Size: 151 2204
PciI A'CATG_T
Cuts at: 0 147 2355
Size: 147 2208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2027 2059 2355
Size: 2027 32 296
Fragments arranged by size:
2027 296 32
PsiI TTA'TAA
Cuts at: 0 1822 2276 2355
Size: 1822 454 79
Fragments arranged by size:
1822 454 79
PstI C_TGCA'G
Cuts at: 0 2051 2355
Size: 2051 304
SacI G_AGCT'C
Cuts at: 0 1977 2355
Size: 1977 378
SbfI CC_TGCA'GG
Cuts at: 0 2051 2355
Size: 2051 304
ScaI AGT'ACT
Cuts at: 0 835 2355
Size: 835 1520
SfcI C'TryA_G
Cuts at: 0 674 1547 2047 2355
Size: 674 873 500 308
Fragments arranged by size:
873 674 500 308
SmlI C'TyrA_G
Cuts at: 0 1180 1895 2355
Size: 1180 715 460
Fragments arranged by size:
1180 715 460
SspI AAT'ATT
Cuts at: 0 638 2355
Size: 638 1717
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1799 2355
Size: 1799 556
TatI w'GTAC_w
Cuts at: 0 125 833 2280 2355
Size: 125 708 1447 75
Fragments arranged by size:
1447 708 125 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 597 629 1263 1295 2355
Size: 597 32 634 32 1060
Fragments arranged by size:
1060 634 597 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1189 2138 2355
Size: 1189 949 217
Fragments arranged by size:
1189 949 217
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaLI
ApoI BanII BbsI BbvCI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrGI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtsI CspCI DraI EaeI EarI
EcoICRI Eco57MI EcoNI FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI NspI PciI PpiI PsiI PstI
SacI SbfI ScaI SfcI SmlI SspI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstEII Bsu36I BtgZI ClaI DraIII DrdI EagI EciI
EcoO109I EcoRI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacII SalI SanDI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI