Restriction map for
pF1KB5714
(Linear) (Six-base) MAPSORT of: KIBB5714.seq Check: 7958 from: 1 to: 2163
KIBB5714.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1637 2163
Size: 1637 526
AccI GT'mk_AC
Cuts at: 0 314 2163
Size: 314 1849
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 173 2163
Size: 173 1990
AlwNI CAG_nnn'CTG
Cuts at: 0 1008 1041 2163
Size: 1008 33 1122
Fragments arranged by size:
1122 1008 33
ApoI r'AATT_y
Cuts at: 0 1262 1976 2163
Size: 1262 714 187
Fragments arranged by size:
1262 714 187
BanII G_rGCy'C
Cuts at: 0 181 2163
Size: 181 1982
BbsI GAAGACnn'nnnn_
Cuts at: 0 162 1571 1739 2163
Size: 162 1409 168 424
Fragments arranged by size:
1409 424 168 162
BbvCI CC'TCA_GC
Cuts at: 0 1883 2163
Size: 1883 280
BclI T'GATC_A
Cuts at: 0 1845 2081 2163
Size: 1845 236 82
Fragments arranged by size:
1845 236 82
Bme1580I G_kGCm'C
Cuts at: 0 1521 2163
Size: 1521 642
BmrI ACTGGGnnnn_n'
Cuts at: 0 836 1395 2163
Size: 836 559 768
Fragments arranged by size:
836 768 559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 726 1296 2163
Size: 497 229 570 867
Fragments arranged by size:
867 570 497 229
Bpu10I CC'TnA_GC
Cuts at: 0 254 1883 2163
Size: 254 1629 280
Fragments arranged by size:
1629 280 254
BsaI GGTCTCn'nnnn_
Cuts at: 0 1174 2163
Size: 1174 989
BsaAI yAC'GTr
Cuts at: 0 1284 2163
Size: 1284 879
BsaBI GATnn'nnATC
Cuts at: 0 1115 1805 2163
Size: 1115 690 358
Fragments arranged by size:
1115 690 358
BsaWI w'CCGG_w
Cuts at: 0 890 2163
Size: 890 1273
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 470 500 1294 1324 2163
Size: 470 30 794 30 839
Fragments arranged by size:
839 794 470 30 30
BseYI C'CCAG_C
Cuts at: 0 372 1131 1294 2163
Size: 372 759 163 869
Fragments arranged by size:
869 759 372 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1348 2163
Size: 1016 332 815
Fragments arranged by size:
1016 815 332
BsiEI CG_ry'CG
Cuts at: 0 98 2163
Size: 98 2065
BsiHKAI G_wGCw'C
Cuts at: 0 429 2163
Size: 429 1734
BsmI GAATG_Cn'
Cuts at: 0 737 1298 1375 1587 2163
Size: 737 561 77 212 576
Fragments arranged by size:
737 576 561 212 77
Bsp1286I G_dGCh'C
Cuts at: 0 181 429 1521 2163
Size: 181 248 1092 642
Fragments arranged by size:
1092 642 248 181
BspHI T'CATG_A
Cuts at: 0 444 2163
Size: 444 1719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 959 1637 2035 2163
Size: 959 678 398 128
Fragments arranged by size:
959 678 398 128
BsrDI GCAATG_nn'
Cuts at: 0 1505 2163
Size: 1505 658
BsrFI r'CCGG_y
Cuts at: 0 868 2163
Size: 868 1295
BstBI TT'CG_AA
Cuts at: 0 2106 2163
Size: 2106 57
BstYI r'GATC_y
Cuts at: 0 544 1359 2163
Size: 544 815 804
Fragments arranged by size:
815 804 544
BtsI GCAGTG_nn'
Cuts at: 0 2084 2163
Size: 2084 79
EaeI y'GGCC_r
Cuts at: 0 56 95 900 1088 1623 2163
Size: 56 39 805 188 535 540
Fragments arranged by size:
805 540 535 188 56 39
EagI C'GGCC_G
Cuts at: 0 95 2163
Size: 95 2068
EarI CTCTTCn'nnn_
Cuts at: 0 206 1915 2163
Size: 206 1709 248
Fragments arranged by size:
1709 248 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 726 1296 2163
Size: 497 229 570 867
Fragments arranged by size:
867 570 497 229
EcoO109I rG'GnC_Cy
Cuts at: 0 1005 2163
Size: 1005 1158
EcoRV GAT'ATC
Cuts at: 0 561 1698 2163
Size: 561 1137 465
Fragments arranged by size:
1137 561 465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 553 2163
Size: 553 1610
HaeII r_GCGC'y
Cuts at: 0 69 2163
Size: 69 2094
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 679 711 2163
Size: 679 32 1452
Fragments arranged by size:
1452 679 32
HincII GTy'rAC
Cuts at: 0 1497 2163
Size: 1497 666
HpaI GTT'AAC
Cuts at: 0 1497 2163
Size: 1497 666
MfeI C'AATT_G
Cuts at: 0 365 420 863 2163
Size: 365 55 443 1300
Fragments arranged by size:
1300 443 365 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 657 1857 2040 2163
Size: 522 135 1200 183 123
Fragments arranged by size:
1200 522 183 135 123
MscI TGG'CCA
Cuts at: 0 58 902 1090 2163
Size: 58 844 188 1073
Fragments arranged by size:
1073 844 188 58
MslI CAynn'nnrTG
Cuts at: 0 155 2143 2163
Size: 155 1988 20
Fragments arranged by size:
1988 155 20
MspA1I CmG'CkG
Cuts at: 0 227 2163
Size: 227 1936
NotI GC'GGCC_GC
Cuts at: 0 95 2163
Size: 95 2068
NspI r_CATG'y
Cuts at: 0 154 2163
Size: 154 2009
PflMI CCAn_nnn'nTGG
Cuts at: 0 1710 2163
Size: 1710 453
PmlI CAC'GTG
Cuts at: 0 1284 2163
Size: 1284 879
PpuMI rG'GwC_Cy
Cuts at: 0 1005 2163
Size: 1005 1158
PstI C_TGCA'G
Cuts at: 0 240 2163
Size: 240 1923
PvuII CAG'CTG
Cuts at: 0 227 2163
Size: 227 1936
SexAI A'CCwGG_T
Cuts at: 0 2075 2163
Size: 2075 88
SfcI C'TryA_G
Cuts at: 0 236 336 2163
Size: 236 100 1827
Fragments arranged by size:
1827 236 100
StyI C'CwwG_G
Cuts at: 0 494 1076 2163
Size: 494 582 1087
Fragments arranged by size:
1087 582 494
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 836 1414 1531 2163
Size: 118 718 578 117 632
Fragments arranged by size:
718 632 578 118 117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 2163
Size: 1294 32 837
Fragments arranged by size:
1294 837 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1083 2163
Size: 1083 1080
XmnI GAAnn'nnTTC
Cuts at: 0 1375 1736 2163
Size: 1375 361 427
Fragments arranged by size:
1375 427 361
Enzymes that do cut:
AarI AccI AlfI AlwNI ApoI BanII BbsI BbvCI
BclI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrFI BstBI BstYI BtsI EaeI
EagI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII HpaI MfeI MmeI MscI MslI MspA1I NotI
NspI PflMI PmlI PpuMI PstI PvuII SexAI SfcI
StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI
BsaHI BseRI BsiWI BsmBI BspEI BsrBI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HindIII KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI ZraI