Restriction map for
pF1KB5730
(Linear) (Six-base) MAPSORT of: KIBB5730.seq Check: 2 from: 1 to: 2196
KIBB5730.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 40 1416 2196
Size: 40 1376 780
Fragments arranged by size:
1376 780 40
AatII G_ACGT'C
Cuts at: 0 1616 2196
Size: 1616 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 603 1427 1477 1852 2196
Size: 294 309 824 50 375 344
Fragments arranged by size:
824 375 344 309 294 50
AflIII A'CryG_T
Cuts at: 0 1034 2196
Size: 1034 1162
AhdI GACnn_n'nnGTC
Cuts at: 0 948 2196
Size: 948 1248
AleI CACnn'nnGTG
Cuts at: 0 286 2196
Size: 286 1910
AlwNI CAG_nnn'CTG
Cuts at: 0 427 496 855 1173 2183 2196
Size: 427 69 359 318 1010 13
Fragments arranged by size:
1010 427 359 318 69 13
ApaI G_GGCC'C
Cuts at: 0 1455 2111 2196
Size: 1455 656 85
Fragments arranged by size:
1455 656 85
ApaLI G'TGCA_C
Cuts at: 0 2150 2196
Size: 2150 46
ApoI r'AATT_y
Cuts at: 0 408 2196
Size: 408 1788
AvaI C'yCGr_G
Cuts at: 0 1296 2196
Size: 1296 900
AvrII C'CTAG_G
Cuts at: 0 630 2196
Size: 630 1566
BanI G'GyrC_C
Cuts at: 0 416 1964 2196
Size: 416 1548 232
Fragments arranged by size:
1548 416 232
BanII G_rGCy'C
Cuts at: 0 991 1259 1455 2111 2196
Size: 991 268 196 656 85
Fragments arranged by size:
991 656 268 196 85
BbvCI CC'TCA_GC
Cuts at: 0 1999 2113 2196
Size: 1999 114 83
Fragments arranged by size:
1999 114 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1233 1267 1494 1528 2196
Size: 1233 34 227 34 668
Fragments arranged by size:
1233 668 227 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 462 2196
Size: 462 1734
BglII A'GATC_T
Cuts at: 0 722 734 2012 2196
Size: 722 12 1278 184
Fragments arranged by size:
1278 722 184 12
BlpI GC'TnA_GC
Cuts at: 0 1162 2196
Size: 1162 1034
Bme1580I G_kGCm'C
Cuts at: 0 882 1455 2111 2154 2196
Size: 882 573 656 43 42
Fragments arranged by size:
882 656 573 43 42
BmgBI CAC'GTC
Cuts at: 0 1017 2196
Size: 1017 1179
BmtI G_CTAG'C
Cuts at: 0 1927 2196
Size: 1927 269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1010 2196
Size: 1010 1186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 90 418 886 1106 1795 2160 2196
Size: 71 19 328 468 220 689 365 36
Fragments arranged by size:
689 468 365 328 220 71 36 19
Bpu10I CC'TnA_GC
Cuts at: 0 985 1525 1999 2113 2196
Size: 985 540 474 114 83
Fragments arranged by size:
985 540 474 114 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 904 2196
Size: 685 219 1292
Fragments arranged by size:
1292 685 219
BsaI GGTCTCn'nnnn_
Cuts at: 0 331 782 1381 2196
Size: 331 451 599 815
Fragments arranged by size:
815 599 451 331
BsaAI yAC'GTr
Cuts at: 0 2155 2196
Size: 2155 41
BsaHI Gr'CG_yC
Cuts at: 0 1613 2196
Size: 1613 583
BsaWI w'CCGG_w
Cuts at: 0 1535 2196
Size: 1535 661
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1104 1134 2196
Size: 1104 30 1062
Fragments arranged by size:
1104 1062 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 535 800 1066 1249 1399 1657 2196
Size: 448 87 265 266 183 150 258 539
Fragments arranged by size:
539 448 266 265 258 183 150 87
BseYI C'CCAG_C
Cuts at: 0 768 780 1987 2006 2053 2124 2196
Size: 768 12 1207 19 47 71 72
Fragments arranged by size:
1207 768 72 71 47 19 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 2196
Size: 282 1914
BsiHKAI G_wGCw'C
Cuts at: 0 85 1303 2154 2163 2196
Size: 85 1218 851 9 33
Fragments arranged by size:
1218 851 85 33 9
BsmI GAATG_Cn'
Cuts at: 0 1908 2196
Size: 1908 288
Bsp1286I G_dGCh'C
Cuts at: 0 85 882 991 1259 1303 1455 2111 2154
Size: 85 797 109 268 44 152 656 43
Cuts at: 2154 2163 2196
Size: 9 33
Fragments arranged by size:
797 656 268 152 109 85 44 43
33 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 1416 2196
Size: 40 1376 780
Fragments arranged by size:
1376 780 40
BssSI C'ACGA_G
Cuts at: 0 1003 1741 2196
Size: 1003 738 455
Fragments arranged by size:
1003 738 455
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2158 2196
Size: 2158 38
BstBI TT'CG_AA
Cuts at: 0 2022 2196
Size: 2022 174
BstEII G'GTnAC_C
Cuts at: 0 1110 2196
Size: 1110 1086
BstXI CCAn_nnnn'nTGG
Cuts at: 0 52 214 2196
Size: 52 162 1982
Fragments arranged by size:
1982 162 52
BstYI r'GATC_y
Cuts at: 0 249 722 734 2012 2196
Size: 249 473 12 1278 184
Fragments arranged by size:
1278 473 249 184 12
BtgI C'CryG_G
Cuts at: 0 104 121 1968 2103 2196
Size: 104 17 1847 135 93
Fragments arranged by size:
1847 135 104 93 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1914 2196
Size: 1914 282
BtsI GCAGTG_nn'
Cuts at: 0 134 1328 2196
Size: 134 1194 868
Fragments arranged by size:
1194 868 134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1573 1608 2196
Size: 1573 35 588
Fragments arranged by size:
1573 588 35
DraIII CAC_nnn'GTG
Cuts at: 0 2158 2196
Size: 2158 38
EaeI y'GGCC_r
Cuts at: 0 131 603 2196
Size: 131 472 1593
Fragments arranged by size:
1593 472 131
EarI CTCTTCn'nnn_
Cuts at: 0 20 86 819 898 2196
Size: 20 66 733 79 1298
Fragments arranged by size:
1298 733 79 66 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 90 294 418 603 886 1106 1427
Size: 71 19 204 124 185 283 220 321
Cuts at: 1427 1477 1795 1852 2160 2196
Size: 50 318 57 308 36
Fragments arranged by size:
321 318 308 283 220 204 185 124
71 57 50 36 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 324 2133 2196
Size: 324 1809 63
Fragments arranged by size:
1809 324 63
EcoO109I rG'GnC_Cy
Cuts at: 0 634 860 1452 2108 2196
Size: 634 226 592 656 88
Fragments arranged by size:
656 634 592 226 88
EcoRI G'AATT_C
Cuts at: 0 408 2196
Size: 408 1788
HaeII r_GCGC'y
Cuts at: 0 1721 1839 2196
Size: 1721 118 357
Fragments arranged by size:
1721 357 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 934 966 1104 1136 2196
Size: 934 32 138 32 1060
Fragments arranged by size:
1060 934 138 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1695 2196
Size: 1695 501
MscI TGG'CCA
Cuts at: 0 133 605 2196
Size: 133 472 1591
Fragments arranged by size:
1591 472 133
MslI CAynn'nnrTG
Cuts at: 0 240 286 976 2196
Size: 240 46 690 1220
Fragments arranged by size:
1220 690 240 46
MspA1I CmG'CkG
Cuts at: 0 768 873 1660 2147 2183 2196
Size: 768 105 787 487 36 13
Fragments arranged by size:
787 768 487 105 36 13
NcoI C'CATG_G
Cuts at: 0 121 1968 2196
Size: 121 1847 228
Fragments arranged by size:
1847 228 121
NheI G'CTAG_C
Cuts at: 0 1923 2196
Size: 1923 273
NspI r_CATG'y
Cuts at: 0 1038 2196
Size: 1038 1158
PciI A'CATG_T
Cuts at: 0 1034 2196
Size: 1034 1162
PflMI CCAn_nnn'nTGG
Cuts at: 0 427 944 1017 1398 2196
Size: 427 517 73 381 798
Fragments arranged by size:
798 517 427 381 73
PfoI T'CCnGG_A
Cuts at: 0 254 1142 1337 1811 2196
Size: 254 888 195 474 385
Fragments arranged by size:
888 474 385 254 195
PmlI CAC'GTG
Cuts at: 0 2155 2196
Size: 2155 41
PpuMI rG'GwC_Cy
Cuts at: 0 634 2196
Size: 634 1562
PspOMI G'GGCC_C
Cuts at: 0 1451 2107 2196
Size: 1451 656 89
Fragments arranged by size:
1451 656 89
PstI C_TGCA'G
Cuts at: 0 492 1085 1427 1517 1911 1940 2196
Size: 492 593 342 90 394 29 256
Fragments arranged by size:
593 492 394 342 256 90 29
PvuII CAG'CTG
Cuts at: 0 768 1660 2196
Size: 768 892 536
Fragments arranged by size:
892 768 536
SapI GCTCTTCn'nnn_
Cuts at: 0 20 898 2196
Size: 20 878 1298
Fragments arranged by size:
1298 878 20
SfcI C'TryA_G
Cuts at: 0 488 1081 1423 1513 1907 1936 2196
Size: 488 593 342 90 394 29 260
Fragments arranged by size:
593 488 394 342 260 90 29
SmlI C'TyrA_G
Cuts at: 0 700 919 2196
Size: 700 219 1277
Fragments arranged by size:
1277 700 219
SspI AAT'ATT
Cuts at: 0 1024 2196
Size: 1024 1172
StuI AGG'CCT
Cuts at: 0 1288 2196
Size: 1288 908
StyI C'CwwG_G
Cuts at: 0 121 630 1606 1968 2196
Size: 121 509 976 362 228
Fragments arranged by size:
976 509 362 228 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 1212 2196
Size: 434 778 984
Fragments arranged by size:
984 778 434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 650 682 830 862 2196
Size: 270 32 348 32 148 32 1334
Fragments arranged by size:
1334 348 270 148 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 442 1324 2196
Size: 442 882 872
Fragments arranged by size:
882 872 442
ZraI GAC'GTC
Cuts at: 0 1614 2196
Size: 1614 582
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BbvCI BcgI
BciVI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NheI NspI
PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SapI SfcI SmlI SspI StuI StyI TaqII TstI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AlfI AloI
AscI AseI AsiSI BaeI BamHI BbeI BbsI BclI
BfrBI BglI BmrI BsaBI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstZ17I Bsu36I
ClaI DraI DrdI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PasI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI