Restriction map for
pF1KB5736
(Linear) (Six-base) MAPSORT of: KIBB5736.seq Check: 3657 from: 1 to: 2208
KIBB5736.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 945 2208
Size: 945 1263
AatII G_ACGT'C
Cuts at: 0 751 2208
Size: 751 1457
AccI GT'mk_AC
Cuts at: 0 1780 2208
Size: 1780 428
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 1574 2208
Size: 741 833 634
Fragments arranged by size:
833 741 634
AflII C'TTAA_G
Cuts at: 0 697 2208
Size: 697 1511
AhdI GACnn_n'nnGTC
Cuts at: 0 1312 1471 2208
Size: 1312 159 737
Fragments arranged by size:
1312 737 159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 698 2208
Size: 698 1510
AlwNI CAG_nnn'CTG
Cuts at: 0 1303 2208
Size: 1303 905
ApaI G_GGCC'C
Cuts at: 0 1833 1933 2103 2208
Size: 1833 100 170 105
Fragments arranged by size:
1833 170 105 100
ApaLI G'TGCA_C
Cuts at: 0 1021 2208
Size: 1021 1187
ApoI r'AATT_y
Cuts at: 0 209 2208
Size: 209 1999
AvaI C'yCGr_G
Cuts at: 0 289 317 364 1819 1834 2208
Size: 289 28 47 1455 15 374
Fragments arranged by size:
1455 374 289 47 28 15
BamHI G'GATC_C
Cuts at: 0 1065 2208
Size: 1065 1143
BanI G'GyrC_C
Cuts at: 0 154 330 1162 1960 2208
Size: 154 176 832 798 248
Fragments arranged by size:
832 798 248 176 154
BanII G_rGCy'C
Cuts at: 0 583 1109 1366 1833 1871 1933 1980 1991
Size: 583 526 257 467 38 62 47 11
Cuts at: 1991 2103 2125 2208
Size: 112 22 83
Fragments arranged by size:
583 526 467 257 112 83 62 47
38 22 11
BbeI G_GCGC'C
Cuts at: 0 158 334 2208
Size: 158 176 1874
Fragments arranged by size:
1874 176 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 1503 2208
Size: 1503 705
BbvCI CC'TCA_GC
Cuts at: 0 1009 2208
Size: 1009 1199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 99 133 2208
Size: 99 34 2075
Fragments arranged by size:
2075 99 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 267 565 1593 2208
Size: 267 298 1028 615
Fragments arranged by size:
1028 615 298 267
BglI GCCn_nnn'nGGC
Cuts at: 0 1883 2208
Size: 1883 325
Bme1580I G_kGCm'C
Cuts at: 0 1025 1833 1933 1965 2103 2132 2208
Size: 1025 808 100 32 138 29 76
Fragments arranged by size:
1025 808 138 100 76 32 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 1964 2208
Size: 26 1938 244
Fragments arranged by size:
1938 244 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 600 2208
Size: 600 1608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 320 460 574 628 760 1280 1563
Size: 287 33 140 114 54 132 520 283
Cuts at: 1563 1857 2054 2063 2090 2208
Size: 294 197 9 27 118
Fragments arranged by size:
520 294 287 283 197 140 132 118
114 54 33 27 9
Bpu10I CC'TnA_GC
Cuts at: 0 1009 1409 2208
Size: 1009 400 799
Fragments arranged by size:
1009 799 400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 531 1318 2208
Size: 375 156 787 890
Fragments arranged by size:
890 787 375 156
BsaI GGTCTCn'nnnn_
Cuts at: 0 1252 1511 2108 2208
Size: 1252 259 597 100
Fragments arranged by size:
1252 597 259 100
BsaAI yAC'GTr
Cuts at: 0 1395 2208
Size: 1395 813
BsaBI GATnn'nnATC
Cuts at: 0 1342 2208
Size: 1342 866
BsaHI Gr'CG_yC
Cuts at: 0 155 331 748 2208
Size: 155 176 417 1460
Fragments arranged by size:
1460 417 176 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 733 763 874 904 1561 1591
Size: 565 30 138 30 111 30 657 30
Cuts at: 1591 1593 1623 2208
Size: 2 30 585
Fragments arranged by size:
657 585 565 138 111 30 30 30
30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 573 652 1064 1179 1601 1837 1925 2208
Size: 573 79 412 115 422 236 88 283
Fragments arranged by size:
573 422 412 283 236 115 88 79
BseYI C'CCAG_C
Cuts at: 0 520 1061 1170 2182 2208
Size: 520 541 109 1012 26
Fragments arranged by size:
1012 541 520 109 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2107 2208
Size: 2107 101
BsiEI CG_ry'CG
Cuts at: 0 920 2208
Size: 920 1288
BsiHKAI G_wGCw'C
Cuts at: 0 583 1025 1201 2208
Size: 583 442 176 1007
Fragments arranged by size:
1007 583 442 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 31 863 2208
Size: 31 832 1345
Fragments arranged by size:
1345 832 31
Bsp1286I G_dGCh'C
Cuts at: 0 583 1025 1109 1201 1366 1833 1871 1933
Size: 583 442 84 92 165 467 38 62
Cuts at: 1933 1965 1980 1991 2103 2125 2132 2208
Size: 32 15 11 112 22 7 76
Fragments arranged by size:
583 467 442 165 112 92 84 76
62 38 32 22 15 11 7
BspHI T'CATG_A
Cuts at: 0 593 896 2048 2208
Size: 593 303 1152 160
Fragments arranged by size:
1152 593 303 160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 644 945 2208
Size: 644 301 1263
Fragments arranged by size:
1263 644 301
BsrBI CCG'CTC
Cuts at: 0 486 864 1413 1827 2208
Size: 486 378 549 414 381
Fragments arranged by size:
549 486 414 381 378
BsrDI GCAATG_nn'
Cuts at: 0 864 2208
Size: 864 1344
BsrFI r'CCGG_y
Cuts at: 0 707 2208
Size: 707 1501
BstEII G'GTnAC_C
Cuts at: 0 1275 2208
Size: 1275 933
BstXI CCAn_nnnn'nTGG
Cuts at: 0 535 606 1287 1999 2043 2208
Size: 535 71 681 712 44 165
Fragments arranged by size:
712 681 535 165 71 44
BstYI r'GATC_y
Cuts at: 0 89 1065 2208
Size: 89 976 1143
Fragments arranged by size:
1143 976 89
Bsu36I CC'TnA_GG
Cuts at: 0 1076 2208
Size: 1076 1132
BtgI C'CryG_G
Cuts at: 0 149 668 1148 1286 2171 2208
Size: 149 519 480 138 885 37
Fragments arranged by size:
885 519 480 149 138 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1937 2208
Size: 1937 271
BtsI GCAGTG_nn'
Cuts at: 0 513 687 1504 1743 1779 1857 1863 2090
Size: 513 174 817 239 36 78 6 227
Cuts at: 2090 2208
Size: 118
Fragments arranged by size:
817 513 239 227 174 118 78 36
6
DraIII CAC_nnn'GTG
Cuts at: 0 1789 2208
Size: 1789 419
EaeI y'GGCC_r
Cuts at: 0 566 849 917 1052 2168 2208
Size: 566 283 68 135 1116 40
Fragments arranged by size:
1116 566 283 135 68 40
EagI C'GGCC_G
Cuts at: 0 917 2208
Size: 917 1291
EarI CTCTTCn'nnn_
Cuts at: 0 62 2208
Size: 62 2146
EcoICRI GAG'CTC
Cuts at: 0 581 2208
Size: 581 1627
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 320 460 574 628 741 760 1280
Size: 287 33 140 114 54 113 19 520
Cuts at: 1280 1563 1574 1857 2054 2063 2090 2208
Size: 283 11 283 197 9 27 118
Fragments arranged by size:
520 287 283 283 197 140 118 114
113 54 33 27 19 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 2208
Size: 61 2147
EcoO109I rG'GnC_Cy
Cuts at: 0 1830 1929 1930 2099 2100 2208
Size: 1830 99 1 169 1 108
Fragments arranged by size:
1830 169 108 99 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 843 1503 2208
Size: 843 660 705
Fragments arranged by size:
843 705 660
HaeII r_GCGC'y
Cuts at: 0 158 334 521 561 705 2208
Size: 158 176 187 40 144 1503
Fragments arranged by size:
1503 187 176 158 144 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 285 317 603 635 733 765 1255 1287
Size: 285 32 286 32 98 32 490 32
Cuts at: 1287 1298 1330 1457 1489 1699 1731 1828 1860
Size: 11 32 127 32 210 32 97 32
Cuts at: 1860 2107 2139 2208
Size: 247 32 69
Fragments arranged by size:
490 286 285 247 210 127 98 97
69 32 32 32 32 32 32 32
32 32 11
HincII GTy'rAC
Cuts at: 0 1377 1476 1551 1781 2208
Size: 1377 99 75 230 427
Fragments arranged by size:
1377 427 230 99 75
HindIII A'AGCT_T
Cuts at: 0 2157 2208
Size: 2157 51
KasI G'GCGC_C
Cuts at: 0 154 330 2208
Size: 154 176 1878
Fragments arranged by size:
1878 176 154
MfeI C'AATT_G
Cuts at: 0 1118 2208
Size: 1118 1090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1488 2208
Size: 1488 720
MscI TGG'CCA
Cuts at: 0 851 1054 2170 2208
Size: 851 203 1116 38
Fragments arranged by size:
1116 851 203 38
MslI CAynn'nnrTG
Cuts at: 0 59 413 1997 2041 2170 2208
Size: 59 354 1584 44 129 38
Fragments arranged by size:
1584 354 129 59 44 38
MspA1I CmG'CkG
Cuts at: 0 144 151 377 1150 1439 2208
Size: 144 7 226 773 289 769
Fragments arranged by size:
773 769 289 226 144 7
NarI GG'CG_CC
Cuts at: 0 155 331 2208
Size: 155 176 1877
Fragments arranged by size:
1877 176 155
NcoI C'CATG_G
Cuts at: 0 1286 2171 2208
Size: 1286 885 37
Fragments arranged by size:
1286 885 37
NspI r_CATG'y
Cuts at: 0 948 1329 2208
Size: 948 381 879
Fragments arranged by size:
948 879 381
PasI CC'CwG_GG
Cuts at: 0 1984 2208
Size: 1984 224
PflMI CCAn_nnn'nTGG
Cuts at: 0 1061 1303 1395 1631 2208
Size: 1061 242 92 236 577
Fragments arranged by size:
1061 577 242 236 92
PfoI T'CCnGG_A
Cuts at: 0 1316 2208
Size: 1316 892
PmlI CAC'GTG
Cuts at: 0 1395 2208
Size: 1395 813
PspOMI G'GGCC_C
Cuts at: 0 1829 1929 2099 2208
Size: 1829 100 170 109
Fragments arranged by size:
1829 170 109 100
PstI C_TGCA'G
Cuts at: 0 143 2208
Size: 143 2065
SacI G_AGCT'C
Cuts at: 0 583 2208
Size: 583 1625
SacII CC_GC'GG
Cuts at: 0 152 1151 2208
Size: 152 999 1057
Fragments arranged by size:
1057 999 152
SalI G'TCGA_C
Cuts at: 0 1779 2208
Size: 1779 429
ScaI AGT'ACT
Cuts at: 0 163 2208
Size: 163 2045
SexAI A'CCwGG_T
Cuts at: 0 80 2208
Size: 80 2128
SfcI C'TryA_G
Cuts at: 0 139 1680 2208
Size: 139 1541 528
Fragments arranged by size:
1541 528 139
SfoI GGC'GCC
Cuts at: 0 156 332 2208
Size: 156 176 1876
Fragments arranged by size:
1876 176 156
SgrAI Cr'CCGG_yG
Cuts at: 0 707 2208
Size: 707 1501
SmlI C'TyrA_G
Cuts at: 0 390 546 697 1333 2208
Size: 390 156 151 636 875
Fragments arranged by size:
875 636 390 156 151
StuI AGG'CCT
Cuts at: 0 396 1081 1457 2208
Size: 396 685 376 751
Fragments arranged by size:
751 685 396 376
StyI C'CwwG_G
Cuts at: 0 1280 1286 2171 2208
Size: 1280 6 885 37
Fragments arranged by size:
1280 885 37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1125 1997 2208
Size: 1125 872 211
Fragments arranged by size:
1125 872 211
TatI w'GTAC_w
Cuts at: 0 161 821 2208
Size: 161 660 1387
Fragments arranged by size:
1387 660 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1312 1344 2057 2089 2208
Size: 1312 32 713 32 119
Fragments arranged by size:
1312 713 119 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 535 2208
Size: 535 1673
ZraI GAC'GTC
Cuts at: 0 749 2208
Size: 749 1459
Enzymes that do cut:
AarI AatII AccI AcuI AflII AhdI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PmlI PspOMI PstI SacI
SacII SalI ScaI SexAI SfcI SfoI SgrAI SmlI
StuI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AleI AloI AscI
AseI AsiSI AvrII BaeI BclI BfrBI BglII BlpI
BmgBI BmtI BsaWI BsiWI BsmI BspEI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I ClaI CspCI DraI DrdI
EciI EcoRI EcoRV FseI FspI FspAI HpaI KpnI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SanDI SapI SbfI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI