Restriction map for
pF1KB5737
(Linear) (Six-base) MAPSORT of: KIBB5737.seq Check: 3242 from: 1 to: 2385
KIBB5737.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 581 1214 2385
Size: 581 633 1171
Fragments arranged by size:
1171 633 581
Acc65I G'GTAC_C
Cuts at: 0 1330 2385
Size: 1330 1055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 707 1003 1498 1507 2055 2385
Size: 383 324 296 495 9 548 330
Fragments arranged by size:
548 495 383 330 324 296 9
AfeI AGC'GCT
Cuts at: 0 1249 2385
Size: 1249 1136
AleI CACnn'nnGTG
Cuts at: 0 889 1615 2385
Size: 889 726 770
Fragments arranged by size:
889 770 726
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 986 1018 2385
Size: 986 32 1367
Fragments arranged by size:
1367 986 32
AlwNI CAG_nnn'CTG
Cuts at: 0 272 1308 1476 1524 1666 2181 2385
Size: 272 1036 168 48 142 515 204
Fragments arranged by size:
1036 515 272 204 168 142 48
ApaI G_GGCC'C
Cuts at: 0 73 1203 2018 2385
Size: 73 1130 815 367
Fragments arranged by size:
1130 815 367 73
ApaLI G'TGCA_C
Cuts at: 0 1618 2385
Size: 1618 767
AvaI C'yCGr_G
Cuts at: 0 48 109 1190 1196 2385
Size: 48 61 1081 6 1189
Fragments arranged by size:
1189 1081 61 48 6
BamHI G'GATC_C
Cuts at: 0 719 2385
Size: 719 1666
BanI G'GyrC_C
Cuts at: 0 357 555 1012 1101 1330 2001 2063 2385
Size: 357 198 457 89 229 671 62 322
Fragments arranged by size:
671 457 357 322 229 198 89 62
BanII G_rGCy'C
Cuts at: 0 73 177 991 1197 1203 2018 2385
Size: 73 104 814 206 6 815 367
Fragments arranged by size:
815 814 367 206 104 73 6
BbeI G_GCGC'C
Cuts at: 0 361 1016 2067 2385
Size: 361 655 1051 318
Fragments arranged by size:
1051 655 361 318
BbsI GAAGACnn'nnnn_
Cuts at: 0 1069 1416 2385
Size: 1069 347 969
Fragments arranged by size:
1069 969 347
BglI GCCn_nnn'nGGC
Cuts at: 0 1052 1093 2051 2256 2376 2385
Size: 1052 41 958 205 120 9
Fragments arranged by size:
1052 958 205 120 41 9
Bme1580I G_kGCm'C
Cuts at: 0 30 73 1106 1203 1622 1941 2018 2385
Size: 30 43 1033 97 419 319 77 367
Fragments arranged by size:
1033 419 367 319 97 77 43 30
BmgBI CAC'GTC
Cuts at: 0 570 930 1422 2385
Size: 570 360 492 963
Fragments arranged by size:
963 570 492 360
BmrI ACTGGGnnnn_n'
Cuts at: 0 718 1491 2385
Size: 718 773 894
Fragments arranged by size:
894 773 718
BmtI G_CTAG'C
Cuts at: 0 137 625 2385
Size: 137 488 1760
Fragments arranged by size:
1760 488 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 185 614 988 1774 2385
Size: 105 80 429 374 786 611
Fragments arranged by size:
786 611 429 374 105 80
Bpu10I CC'TnA_GC
Cuts at: 0 1097 2385
Size: 1097 1288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 2385
Size: 294 2091
BsaI GGTCTCn'nnnn_
Cuts at: 0 1785 1829 2323 2332 2385
Size: 1785 44 494 9 53
Fragments arranged by size:
1785 494 53 44 9
BsaHI Gr'CG_yC
Cuts at: 0 358 387 1013 1357 2064 2294 2385
Size: 358 29 626 344 707 230 91
Fragments arranged by size:
707 626 358 344 230 91 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 375 405 587 617 986 1016 1849 1879
Size: 375 30 182 30 369 30 833 30
Cuts at: 1879 2385
Size: 506
Fragments arranged by size:
833 506 375 369 182 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 396 1297 1300 2338 2341 2385
Size: 391 5 901 3 1038 3 44
Fragments arranged by size:
1038 901 391 44 5 3 3
BseYI C'CCAG_C
Cuts at: 0 1517 2342 2385
Size: 1517 825 43
Fragments arranged by size:
1517 825 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 571 1573 2385
Size: 559 12 1002 812
Fragments arranged by size:
1002 812 559 12
BsiEI CG_ry'CG
Cuts at: 0 61 124 2385
Size: 61 63 2261
Fragments arranged by size:
2261 63 61
BsiHKAI G_wGCw'C
Cuts at: 0 177 1392 1622 1760 2385
Size: 177 1215 230 138 625
Fragments arranged by size:
1215 625 230 177 138
BsmI GAATG_Cn'
Cuts at: 0 471 644 944 2385
Size: 471 173 300 1441
Fragments arranged by size:
1441 471 300 173
BsmBI CGTCTCn'nnnn_
Cuts at: 0 378 2385
Size: 378 2007
Bsp1286I G_dGCh'C
Cuts at: 0 30 73 177 991 1106 1197 1203 1392
Size: 30 43 104 814 115 91 6 189
Cuts at: 1392 1622 1760 1941 2018 2385
Size: 230 138 181 77 367
Fragments arranged by size:
814 367 230 189 181 138 115 104
91 77 43 30 6
BspHI T'CATG_A
Cuts at: 0 452 1719 2385
Size: 452 1267 666
Fragments arranged by size:
1267 666 452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 633 2385
Size: 593 40 1752
Fragments arranged by size:
1752 593 40
BsrBI CCG'CTC
Cuts at: 0 396 420 1872 2385
Size: 396 24 1452 513
Fragments arranged by size:
1452 513 396 24
BsrDI GCAATG_nn'
Cuts at: 0 1581 2385
Size: 1581 804
BsrFI r'CCGG_y
Cuts at: 0 1120 1415 2385
Size: 1120 295 970
Fragments arranged by size:
1120 970 295
BssSI C'ACGA_G
Cuts at: 0 514 2385
Size: 514 1871
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1666 2385
Size: 1666 719
BstEII G'GTnAC_C
Cuts at: 0 1959 2385
Size: 1959 426
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1083 2385
Size: 1083 1302
BstYI r'GATC_y
Cuts at: 0 719 1682 2385
Size: 719 963 703
Fragments arranged by size:
963 719 703
Bsu36I CC'TnA_GG
Cuts at: 0 1951 2030 2385
Size: 1951 79 355
Fragments arranged by size:
1951 355 79
BtgI C'CryG_G
Cuts at: 0 599 1088 1643 1979 2253 2385
Size: 599 489 555 336 274 132
Fragments arranged by size:
599 555 489 336 274 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 426 1129 1545 1953 2385
Size: 426 703 416 408 432
Fragments arranged by size:
703 432 426 416 408
BtsI GCAGTG_nn'
Cuts at: 0 791 1033 2385
Size: 791 242 1352
Fragments arranged by size:
1352 791 242
ClaI AT'CG_AT
Cuts at: 0 1220 2385
Size: 1220 1165
DrdI GACnn_nn'nnGTC
Cuts at: 0 1420 2385
Size: 1420 965
EaeI y'GGCC_r
Cuts at: 0 2 58 121 524 794 821 1073 1085
Size: 2 56 63 403 270 27 252 12
Cuts at: 1085 1118 2120 2385
Size: 33 1002 265
Fragments arranged by size:
1002 403 270 265 252 63 56 33
27 12 2
EagI C'GGCC_G
Cuts at: 0 58 121 2385
Size: 58 63 2264
Fragments arranged by size:
2264 63 58
EarI CTCTTCn'nnn_
Cuts at: 0 359 368 662 979 1474 1852 2330 2360
Size: 359 9 294 317 495 378 478 30
Cuts at: 2360 2385
Size: 25
Fragments arranged by size:
495 478 378 359 317 294 30 25
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 2385
Size: 22 2363
EcoICRI GAG'CTC
Cuts at: 0 175 2385
Size: 175 2210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 185 383 614 707 988 1003 1498
Size: 105 80 198 231 93 281 15 495
Cuts at: 1498 1507 1774 2055 2385
Size: 9 267 281 330
Fragments arranged by size:
495 330 281 281 267 231 198 105
93 80 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 1180 1690 1954 2385
Size: 157 1023 510 264 431
Fragments arranged by size:
1023 510 431 264 157
EcoO109I rG'GnC_Cy
Cuts at: 0 52 69 150 1020 1200 2043 2385
Size: 52 17 81 870 180 843 342
Fragments arranged by size:
870 843 342 180 81 52 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 691 2385
Size: 691 1694
FseI GG_CCGG'CC
Cuts at: 0 1124 2385
Size: 1124 1261
FspI TGC'GCA
Cuts at: 0 427 2385
Size: 427 1958
HaeII r_GCGC'y
Cuts at: 0 361 1016 1160 1251 2067 2182 2385
Size: 361 655 144 91 816 115 203
Fragments arranged by size:
816 655 361 203 144 115 91
HindIII A'AGCT_T
Cuts at: 0 673 2385
Size: 673 1712
KasI G'GCGC_C
Cuts at: 0 357 1012 2063 2385
Size: 357 655 1051 322
Fragments arranged by size:
1051 655 357 322
KpnI G_GTAC'C
Cuts at: 0 1334 2385
Size: 1334 1051
MfeI C'AATT_G
Cuts at: 0 190 2385
Size: 190 2195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 2385
Size: 1298 1087
MscI TGG'CCA
Cuts at: 0 526 796 1075 1087 2122 2385
Size: 526 270 279 12 1035 263
Fragments arranged by size:
1035 526 279 270 263 12
MslI CAynn'nnrTG
Cuts at: 0 889 1258 1615 2385
Size: 889 369 357 770
Fragments arranged by size:
889 770 369 357
MspA1I CmG'CkG
Cuts at: 0 63 482 685 1863 2385
Size: 63 419 203 1178 522
Fragments arranged by size:
1178 522 419 203 63
NaeI GCC'GGC
Cuts at: 0 1122 2385
Size: 1122 1263
NarI GG'CG_CC
Cuts at: 0 358 1013 2064 2385
Size: 358 655 1051 321
Fragments arranged by size:
1051 655 358 321
NcoI C'CATG_G
Cuts at: 0 1979 2253 2385
Size: 1979 274 132
Fragments arranged by size:
1979 274 132
NgoMIV G'CCGG_C
Cuts at: 0 1120 2385
Size: 1120 1265
NheI G'CTAG_C
Cuts at: 0 133 621 2385
Size: 133 488 1764
Fragments arranged by size:
1764 488 133
PasI CC'CwG_GG
Cuts at: 0 1809 1887 2385
Size: 1809 78 498
Fragments arranged by size:
1809 498 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 803 1308 1524 1737 2385
Size: 803 505 216 213 648
Fragments arranged by size:
803 648 505 216 213
PfoI T'CCnGG_A
Cuts at: 0 496 2385
Size: 496 1889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 986 1018 2385
Size: 986 32 1367
Fragments arranged by size:
1367 986 32
PpuMI rG'GwC_Cy
Cuts at: 0 52 150 1020 2385
Size: 52 98 870 1365
Fragments arranged by size:
1365 870 98 52
PspOMI G'GGCC_C
Cuts at: 0 69 1199 2014 2385
Size: 69 1130 815 371
Fragments arranged by size:
1130 815 371 69
PstI C_TGCA'G
Cuts at: 0 182 1448 2087 2156 2385
Size: 182 1266 639 69 229
Fragments arranged by size:
1266 639 229 182 69
PvuII CAG'CTG
Cuts at: 0 685 1863 2385
Size: 685 1178 522
Fragments arranged by size:
1178 685 522
SacI G_AGCT'C
Cuts at: 0 177 2385
Size: 177 2208
SapI GCTCTTCn'nnn_
Cuts at: 0 979 1474 2360 2385
Size: 979 495 886 25
Fragments arranged by size:
979 886 495 25
SfcI C'TryA_G
Cuts at: 0 178 1444 2083 2130 2152 2385
Size: 178 1266 639 47 22 233
Fragments arranged by size:
1266 639 233 178 47 22
SfoI GGC'GCC
Cuts at: 0 359 1014 2065 2385
Size: 359 655 1051 320
Fragments arranged by size:
1051 655 359 320
SmaI CCC'GGG
Cuts at: 0 50 111 1198 2385
Size: 50 61 1087 1187
Fragments arranged by size:
1187 1087 61 50
SmlI C'TyrA_G
Cuts at: 0 309 2385
Size: 309 2076
SrfI GCCC'GGGC
Cuts at: 0 1198 2385
Size: 1198 1187
SspI AAT'ATT
Cuts at: 0 654 2385
Size: 654 1731
StuI AGG'CCT
Cuts at: 0 141 1151 2081 2128 2385
Size: 141 1010 930 47 257
Fragments arranged by size:
1010 930 257 141 47
StyI C'CwwG_G
Cuts at: 0 527 710 1016 1076 1691 1736 1853 1979
Size: 527 183 306 60 615 45 117 126
Cuts at: 1979 2076 2123 2253 2385
Size: 97 47 130 132
Fragments arranged by size:
615 527 306 183 132 130 126 117
97 60 47 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2347 2385
Size: 2347 38
TatI w'GTAC_w
Cuts at: 0 764 2385
Size: 764 1621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 375 407 587 619 2385
Size: 375 32 180 32 1766
Fragments arranged by size:
1766 375 180 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 508 1342 1497 2385
Size: 508 834 155 888
Fragments arranged by size:
888 834 508 155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 80 269 2385
Size: 80 189 2116
Fragments arranged by size:
2116 189 80
XmaI C'CCGG_G
Cuts at: 0 48 109 1196 2385
Size: 48 61 1087 1189
Fragments arranged by size:
1189 1087 61 48
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AloI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BglI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI HaeII HindIII KasI
KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI PasI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SapI SfcI SfoI SmaI
SmlI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AlfI
ApoI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BplI BsaAI BsaBI
BsaWI BsiWI BspEI BsrGI BssHII BstBI BstZ17I CspCI
DraI DraIII EcoRI EcoRV FspAI Hin4I HincII HpaI
MluI NdeI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SwaI XbaI XhoI XmnI
ZraI