Restriction map for
pF1KB5738
(Linear) (Six-base) MAPSORT of: KIBB5738.seq Check: 4980 from: 1 to: 2220
KIBB5738.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 448 2220
Size: 448 1772
Acc65I G'GTAC_C
Cuts at: 0 1880 2034 2220
Size: 1880 154 186
Fragments arranged by size:
1880 186 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1186 1478 2220
Size: 239 947 292 742
Fragments arranged by size:
947 742 292 239
AflIII A'CryG_T
Cuts at: 0 603 2220
Size: 603 1617
AhdI GACnn_n'nnGTC
Cuts at: 0 1059 2220
Size: 1059 1161
AlwNI CAG_nnn'CTG
Cuts at: 0 1318 1364 2220
Size: 1318 46 856
Fragments arranged by size:
1318 856 46
ApoI r'AATT_y
Cuts at: 0 553 715 736 2047 2220
Size: 553 162 21 1311 173
Fragments arranged by size:
1311 553 173 162 21
AseI AT'TA_AT
Cuts at: 0 382 2220
Size: 382 1838
AvaI C'yCGr_G
Cuts at: 0 884 986 2220
Size: 884 102 1234
Fragments arranged by size:
1234 884 102
AvrII C'CTAG_G
Cuts at: 0 471 2220
Size: 471 1749
BanI G'GyrC_C
Cuts at: 0 780 1880 2034 2124 2220
Size: 780 1100 154 90 96
Fragments arranged by size:
1100 780 154 96 90
BbsI GAAGACnn'nnnn_
Cuts at: 0 1050 1307 1624 2220
Size: 1050 257 317 596
Fragments arranged by size:
1050 596 317 257
BciVI GTATCCnnnnn_n'
Cuts at: 0 420 2220
Size: 420 1800
BfrBI ATG'CAT
Cuts at: 0 564 1269 2220
Size: 564 705 951
Fragments arranged by size:
951 705 564
BglII A'GATC_T
Cuts at: 0 630 2220
Size: 630 1590
BmrI ACTGGGnnnn_n'
Cuts at: 0 1333 2220
Size: 1333 887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1115 1912 2220
Size: 1070 45 797 308
Fragments arranged by size:
1070 797 308 45
BsaAI yAC'GTr
Cuts at: 0 1797 2220
Size: 1797 423
BsaWI w'CCGG_w
Cuts at: 0 783 2220
Size: 783 1437
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 2220
Size: 107 30 2083
Fragments arranged by size:
2083 107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 128 1604 2220
Size: 58 70 1476 616
Fragments arranged by size:
1476 616 70 58
BsmI GAATG_Cn'
Cuts at: 0 708 1230 2220
Size: 708 522 990
Fragments arranged by size:
990 708 522
BspHI T'CATG_A
Cuts at: 0 417 1653 2220
Size: 417 1236 567
Fragments arranged by size:
1236 567 417
BsrGI T'GTAC_A
Cuts at: 0 1320 1404 2220
Size: 1320 84 816
Fragments arranged by size:
1320 816 84
BstEII G'GTnAC_C
Cuts at: 0 1179 2220
Size: 1179 1041
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1451 2220
Size: 1451 769
BstYI r'GATC_y
Cuts at: 0 295 630 2220
Size: 295 335 1590
Fragments arranged by size:
1590 335 295
Bsu36I CC'TnA_GG
Cuts at: 0 1175 2220
Size: 1175 1045
BtgI C'CryG_G
Cuts at: 0 1743 1899 2220
Size: 1743 156 321
Fragments arranged by size:
1743 321 156
EarI CTCTTCn'nnn_
Cuts at: 0 47 725 1815 2220
Size: 47 678 1090 405
Fragments arranged by size:
1090 678 405 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1186 1478 2220
Size: 239 947 292 742
Fragments arranged by size:
947 742 292 239
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2155 2220
Size: 2155 65
EcoO109I rG'GnC_Cy
Cuts at: 0 1594 2220
Size: 1594 626
EcoRI G'AATT_C
Cuts at: 0 553 2220
Size: 553 1667
EcoRV GAT'ATC
Cuts at: 0 81 1740 2220
Size: 81 1659 480
Fragments arranged by size:
1659 480 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1095 1686 2220
Size: 1095 591 534
Fragments arranged by size:
1095 591 534
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 982 1014 1045 1077 1085 1117
Size: 280 32 670 32 31 32 8 32
Cuts at: 1117 1149 1181 1438 1470 2220
Size: 32 32 257 32 750
Fragments arranged by size:
750 670 280 257 32 32 32 32
32 32 32 31 8
HincII GTy'rAC
Cuts at: 0 1064 1287 2220
Size: 1064 223 933
Fragments arranged by size:
1064 933 223
KpnI G_GTAC'C
Cuts at: 0 1884 2038 2220
Size: 1884 154 182
Fragments arranged by size:
1884 182 154
MfeI C'AATT_G
Cuts at: 0 308 2220
Size: 308 1912
MslI CAynn'nnrTG
Cuts at: 0 401 2220
Size: 401 1819
MspA1I CmG'CkG
Cuts at: 0 2205 2220
Size: 2205 15
NdeI CA'TA_TG
Cuts at: 0 285 662 1951 2220
Size: 285 377 1289 269
Fragments arranged by size:
1289 377 285 269
NsiI A_TGCA'T
Cuts at: 0 566 1271 2220
Size: 566 705 949
Fragments arranged by size:
949 705 566
NspI r_CATG'y
Cuts at: 0 607 1411 2220
Size: 607 804 809
Fragments arranged by size:
809 804 607
PciI A'CATG_T
Cuts at: 0 603 2220
Size: 603 1617
PflMI CCAn_nnn'nTGG
Cuts at: 0 757 2220
Size: 757 1463
PfoI T'CCnGG_A
Cuts at: 0 1038 1103 2220
Size: 1038 65 1117
Fragments arranged by size:
1117 1038 65
PpuMI rG'GwC_Cy
Cuts at: 0 1594 2220
Size: 1594 626
PsiI TTA'TAA
Cuts at: 0 1150 1762 1916 2220
Size: 1150 612 154 304
Fragments arranged by size:
1150 612 304 154
PstI C_TGCA'G
Cuts at: 0 138 2220
Size: 138 2082
PvuII CAG'CTG
Cuts at: 0 2205 2220
Size: 2205 15
SapI GCTCTTCn'nnn_
Cuts at: 0 1815 2220
Size: 1815 405
ScaI AGT'ACT
Cuts at: 0 1573 2220
Size: 1573 647
SexAI A'CCwGG_T
Cuts at: 0 1428 2220
Size: 1428 792
SfcI C'TryA_G
Cuts at: 0 134 1536 2220
Size: 134 1402 684
Fragments arranged by size:
1402 684 134
SmaI CCC'GGG
Cuts at: 0 886 988 2220
Size: 886 102 1232
Fragments arranged by size:
1232 886 102
SmlI C'TyrA_G
Cuts at: 0 1085 1130 1927 2220
Size: 1085 45 797 293
Fragments arranged by size:
1085 797 293 45
SnaBI TAC'GTA
Cuts at: 0 1797 2220
Size: 1797 423
StuI AGG'CCT
Cuts at: 0 460 2220
Size: 460 1760
StyI C'CwwG_G
Cuts at: 0 423 471 776 2220
Size: 423 48 305 1444
Fragments arranged by size:
1444 423 305 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 10 350 2220
Size: 10 340 1870
Fragments arranged by size:
1870 340 10
TatI w'GTAC_w
Cuts at: 0 123 1320 1404 1571 2220
Size: 123 1197 84 167 649
Fragments arranged by size:
1197 649 167 123 84
XmaI C'CCGG_G
Cuts at: 0 884 986 2220
Size: 884 102 1234
Fragments arranged by size:
1234 884 102
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AlwNI ApoI AseI
AvaI AvrII BanI BbsI BciVI BfrBI BglII BmrI
BpuEI BsaAI BsaWI BsaXI BseRI BsmI BspHI BsrGI
BstEII BstXI BstYI Bsu36I BtgI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I HincII KpnI MfeI
MslI MspA1I NdeI NsiI NspI PciI PflMI PfoI
PpuMI PsiI PstI PvuII SapI ScaI SexAI SfcI
SmaI SmlI SnaBI StuI StyI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI BaeI BamHI BanII
BbeI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaBI BsaHI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI FseI FspI FspAI HaeII HindIII
HpaI KasI MluI MmeI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI