Restriction map for
pF1KB5740
(Linear) (Six-base) MAPSORT of: KIBB5740.seq Check: 5628 from: 1 to: 2220
KIBB5740.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 294 1670 2220
Size: 294 1376 550
Fragments arranged by size:
1376 550 294
AatII G_ACGT'C
Cuts at: 0 1634 2220
Size: 1634 586
Acc65I G'GTAC_C
Cuts at: 0 1156 1826 2220
Size: 1156 670 394
Fragments arranged by size:
1156 670 394
AfeI AGC'GCT
Cuts at: 0 2146 2220
Size: 2146 74
AflIII A'CryG_T
Cuts at: 0 1778 2220
Size: 1778 442
AleI CACnn'nnGTG
Cuts at: 0 1799 2095 2220
Size: 1799 296 125
Fragments arranged by size:
1799 296 125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1286 1678 2113 2130 2220
Size: 1286 392 435 17 90
Fragments arranged by size:
1286 435 392 90 17
AlwNI CAG_nnn'CTG
Cuts at: 0 309 771 2133 2220
Size: 309 462 1362 87
Fragments arranged by size:
1362 462 309 87
ApaI G_GGCC'C
Cuts at: 0 103 352 428 1453 2220
Size: 103 249 76 1025 767
Fragments arranged by size:
1025 767 249 103 76
ApaLI G'TGCA_C
Cuts at: 0 417 2220
Size: 417 1803
AscI GG'CGCG_CC
Cuts at: 0 599 2220
Size: 599 1621
AvaI C'yCGr_G
Cuts at: 0 351 488 1108 1387 1533 2220
Size: 351 137 620 279 146 687
Fragments arranged by size:
687 620 351 279 146 137
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1148 1181 2220
Size: 1148 33 1039
Fragments arranged by size:
1148 1039 33
BamHI G'GATC_C
Cuts at: 0 1030 2220
Size: 1030 1190
BanI G'GyrC_C
Cuts at: 0 1156 1700 1706 1826 1930 2220
Size: 1156 544 6 120 104 290
Fragments arranged by size:
1156 544 290 120 104 6
BanII G_rGCy'C
Cuts at: 0 103 284 352 428 558 1240 1453 2008
Size: 103 181 68 76 130 682 213 555
Cuts at: 2008 2207 2220
Size: 199 13
Fragments arranged by size:
682 555 213 199 181 130 103 76
68 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1733 1982 2220
Size: 1733 249 238
Fragments arranged by size:
1733 249 238
BbvCI CC'TCA_GC
Cuts at: 0 385 1490 1717 2220
Size: 385 1105 227 503
Fragments arranged by size:
1105 503 385 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1089 1123 2220
Size: 1089 34 1097
Fragments arranged by size:
1097 1089 34
BglI GCCn_nnn'nGGC
Cuts at: 0 209 366 577 1966 2220
Size: 209 157 211 1389 254
Fragments arranged by size:
1389 254 211 209 157
BlpI GC'TnA_GC
Cuts at: 0 433 2220
Size: 433 1787
Bme1580I G_kGCm'C
Cuts at: 0 103 352 421 428 1453 1711 1806 1933
Size: 103 249 69 7 1025 258 95 127
Cuts at: 1933 2220
Size: 287
Fragments arranged by size:
1025 287 258 249 127 103 95 69
7
BmgBI CAC'GTC
Cuts at: 0 1781 2220
Size: 1781 439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 313 608 751 1311 2019 2191 2220
Size: 217 96 295 143 560 708 172 29
Fragments arranged by size:
708 560 295 217 172 143 96 29
Bpu10I CC'TnA_GC
Cuts at: 0 268 385 991 1490 1717 2220
Size: 268 117 606 499 227 503
Fragments arranged by size:
606 503 499 268 227 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 888 1420 1995 2220
Size: 888 532 575 225
Fragments arranged by size:
888 575 532 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 668 1196 1762 2220
Size: 668 528 566 458
Fragments arranged by size:
668 566 528 458
BsaBI GATnn'nnATC
Cuts at: 0 1646 2220
Size: 1646 574
BsaHI Gr'CG_yC
Cuts at: 0 242 1631 2220
Size: 242 1389 589
Fragments arranged by size:
1389 589 242
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1309 1339 2220
Size: 1309 30 881
Fragments arranged by size:
1309 881 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 955 1388 1600 1603 1621 2016 2220
Size: 511 444 433 212 3 18 395 204
Fragments arranged by size:
511 444 433 395 212 204 18 3
BseYI C'CCAG_C
Cuts at: 0 43 47 344 543 789 1067 1171 1364
Size: 43 4 297 199 246 278 104 193
Cuts at: 1364 1853 2032 2133 2199 2220
Size: 489 179 101 66 21
Fragments arranged by size:
489 297 278 246 199 193 179 104
101 66 43 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 519 2220
Size: 355 164 1701
Fragments arranged by size:
1701 355 164
BsiHKAI G_wGCw'C
Cuts at: 0 274 284 421 2220
Size: 274 10 137 1799
Fragments arranged by size:
1799 274 137 10
Bsp1286I G_dGCh'C
Cuts at: 0 103 274 284 352 421 428 558 1240
Size: 103 171 10 68 69 7 130 682
Cuts at: 1240 1453 1711 1806 1933 2008 2207 2220
Size: 213 258 95 127 75 199 13
Fragments arranged by size:
682 258 213 199 171 130 127 103
95 75 69 68 13 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 294 686 770 1303 1670 2220
Size: 263 31 392 84 533 367 550
Fragments arranged by size:
550 533 392 367 263 84 31
BsrBI CCG'CTC
Cuts at: 0 78 406 1139 2220
Size: 78 328 733 1081
Fragments arranged by size:
1081 733 328 78
BsrFI r'CCGG_y
Cuts at: 0 360 1703 2220
Size: 360 1343 517
Fragments arranged by size:
1343 517 360
BssHII G'CGCG_C
Cuts at: 0 261 599 1016 2220
Size: 261 338 417 1204
Fragments arranged by size:
1204 417 338 261
BssSI C'ACGA_G
Cuts at: 0 1464 2220
Size: 1464 756
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1431 2094 2220
Size: 1431 663 126
Fragments arranged by size:
1431 663 126
BstYI r'GATC_y
Cuts at: 0 627 1030 1832 2220
Size: 627 403 802 388
Fragments arranged by size:
802 627 403 388
Bsu36I CC'TnA_GG
Cuts at: 0 703 2220
Size: 703 1517
BtgI C'CryG_G
Cuts at: 0 158 1772 1892 1984 2093 2220
Size: 158 1614 120 92 109 127
Fragments arranged by size:
1614 158 127 120 109 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 552 1283 2220
Size: 315 237 731 937
Fragments arranged by size:
937 731 315 237
BtsI GCAGTG_nn'
Cuts at: 0 120 226 2220
Size: 120 106 1994
Fragments arranged by size:
1994 120 106
ClaI AT'CG_AT
Cuts at: 0 913 2220
Size: 913 1307
DraIII CAC_nnn'GTG
Cuts at: 0 1013 2163 2220
Size: 1013 1150 57
Fragments arranged by size:
1150 1013 57
EaeI y'GGCC_r
Cuts at: 0 285 401 1258 1433 1497 1958 2220
Size: 285 116 857 175 64 461 262
Fragments arranged by size:
857 461 285 262 175 116 64
EarI CTCTTCn'nnn_
Cuts at: 0 85 2220
Size: 85 2135
EcoICRI GAG'CTC
Cuts at: 0 282 2220
Size: 282 1938
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 313 608 751 1311 2019 2191 2220
Size: 217 96 295 143 560 708 172 29
Fragments arranged by size:
708 560 295 217 172 143 96 29
EcoO109I rG'GnC_Cy
Cuts at: 0 72 99 100 348 843 906 1224 1361
Size: 72 27 1 248 495 63 318 137
Cuts at: 1361 1450 2220
Size: 89 770
Fragments arranged by size:
770 495 318 248 137 89 72 63
27 1
EcoRV GAT'ATC
Cuts at: 0 1410 2220
Size: 1410 810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2052 2220
Size: 2052 168
FspI TGC'GCA
Cuts at: 0 232 2220
Size: 232 1988
HaeII r_GCGC'y
Cuts at: 0 204 457 481 1789 2148 2220
Size: 204 253 24 1308 359 72
Fragments arranged by size:
1308 359 253 204 72 24
KpnI G_GTAC'C
Cuts at: 0 1160 1830 2220
Size: 1160 670 390
Fragments arranged by size:
1160 670 390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 2220
Size: 1115 1105
MscI TGG'CCA
Cuts at: 0 1435 2220
Size: 1435 785
MslI CAynn'nnrTG
Cuts at: 0 1268 1799 2095 2220
Size: 1268 531 296 125
Fragments arranged by size:
1268 531 296 125
MspA1I CmG'CkG
Cuts at: 0 18 47 160 300 399 471 635 771
Size: 18 29 113 140 99 72 164 136
Cuts at: 771 1024 1037 1071 1417 1721 2072 2199 2220
Size: 253 13 34 346 304 351 127 21
Fragments arranged by size:
351 346 304 253 164 140 136 127
113 99 72 34 29 21 18 13
NaeI GCC'GGC
Cuts at: 0 362 2220
Size: 362 1858
NgoMIV G'CCGG_C
Cuts at: 0 360 2220
Size: 360 1860
NspI r_CATG'y
Cuts at: 0 1384 2111 2220
Size: 1384 727 109
Fragments arranged by size:
1384 727 109
PasI CC'CwG_GG
Cuts at: 0 104 558 1340 2220
Size: 104 454 782 880
Fragments arranged by size:
880 782 454 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 1872 2133 2220
Size: 1872 261 87
Fragments arranged by size:
1872 261 87
PfoI T'CCnGG_A
Cuts at: 0 623 2220
Size: 623 1597
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 2220
Size: 565 32 1623
Fragments arranged by size:
1623 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 72 843 906 1361 2220
Size: 72 771 63 455 859
Fragments arranged by size:
859 771 455 72 63
PspOMI G'GGCC_C
Cuts at: 0 99 348 424 1449 2220
Size: 99 249 76 1025 771
Fragments arranged by size:
1025 771 249 99 76
PstI C_TGCA'G
Cuts at: 0 305 485 767 1667 1688 1757 2119 2144
Size: 305 180 282 900 21 69 362 25
Cuts at: 2144 2220
Size: 76
Fragments arranged by size:
900 362 305 282 180 76 69 25
21
PvuII CAG'CTG
Cuts at: 0 47 300 399 771 1024 1071 1417 2220
Size: 47 253 99 372 253 47 346 803
Fragments arranged by size:
803 372 346 253 253 99 47 47
SacI G_AGCT'C
Cuts at: 0 284 2220
Size: 284 1936
SacII CC_GC'GG
Cuts at: 0 161 2220
Size: 161 2059
SapI GCTCTTCn'nnn_
Cuts at: 0 85 2220
Size: 85 2135
SexAI A'CCwGG_T
Cuts at: 0 2159 2220
Size: 2159 61
SfcI C'TryA_G
Cuts at: 0 301 481 763 838 1307 1578 1663 1684
Size: 301 180 282 75 469 271 85 21
Cuts at: 1684 1753 2115 2140 2220
Size: 69 362 25 80
Fragments arranged by size:
469 362 301 282 271 180 85 80
75 69 25 21
SmaI CCC'GGG
Cuts at: 0 353 490 2220
Size: 353 137 1730
Fragments arranged by size:
1730 353 137
SmlI C'TyrA_G
Cuts at: 0 867 1399 1974 2220
Size: 867 532 575 246
Fragments arranged by size:
867 575 532 246
StyI C'CwwG_G
Cuts at: 0 883 1220 2220
Size: 883 337 1000
Fragments arranged by size:
1000 883 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1136 2220
Size: 1136 1084
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 2220
Size: 1309 32 879
Fragments arranged by size:
1309 879 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1443 2220
Size: 1443 777
XmaI C'CCGG_G
Cuts at: 0 351 488 2220
Size: 351 137 1732
Fragments arranged by size:
1732 351 137
ZraI GAC'GTC
Cuts at: 0 1632 2220
Size: 1632 588
Enzymes that do cut:
AarI AatII Acc65I AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AscI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI FspI
HaeII KpnI MmeI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SapI SexAI SfcI SmaI SmlI
StyI TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AcuI AflII AgeI AhdI AloI ApoI
AseI AsiSI AvrII BbeI BciVI BclI BfrBI BglII
BmrI BmtI BplI BsaAI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstBI BstEII
BstZ17I CspCI DraI DrdI EagI EciI EcoNI EcoRI
FseI FspAI Hin4I HincII HindIII HpaI KasI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmnI