Restriction map for
pF1KB5741
(Linear) (Six-base) MAPSORT of: KIBB5741.seq Check: 6801 from: 1 to: 2397
KIBB5741.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1897 2397
Size: 1897 500
AatII G_ACGT'C
Cuts at: 0 317 2397
Size: 317 2080
Acc65I G'GTAC_C
Cuts at: 0 587 2397
Size: 587 1810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 2397
Size: 1267 1130
AleI CACnn'nnGTG
Cuts at: 0 2002 2014 2086 2397
Size: 2002 12 72 311
Fragments arranged by size:
2002 311 72 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 789 1340 2397
Size: 789 551 1057
Fragments arranged by size:
1057 789 551
AlwNI CAG_nnn'CTG
Cuts at: 0 1533 2397
Size: 1533 864
ApaI G_GGCC'C
Cuts at: 0 12 2292 2397
Size: 12 2280 105
Fragments arranged by size:
2280 105 12
ApoI r'AATT_y
Cuts at: 0 1038 2397
Size: 1038 1359
AseI AT'TA_AT
Cuts at: 0 1115 2397
Size: 1115 1282
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 571 604 1113 1146 2397
Size: 571 33 509 33 1251
Fragments arranged by size:
1251 571 509 33 33
BanI G'GyrC_C
Cuts at: 0 15 481 579 587 1483 2397
Size: 15 466 98 8 896 914
Fragments arranged by size:
914 896 466 98 15 8
BanII G_rGCy'C
Cuts at: 0 12 44 244 301 2096 2292 2397
Size: 12 32 200 57 1795 196 105
Fragments arranged by size:
1795 200 196 105 57 32 12
BbeI G_GCGC'C
Cuts at: 0 19 2397
Size: 19 2378
BbvCI CC'TCA_GC
Cuts at: 0 280 2397
Size: 280 2117
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 471 505 907 941 2397
Size: 471 34 402 34 1456
Fragments arranged by size:
1456 471 402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1141 1224 2397
Size: 1141 83 1173
Fragments arranged by size:
1173 1141 83
BclI T'GATC_A
Cuts at: 0 1994 2397
Size: 1994 403
BfrBI ATG'CAT
Cuts at: 0 1120 1900 2397
Size: 1120 780 497
Fragments arranged by size:
1120 780 497
BglII A'GATC_T
Cuts at: 0 1625 1799 2397
Size: 1625 174 598
Fragments arranged by size:
1625 598 174
Bme1580I G_kGCm'C
Cuts at: 0 12 484 842 1486 1820 1885 2292 2397
Size: 12 472 358 644 334 65 407 105
Fragments arranged by size:
644 472 407 358 334 105 65 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 2093 2397
Size: 2093 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1378 2397
Size: 745 633 1019
Fragments arranged by size:
1019 745 633
Bpu10I CC'TnA_GC
Cuts at: 0 280 1689 2397
Size: 280 1409 708
Fragments arranged by size:
1409 708 280
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 2397
Size: 76 2321
BsaAI yAC'GTr
Cuts at: 0 1385 2397
Size: 1385 1012
BsaBI GATnn'nnATC
Cuts at: 0 1178 2397
Size: 1178 1219
BsaHI Gr'CG_yC
Cuts at: 0 16 314 2397
Size: 16 298 2083
Fragments arranged by size:
2083 298 16
BsaWI w'CCGG_w
Cuts at: 0 470 524 1133 2249 2397
Size: 470 54 609 1116 148
Fragments arranged by size:
1116 609 470 148 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 860 890 1306 1336 1582 1612 1642 1672
Size: 860 30 416 30 246 30 30 30
Cuts at: 1672 2122 2152 2397
Size: 450 30 245
Fragments arranged by size:
860 450 416 246 245 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 282 511 2397
Size: 282 229 1886
Fragments arranged by size:
1886 282 229
BseYI C'CCAG_C
Cuts at: 0 243 308 1300 2397
Size: 243 65 992 1097
Fragments arranged by size:
1097 992 243 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1365 1485 1584 2397
Size: 898 467 120 99 813
Fragments arranged by size:
898 813 467 120 99
BsiEI CG_ry'CG
Cuts at: 0 1336 2250 2397
Size: 1336 914 147
Fragments arranged by size:
1336 914 147
BsiHKAI G_wGCw'C
Cuts at: 0 148 1600 2096 2397
Size: 148 1452 496 301
Fragments arranged by size:
1452 496 301 148
BsmI GAATG_Cn'
Cuts at: 0 1723 2397
Size: 1723 674
Bsp1286I G_dGCh'C
Cuts at: 0 12 44 148 244 301 484 842 1486
Size: 12 32 104 96 57 183 358 644
Cuts at: 1486 1600 1820 1885 2096 2292 2397
Size: 114 220 65 211 196 105
Fragments arranged by size:
644 358 220 211 196 183 114 105
104 96 65 57 32 12
BspEI T'CCGG_A
Cuts at: 0 470 2397
Size: 470 1927
BspHI T'CATG_A
Cuts at: 0 2072 2397
Size: 2072 325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 58 1207 1723 1897 1975 2397
Size: 58 1149 516 174 78 422
Fragments arranged by size:
1149 516 422 174 78 58
BsrBI CCG'CTC
Cuts at: 0 1436 2397
Size: 1436 961
BsrFI r'CCGG_y
Cuts at: 0 1335 2397
Size: 1335 1062
BsrGI T'GTAC_A
Cuts at: 0 1031 2397
Size: 1031 1366
BssHII G'CGCG_C
Cuts at: 0 47 2397
Size: 47 2350
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1490 1595 2397
Size: 1490 105 802
Fragments arranged by size:
1490 802 105
BstEII G'GTnAC_C
Cuts at: 0 1368 2397
Size: 1368 1029
BstXI CCAn_nnnn'nTGG
Cuts at: 0 939 2172 2397
Size: 939 1233 225
Fragments arranged by size:
1233 939 225
BstYI r'GATC_y
Cuts at: 0 1173 1625 1799 2397
Size: 1173 452 174 598
Fragments arranged by size:
1173 598 452 174
Bsu36I CC'TnA_GG
Cuts at: 0 265 737 1169 2125 2397
Size: 265 472 432 956 272
Fragments arranged by size:
956 472 432 272 265
BtgI C'CryG_G
Cuts at: 0 4 169 1695 2397
Size: 4 165 1526 702
Fragments arranged by size:
1526 702 165 4
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 831 2397
Size: 831 1566
BtsI GCAGTG_nn'
Cuts at: 0 805 923 1013 1707 1840 2397
Size: 805 118 90 694 133 557
Fragments arranged by size:
805 694 557 133 118 90
DraIII CAC_nnn'GTG
Cuts at: 0 1490 2346 2397
Size: 1490 856 51
Fragments arranged by size:
1490 856 51
EaeI y'GGCC_r
Cuts at: 0 891 1333 1794 2397
Size: 891 442 461 603
Fragments arranged by size:
891 603 461 442
EagI C'GGCC_G
Cuts at: 0 1333 2397
Size: 1333 1064
EarI CTCTTCn'nnn_
Cuts at: 0 1189 2397
Size: 1189 1208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 640 2397
Size: 640 1757
EcoICRI GAG'CTC
Cuts at: 0 2094 2397
Size: 2094 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1267 1378 2397
Size: 745 522 111 1019
Fragments arranged by size:
1019 745 522 111
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 1665 2397
Size: 907 758 732
Fragments arranged by size:
907 758 732
EcoO109I rG'GnC_Cy
Cuts at: 0 9 1439 1856 2288 2397
Size: 9 1430 417 432 109
Fragments arranged by size:
1430 432 417 109 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 474 2397
Size: 390 84 1923
Fragments arranged by size:
1923 390 84
HaeII r_GCGC'y
Cuts at: 0 19 1421 2397
Size: 19 1402 976
Fragments arranged by size:
1402 976 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 930 934 962 966 1072 1104 1198 1230
Size: 930 4 28 4 106 32 94 32
Cuts at: 1230 1249 1281 1642 1674 1907 1939 2397
Size: 19 32 361 32 233 32 458
Fragments arranged by size:
930 458 361 233 106 94 32 32
32 32 32 28 19 4 4
KasI G'GCGC_C
Cuts at: 0 15 2397
Size: 15 2382
KpnI G_GTAC'C
Cuts at: 0 591 2397
Size: 591 1806
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 956 2135 2397
Size: 956 1179 262
Fragments arranged by size:
1179 956 262
MscI TGG'CCA
Cuts at: 0 893 1796 2397
Size: 893 903 601
Fragments arranged by size:
903 893 601
MslI CAynn'nnrTG
Cuts at: 0 322 2002 2014 2086 2397
Size: 322 1680 12 72 311
Fragments arranged by size:
1680 322 311 72 12
MspA1I CmG'CkG
Cuts at: 0 6 21 1568 1586 2397
Size: 6 15 1547 18 811
Fragments arranged by size:
1547 811 18 15 6
NarI GG'CG_CC
Cuts at: 0 16 2397
Size: 16 2381
NcoI C'CATG_G
Cuts at: 0 1695 2397
Size: 1695 702
NdeI CA'TA_TG
Cuts at: 0 79 2397
Size: 79 2318
NsiI A_TGCA'T
Cuts at: 0 1122 1902 2397
Size: 1122 780 495
Fragments arranged by size:
1122 780 495
NspI r_CATG'y
Cuts at: 0 1321 1781 1904 2397
Size: 1321 460 123 493
Fragments arranged by size:
1321 493 460 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 889 2140 2397
Size: 889 1251 257
Fragments arranged by size:
1251 889 257
PfoI T'CCnGG_A
Cuts at: 0 718 1346 2397
Size: 718 628 1051
Fragments arranged by size:
1051 718 628
PmlI CAC'GTG
Cuts at: 0 1385 2397
Size: 1385 1012
PpuMI rG'GwC_Cy
Cuts at: 0 1439 2397
Size: 1439 958
PshAI GACnn'nnGTC
Cuts at: 0 1444 2397
Size: 1444 953
PspOMI G'GGCC_C
Cuts at: 0 8 2288 2397
Size: 8 2280 109
Fragments arranged by size:
2280 109 8
PstI C_TGCA'G
Cuts at: 0 306 1396 1585 1807 2397
Size: 306 1090 189 222 590
Fragments arranged by size:
1090 590 306 222 189
PvuII CAG'CTG
Cuts at: 0 1586 2397
Size: 1586 811
SacI G_AGCT'C
Cuts at: 0 2096 2397
Size: 2096 301
SacII CC_GC'GG
Cuts at: 0 7 2397
Size: 7 2390
ScaI AGT'ACT
Cuts at: 0 758 2397
Size: 758 1639
SexAI A'CCwGG_T
Cuts at: 0 1486 2397
Size: 1486 911
SfcI C'TryA_G
Cuts at: 0 302 382 1392 1581 1803 2397
Size: 302 80 1010 189 222 594
Fragments arranged by size:
1010 594 302 222 189 80
SfoI GGC'GCC
Cuts at: 0 17 2397
Size: 17 2380
SpeI A'CTAG_T
Cuts at: 0 85 2397
Size: 85 2312
SphI G_CATG'C
Cuts at: 0 1904 2397
Size: 1904 493
SspI AAT'ATT
Cuts at: 0 1098 2397
Size: 1098 1299
StuI AGG'CCT
Cuts at: 0 872 2397
Size: 872 1525
StyI C'CwwG_G
Cuts at: 0 1695 2060 2397
Size: 1695 365 337
Fragments arranged by size:
1695 365 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 143 1992 2397
Size: 143 1849 405
Fragments arranged by size:
1849 405 143
TatI w'GTAC_w
Cuts at: 0 419 756 920 1031 2397
Size: 419 337 164 111 1366
Fragments arranged by size:
1366 419 337 164 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 860 892 1052 1084 1708 1740 2122 2154
Size: 860 32 160 32 624 32 382 32
Cuts at: 2154 2397
Size: 243
Fragments arranged by size:
860 624 382 243 160 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2068 2397
Size: 2068 329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 137 2343 2397
Size: 137 2206 54
Fragments arranged by size:
2206 137 54
ZraI GAC'GTC
Cuts at: 0 315 2397
Size: 315 2082
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AlfI AlwNI ApaI
ApoI AseI BaeI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NarI NcoI
NdeI NsiI NspI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SacII ScaI SexAI SfcI
SfoI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AloI
ApaLI AscI AsiSI AvaI AvrII BamHI BbsI BglI
BlpI BmgBI BmtI BplI BpuEI BsiWI BsmBI BsrDI
BssSI BstBI BstZ17I ClaI CspCI DraI DrdI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI MfeI
MluI NaeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI
SmlI SnaBI SrfI SwaI XbaI XhoI XmaI XmnI