Restriction map for
pF1KB5746
(Linear) (Six-base) MAPSORT of: KIBB5746.seq Check: 2096 from: 1 to: 2238
KIBB5746.orf
With 182 enzymes: SgfI *
January 20, 2017 09:25 ..
AccI GT'mk_AC
Cuts at: 0 1271 1398 2238
Size: 1271 127 840
Fragments arranged by size:
1271 840 127
AleI CACnn'nnGTG
Cuts at: 0 592 2238
Size: 592 1646
AlwNI CAG_nnn'CTG
Cuts at: 0 745 2133 2238
Size: 745 1388 105
Fragments arranged by size:
1388 745 105
ApaI G_GGCC'C
Cuts at: 0 637 2238
Size: 637 1601
ApaLI G'TGCA_C
Cuts at: 0 643 2238
Size: 643 1595
AvaI C'yCGr_G
Cuts at: 0 80 1874 2238
Size: 80 1794 364
Fragments arranged by size:
1794 364 80
BanI G'GyrC_C
Cuts at: 0 95 2238
Size: 95 2143
BanII G_rGCy'C
Cuts at: 0 441 637 956 1151 2238
Size: 441 196 319 195 1087
Fragments arranged by size:
1087 441 319 196 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 1446 2238
Size: 1446 792
BciVI GTATCCnnnnn_n'
Cuts at: 0 1073 1690 1881 2238
Size: 1073 617 191 357
Fragments arranged by size:
1073 617 357 191
BglI GCCn_nnn'nGGC
Cuts at: 0 859 1108 2238
Size: 859 249 1130
Fragments arranged by size:
1130 859 249
BglII A'GATC_T
Cuts at: 0 1914 2238
Size: 1914 324
Bme1580I G_kGCm'C
Cuts at: 0 637 647 2238
Size: 637 10 1591
Fragments arranged by size:
1591 637 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1044 1855 2238
Size: 1044 811 383
Fragments arranged by size:
1044 811 383
Bpu10I CC'TnA_GC
Cuts at: 0 1530 2238
Size: 1530 708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 636 2238
Size: 549 87 1602
Fragments arranged by size:
1602 549 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 1819 2238
Size: 77 1742 419
Fragments arranged by size:
1742 419 77
BsaAI yAC'GTr
Cuts at: 0 648 2238
Size: 648 1590
BsaBI GATnn'nnATC
Cuts at: 0 226 2238
Size: 226 2012
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 1179 1988 2238
Size: 28 1151 809 250
Fragments arranged by size:
1151 809 250 28
BseYI C'CCAG_C
Cuts at: 0 368 972 2115 2238
Size: 368 604 1143 123
Fragments arranged by size:
1143 604 368 123
BsiHKAI G_wGCw'C
Cuts at: 0 647 771 956 1002 1141 1151 2238
Size: 647 124 185 46 139 10 1087
Fragments arranged by size:
1087 647 185 139 124 46 10
BsmI GAATG_Cn'
Cuts at: 0 865 1992 2238
Size: 865 1127 246
Fragments arranged by size:
1127 865 246
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1693 2238
Size: 1693 545
Bsp1286I G_dGCh'C
Cuts at: 0 441 637 647 771 956 1002 1141 1151
Size: 441 196 10 124 185 46 139 10
Cuts at: 1151 2238
Size: 1087
Fragments arranged by size:
1087 441 196 185 139 124 46 10
10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1384 2238
Size: 1384 854
BsrDI GCAATG_nn'
Cuts at: 0 1019 1286 1514 1751 2002 2238
Size: 1019 267 228 237 251 236
Fragments arranged by size:
1019 267 251 237 236 228
BsrFI r'CCGG_y
Cuts at: 0 1254 2238
Size: 1254 984
BssSI C'ACGA_G
Cuts at: 0 955 2238
Size: 955 1283
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 1053 2238
Size: 591 462 1185
Fragments arranged by size:
1185 591 462
BstYI r'GATC_y
Cuts at: 0 1346 1914 2214 2238
Size: 1346 568 300 24
Fragments arranged by size:
1346 568 300 24
BtgI C'CryG_G
Cuts at: 0 937 2084 2238
Size: 937 1147 154
Fragments arranged by size:
1147 937 154
BtsI GCAGTG_nn'
Cuts at: 0 608 678 1097 2134 2238
Size: 608 70 419 1037 104
Fragments arranged by size:
1037 608 419 104 70
DraIII CAC_nnn'GTG
Cuts at: 0 150 2238
Size: 150 2088
EarI CTCTTCn'nnn_
Cuts at: 0 326 794 1715 1905 2236 2238
Size: 326 468 921 190 331 2
Fragments arranged by size:
921 468 331 326 190 2
EcoICRI GAG'CTC
Cuts at: 0 954 1149 2238
Size: 954 195 1089
Fragments arranged by size:
1089 954 195
EcoNI CCTnn'n_nnAGG
Cuts at: 0 29 799 1795 2238
Size: 29 770 996 443
Fragments arranged by size:
996 770 443 29
EcoO109I rG'GnC_Cy
Cuts at: 0 35 633 634 2238
Size: 35 598 1 1604
Fragments arranged by size:
1604 598 35 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 1542 1574 1758 1790 2238
Size: 712 32 798 32 184 32 448
Fragments arranged by size:
798 712 448 184 32 32 32
HincII GTy'rAC
Cuts at: 0 128 1399 1784 2238
Size: 128 1271 385 454
Fragments arranged by size:
1271 454 385 128
HindIII A'AGCT_T
Cuts at: 0 2032 2238
Size: 2032 206
MfeI C'AATT_G
Cuts at: 0 56 1928 2238
Size: 56 1872 310
Fragments arranged by size:
1872 310 56
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 2238
Size: 1012 1226
MslI CAynn'nnrTG
Cuts at: 0 592 1132 2238
Size: 592 540 1106
Fragments arranged by size:
1106 592 540
MspA1I CmG'CkG
Cuts at: 0 745 1290 1425 2238
Size: 745 545 135 813
Fragments arranged by size:
813 745 545 135
NcoI C'CATG_G
Cuts at: 0 937 2084 2238
Size: 937 1147 154
Fragments arranged by size:
1147 937 154
NspI r_CATG'y
Cuts at: 0 322 2238
Size: 322 1916
PflMI CCAn_nnn'nTGG
Cuts at: 0 25 2238
Size: 25 2213
PmlI CAC'GTG
Cuts at: 0 648 2238
Size: 648 1590
PpuMI rG'GwC_Cy
Cuts at: 0 35 2238
Size: 35 2203
PshAI GACnn'nnGTC
Cuts at: 0 344 2238
Size: 344 1894
PspOMI G'GGCC_C
Cuts at: 0 633 2238
Size: 633 1605
PstI C_TGCA'G
Cuts at: 0 738 1524 1623 2238
Size: 738 786 99 615
Fragments arranged by size:
786 738 615 99
PvuII CAG'CTG
Cuts at: 0 745 1290 1425 2238
Size: 745 545 135 813
Fragments arranged by size:
813 745 545 135
SacI G_AGCT'C
Cuts at: 0 956 1151 2238
Size: 956 195 1087
Fragments arranged by size:
1087 956 195
SalI G'TCGA_C
Cuts at: 0 1397 2238
Size: 1397 841
SfcI C'TryA_G
Cuts at: 0 734 755 773 1272 1388 1520 1619 2238
Size: 734 21 18 499 116 132 99 619
Fragments arranged by size:
734 619 499 132 116 99 21 18
SmaI CCC'GGG
Cuts at: 0 1876 2238
Size: 1876 362
SmlI C'TyrA_G
Cuts at: 0 80 528 615 2238
Size: 80 448 87 1623
Fragments arranged by size:
1623 448 87 80
StyI C'CwwG_G
Cuts at: 0 590 854 937 2084 2238
Size: 590 264 83 1147 154
Fragments arranged by size:
1147 590 264 154 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 54 1063 1629 2037 2097 2238
Size: 54 1009 566 408 60 141
Fragments arranged by size:
1009 566 408 141 60 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 2238
Size: 992 32 1214
Fragments arranged by size:
1214 992 32
XhoI C'TCGA_G
Cuts at: 0 80 2238
Size: 80 2158
XmaI C'CCGG_G
Cuts at: 0 1874 2238
Size: 1874 364
XmnI GAAnn'nnTTC
Cuts at: 0 1216 2238
Size: 1216 1022
Enzymes that do cut:
AccI AleI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BciVI BglI BglII Bme1580I BmrI Bpu10I BpuEI
BsaI BsaAI BsaBI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI BtgI
BtsI DraIII EarI EcoICRI EcoNI EcoO109I Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NcoI NspI PflMI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SalI
SfcI SmaI SmlI StyI TaqII TstI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BlpI BmgBI BmtI BplI BpmI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EaeI EagI EciI Eco57MI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI ZraI