Restriction map for
pF1KB5748
(Linear) (Six-base) MAPSORT of: KIBB5748.seq Check: 3533 from: 1 to: 2238
KIBB5748.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 898 2238
Size: 898 1340
AccI GT'mk_AC
Cuts at: 0 347 353 2238
Size: 347 6 1885
Fragments arranged by size:
1885 347 6
Acc65I G'GTAC_C
Cuts at: 0 809 1772 2238
Size: 809 963 466
Fragments arranged by size:
963 809 466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1127 1235 1945 2238
Size: 1017 110 108 710 293
Fragments arranged by size:
1017 710 293 110 108
AhdI GACnn_n'nnGTC
Cuts at: 0 495 2238
Size: 495 1743
AleI CACnn'nnGTG
Cuts at: 0 1597 2238
Size: 1597 641
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1831 1863 2238
Size: 1831 32 375
Fragments arranged by size:
1831 375 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1867 2238
Size: 1867 371
ApaLI G'TGCA_C
Cuts at: 0 232 511 2238
Size: 232 279 1727
Fragments arranged by size:
1727 279 232
ApoI r'AATT_y
Cuts at: 0 1435 2238
Size: 1435 803
AseI AT'TA_AT
Cuts at: 0 1115 2238
Size: 1115 1123
AvaI C'yCGr_G
Cuts at: 0 2060 2238
Size: 2060 178
BanI G'GyrC_C
Cuts at: 0 809 1772 2238
Size: 809 963 466
Fragments arranged by size:
963 809 466
BanII G_rGCy'C
Cuts at: 0 421 2238
Size: 421 1817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2090 2124 2238
Size: 2090 34 114
Fragments arranged by size:
2090 114 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 468 1813 2238
Size: 468 1345 425
Fragments arranged by size:
1345 468 425
BlpI GC'TnA_GC
Cuts at: 0 1078 1684 2238
Size: 1078 606 554
Fragments arranged by size:
1078 606 554
Bme1580I G_kGCm'C
Cuts at: 0 236 515 1533 2238
Size: 236 279 1018 705
Fragments arranged by size:
1018 705 279 236
BmgBI CAC'GTC
Cuts at: 0 618 855 2238
Size: 618 237 1383
Fragments arranged by size:
1383 618 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 1515 2238
Size: 140 1375 723
Fragments arranged by size:
1375 723 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 405 1063 1367 2238
Size: 252 153 658 304 871
Fragments arranged by size:
871 658 304 252 153
BsaI GGTCTCn'nnnn_
Cuts at: 0 482 1476 2202 2238
Size: 482 994 726 36
Fragments arranged by size:
994 726 482 36
BsaAI yAC'GTr
Cuts at: 0 351 1318 2238
Size: 351 967 920
Fragments arranged by size:
967 920 351
BsaBI GATnn'nnATC
Cuts at: 0 1824 2238
Size: 1824 414
BsaHI Gr'CG_yC
Cuts at: 0 1676 2238
Size: 1676 562
BsaWI w'CCGG_w
Cuts at: 0 150 2238
Size: 150 2088
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 753 760 783 1831 1861 1885 1915
Size: 730 23 7 23 1048 30 24 30
Cuts at: 1915 2238
Size: 323
Fragments arranged by size:
1048 730 323 30 30 24 23 23
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 761 871 2238
Size: 108 653 110 1367
Fragments arranged by size:
1367 653 110 108
BseYI C'CCAG_C
Cuts at: 0 442 1481 2096 2238
Size: 442 1039 615 142
Fragments arranged by size:
1039 615 442 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 967 2238
Size: 553 414 1271
Fragments arranged by size:
1271 553 414
BsiHKAI G_wGCw'C
Cuts at: 0 236 515 881 1685 2238
Size: 236 279 366 804 553
Fragments arranged by size:
804 553 366 279 236
BsmI GAATG_Cn'
Cuts at: 0 846 2238
Size: 846 1392
Bsp1286I G_dGCh'C
Cuts at: 0 236 421 515 881 1533 1685 2238
Size: 236 185 94 366 652 152 553
Fragments arranged by size:
652 553 366 236 185 152 94
BspEI T'CCGG_A
Cuts at: 0 150 2238
Size: 150 2088
BspHI T'CATG_A
Cuts at: 0 1967 2238
Size: 1967 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 898 1919 2238
Size: 898 1021 319
Fragments arranged by size:
1021 898 319
BsrBI CCG'CTC
Cuts at: 0 1812 2238
Size: 1812 426
BsrDI GCAATG_nn'
Cuts at: 0 1089 2238
Size: 1089 1149
BssHII G'CGCG_C
Cuts at: 0 1780 2238
Size: 1780 458
BssSI C'ACGA_G
Cuts at: 0 1010 1501 2238
Size: 1010 491 737
Fragments arranged by size:
1010 737 491
BstAPI GCAn_nnn'nTGC
Cuts at: 0 290 2238
Size: 290 1948
BstEII G'GTnAC_C
Cuts at: 0 1458 2238
Size: 1458 780
BstYI r'GATC_y
Cuts at: 0 523 2238
Size: 523 1715
BstZ17I GTA'TAC
Cuts at: 0 354 2238
Size: 354 1884
BtgI C'CryG_G
Cuts at: 0 1850 2238
Size: 1850 388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 655 1157 2238
Size: 655 502 1081
Fragments arranged by size:
1081 655 502
BtsI GCAGTG_nn'
Cuts at: 0 1439 1592 2238
Size: 1439 153 646
Fragments arranged by size:
1439 646 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1213 1248 1703 1738 2238
Size: 1213 35 455 35 500
Fragments arranged by size:
1213 500 455 35 35
DraI TTT'AAA
Cuts at: 0 1421 2187 2238
Size: 1421 766 51
Fragments arranged by size:
1421 766 51
DraIII CAC_nnn'GTG
Cuts at: 0 1742 2238
Size: 1742 496
EaeI y'GGCC_r
Cuts at: 0 407 633 647 1946 2238
Size: 407 226 14 1299 292
Fragments arranged by size:
1299 407 292 226 14
EarI CTCTTCn'nnn_
Cuts at: 0 799 2238
Size: 799 1439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 405 1017 1063 1127 1235 1367 1945
Size: 252 153 612 46 64 108 132 578
Cuts at: 1945 2238
Size: 293
Fragments arranged by size:
612 578 293 252 153 132 108 64
46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1693 2238
Size: 1693 545
EcoO109I rG'GnC_Cy
Cuts at: 0 1028 2238
Size: 1028 1210
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 481 513 2142 2174 2182 2214
Size: 250 32 199 32 1629 32 8 32
Cuts at: 2214 2238
Size: 24
Fragments arranged by size:
1629 250 199 32 32 32 32 24
8
HincII GTy'rAC
Cuts at: 0 1859 2238
Size: 1859 379
HindIII A'AGCT_T
Cuts at: 0 1495 2238
Size: 1495 743
KpnI G_GTAC'C
Cuts at: 0 813 1776 2238
Size: 813 963 462
Fragments arranged by size:
963 813 462
MfeI C'AATT_G
Cuts at: 0 208 1942 1950 2238
Size: 208 1734 8 288
Fragments arranged by size:
1734 288 208 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 2238
Size: 702 1536
MscI TGG'CCA
Cuts at: 0 649 1948 2238
Size: 649 1299 290
Fragments arranged by size:
1299 649 290
MslI CAynn'nnrTG
Cuts at: 0 1597 1830 1966 2238
Size: 1597 233 136 272
Fragments arranged by size:
1597 272 233 136
MspA1I CmG'CkG
Cuts at: 0 37 122 1583 2129 2148 2238
Size: 37 85 1461 546 19 90
Fragments arranged by size:
1461 546 90 85 37 19
NcoI C'CATG_G
Cuts at: 0 1850 2238
Size: 1850 388
NspI r_CATG'y
Cuts at: 0 789 2145 2238
Size: 789 1356 93
Fragments arranged by size:
1356 789 93
PfoI T'CCnGG_A
Cuts at: 0 173 1040 1344 2238
Size: 173 867 304 894
Fragments arranged by size:
894 867 304 173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1831 1863 2238
Size: 1831 32 375
Fragments arranged by size:
1831 375 32
PpuMI rG'GwC_Cy
Cuts at: 0 1028 2238
Size: 1028 1210
PstI C_TGCA'G
Cuts at: 0 1446 1512 2238
Size: 1446 66 726
Fragments arranged by size:
1446 726 66
PvuII CAG'CTG
Cuts at: 0 37 2129 2238
Size: 37 2092 109
Fragments arranged by size:
2092 109 37
ScaI AGT'ACT
Cuts at: 0 666 1525 2238
Size: 666 859 713
Fragments arranged by size:
859 713 666
SexAI A'CCwGG_T
Cuts at: 0 2192 2238
Size: 2192 46
SfcI C'TryA_G
Cuts at: 0 1442 1508 2238
Size: 1442 66 730
Fragments arranged by size:
1442 730 66
SnaBI TAC'GTA
Cuts at: 0 351 2238
Size: 351 1887
SspI AAT'ATT
Cuts at: 0 1301 1885 2238
Size: 1301 584 353
Fragments arranged by size:
1301 584 353
StyI C'CwwG_G
Cuts at: 0 199 1850 2238
Size: 199 1651 388
Fragments arranged by size:
1651 388 199
TatI w'GTAC_w
Cuts at: 0 664 782 1523 2238
Size: 664 118 741 715
Fragments arranged by size:
741 715 664 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 753 785 2238
Size: 753 32 1453
Fragments arranged by size:
1453 753 32
Tth111I GACn'n_nGTC
Cuts at: 0 2176 2194 2238
Size: 2176 18 44
Fragments arranged by size:
2176 44 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 999 2238
Size: 999 1239
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AloI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BcgI BciVI
BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI
BstAPI BstEII BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NcoI NspI PfoI PpiI PpuMI PstI PvuII ScaI
SexAI SfcI SnaBI SspI StyI TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AlfI ApaI
AscI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BclI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI
BsiEI BsiWI BsmBI BsrFI BsrGI BstBI BstXI Bsu36I
ClaI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI StuI SwaI TaqII XbaI XhoI
XmaI XmnI ZraI