Restriction map for
pF1KB5749
(Linear) (Six-base) MAPSORT of: KIBB5749.seq Check: 5695 from: 1 to: 2424
KIBB5749.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 0 656 2424
Size: 656 1768
AccI GT'mk_AC
Cuts at: 0 575 1364 2424
Size: 575 789 1060
Fragments arranged by size:
1060 789 575
Acc65I G'GTAC_C
Cuts at: 0 7 2424
Size: 7 2417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 2112 2327 2424
Size: 750 1362 215 97
Fragments arranged by size:
1362 750 215 97
AhdI GACnn_n'nnGTC
Cuts at: 0 1239 2424
Size: 1239 1185
AleI CACnn'nnGTG
Cuts at: 0 493 1004 2424
Size: 493 511 1420
Fragments arranged by size:
1420 511 493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 969 2424
Size: 969 1455
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 2424
Size: 1062 1362
ApoI r'AATT_y
Cuts at: 0 89 1228 2424
Size: 89 1139 1196
Fragments arranged by size:
1196 1139 89
AvaI C'yCGr_G
Cuts at: 0 1088 2424
Size: 1088 1336
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 32 2424
Size: 32 2392
BamHI G'GATC_C
Cuts at: 0 1467 2424
Size: 1467 957
BanI G'GyrC_C
Cuts at: 0 7 2424
Size: 7 2417
BanII G_rGCy'C
Cuts at: 0 1117 1813 2336 2424
Size: 1117 696 523 88
Fragments arranged by size:
1117 696 523 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 463 2083 2424
Size: 86 377 1620 341
Fragments arranged by size:
1620 377 341 86
BbvCI CC'TCA_GC
Cuts at: 0 361 833 2424
Size: 361 472 1591
Fragments arranged by size:
1591 472 361
BciVI GTATCCnnnnn_n'
Cuts at: 0 928 2424
Size: 928 1496
BclI T'GATC_A
Cuts at: 0 116 2424
Size: 116 2308
BglII A'GATC_T
Cuts at: 0 1487 2424
Size: 1487 937
BlpI GC'TnA_GC
Cuts at: 0 815 2424
Size: 815 1609
BmgBI CAC'GTC
Cuts at: 0 330 2424
Size: 330 2094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2355 2424
Size: 2355 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 1142 1817 1993 2424
Size: 492 650 675 176 431
Fragments arranged by size:
675 650 492 431 176
Bpu10I CC'TnA_GC
Cuts at: 0 361 833 1472 2424
Size: 361 472 639 952
Fragments arranged by size:
952 639 472 361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 2138 2424
Size: 571 1567 286
Fragments arranged by size:
1567 571 286
BsaI GGTCTCn'nnnn_
Cuts at: 0 974 1028 2182 2424
Size: 974 54 1154 242
Fragments arranged by size:
1154 974 242 54
BsaAI yAC'GTr
Cuts at: 0 297 2424
Size: 297 2127
BsaBI GATnn'nnATC
Cuts at: 0 2384 2424
Size: 2384 40
BsaHI Gr'CG_yC
Cuts at: 0 653 2424
Size: 653 1771
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 301 550 1107 2047 2155 2424
Size: 226 75 249 557 940 108 269
Fragments arranged by size:
940 557 269 249 226 108 75
BseYI C'CCAG_C
Cuts at: 0 624 2424
Size: 624 1800
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 1712 2424
Size: 1067 645 712
Fragments arranged by size:
1067 712 645
BsiHKAI G_wGCw'C
Cuts at: 0 1527 1813 2424
Size: 1527 286 611
Fragments arranged by size:
1527 611 286
BsmI GAATG_Cn'
Cuts at: 0 1332 1696 2236 2424
Size: 1332 364 540 188
Fragments arranged by size:
1332 540 364 188
BsmBI CGTCTCn'nnnn_
Cuts at: 0 336 2424
Size: 336 2088
Bsp1286I G_dGCh'C
Cuts at: 0 1117 1527 1813 2336 2424
Size: 1117 410 286 523 88
Fragments arranged by size:
1117 523 410 286 88
BspHI T'CATG_A
Cuts at: 0 1700 2424
Size: 1700 724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 1361 1922 2424
Size: 167 1194 561 502
Fragments arranged by size:
1194 561 502 167
BsrBI CCG'CTC
Cuts at: 0 644 2424
Size: 644 1780
BsrDI GCAATG_nn'
Cuts at: 0 1295 1766 2424
Size: 1295 471 658
Fragments arranged by size:
1295 658 471
BsrFI r'CCGG_y
Cuts at: 0 134 188 628 2424
Size: 134 54 440 1796
Fragments arranged by size:
1796 440 134 54
BstEII G'GTnAC_C
Cuts at: 0 694 2424
Size: 694 1730
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 906 1006 1197 2424
Size: 495 411 100 191 1227
Fragments arranged by size:
1227 495 411 191 100
BstYI r'GATC_y
Cuts at: 0 1467 1487 2351 2424
Size: 1467 20 864 73
Fragments arranged by size:
1467 864 73 20
BtgI C'CryG_G
Cuts at: 0 11 337 921 1005 1190 2424
Size: 11 326 584 84 185 1234
Fragments arranged by size:
1234 584 326 185 84 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1559 2424
Size: 1559 865
BtsI GCAGTG_nn'
Cuts at: 0 435 1079 1344 1420 2424
Size: 435 644 265 76 1004
Fragments arranged by size:
1004 644 435 265 76
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1749 1784 2424
Size: 1749 35 640
Fragments arranged by size:
1749 640 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1752 2389 2424
Size: 1752 637 35
Fragments arranged by size:
1752 637 35
EaeI y'GGCC_r
Cuts at: 0 630 896 1107 1580 1721 1757 1871 2424
Size: 630 266 211 473 141 36 114 553
Fragments arranged by size:
630 553 473 266 211 141 114 36
EarI CTCTTCn'nnn_
Cuts at: 0 172 1450 1769 2024 2056 2062 2324 2346
Size: 172 1278 319 255 32 6 262 22
Cuts at: 2346 2424
Size: 78
Fragments arranged by size:
1278 319 262 255 172 78 32 22
6
EcoICRI GAG'CTC
Cuts at: 0 1811 2424
Size: 1811 613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 750 1142 1817 1993 2112 2327 2424
Size: 492 258 392 675 176 119 215 97
Fragments arranged by size:
675 492 392 258 215 176 119 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1747 2424
Size: 1747 677
EcoO109I rG'GnC_Cy
Cuts at: 0 621 761 2424
Size: 621 140 1663
Fragments arranged by size:
1663 621 140
EcoRV GAT'ATC
Cuts at: 0 882 1575 2424
Size: 882 693 849
Fragments arranged by size:
882 849 693
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 2424
Size: 900 1524
FspI TGC'GCA
Cuts at: 0 163 1792 2279 2424
Size: 163 1629 487 145
Fragments arranged by size:
1629 487 163 145
HaeII r_GCGC'y
Cuts at: 0 1173 2424
Size: 1173 1251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1225 1257 1996 2028 2424
Size: 1225 32 739 32 396
Fragments arranged by size:
1225 739 396 32 32
KpnI G_GTAC'C
Cuts at: 0 11 2424
Size: 11 2413
MscI TGG'CCA
Cuts at: 0 898 1582 1723 1873 2424
Size: 898 684 141 150 551
Fragments arranged by size:
898 684 551 150 141
MslI CAynn'nnrTG
Cuts at: 0 493 926 932 1004 1717 2424
Size: 493 433 6 72 713 707
Fragments arranged by size:
713 707 493 433 72 6
MspA1I CmG'CkG
Cuts at: 0 231 339 365 894 1334 1351 2424
Size: 231 108 26 529 440 17 1073
Fragments arranged by size:
1073 529 440 231 108 26 17
NaeI GCC'GGC
Cuts at: 0 630 2424
Size: 630 1794
NcoI C'CATG_G
Cuts at: 0 921 1190 2424
Size: 921 269 1234
Fragments arranged by size:
1234 921 269
NgoMIV G'CCGG_C
Cuts at: 0 628 2424
Size: 628 1796
PflMI CCAn_nnn'nTGG
Cuts at: 0 2208 2424
Size: 2208 216
PfoI T'CCnGG_A
Cuts at: 0 65 602 732 2264 2424
Size: 65 537 130 1532 160
Fragments arranged by size:
1532 537 160 130 65
PpuMI rG'GwC_Cy
Cuts at: 0 761 2424
Size: 761 1663
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 274 306 2424
Size: 274 32 2118
Fragments arranged by size:
2118 274 32
PstI C_TGCA'G
Cuts at: 0 1339 1919 2424
Size: 1339 580 505
Fragments arranged by size:
1339 580 505
PvuII CAG'CTG
Cuts at: 0 365 894 1351 2424
Size: 365 529 457 1073
Fragments arranged by size:
1073 529 457 365
SacI G_AGCT'C
Cuts at: 0 1813 2424
Size: 1813 611
SacII CC_GC'GG
Cuts at: 0 340 2424
Size: 340 2084
SapI GCTCTTCn'nnn_
Cuts at: 0 1450 2324 2424
Size: 1450 874 100
Fragments arranged by size:
1450 874 100
SbfI CC_TGCA'GG
Cuts at: 0 1919 2424
Size: 1919 505
SfcI C'TryA_G
Cuts at: 0 1335 1411 1915 2424
Size: 1335 76 504 509
Fragments arranged by size:
1335 509 504 76
SmlI C'TyrA_G
Cuts at: 0 550 2153 2424
Size: 550 1603 271
Fragments arranged by size:
1603 550 271
SnaBI TAC'GTA
Cuts at: 0 297 2424
Size: 297 2127
SspI AAT'ATT
Cuts at: 0 1020 2424
Size: 1020 1404
StyI C'CwwG_G
Cuts at: 0 527 875 921 1190 1301 1556 2192 2424
Size: 527 348 46 269 111 255 636 232
Fragments arranged by size:
636 527 348 269 255 232 111 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1220 1639 2424
Size: 1220 419 785
Fragments arranged by size:
1220 785 419
TatI w'GTAC_w
Cuts at: 0 1709 2157 2424
Size: 1709 448 267
Fragments arranged by size:
1709 448 267
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1575 1607 2424
Size: 1575 32 817
Fragments arranged by size:
1575 817 32
Tth111I GACn'n_nGTC
Cuts at: 0 934 1498 2424
Size: 934 564 926
Fragments arranged by size:
934 926 564
XmnI GAAnn'nnTTC
Cuts at: 0 2236 2424
Size: 2236 188
ZraI GAC'GTC
Cuts at: 0 654 2424
Size: 654 1770
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AleI AlfI AlwNI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BciVI BclI BglII BlpI BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI FspI HaeII Hin4I KpnI MscI MslI MspA1I
NaeI NcoI NgoMIV PflMI PfoI PpuMI PsrI PstI
PvuII SacI SacII SapI SbfI SfcI SmlI SnaBI
SspI StyI TaqII TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BbeI BcgI BfrBI
BglI Bme1580I BmrI BmtI BsaWI BsaXI BsiEI BsiWI
BspEI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
ClaI DraI DraIII EagI EciI EcoRI FseI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SalI SanDI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI
XbaI XcmI XhoI XmaI