Restriction map for
pF1KB5758
(Linear) (Six-base) MAPSORT of: KIBB5758.seq Check: 6511 from: 1 to: 2217
KIBB5758.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 266 272 356 584 1756 1940 2217
Size: 266 6 84 228 1172 184 277
Fragments arranged by size:
1172 277 266 228 184 84 6
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1652 1797 2217
Size: 403 1249 145 420
Fragments arranged by size:
1249 420 403 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 2217
Size: 776 1441
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1760 1792 2217
Size: 1760 32 425
Fragments arranged by size:
1760 425 32
AlwNI CAG_nnn'CTG
Cuts at: 0 286 525 898 2217
Size: 286 239 373 1319
Fragments arranged by size:
1319 373 286 239
ApoI r'AATT_y
Cuts at: 0 589 918 981 1033 1615 2217
Size: 589 329 63 52 582 602
Fragments arranged by size:
602 589 582 329 63 52
AseI AT'TA_AT
Cuts at: 0 1202 2217
Size: 1202 1015
BanI G'GyrC_C
Cuts at: 0 192 2217
Size: 192 2025
BanII G_rGCy'C
Cuts at: 0 293 2217
Size: 293 1924
BbvCI CC'TCA_GC
Cuts at: 0 106 179 2217
Size: 106 73 2038
Fragments arranged by size:
2038 106 73
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 151 2217
Size: 117 34 2066
Fragments arranged by size:
2066 117 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2177 2217
Size: 2177 40
BclI T'GATC_A
Cuts at: 0 402 1019 1664 2217
Size: 402 617 645 553
Fragments arranged by size:
645 617 553 402
BglI GCCn_nnn'nGGC
Cuts at: 0 66 2217
Size: 66 2151
BglII A'GATC_T
Cuts at: 0 225 998 2093 2217
Size: 225 773 1095 124
Fragments arranged by size:
1095 773 225 124
BlpI GC'TnA_GC
Cuts at: 0 287 2217
Size: 287 1930
BmrI ACTGGGnnnn_n'
Cuts at: 0 1064 1490 2217
Size: 1064 426 727
Fragments arranged by size:
1064 727 426
BmtI G_CTAG'C
Cuts at: 0 208 2217
Size: 208 2009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 547 806 884 1253 2217
Size: 513 34 259 78 369 964
Fragments arranged by size:
964 513 369 259 78 34
Bpu10I CC'TnA_GC
Cuts at: 0 106 179 554 2217
Size: 106 73 375 1663
Fragments arranged by size:
1663 375 106 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 2217
Size: 906 1311
BsaI GGTCTCn'nnnn_
Cuts at: 0 882 2217
Size: 882 1335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 71 101 215 245 316 346 1169 1199
Size: 71 30 114 30 71 30 823 30
Cuts at: 1199 2149 2179 2217
Size: 950 30 38
Fragments arranged by size:
950 823 114 71 71 38 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 402 2217
Size: 196 206 1815
Fragments arranged by size:
1815 206 196
BseYI C'CCAG_C
Cuts at: 0 731 2107 2217
Size: 731 1376 110
Fragments arranged by size:
1376 731 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 2217
Size: 884 1333
BsiHKAI G_wGCw'C
Cuts at: 0 36 293 1058 2217
Size: 36 257 765 1159
Fragments arranged by size:
1159 765 257 36
Bsp1286I G_dGCh'C
Cuts at: 0 36 293 1058 2217
Size: 36 257 765 1159
Fragments arranged by size:
1159 765 257 36
BsrBI CCG'CTC
Cuts at: 0 43 1159 2217
Size: 43 1116 1058
Fragments arranged by size:
1116 1058 43
BsrFI r'CCGG_y
Cuts at: 0 98 2217
Size: 98 2119
BssSI C'ACGA_G
Cuts at: 0 1418 2217
Size: 1418 799
BstYI r'GATC_y
Cuts at: 0 225 521 765 998 1065 2093 2217
Size: 225 296 244 233 67 1028 124
Fragments arranged by size:
1028 296 244 233 225 124 67
BstZ17I GTA'TAC
Cuts at: 0 1757 2217
Size: 1757 460
BtsI GCAGTG_nn'
Cuts at: 0 215 287 2045 2217
Size: 215 72 1758 172
Fragments arranged by size:
1758 215 172 72
ClaI AT'CG_AT
Cuts at: 0 533 2217
Size: 533 1684
DraI TTT'AAA
Cuts at: 0 566 2217
Size: 566 1651
EaeI y'GGCC_r
Cuts at: 0 576 1230 2217
Size: 576 654 987
Fragments arranged by size:
987 654 576
EarI CTCTTCn'nnn_
Cuts at: 0 379 1669 2217
Size: 379 1290 548
Fragments arranged by size:
1290 548 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 85 2217
Size: 85 2132
EcoICRI GAG'CTC
Cuts at: 0 291 2217
Size: 291 1926
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 513 547 806 884 1253 1652 1797
Size: 403 110 34 259 78 369 399 145
Cuts at: 1797 2217
Size: 420
Fragments arranged by size:
420 403 399 369 259 145 110 78
34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1252 2217
Size: 1252 965
EcoRI G'AATT_C
Cuts at: 0 1033 1615 2217
Size: 1033 582 602
Fragments arranged by size:
1033 602 582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1453 2217
Size: 1453 764
HaeII r_GCGC'y
Cuts at: 0 1971 2217
Size: 1971 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 71 103 990 1022 1327 1359 1536 1568
Size: 71 32 887 32 305 32 177 32
Cuts at: 1568 1933 1965 2053 2078 2085 2110 2217
Size: 365 32 88 25 7 25 107
Fragments arranged by size:
887 365 305 177 107 88 71 32
32 32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 0 1652 2217
Size: 1652 565
HindIII A'AGCT_T
Cuts at: 0 407 2217
Size: 407 1810
HpaI GTT'AAC
Cuts at: 0 1652 2217
Size: 1652 565
MfeI C'AATT_G
Cuts at: 0 1508 1622 2217
Size: 1508 114 595
Fragments arranged by size:
1508 595 114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1239 1342 1356 2217
Size: 1239 103 14 861
Fragments arranged by size:
1239 861 103 14
MscI TGG'CCA
Cuts at: 0 578 2217
Size: 578 1639
MslI CAynn'nnrTG
Cuts at: 0 1842 2217
Size: 1842 375
MspA1I CmG'CkG
Cuts at: 0 1148 2217
Size: 1148 1069
NheI G'CTAG_C
Cuts at: 0 204 2217
Size: 204 2013
NspI r_CATG'y
Cuts at: 0 876 2217
Size: 876 1341
PflMI CCAn_nnn'nTGG
Cuts at: 0 1101 2217
Size: 1101 1116
PfoI T'CCnGG_A
Cuts at: 0 151 524 2217
Size: 151 373 1693
Fragments arranged by size:
1693 373 151
SacI G_AGCT'C
Cuts at: 0 293 2217
Size: 293 1924
SfcI C'TryA_G
Cuts at: 0 1826 2217
Size: 1826 391
SmlI C'TyrA_G
Cuts at: 0 885 2217
Size: 885 1332
SspI AAT'ATT
Cuts at: 0 480 2217
Size: 480 1737
StyI C'CwwG_G
Cuts at: 0 945 2217
Size: 945 1272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 374 2217
Size: 374 1843
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 215 247 2217
Size: 215 32 1970
Fragments arranged by size:
1970 215 32
XmnI GAAnn'nnTTC
Cuts at: 0 632 1678 1709 2217
Size: 632 1046 31 508
Fragments arranged by size:
1046 632 508 31
Enzymes that do cut:
AccI AcuI AlfI AloI AlwNI ApoI AseI BanI
BanII BbvCI BcgI BciVI BclI BglI BglII BlpI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstYI
BstZ17I BtsI ClaI DraI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NheI NspI
PflMI PfoI SacI SfcI SmlI SspI StyI TaqII
TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BfrBI Bme1580I BmgBI BplI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI Bsu36I BtgI BtgZI CspCI DraIII DrdI
EagI EcoO109I EcoRV FseI FspI FspAI KasI KpnI
MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI ZraI