Restriction map for
pF1KB5763
(Linear) (Six-base) MAPSORT of: KIBB5763.seq Check: 690 from: 1 to: 2448
KIBB5763.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 2448
Size: 228 2220
AccI GT'mk_AC
Cuts at: 0 398 715 2448
Size: 398 317 1733
Fragments arranged by size:
1733 398 317
Acc65I G'GTAC_C
Cuts at: 0 677 835 2448
Size: 677 158 1613
Fragments arranged by size:
1613 677 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 328 529 568 2448
Size: 34 294 201 39 1880
Fragments arranged by size:
1880 294 201 39 34
AfeI AGC'GCT
Cuts at: 0 999 2448
Size: 999 1449
AflIII A'CryG_T
Cuts at: 0 471 2448
Size: 471 1977
AhdI GACnn_n'nnGTC
Cuts at: 0 735 2448
Size: 735 1713
AleI CACnn'nnGTG
Cuts at: 0 1753 2448
Size: 1753 695
AlwNI CAG_nnn'CTG
Cuts at: 0 1015 1018 1265 2448
Size: 1015 3 247 1183
Fragments arranged by size:
1183 1015 247 3
ApaI G_GGCC'C
Cuts at: 0 59 1651 1943 2448
Size: 59 1592 292 505
Fragments arranged by size:
1592 505 292 59
ApaLI G'TGCA_C
Cuts at: 0 621 2448
Size: 621 1827
ApoI r'AATT_y
Cuts at: 0 385 2245 2448
Size: 385 1860 203
Fragments arranged by size:
1860 385 203
AvaI C'yCGr_G
Cuts at: 0 52 688 1118 2448
Size: 52 636 430 1330
Fragments arranged by size:
1330 636 430 52
BanI G'GyrC_C
Cuts at: 0 677 773 835 943 1405 2448
Size: 677 96 62 108 462 1043
Fragments arranged by size:
1043 677 462 108 96 62
BanII G_rGCy'C
Cuts at: 0 50 59 587 695 995 1272 1651 1867
Size: 50 9 528 108 300 277 379 216
Cuts at: 1867 1943 2097 2448
Size: 76 154 351
Fragments arranged by size:
528 379 351 300 277 216 154 108
76 50 9
BbeI G_GCGC'C
Cuts at: 0 777 2448
Size: 777 1671
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 2096 2448
Size: 29 2067 352
Fragments arranged by size:
2067 352 29
BglI GCCn_nnn'nGGC
Cuts at: 0 54 230 1144 2448
Size: 54 176 914 1304
Fragments arranged by size:
1304 914 176 54
Bme1580I G_kGCm'C
Cuts at: 0 59 377 625 923 1080 1651 1943 2448
Size: 59 318 248 298 157 571 292 505
Fragments arranged by size:
571 505 318 298 292 248 157 59
BmgBI CAC'GTC
Cuts at: 0 150 2448
Size: 150 2298
BmrI ACTGGGnnnn_n'
Cuts at: 0 1253 1268 1789 1992 2448
Size: 1253 15 521 203 456
Fragments arranged by size:
1253 521 456 203 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 54 2448
Size: 54 2394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 2016 2260 2422 2448
Size: 1516 500 244 162 26
Fragments arranged by size:
1516 500 244 162 26
Bpu10I CC'TnA_GC
Cuts at: 0 527 1505 2448
Size: 527 978 943
Fragments arranged by size:
978 943 527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 571 1435 2448
Size: 301 270 864 1013
Fragments arranged by size:
1013 864 301 270
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 2448
Size: 169 2279
BsaAI yAC'GTr
Cuts at: 0 402 474 2448
Size: 402 72 1974
Fragments arranged by size:
1974 402 72
BsaHI Gr'CG_yC
Cuts at: 0 774 2084 2448
Size: 774 1310 364
Fragments arranged by size:
1310 774 364
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 919 949 1797 1827 1917 1947 2448
Size: 919 30 848 30 90 30 501
Fragments arranged by size:
919 848 501 90 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 1476 1567 1805 2448
Size: 438 1038 91 238 643
Fragments arranged by size:
1038 643 438 238 91
BseYI C'CCAG_C
Cuts at: 0 831 969 994 1483 1633 1740 1770 1832
Size: 831 138 25 489 150 107 30 62
Cuts at: 1832 1886 2141 2448
Size: 54 255 307
Fragments arranged by size:
831 489 307 255 150 138 107 62
54 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1316 1898 2448
Size: 415 901 582 550
Fragments arranged by size:
901 582 550 415
BsiEI CG_ry'CG
Cuts at: 0 951 2448
Size: 951 1497
BsiHKAI G_wGCw'C
Cuts at: 0 587 625 695 1682 2448
Size: 587 38 70 987 766
Fragments arranged by size:
987 766 587 70 38
Bsp1286I G_dGCh'C
Cuts at: 0 50 59 377 587 625 695 923 995
Size: 50 9 318 210 38 70 228 72
Cuts at: 995 1080 1272 1651 1682 1867 1943 2097 2448
Size: 85 192 379 31 185 76 154 351
Fragments arranged by size:
379 351 318 228 210 192 185 154
85 76 72 70 50 38 31 9
BspHI T'CATG_A
Cuts at: 0 650 821 2448
Size: 650 171 1627
Fragments arranged by size:
1627 650 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 437 2435 2448
Size: 228 209 1998 13
Fragments arranged by size:
1998 228 209 13
BsrBI CCG'CTC
Cuts at: 0 128 1548 1590 1611 2448
Size: 128 1420 42 21 837
Fragments arranged by size:
1420 837 128 42 21
BsrFI r'CCGG_y
Cuts at: 0 229 1466 1518 1758 2448
Size: 229 1237 52 240 690
Fragments arranged by size:
1237 690 240 229 52
BsrGI T'GTAC_A
Cuts at: 0 801 2448
Size: 801 1647
BssSI C'ACGA_G
Cuts at: 0 611 2448
Size: 611 1837
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1018 2448
Size: 1018 1430
BstEII G'GTnAC_C
Cuts at: 0 276 2448
Size: 276 2172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 564 1840 1927 1989 2448
Size: 108 456 1276 87 62 459
Fragments arranged by size:
1276 459 456 108 87 62
BstYI r'GATC_y
Cuts at: 0 254 311 1193 2393 2448
Size: 254 57 882 1200 55
Fragments arranged by size:
1200 882 254 57 55
BstZ17I GTA'TAC
Cuts at: 0 716 2448
Size: 716 1732
Bsu36I CC'TnA_GG
Cuts at: 0 298 361 2448
Size: 298 63 2087
Fragments arranged by size:
2087 298 63
BtgI C'CryG_G
Cuts at: 0 1289 2352 2448
Size: 1289 1063 96
Fragments arranged by size:
1289 1063 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 327 2448
Size: 327 2121
BtsI GCAGTG_nn'
Cuts at: 0 964 2214 2448
Size: 964 1250 234
Fragments arranged by size:
1250 964 234
DraI TTT'AAA
Cuts at: 0 327 2448
Size: 327 2121
EaeI y'GGCC_r
Cuts at: 0 2 98 231 242 665 1040 2295 2448
Size: 2 96 133 11 423 375 1255 153
Fragments arranged by size:
1255 423 375 153 133 96 11 2
EarI CTCTTCn'nnn_
Cuts at: 0 787 2448
Size: 787 1661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 201 1174 1578 2448
Size: 201 973 404 870
Fragments arranged by size:
973 870 404 201
EcoICRI GAG'CTC
Cuts at: 0 585 693 2448
Size: 585 108 1755
Fragments arranged by size:
1755 585 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 328 529 568 1516 2016 2260 2422
Size: 34 294 201 39 948 500 244 162
Cuts at: 2422 2448
Size: 26
Fragments arranged by size:
948 500 294 244 201 162 39 34
26
EcoO109I rG'GnC_Cy
Cuts at: 0 191 504 957 1637 1647 1648 2169 2442
Size: 191 313 453 680 10 1 521 273
Cuts at: 2442 2448
Size: 6
Fragments arranged by size:
680 521 453 313 273 191 10 6
1
EcoRI G'AATT_C
Cuts at: 0 385 2245 2448
Size: 385 1860 203
Fragments arranged by size:
1860 385 203
HaeII r_GCGC'y
Cuts at: 0 777 1001 1539 1777 2448
Size: 777 224 538 238 671
Fragments arranged by size:
777 671 538 238 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 721 753 1664 1696 2296 2328 2362 2394
Size: 721 32 911 32 600 32 34 32
Cuts at: 2394 2448
Size: 54
Fragments arranged by size:
911 721 600 54 34 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2130 2448
Size: 2130 318
KasI G'GCGC_C
Cuts at: 0 773 2448
Size: 773 1675
KpnI G_GTAC'C
Cuts at: 0 681 839 2448
Size: 681 158 1609
Fragments arranged by size:
1609 681 158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1685 1824 2448
Size: 581 1104 139 624
Fragments arranged by size:
1104 624 581 139
MscI TGG'CCA
Cuts at: 0 244 667 1042 2297 2448
Size: 244 423 375 1255 151
Fragments arranged by size:
1255 423 375 244 151
MslI CAynn'nnrTG
Cuts at: 0 823 826 1753 2272 2448
Size: 823 3 927 519 176
Fragments arranged by size:
927 823 519 176 3
MspA1I CmG'CkG
Cuts at: 0 85 634 1015 1542 2448
Size: 85 549 381 527 906
Fragments arranged by size:
906 549 527 381 85
NaeI GCC'GGC
Cuts at: 0 1468 1760 2448
Size: 1468 292 688
Fragments arranged by size:
1468 688 292
NarI GG'CG_CC
Cuts at: 0 774 2448
Size: 774 1674
NcoI C'CATG_G
Cuts at: 0 1289 2352 2448
Size: 1289 1063 96
Fragments arranged by size:
1289 1063 96
NgoMIV G'CCGG_C
Cuts at: 0 1466 1758 2448
Size: 1466 292 690
Fragments arranged by size:
1466 690 292
NspI r_CATG'y
Cuts at: 0 519 981 2448
Size: 519 462 1467
Fragments arranged by size:
1467 519 462
PasI CC'CwG_GG
Cuts at: 0 923 1265 2017 2448
Size: 923 342 752 431
Fragments arranged by size:
923 752 431 342
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 674 1265 1906 2017 2293 2448
Size: 240 434 591 641 111 276 155
Fragments arranged by size:
641 591 434 276 240 155 111
PfoI T'CCnGG_A
Cuts at: 0 407 2438 2448
Size: 407 2031 10
Fragments arranged by size:
2031 407 10
PmlI CAC'GTG
Cuts at: 0 474 2448
Size: 474 1974
PpuMI rG'GwC_Cy
Cuts at: 0 2169 2442 2448
Size: 2169 273 6
Fragments arranged by size:
2169 273 6
PspOMI G'GGCC_C
Cuts at: 0 55 1647 1939 2448
Size: 55 1592 292 509
Fragments arranged by size:
1592 509 292 55
PstI C_TGCA'G
Cuts at: 0 1361 2387 2448
Size: 1361 1026 61
Fragments arranged by size:
1361 1026 61
PvuII CAG'CTG
Cuts at: 0 1015 2448
Size: 1015 1433
SacI G_AGCT'C
Cuts at: 0 587 695 2448
Size: 587 108 1753
Fragments arranged by size:
1753 587 108
SapI GCTCTTCn'nnn_
Cuts at: 0 787 2448
Size: 787 1661
ScaI AGT'ACT
Cuts at: 0 646 2448
Size: 646 1802
SfcI C'TryA_G
Cuts at: 0 92 811 1213 1357 1826 2383 2448
Size: 92 719 402 144 469 557 65
Fragments arranged by size:
719 557 469 402 144 92 65
SfoI GGC'GCC
Cuts at: 0 775 2448
Size: 775 1673
SmaI CCC'GGG
Cuts at: 0 54 2448
Size: 54 2394
SmlI C'TyrA_G
Cuts at: 0 316 586 1450 2448
Size: 316 270 864 998
Fragments arranged by size:
998 864 316 270
SpeI A'CTAG_T
Cuts at: 0 1674 2448
Size: 1674 774
SphI G_CATG'C
Cuts at: 0 981 2448
Size: 981 1467
StyI C'CwwG_G
Cuts at: 0 1289 1583 1664 2352 2448
Size: 1289 294 81 688 96
Fragments arranged by size:
1289 688 294 96 81
TatI w'GTAC_w
Cuts at: 0 512 644 801 2448
Size: 512 132 157 1647
Fragments arranged by size:
1647 512 157 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1917 1949 2448
Size: 1917 32 499
Fragments arranged by size:
1917 499 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1491 1662 1986 2448
Size: 1491 171 324 462
Fragments arranged by size:
1491 462 324 171
XmaI C'CCGG_G
Cuts at: 0 52 2448
Size: 52 2396
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SapI ScaI SfcI SfoI SmaI SmlI SpeI
SphI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AlfI AloI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmtI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BsrDI BssHII BstBI ClaI CspCI DraIII
DrdI EagI EcoNI EcoRV FalI FseI FspI FspAI
HindIII HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SgfI SgrAI SnaBI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI