Restriction map for
pF1KB5764
(Linear) (Six-base) MAPSORT of: KIBB5764.seq Check: 1931 from: 1 to: 2268
KIBB5764.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 0 2083 2268
Size: 2083 185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1334 1349 1864 2268
Size: 187 1147 15 515 404
Fragments arranged by size:
1147 515 404 187 15
AleI CACnn'nnGTG
Cuts at: 0 1987 2268
Size: 1987 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1541 1573 2268
Size: 1541 32 695
Fragments arranged by size:
1541 695 32
AlwNI CAG_nnn'CTG
Cuts at: 0 604 1197 1309 2268
Size: 604 593 112 959
Fragments arranged by size:
959 604 593 112
ApaLI G'TGCA_C
Cuts at: 0 919 2268
Size: 919 1349
ApoI r'AATT_y
Cuts at: 0 524 2268
Size: 524 1744
AseI AT'TA_AT
Cuts at: 0 1538 2268
Size: 1538 730
AvaI C'yCGr_G
Cuts at: 0 1163 2268
Size: 1163 1105
BanI G'GyrC_C
Cuts at: 0 193 2268
Size: 193 2075
BanII G_rGCy'C
Cuts at: 0 1009 2268
Size: 1009 1259
BbsI GAAGACnn'nnnn_
Cuts at: 0 1097 2268
Size: 1097 1171
BciVI GTATCCnnnnn_n'
Cuts at: 0 1341 2268
Size: 1341 927
BfrBI ATG'CAT
Cuts at: 0 1183 2268
Size: 1183 1085
BglII A'GATC_T
Cuts at: 0 213 2268
Size: 213 2055
Bme1580I G_kGCm'C
Cuts at: 0 923 1943 2268
Size: 923 1020 325
Fragments arranged by size:
1020 923 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 988 1002 1510 2268
Size: 988 14 508 758
Fragments arranged by size:
988 758 508 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 2268
Size: 834 1434
BsaBI GATnn'nnATC
Cuts at: 0 178 2268
Size: 178 2090
BsaWI w'CCGG_w
Cuts at: 0 2057 2268
Size: 2057 211
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 584 614 2268
Size: 584 30 1654
Fragments arranged by size:
1654 584 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 498 970 1300 2092 2268
Size: 498 472 330 792 176
Fragments arranged by size:
792 498 472 330 176
BseYI C'CCAG_C
Cuts at: 0 1302 2268
Size: 1302 966
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 997 2268
Size: 934 63 1271
Fragments arranged by size:
1271 934 63
BsiHKAI G_wGCw'C
Cuts at: 0 923 2268
Size: 923 1345
BsmI GAATG_Cn'
Cuts at: 0 2041 2268
Size: 2041 227
Bsp1286I G_dGCh'C
Cuts at: 0 923 1009 1943 2268
Size: 923 86 934 325
Fragments arranged by size:
934 923 325 86
BspEI T'CCGG_A
Cuts at: 0 2057 2268
Size: 2057 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 533 2243 2268
Size: 533 1710 25
Fragments arranged by size:
1710 533 25
BsrBI CCG'CTC
Cuts at: 0 972 1743 2268
Size: 972 771 525
Fragments arranged by size:
972 771 525
BssSI C'ACGA_G
Cuts at: 0 480 952 2268
Size: 480 472 1316
Fragments arranged by size:
1316 480 472
BstXI CCAn_nnnn'nTGG
Cuts at: 0 190 2132 2268
Size: 190 1942 136
Fragments arranged by size:
1942 190 136
BstYI r'GATC_y
Cuts at: 0 179 213 2268
Size: 179 34 2055
Fragments arranged by size:
2055 179 34
BtgI C'CryG_G
Cuts at: 0 635 2268
Size: 635 1633
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 39 2268
Size: 39 2229
DraI TTT'AAA
Cuts at: 0 497 2268
Size: 497 1771
EaeI y'GGCC_r
Cuts at: 0 79 311 2268
Size: 79 232 1957
Fragments arranged by size:
1957 232 79
EarI CTCTTCn'nnn_
Cuts at: 0 784 1375 2268
Size: 784 591 893
Fragments arranged by size:
893 784 591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 988 1002 1334 1349 1510 1864 2268
Size: 187 801 14 332 15 161 354 404
Fragments arranged by size:
801 404 354 332 187 161 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 952 2268
Size: 952 1316
EcoO109I rG'GnC_Cy
Cuts at: 0 440 459 1387 2268
Size: 440 19 928 881
Fragments arranged by size:
928 881 440 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 786 1834 2268
Size: 786 1048 434
Fragments arranged by size:
1048 786 434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 1861 1893 1946 1978 2268
Size: 112 32 1717 32 53 32 290
Fragments arranged by size:
1717 290 112 53 32 32 32
HincII GTy'rAC
Cuts at: 0 2084 2268
Size: 2084 184
HindIII A'AGCT_T
Cuts at: 0 491 1174 1660 2268
Size: 491 683 486 608
Fragments arranged by size:
683 608 491 486
MscI TGG'CCA
Cuts at: 0 313 2268
Size: 313 1955
MslI CAynn'nnrTG
Cuts at: 0 1987 2268
Size: 1987 281
MspA1I CmG'CkG
Cuts at: 0 78 358 2268
Size: 78 280 1910
Fragments arranged by size:
1910 280 78
NsiI A_TGCA'T
Cuts at: 0 1185 2268
Size: 1185 1083
PflMI CCAn_nnn'nTGG
Cuts at: 0 427 795 906 1146 1783 2268
Size: 427 368 111 240 637 485
Fragments arranged by size:
637 485 427 368 240 111
PfoI T'CCnGG_A
Cuts at: 0 965 1018 1526 1562 2131 2268
Size: 965 53 508 36 569 137
Fragments arranged by size:
965 569 508 137 53 36
PpuMI rG'GwC_Cy
Cuts at: 0 440 459 1387 2268
Size: 440 19 928 881
Fragments arranged by size:
928 881 440 19
PstI C_TGCA'G
Cuts at: 0 257 1193 1476 2225 2268
Size: 257 936 283 749 43
Fragments arranged by size:
936 749 283 257 43
PvuII CAG'CTG
Cuts at: 0 358 2268
Size: 358 1910
SalI G'TCGA_C
Cuts at: 0 2082 2268
Size: 2082 186
SanDI GG'GwC_CC
Cuts at: 0 440 2268
Size: 440 1828
SfcI C'TryA_G
Cuts at: 0 253 1189 1472 2221 2268
Size: 253 936 283 749 47
Fragments arranged by size:
936 749 283 253 47
SmaI CCC'GGG
Cuts at: 0 1165 2268
Size: 1165 1103
SmlI C'TyrA_G
Cuts at: 0 813 2268
Size: 813 1455
SpeI A'CTAG_T
Cuts at: 0 244 1510 2268
Size: 244 1266 758
Fragments arranged by size:
1266 758 244
SspI AAT'ATT
Cuts at: 0 645 2268
Size: 645 1623
StuI AGG'CCT
Cuts at: 0 164 2268
Size: 164 2104
StyI C'CwwG_G
Cuts at: 0 1616 2268
Size: 1616 652
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 881 1048 2268
Size: 881 167 1220
Fragments arranged by size:
1220 881 167
TatI w'GTAC_w
Cuts at: 0 552 1025 2066 2268
Size: 552 473 1041 202
Fragments arranged by size:
1041 552 473 202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 854 886 2268
Size: 854 32 1382
Fragments arranged by size:
1382 854 32
Tth111I GACn'n_nGTC
Cuts at: 0 1101 2268
Size: 1101 1167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1784 2268
Size: 1784 484
XmaI C'CCGG_G
Cuts at: 0 1163 2268
Size: 1163 1105
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI ApaLI ApoI AseI
AvaI BanI BanII BbsI BciVI BfrBI BglII Bme1580I
BpmI BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BssSI BstXI
BstYI BtgI BtgZI DraI EaeI EarI Eco57MI EcoNI
EcoO109I FalI Hin4I HincII HindIII MscI MslI MspA1I
NsiI PflMI PfoI PpuMI PstI PvuII SalI SanDI
SfcI SmaI SmlI SpeI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaHI BsiEI BsiWI
BsmBI BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI SwaI XbaI XhoI XmnI ZraI