Restriction map for
pF1KB5771
(Linear) (Six-base) MAPSORT of: KIBB5771.seq Check: 6700 from: 1 to: 2199
KIBB5771.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 427 1194 2199
Size: 427 767 1005
Fragments arranged by size:
1005 767 427
AccI GT'mk_AC
Cuts at: 0 748 920 2199
Size: 748 172 1279
Fragments arranged by size:
1279 748 172
Acc65I G'GTAC_C
Cuts at: 0 937 2199
Size: 937 1262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1891 2199
Size: 122 1769 308
Fragments arranged by size:
1769 308 122
AflII C'TTAA_G
Cuts at: 0 714 2199
Size: 714 1485
AhdI GACnn_n'nnGTC
Cuts at: 0 1533 2199
Size: 1533 666
AleI CACnn'nnGTG
Cuts at: 0 1772 2199
Size: 1772 427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1015 2199
Size: 1015 1184
AlwNI CAG_nnn'CTG
Cuts at: 0 1021 1904 2199
Size: 1021 883 295
Fragments arranged by size:
1021 883 295
ApaLI G'TGCA_C
Cuts at: 0 973 2199
Size: 973 1226
ApoI r'AATT_y
Cuts at: 0 319 2199
Size: 319 1880
AvaI C'yCGr_G
Cuts at: 0 1154 1556 1738 2137 2199
Size: 1154 402 182 399 62
Fragments arranged by size:
1154 402 399 182 62
BanI G'GyrC_C
Cuts at: 0 393 937 1734 2199
Size: 393 544 797 465
Fragments arranged by size:
797 544 465 393
BanII G_rGCy'C
Cuts at: 0 2089 2147 2199
Size: 2089 58 52
Fragments arranged by size:
2089 58 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 673 1139 1994 2109 2199
Size: 673 466 855 115 90
Fragments arranged by size:
855 673 466 115 90
BglI GCCn_nnn'nGGC
Cuts at: 0 1074 1470 2199
Size: 1074 396 729
Fragments arranged by size:
1074 729 396
BglII A'GATC_T
Cuts at: 0 659 1808 2199
Size: 659 1149 391
Fragments arranged by size:
1149 659 391
Bme1580I G_kGCm'C
Cuts at: 0 398 977 1955 2199
Size: 398 579 978 244
Fragments arranged by size:
978 579 398 244
BmrI ACTGGGnnnn_n'
Cuts at: 0 1892 2199
Size: 1892 307
BmtI G_CTAG'C
Cuts at: 0 1105 2199
Size: 1105 1094
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1440 1533 2199
Size: 1013 427 93 666
Fragments arranged by size:
1013 666 427 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 695 877 1014 1068 2199
Size: 390 305 182 137 54 1131
Fragments arranged by size:
1131 390 305 182 137 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 1116 1592 1956 2199
Size: 1116 476 364 243
Fragments arranged by size:
1116 476 364 243
BsaAI yAC'GTr
Cuts at: 0 752 2093 2199
Size: 752 1341 106
Fragments arranged by size:
1341 752 106
BsaBI GATnn'nnATC
Cuts at: 0 865 2199
Size: 865 1334
BsaWI w'CCGG_w
Cuts at: 0 1754 2199
Size: 1754 445
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 421 451 544 574 736 766 2044 2074
Size: 421 30 93 30 162 30 1278 30
Cuts at: 2074 2199
Size: 125
Fragments arranged by size:
1278 421 162 125 93 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 93 123 157 1071 1509 1522 2088
Size: 10 83 30 34 914 438 13 566
Cuts at: 2088 2155 2199
Size: 67 44
Fragments arranged by size:
914 566 438 83 67 44 34 30
13 10
BseYI C'CCAG_C
Cuts at: 0 1142 1374 1492 2065 2163 2168 2199
Size: 1142 232 118 573 98 5 31
Fragments arranged by size:
1142 573 232 118 98 31 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1368 1890 2199
Size: 39 1329 522 309
Fragments arranged by size:
1329 522 309 39
BsiEI CG_ry'CG
Cuts at: 0 1713 2199
Size: 1713 486
BsiHKAI G_wGCw'C
Cuts at: 0 977 1799 2089 2098 2199
Size: 977 822 290 9 101
Fragments arranged by size:
977 822 290 101 9
Bsp1286I G_dGCh'C
Cuts at: 0 398 977 1799 1955 2089 2098 2147 2199
Size: 398 579 822 156 134 9 49 52
Fragments arranged by size:
822 579 398 156 134 52 49 9
BspHI T'CATG_A
Cuts at: 0 2124 2199
Size: 2124 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 653 1194 1379 1445 1457 2199
Size: 427 226 541 185 66 12 742
Fragments arranged by size:
742 541 427 226 185 66 12
BsrBI CCG'CTC
Cuts at: 0 480 2199
Size: 480 1719
BsrFI r'CCGG_y
Cuts at: 0 237 491 828 2199
Size: 237 254 337 1371
Fragments arranged by size:
1371 337 254 237
BsrGI T'GTAC_A
Cuts at: 0 569 1919 2199
Size: 569 1350 280
Fragments arranged by size:
1350 569 280
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 1950 2199
Size: 234 1716 249
Fragments arranged by size:
1716 249 234
BstEII G'GTnAC_C
Cuts at: 0 1879 2199
Size: 1879 320
BstXI CCAn_nnnn'nTGG
Cuts at: 0 340 1530 2049 2199
Size: 340 1190 519 150
Fragments arranged by size:
1190 519 340 150
BstYI r'GATC_y
Cuts at: 0 659 1757 1808 2199
Size: 659 1098 51 391
Fragments arranged by size:
1098 659 391 51
BstZ17I GTA'TAC
Cuts at: 0 749 2199
Size: 749 1450
Bsu36I CC'TnA_GG
Cuts at: 0 2100 2199
Size: 2100 99
BtsI GCAGTG_nn'
Cuts at: 0 239 292 839 1955 2199
Size: 239 53 547 1116 244
Fragments arranged by size:
1116 547 244 239 53
DrdI GACnn_nn'nnGTC
Cuts at: 0 1165 2199
Size: 1165 1034
EaeI y'GGCC_r
Cuts at: 0 983 1066 1710 2199
Size: 983 83 644 489
Fragments arranged by size:
983 644 489 83
EagI C'GGCC_G
Cuts at: 0 1710 2199
Size: 1710 489
EarI CTCTTCn'nnn_
Cuts at: 0 28 141 815 925 940 2199
Size: 28 113 674 110 15 1259
Fragments arranged by size:
1259 674 113 110 28 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2198 2199
Size: 2198 1
EcoICRI GAG'CTC
Cuts at: 0 2087 2199
Size: 2087 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 390 695 877 1014 1068 1891 2199
Size: 122 268 305 182 137 54 823 308
Fragments arranged by size:
823 308 305 268 182 137 122 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 2199
Size: 1186 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 278 881 1379 1463 1527 2199
Size: 278 603 498 84 64 672
Fragments arranged by size:
672 603 498 278 84 64
EcoRI G'AATT_C
Cuts at: 0 319 2199
Size: 319 1880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 969 2199
Size: 969 1230
FspI TGC'GCA
Cuts at: 0 1003 2199
Size: 1003 1196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 118 147 150 1303 1335 1519 1551
Size: 115 3 29 3 1153 32 184 32
Cuts at: 1551 1768 1800 1832 2044 2076 2199
Size: 217 32 32 212 32 123
Fragments arranged by size:
1153 217 212 184 123 115 32 32
32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 0 800 2199
Size: 800 1399
KpnI G_GTAC'C
Cuts at: 0 941 2199
Size: 941 1258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1265 2042 2199
Size: 1265 777 157
Fragments arranged by size:
1265 777 157
MscI TGG'CCA
Cuts at: 0 985 2199
Size: 985 1214
MslI CAynn'nnrTG
Cuts at: 0 379 613 1772 1927 2199
Size: 379 234 1159 155 272
Fragments arranged by size:
1159 379 272 234 155
MspA1I CmG'CkG
Cuts at: 0 465 1980 2133 2199
Size: 465 1515 153 66
Fragments arranged by size:
1515 465 153 66
NaeI GCC'GGC
Cuts at: 0 239 830 2199
Size: 239 591 1369
Fragments arranged by size:
1369 591 239
NgoMIV G'CCGG_C
Cuts at: 0 237 828 2199
Size: 237 591 1371
Fragments arranged by size:
1371 591 237
NheI G'CTAG_C
Cuts at: 0 1101 2199
Size: 1101 1098
NspI r_CATG'y
Cuts at: 0 232 1948 2199
Size: 232 1716 251
Fragments arranged by size:
1716 251 232
PasI CC'CwG_GG
Cuts at: 0 537 1182 1468 2199
Size: 537 645 286 731
Fragments arranged by size:
731 645 537 286
PpuMI rG'GwC_Cy
Cuts at: 0 278 1527 2199
Size: 278 1249 672
Fragments arranged by size:
1249 672 278
PstI C_TGCA'G
Cuts at: 0 1454 2199
Size: 1454 745
PvuII CAG'CTG
Cuts at: 0 465 1980 2199
Size: 465 1515 219
Fragments arranged by size:
1515 465 219
SacI G_AGCT'C
Cuts at: 0 2089 2199
Size: 2089 110
SanDI GG'GwC_CC
Cuts at: 0 278 2199
Size: 278 1921
SbfI CC_TGCA'GG
Cuts at: 0 1454 2199
Size: 1454 745
ScaI AGT'ACT
Cuts at: 0 2059 2199
Size: 2059 140
SexAI A'CCwGG_T
Cuts at: 0 1529 1547 2199
Size: 1529 18 652
Fragments arranged by size:
1529 652 18
SfcI C'TryA_G
Cuts at: 0 1450 2199
Size: 1450 749
SmlI C'TyrA_G
Cuts at: 0 714 2199
Size: 714 1485
SphI G_CATG'C
Cuts at: 0 232 1948 2199
Size: 232 1716 251
Fragments arranged by size:
1716 251 232
StyI C'CwwG_G
Cuts at: 0 1427 1523 2199
Size: 1427 96 676
Fragments arranged by size:
1427 676 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 900 1780 2177 2199
Size: 900 880 397 22
Fragments arranged by size:
900 880 397 22
TatI w'GTAC_w
Cuts at: 0 569 1919 2057 2199
Size: 569 1350 138 142
Fragments arranged by size:
1350 569 142 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 736 768 2199
Size: 544 32 160 32 1431
Fragments arranged by size:
1431 544 160 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1617 2176 2199
Size: 528 1089 559 23
Fragments arranged by size:
1089 559 528 23
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AhdI AleI AlfI
AlwNI ApaLI ApoI AvaI BanI BanII BbsI BglI
BglII Bme1580I BmrI BmtI BplI BpmI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI Hin4I HincII KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NheI NspI PasI PpuMI PstI PvuII
SacI SanDI SbfI ScaI SexAI SfcI SmlI SphI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AloI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BlpI BmgBI Bpu10I BpuEI BsaHI
BsiWI BsmI BsmBI BspEI BsrDI BssHII BssSI BstBI
BtgI BtgZI ClaI CspCI DraI DraIII EcoRV FseI
FspAI HaeII HindIII HpaI KasI MfeI MluI NarI
NcoI NdeI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI