(Linear) (Six-base) MAPSORT of: KIBB5771.seq Check: 6700 from: 1 to: 2199 KIBB5771.orf With 182 enzymes: SgfI * March 2, 2010 14:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 427 1194 2199 Size: 427 767 1005 Fragments arranged by size: 1005 767 427 AccI GT'mk_AC Cuts at: 0 748 920 2199 Size: 748 172 1279 Fragments arranged by size: 1279 748 172 Acc65I G'GTAC_C Cuts at: 0 937 2199 Size: 937 1262 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 1891 2199 Size: 122 1769 308 Fragments arranged by size: 1769 308 122 AflII C'TTAA_G Cuts at: 0 714 2199 Size: 714 1485 AhdI GACnn_n'nnGTC Cuts at: 0 1533 2199 Size: 1533 666 AleI CACnn'nnGTG Cuts at: 0 1772 2199 Size: 1772 427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1015 2199 Size: 1015 1184 AlwNI CAG_nnn'CTG Cuts at: 0 1021 1904 2199 Size: 1021 883 295 Fragments arranged by size: 1021 883 295 ApaLI G'TGCA_C Cuts at: 0 973 2199 Size: 973 1226 ApoI r'AATT_y Cuts at: 0 319 2199 Size: 319 1880 AvaI C'yCGr_G Cuts at: 0 1154 1556 1738 2137 2199 Size: 1154 402 182 399 62 Fragments arranged by size: 1154 402 399 182 62 BanI G'GyrC_C Cuts at: 0 393 937 1734 2199 Size: 393 544 797 465 Fragments arranged by size: 797 544 465 393 BanII G_rGCy'C Cuts at: 0 2089 2147 2199 Size: 2089 58 52 Fragments arranged by size: 2089 58 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 673 1139 1994 2109 2199 Size: 673 466 855 115 90 Fragments arranged by size: 855 673 466 115 90 BglI GCCn_nnn'nGGC Cuts at: 0 1074 1470 2199 Size: 1074 396 729 Fragments arranged by size: 1074 729 396 BglII A'GATC_T Cuts at: 0 659 1808 2199 Size: 659 1149 391 Fragments arranged by size: 1149 659 391 Bme1580I G_kGCm'C Cuts at: 0 398 977 1955 2199 Size: 398 579 978 244 Fragments arranged by size: 978 579 398 244 BmrI ACTGGGnnnn_n' Cuts at: 0 1892 2199 Size: 1892 307 BmtI G_CTAG'C Cuts at: 0 1105 2199 Size: 1105 1094 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1013 1440 1533 2199 Size: 1013 427 93 666 Fragments arranged by size: 1013 666 427 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 390 695 877 1014 1068 2199 Size: 390 305 182 137 54 1131 Fragments arranged by size: 1131 390 305 182 137 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 1116 1592 1956 2199 Size: 1116 476 364 243 Fragments arranged by size: 1116 476 364 243 BsaAI yAC'GTr Cuts at: 0 752 2093 2199 Size: 752 1341 106 Fragments arranged by size: 1341 752 106 BsaBI GATnn'nnATC Cuts at: 0 865 2199 Size: 865 1334 BsaWI w'CCGG_w Cuts at: 0 1754 2199 Size: 1754 445 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 421 451 544 574 736 766 2044 2074 Size: 421 30 93 30 162 30 1278 30 Cuts at: 2074 2199 Size: 125 Fragments arranged by size: 1278 421 162 125 93 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 10 93 123 157 1071 1509 1522 2088 Size: 10 83 30 34 914 438 13 566 Cuts at: 2088 2155 2199 Size: 67 44 Fragments arranged by size: 914 566 438 83 67 44 34 30 13 10 BseYI C'CCAG_C Cuts at: 0 1142 1374 1492 2065 2163 2168 2199 Size: 1142 232 118 573 98 5 31 Fragments arranged by size: 1142 573 232 118 98 31 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 1368 1890 2199 Size: 39 1329 522 309 Fragments arranged by size: 1329 522 309 39 BsiEI CG_ry'CG Cuts at: 0 1713 2199 Size: 1713 486 BsiHKAI G_wGCw'C Cuts at: 0 977 1799 2089 2098 2199 Size: 977 822 290 9 101 Fragments arranged by size: 977 822 290 101 9 Bsp1286I G_dGCh'C Cuts at: 0 398 977 1799 1955 2089 2098 2147 2199 Size: 398 579 822 156 134 9 49 52 Fragments arranged by size: 822 579 398 156 134 52 49 9 BspHI T'CATG_A Cuts at: 0 2124 2199 Size: 2124 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 427 653 1194 1379 1445 1457 2199 Size: 427 226 541 185 66 12 742 Fragments arranged by size: 742 541 427 226 185 66 12 BsrBI CCG'CTC Cuts at: 0 480 2199 Size: 480 1719 BsrFI r'CCGG_y Cuts at: 0 237 491 828 2199 Size: 237 254 337 1371 Fragments arranged by size: 1371 337 254 237 BsrGI T'GTAC_A Cuts at: 0 569 1919 2199 Size: 569 1350 280 Fragments arranged by size: 1350 569 280 BstAPI GCAn_nnn'nTGC Cuts at: 0 234 1950 2199 Size: 234 1716 249 Fragments arranged by size: 1716 249 234 BstEII G'GTnAC_C Cuts at: 0 1879 2199 Size: 1879 320 BstXI CCAn_nnnn'nTGG Cuts at: 0 340 1530 2049 2199 Size: 340 1190 519 150 Fragments arranged by size: 1190 519 340 150 BstYI r'GATC_y Cuts at: 0 659 1757 1808 2199 Size: 659 1098 51 391 Fragments arranged by size: 1098 659 391 51 BstZ17I GTA'TAC Cuts at: 0 749 2199 Size: 749 1450 Bsu36I CC'TnA_GG Cuts at: 0 2100 2199 Size: 2100 99 BtsI GCAGTG_nn' Cuts at: 0 239 292 839 1955 2199 Size: 239 53 547 1116 244 Fragments arranged by size: 1116 547 244 239 53 DrdI GACnn_nn'nnGTC Cuts at: 0 1165 2199 Size: 1165 1034 EaeI y'GGCC_r Cuts at: 0 983 1066 1710 2199 Size: 983 83 644 489 Fragments arranged by size: 983 644 489 83 EagI C'GGCC_G Cuts at: 0 1710 2199 Size: 1710 489 EarI CTCTTCn'nnn_ Cuts at: 0 28 141 815 925 940 2199 Size: 28 113 674 110 15 1259 Fragments arranged by size: 1259 674 113 110 28 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2198 2199 Size: 2198 1 EcoICRI GAG'CTC Cuts at: 0 2087 2199 Size: 2087 112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 122 390 695 877 1014 1068 1891 2199 Size: 122 268 305 182 137 54 823 308 Fragments arranged by size: 823 308 305 268 182 137 122 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 1186 2199 Size: 1186 1013 EcoO109I rG'GnC_Cy Cuts at: 0 278 881 1379 1463 1527 2199 Size: 278 603 498 84 64 672 Fragments arranged by size: 672 603 498 278 84 64 EcoRI G'AATT_C Cuts at: 0 319 2199 Size: 319 1880 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 969 2199 Size: 969 1230 FspI TGC'GCA Cuts at: 0 1003 2199 Size: 1003 1196 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 115 118 147 150 1303 1335 1519 1551 Size: 115 3 29 3 1153 32 184 32 Cuts at: 1551 1768 1800 1832 2044 2076 2199 Size: 217 32 32 212 32 123 Fragments arranged by size: 1153 217 212 184 123 115 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 0 800 2199 Size: 800 1399 KpnI G_GTAC'C Cuts at: 0 941 2199 Size: 941 1258 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1265 2042 2199 Size: 1265 777 157 Fragments arranged by size: 1265 777 157 MscI TGG'CCA Cuts at: 0 985 2199 Size: 985 1214 MslI CAynn'nnrTG Cuts at: 0 379 613 1772 1927 2199 Size: 379 234 1159 155 272 Fragments arranged by size: 1159 379 272 234 155 MspA1I CmG'CkG Cuts at: 0 465 1980 2133 2199 Size: 465 1515 153 66 Fragments arranged by size: 1515 465 153 66 NaeI GCC'GGC Cuts at: 0 239 830 2199 Size: 239 591 1369 Fragments arranged by size: 1369 591 239 NgoMIV G'CCGG_C Cuts at: 0 237 828 2199 Size: 237 591 1371 Fragments arranged by size: 1371 591 237 NheI G'CTAG_C Cuts at: 0 1101 2199 Size: 1101 1098 NspI r_CATG'y Cuts at: 0 232 1948 2199 Size: 232 1716 251 Fragments arranged by size: 1716 251 232 PasI CC'CwG_GG Cuts at: 0 537 1182 1468 2199 Size: 537 645 286 731 Fragments arranged by size: 731 645 537 286 PpuMI rG'GwC_Cy Cuts at: 0 278 1527 2199 Size: 278 1249 672 Fragments arranged by size: 1249 672 278 PstI C_TGCA'G Cuts at: 0 1454 2199 Size: 1454 745 PvuII CAG'CTG Cuts at: 0 465 1980 2199 Size: 465 1515 219 Fragments arranged by size: 1515 465 219 SacI G_AGCT'C Cuts at: 0 2089 2199 Size: 2089 110 SanDI GG'GwC_CC Cuts at: 0 278 2199 Size: 278 1921 SbfI CC_TGCA'GG Cuts at: 0 1454 2199 Size: 1454 745 ScaI AGT'ACT Cuts at: 0 2059 2199 Size: 2059 140 SexAI A'CCwGG_T Cuts at: 0 1529 1547 2199 Size: 1529 18 652 Fragments arranged by size: 1529 652 18 SfcI C'TryA_G Cuts at: 0 1450 2199 Size: 1450 749 SmlI C'TyrA_G Cuts at: 0 714 2199 Size: 714 1485 SphI G_CATG'C Cuts at: 0 232 1948 2199 Size: 232 1716 251 Fragments arranged by size: 1716 251 232 StyI C'CwwG_G Cuts at: 0 1427 1523 2199 Size: 1427 96 676 Fragments arranged by size: 1427 676 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 900 1780 2177 2199 Size: 900 880 397 22 Fragments arranged by size: 900 880 397 22 TatI w'GTAC_w Cuts at: 0 569 1919 2057 2199 Size: 569 1350 138 142 Fragments arranged by size: 1350 569 142 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 544 576 736 768 2199 Size: 544 32 160 32 1431 Fragments arranged by size: 1431 544 160 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 528 1617 2176 2199 Size: 528 1089 559 23 Fragments arranged by size: 1089 559 528 23 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AhdI AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BglI BglII Bme1580I BmrI BmtI BplI BpmI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NheI NspI PasI PpuMI PstI PvuII SacI SanDI SbfI ScaI SexAI SfcI SmlI SphI StyI TaqII TatI TstI XcmI Enzymes that do not cut: AatII AclI AfeI AflIII AgeI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI Bpu10I BpuEI BsaHI BsiWI BsmI BsmBI BspEI BsrDI BssHII BssSI BstBI BtgI BtgZI ClaI CspCI DraI DraIII EcoRV FseI FspAI HaeII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI