Restriction map for pF1KB5771
 (Linear) (Six-base) MAPSORT of: KIBB5771.seq  Check: 6700  from: 1  to: 2199



KIBB5771.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     427    1194    2199 
   Size:        427     767    1005

  Fragments arranged by size: 

                1005     767     427
 
AccI GT'mk_AC

Cuts at:      0     748     920    2199 
   Size:        748     172    1279

  Fragments arranged by size: 

                1279     748     172
 
Acc65I G'GTAC_C

Cuts at:      0     937    2199 
   Size:        937    1262
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122    1891    2199 
   Size:        122    1769     308

  Fragments arranged by size: 

                1769     308     122
 
AflII C'TTAA_G

Cuts at:      0     714    2199 
   Size:        714    1485
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1533    2199 
   Size:       1533     666
 
AleI CACnn'nnGTG

Cuts at:      0    1772    2199 
   Size:       1772     427
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1015    2199 
   Size:       1015    1184
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1021    1904    2199 
   Size:       1021     883     295

  Fragments arranged by size: 

                1021     883     295
 
ApaLI G'TGCA_C

Cuts at:      0     973    2199 
   Size:        973    1226
 
ApoI r'AATT_y

Cuts at:      0     319    2199 
   Size:        319    1880
 
AvaI C'yCGr_G

Cuts at:      0    1154    1556    1738    2137    2199 
   Size:       1154     402     182     399      62

  Fragments arranged by size: 

                1154     402     399     182      62
 
BanI G'GyrC_C

Cuts at:      0     393     937    1734    2199 
   Size:        393     544     797     465

  Fragments arranged by size: 

                 797     544     465     393
 
BanII G_rGCy'C

Cuts at:      0    2089    2147    2199 
   Size:       2089      58      52

  Fragments arranged by size: 

                2089      58      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     673    1139    1994    2109    2199 
   Size:        673     466     855     115      90

  Fragments arranged by size: 

                 855     673     466     115      90
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1074    1470    2199 
   Size:       1074     396     729

  Fragments arranged by size: 

                1074     729     396
 
BglII A'GATC_T

Cuts at:      0     659    1808    2199 
   Size:        659    1149     391

  Fragments arranged by size: 

                1149     659     391
 
Bme1580I G_kGCm'C

Cuts at:      0     398     977    1955    2199 
   Size:        398     579     978     244

  Fragments arranged by size: 

                 978     579     398     244
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1892    2199 
   Size:       1892     307
 
BmtI G_CTAG'C

Cuts at:      0    1105    2199 
   Size:       1105    1094
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1013    1440    1533    2199 
   Size:       1013     427      93     666

  Fragments arranged by size: 

                1013     666     427      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     695     877    1014    1068    2199 
   Size:        390     305     182     137      54    1131

  Fragments arranged by size: 

                1131     390     305     182     137      54
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1116    1592    1956    2199 
   Size:       1116     476     364     243

  Fragments arranged by size: 

                1116     476     364     243
 
BsaAI yAC'GTr

Cuts at:      0     752    2093    2199 
   Size:        752    1341     106

  Fragments arranged by size: 

                1341     752     106
 
BsaBI GATnn'nnATC

Cuts at:      0     865    2199 
   Size:        865    1334
 
BsaWI w'CCGG_w

Cuts at:      0    1754    2199 
   Size:       1754     445
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     421     451     544     574     736     766    2044    2074 
   Size:        421      30      93      30     162      30    1278      30

Cuts at:   2074    2199 
   Size:        125

  Fragments arranged by size: 

                1278     421     162     125      93      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      10      93     123     157    1071    1509    1522    2088 
   Size:         10      83      30      34     914     438      13     566

Cuts at:   2088    2155    2199 
   Size:         67      44

  Fragments arranged by size: 

                 914     566     438      83      67      44      34      30
                  13      10
 
BseYI C'CCAG_C

Cuts at:      0    1142    1374    1492    2065    2163    2168    2199 
   Size:       1142     232     118     573      98       5      31

  Fragments arranged by size: 

                1142     573     232     118      98      31       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39    1368    1890    2199 
   Size:         39    1329     522     309

  Fragments arranged by size: 

                1329     522     309      39
 
BsiEI CG_ry'CG

Cuts at:      0    1713    2199 
   Size:       1713     486
 
BsiHKAI G_wGCw'C

Cuts at:      0     977    1799    2089    2098    2199 
   Size:        977     822     290       9     101

  Fragments arranged by size: 

                 977     822     290     101       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     398     977    1799    1955    2089    2098    2147    2199 
   Size:        398     579     822     156     134       9      49      52

  Fragments arranged by size: 

                 822     579     398     156     134      52      49       9
            
 
BspHI T'CATG_A

Cuts at:      0    2124    2199 
   Size:       2124      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     427     653    1194    1379    1445    1457    2199 
   Size:        427     226     541     185      66      12     742

  Fragments arranged by size: 

                 742     541     427     226     185      66      12
 
BsrBI CCG'CTC

Cuts at:      0     480    2199 
   Size:        480    1719
 
BsrFI r'CCGG_y

Cuts at:      0     237     491     828    2199 
   Size:        237     254     337    1371

  Fragments arranged by size: 

                1371     337     254     237
 
BsrGI T'GTAC_A

Cuts at:      0     569    1919    2199 
   Size:        569    1350     280

  Fragments arranged by size: 

                1350     569     280
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     234    1950    2199 
   Size:        234    1716     249

  Fragments arranged by size: 

                1716     249     234
 
BstEII G'GTnAC_C

Cuts at:      0    1879    2199 
   Size:       1879     320
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     340    1530    2049    2199 
   Size:        340    1190     519     150

  Fragments arranged by size: 

                1190     519     340     150
 
BstYI r'GATC_y

Cuts at:      0     659    1757    1808    2199 
   Size:        659    1098      51     391

  Fragments arranged by size: 

                1098     659     391      51
 
BstZ17I GTA'TAC

Cuts at:      0     749    2199 
   Size:        749    1450
 
Bsu36I CC'TnA_GG

Cuts at:      0    2100    2199 
   Size:       2100      99
 
BtsI GCAGTG_nn'

Cuts at:      0     239     292     839    1955    2199 
   Size:        239      53     547    1116     244

  Fragments arranged by size: 

                1116     547     244     239      53
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1165    2199 
   Size:       1165    1034
 
EaeI y'GGCC_r

Cuts at:      0     983    1066    1710    2199 
   Size:        983      83     644     489

  Fragments arranged by size: 

                 983     644     489      83
 
EagI C'GGCC_G

Cuts at:      0    1710    2199 
   Size:       1710     489
 
EarI CTCTTCn'nnn_

Cuts at:      0      28     141     815     925     940    2199 
   Size:         28     113     674     110      15    1259

  Fragments arranged by size: 

                1259     674     113     110      28      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2198    2199 
   Size:       2198       1
 
EcoICRI GAG'CTC

Cuts at:      0    2087    2199 
   Size:       2087     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     390     695     877    1014    1068    1891    2199 
   Size:        122     268     305     182     137      54     823     308

  Fragments arranged by size: 

                 823     308     305     268     182     137     122      54
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1186    2199 
   Size:       1186    1013
 
EcoO109I rG'GnC_Cy

Cuts at:      0     278     881    1379    1463    1527    2199 
   Size:        278     603     498      84      64     672

  Fragments arranged by size: 

                 672     603     498     278      84      64
 
EcoRI G'AATT_C

Cuts at:      0     319    2199 
   Size:        319    1880
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     969    2199 
   Size:        969    1230
 
FspI TGC'GCA

Cuts at:      0    1003    2199 
   Size:       1003    1196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     115     118     147     150    1303    1335    1519    1551 
   Size:        115       3      29       3    1153      32     184      32

Cuts at:   1551    1768    1800    1832    2044    2076    2199 
   Size:        217      32      32     212      32     123

  Fragments arranged by size: 

                1153     217     212     184     123     115      32      32
                  32      32      32      29       3       3
 
HincII GTy'rAC

Cuts at:      0     800    2199 
   Size:        800    1399
 
KpnI G_GTAC'C

Cuts at:      0     941    2199 
   Size:        941    1258
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1265    2042    2199 
   Size:       1265     777     157

  Fragments arranged by size: 

                1265     777     157
 
MscI TGG'CCA

Cuts at:      0     985    2199 
   Size:        985    1214
 
MslI CAynn'nnrTG

Cuts at:      0     379     613    1772    1927    2199 
   Size:        379     234    1159     155     272

  Fragments arranged by size: 

                1159     379     272     234     155
 
MspA1I CmG'CkG

Cuts at:      0     465    1980    2133    2199 
   Size:        465    1515     153      66

  Fragments arranged by size: 

                1515     465     153      66
 
NaeI GCC'GGC

Cuts at:      0     239     830    2199 
   Size:        239     591    1369

  Fragments arranged by size: 

                1369     591     239
 
NgoMIV G'CCGG_C

Cuts at:      0     237     828    2199 
   Size:        237     591    1371

  Fragments arranged by size: 

                1371     591     237
 
NheI G'CTAG_C

Cuts at:      0    1101    2199 
   Size:       1101    1098
 
NspI r_CATG'y

Cuts at:      0     232    1948    2199 
   Size:        232    1716     251

  Fragments arranged by size: 

                1716     251     232
 
PasI CC'CwG_GG

Cuts at:      0     537    1182    1468    2199 
   Size:        537     645     286     731

  Fragments arranged by size: 

                 731     645     537     286
 
PpuMI rG'GwC_Cy

Cuts at:      0     278    1527    2199 
   Size:        278    1249     672

  Fragments arranged by size: 

                1249     672     278
 
PstI C_TGCA'G

Cuts at:      0    1454    2199 
   Size:       1454     745
 
PvuII CAG'CTG

Cuts at:      0     465    1980    2199 
   Size:        465    1515     219

  Fragments arranged by size: 

                1515     465     219
 
SacI G_AGCT'C

Cuts at:      0    2089    2199 
   Size:       2089     110
 
SanDI GG'GwC_CC

Cuts at:      0     278    2199 
   Size:        278    1921
 
SbfI CC_TGCA'GG

Cuts at:      0    1454    2199 
   Size:       1454     745
 
ScaI AGT'ACT

Cuts at:      0    2059    2199 
   Size:       2059     140
 
SexAI A'CCwGG_T

Cuts at:      0    1529    1547    2199 
   Size:       1529      18     652

  Fragments arranged by size: 

                1529     652      18
 
SfcI C'TryA_G

Cuts at:      0    1450    2199 
   Size:       1450     749
 
SmlI C'TyrA_G

Cuts at:      0     714    2199 
   Size:        714    1485
 
SphI G_CATG'C

Cuts at:      0     232    1948    2199 
   Size:        232    1716     251

  Fragments arranged by size: 

                1716     251     232
 
StyI C'CwwG_G

Cuts at:      0    1427    1523    2199 
   Size:       1427      96     676

  Fragments arranged by size: 

                1427     676      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     900    1780    2177    2199 
   Size:        900     880     397      22

  Fragments arranged by size: 

                 900     880     397      22
 
TatI w'GTAC_w

Cuts at:      0     569    1919    2057    2199 
   Size:        569    1350     138     142

  Fragments arranged by size: 

                1350     569     142     138
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     544     576     736     768    2199 
   Size:        544      32     160      32    1431

  Fragments arranged by size: 

                1431     544     160      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     528    1617    2176    2199 
   Size:        528    1089     559      23

  Fragments arranged by size: 

                1089     559     528      23
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AflII     AhdI     AleI     AlfI
    AlwNI    ApaLI     ApoI     AvaI     BanI    BanII     BbsI     BglI
    BglII Bme1580I     BmrI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    Hin4I   HincII     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NheI     NspI     PasI    PpuMI     PstI    PvuII
     SacI    SanDI     SbfI     ScaI    SexAI     SfcI     SmlI     SphI
     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AclI     AfeI   AflIII     AgeI     AloI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI    BmgBI   Bpu10I    BpuEI    BsaHI
    BsiWI     BsmI    BsmBI    BspEI    BsrDI   BssHII    BssSI    BstBI
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII    EcoRV     FseI
    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI     NarI
     NcoI     NdeI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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