Restriction map for
pF1KB5775
(Linear) (Six-base) MAPSORT of: KIBB5775.seq Check: 5718 from: 1 to: 2439
KIBB5775.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 298 340 368 1940 2439
Size: 272 26 42 28 1572 499
Fragments arranged by size:
1572 499 272 42 28 26
AflII C'TTAA_G
Cuts at: 0 235 1741 2439
Size: 235 1506 698
Fragments arranged by size:
1506 698 235
AflIII A'CryG_T
Cuts at: 0 1441 2439
Size: 1441 998
AlwNI CAG_nnn'CTG
Cuts at: 0 30 2439
Size: 30 2409
ApoI r'AATT_y
Cuts at: 0 595 1221 1504 2426 2439
Size: 595 626 283 922 13
Fragments arranged by size:
922 626 595 283 13
AvaI C'yCGr_G
Cuts at: 0 145 2439
Size: 145 2294
BanI G'GyrC_C
Cuts at: 0 141 194 828 2439
Size: 141 53 634 1611
Fragments arranged by size:
1611 634 141 53
BanII G_rGCy'C
Cuts at: 0 131 2439
Size: 131 2308
BbeI G_GCGC'C
Cuts at: 0 145 198 2439
Size: 145 53 2241
Fragments arranged by size:
2241 145 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 2007 2143 2439
Size: 2007 136 296
Fragments arranged by size:
2007 296 136
BclI T'GATC_A
Cuts at: 0 1802 2439
Size: 1802 637
BfrBI ATG'CAT
Cuts at: 0 2118 2320 2439
Size: 2118 202 119
Fragments arranged by size:
2118 202 119
BglI GCCn_nnn'nGGC
Cuts at: 0 73 2439
Size: 73 2366
BlpI GC'TnA_GC
Cuts at: 0 121 2439
Size: 121 2318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 241 265 2297 2439
Size: 180 61 24 2032 142
Fragments arranged by size:
2032 180 142 61 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 802 2439
Size: 802 1637
BsaAI yAC'GTr
Cuts at: 0 1442 2439
Size: 1442 997
BsaBI GATnn'nnATC
Cuts at: 0 1889 2439
Size: 1889 550
BsaHI Gr'CG_yC
Cuts at: 0 142 195 662 2439
Size: 142 53 467 1777
Fragments arranged by size:
1777 467 142 53
BsaWI w'CCGG_w
Cuts at: 0 1833 2439
Size: 1833 606
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 177 2216 2439
Size: 177 2039 223
Fragments arranged by size:
2039 223 177
BseYI C'CCAG_C
Cuts at: 0 262 2439
Size: 262 2177
BsiEI CG_ry'CG
Cuts at: 0 44 77 2439
Size: 44 33 2362
Fragments arranged by size:
2362 44 33
BsiHKAI G_wGCw'C
Cuts at: 0 131 1034 1644 2439
Size: 131 903 610 795
Fragments arranged by size:
903 795 610 131
BsmI GAATG_Cn'
Cuts at: 0 1473 2439
Size: 1473 966
BsmBI CGTCTCn'nnnn_
Cuts at: 0 669 2439
Size: 669 1770
Bsp1286I G_dGCh'C
Cuts at: 0 131 1034 1644 2439
Size: 131 903 610 795
Fragments arranged by size:
903 795 610 131
BspHI T'CATG_A
Cuts at: 0 1716 1799 2439
Size: 1716 83 640
Fragments arranged by size:
1716 640 83
BsrDI GCAATG_nn'
Cuts at: 0 1962 2099 2221 2298 2439
Size: 1962 137 122 77 141
Fragments arranged by size:
1962 141 137 122 77
BsrFI r'CCGG_y
Cuts at: 0 76 2439
Size: 76 2363
BsrGI T'GTAC_A
Cuts at: 0 1290 2439
Size: 1290 1149
BstBI TT'CG_AA
Cuts at: 0 1617 2439
Size: 1617 822
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1477 2439
Size: 1477 962
BstYI r'GATC_y
Cuts at: 0 1119 1950 2439
Size: 1119 831 489
Fragments arranged by size:
1119 831 489
Bsu36I CC'TnA_GG
Cuts at: 0 2196 2439
Size: 2196 243
BtgI C'CryG_G
Cuts at: 0 50 2238 2439
Size: 50 2188 201
Fragments arranged by size:
2188 201 50
BtsI GCAGTG_nn'
Cuts at: 0 232 542 1987 2439
Size: 232 310 1445 452
Fragments arranged by size:
1445 452 310 232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 457 492 2439
Size: 457 35 1947
Fragments arranged by size:
1947 457 35
DraI TTT'AAA
Cuts at: 0 1511 2439
Size: 1511 928
EaeI y'GGCC_r
Cuts at: 0 41 74 2439
Size: 41 33 2365
Fragments arranged by size:
2365 41 33
EagI C'GGCC_G
Cuts at: 0 41 74 2439
Size: 41 33 2365
Fragments arranged by size:
2365 41 33
EarI CTCTTCn'nnn_
Cuts at: 0 1630 2439
Size: 1630 809
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 190 2439
Size: 187 3 2249
Fragments arranged by size:
2249 187 3
EcoICRI GAG'CTC
Cuts at: 0 129 2439
Size: 129 2310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 241 265 272 298 340 368 1940
Size: 180 61 24 7 26 42 28 1572
Cuts at: 1940 2297 2439
Size: 357 142
Fragments arranged by size:
1572 357 180 142 61 42 28 26
24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 2439
Size: 136 2303
EcoO109I rG'GnC_Cy
Cuts at: 0 15 27 2439
Size: 15 12 2412
Fragments arranged by size:
2412 15 12
EcoRI G'AATT_C
Cuts at: 0 595 2439
Size: 595 1844
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 2156 2160 2439
Size: 429 1727 4 279
Fragments arranged by size:
1727 429 279 4
HaeII r_GCGC'y
Cuts at: 0 145 198 2439
Size: 145 53 2241
Fragments arranged by size:
2241 145 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 649 681 2439
Size: 649 32 1758
Fragments arranged by size:
1758 649 32
HindIII A'AGCT_T
Cuts at: 0 1457 2439
Size: 1457 982
KasI G'GCGC_C
Cuts at: 0 141 194 2439
Size: 141 53 2245
Fragments arranged by size:
2245 141 53
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 732 1097 2439
Size: 732 365 1342
Fragments arranged by size:
1342 732 365
MslI CAynn'nnrTG
Cuts at: 0 1214 2439
Size: 1214 1225
MspA1I CmG'CkG
Cuts at: 0 60 108 615 1092 1783 2439
Size: 60 48 507 477 691 656
Fragments arranged by size:
691 656 507 477 60 48
NaeI GCC'GGC
Cuts at: 0 78 2439
Size: 78 2361
NarI GG'CG_CC
Cuts at: 0 142 195 2439
Size: 142 53 2244
Fragments arranged by size:
2244 142 53
NcoI C'CATG_G
Cuts at: 0 2238 2439
Size: 2238 201
NgoMIV G'CCGG_C
Cuts at: 0 76 2439
Size: 76 2363
NsiI A_TGCA'T
Cuts at: 0 2120 2322 2439
Size: 2120 202 117
Fragments arranged by size:
2120 202 117
NspI r_CATG'y
Cuts at: 0 544 2118 2439
Size: 544 1574 321
Fragments arranged by size:
1574 544 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 981 2239 2439
Size: 981 1258 200
Fragments arranged by size:
1258 981 200
PfoI T'CCnGG_A
Cuts at: 0 131 2439
Size: 131 2308
PmlI CAC'GTG
Cuts at: 0 1442 2439
Size: 1442 997
PpuMI rG'GwC_Cy
Cuts at: 0 15 2439
Size: 15 2424
PstI C_TGCA'G
Cuts at: 0 332 2190 2439
Size: 332 1858 249
Fragments arranged by size:
1858 332 249
PvuII CAG'CTG
Cuts at: 0 615 1092 1783 2439
Size: 615 477 691 656
Fragments arranged by size:
691 656 615 477
SacI G_AGCT'C
Cuts at: 0 131 2439
Size: 131 2308
SanDI GG'GwC_CC
Cuts at: 0 15 2439
Size: 15 2424
SbfI CC_TGCA'GG
Cuts at: 0 332 2439
Size: 332 2107
ScaI AGT'ACT
Cuts at: 0 2041 2439
Size: 2041 398
SfcI C'TryA_G
Cuts at: 0 22 328 1699 2060 2186 2439
Size: 22 306 1371 361 126 253
Fragments arranged by size:
1371 361 306 253 126 22
SfoI GGC'GCC
Cuts at: 0 143 196 2439
Size: 143 53 2243
Fragments arranged by size:
2243 143 53
SmlI C'TyrA_G
Cuts at: 0 235 1741 2439
Size: 235 1506 698
Fragments arranged by size:
1506 698 235
SspI AAT'ATT
Cuts at: 0 1422 1672 2439
Size: 1422 250 767
Fragments arranged by size:
1422 767 250
StyI C'CwwG_G
Cuts at: 0 852 1709 2238 2439
Size: 852 857 529 201
Fragments arranged by size:
857 852 529 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 2439
Size: 277 2162
TatI w'GTAC_w
Cuts at: 0 1290 2039 2439
Size: 1290 749 400
Fragments arranged by size:
1290 749 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 1322 1354 2439
Size: 413 32 877 32 1085
Fragments arranged by size:
1085 877 413 32 32
XbaI T'CTAG_A
Cuts at: 0 1608 1881 2439
Size: 1608 273 558
Fragments arranged by size:
1608 558 273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1392 2257 2439
Size: 1392 865 182
Fragments arranged by size:
1392 865 182
XmnI GAAnn'nnTTC
Cuts at: 0 353 1234 2439
Size: 353 881 1205
Fragments arranged by size:
1205 881 353
Enzymes that do cut:
AcuI AflII AflIII AlwNI ApoI AvaI BanI BanII
BbeI BbsI BclI BfrBI BglI BlpI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI BstBI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HindIII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PflMI PfoI
PmlI PpuMI PstI PvuII SacI SanDI SbfI ScaI
SfcI SfoI SmlI SspI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BglII Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaXI BsgI
BsiWI BspEI BspMI BsrBI BssHII BssSI BstAPI BstEII
BstZ17I BtgZI ClaI DraIII DrdI EcoRV FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MscI NdeI
NheI NotI NruI PacI PasI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI
ZraI