Restriction map for
pF1KB5787
(Linear) (Six-base) MAPSORT of: KIBB5787.seq Check: 9226 from: 1 to: 2487
KIBB5787.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 696 2487
Size: 696 1791
AccI GT'mk_AC
Cuts at: 0 1382 2487
Size: 1382 1105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 122 280 1273 1837 1849 2305 2487
Size: 75 47 158 993 564 12 456 182
Fragments arranged by size:
993 564 456 182 158 75 47 12
AflIII A'CryG_T
Cuts at: 0 2462 2487
Size: 2462 25
AhdI GACnn_n'nnGTC
Cuts at: 0 2367 2487
Size: 2367 120
AleI CACnn'nnGTG
Cuts at: 0 934 1080 1279 1489 2487
Size: 934 146 199 210 998
Fragments arranged by size:
998 934 210 199 146
AlwNI CAG_nnn'CTG
Cuts at: 0 150 381 1044 1642 1692 2487
Size: 150 231 663 598 50 795
Fragments arranged by size:
795 663 598 231 150 50
ApaLI G'TGCA_C
Cuts at: 0 1849 2487
Size: 1849 638
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 683 716 2212 2245 2487
Size: 683 33 1496 33 242
Fragments arranged by size:
1496 683 242 33 33
BanI G'GyrC_C
Cuts at: 0 574 829 1308 1495 2202 2487
Size: 574 255 479 187 707 285
Fragments arranged by size:
707 574 479 285 255 187
BanII G_rGCy'C
Cuts at: 0 8 140 548 1649 2057 2380 2487
Size: 8 132 408 1101 408 323 107
Fragments arranged by size:
1101 408 408 323 132 107 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 315 462 1400 2085 2487
Size: 81 234 147 938 685 402
Fragments arranged by size:
938 685 402 234 147 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 1830 2487
Size: 1830 657
BclI T'GATC_A
Cuts at: 0 1895 2487
Size: 1895 592
BglII A'GATC_T
Cuts at: 0 281 2487
Size: 281 2206
Bme1580I G_kGCm'C
Cuts at: 0 1498 1853 2487
Size: 1498 355 634
Fragments arranged by size:
1498 634 355
BmgBI CAC'GTC
Cuts at: 0 1848 2199 2487
Size: 1848 351 288
Fragments arranged by size:
1848 351 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 1373 1630 2487
Size: 1373 257 857
Fragments arranged by size:
1373 857 257
BmtI G_CTAG'C
Cuts at: 0 1459 2487
Size: 1459 1028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 831 2185 2487
Size: 25 806 1354 302
Fragments arranged by size:
1354 806 302 25
Bpu10I CC'TnA_GC
Cuts at: 0 1643 2374 2487
Size: 1643 731 113
Fragments arranged by size:
1643 731 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2487
Size: 376 2111
BsaI GGTCTCn'nnnn_
Cuts at: 0 1433 2487
Size: 1433 1054
BsaAI yAC'GTr
Cuts at: 0 1290 2487
Size: 1290 1197
BsaBI GATnn'nnATC
Cuts at: 0 1660 2487
Size: 1660 827
BsaHI Gr'CG_yC
Cuts at: 0 1064 2363 2487
Size: 1064 1299 124
Fragments arranged by size:
1299 1064 124
BsaWI w'CCGG_w
Cuts at: 0 818 2487
Size: 818 1669
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1360 1390 2487
Size: 1360 30 1097
Fragments arranged by size:
1360 1097 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 541 2379 2487
Size: 22 519 1838 108
Fragments arranged by size:
1838 519 108 22
BseYI C'CCAG_C
Cuts at: 0 150 1443 1742 1977 2147 2487
Size: 150 1293 299 235 170 340
Fragments arranged by size:
1293 340 299 235 170 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 2487
Size: 1057 1430
BsiEI CG_ry'CG
Cuts at: 0 1506 2487
Size: 1506 981
BsiHKAI G_wGCw'C
Cuts at: 0 548 826 1853 2380 2487
Size: 548 278 1027 527 107
Fragments arranged by size:
1027 548 527 278 107
BsmI GAATG_Cn'
Cuts at: 0 1023 2487
Size: 1023 1464
BsmBI CGTCTCn'nnnn_
Cuts at: 0 32 2487
Size: 32 2455
Bsp1286I G_dGCh'C
Cuts at: 0 8 140 548 826 1498 1649 1853 2057
Size: 8 132 408 278 672 151 204 204
Cuts at: 2057 2380 2487
Size: 323 107
Fragments arranged by size:
672 408 323 278 204 204 151 132
107 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 696 1470 1945 2487
Size: 696 774 475 542
Fragments arranged by size:
774 696 542 475
BsrDI GCAATG_nn'
Cuts at: 0 2199 2487
Size: 2199 288
BssSI C'ACGA_G
Cuts at: 0 11 2487
Size: 11 2476
BstXI CCAn_nnnn'nTGG
Cuts at: 0 855 906 936 1281 1451 2487
Size: 855 51 30 345 170 1036
Fragments arranged by size:
1036 855 345 170 51 30
BstYI r'GATC_y
Cuts at: 0 281 950 2487
Size: 281 669 1537
Fragments arranged by size:
1537 669 281
Bsu36I CC'TnA_GG
Cuts at: 0 461 2487
Size: 461 2026
BtgI C'CryG_G
Cuts at: 0 716 929 1460 1559 1629 1869 1920 2487
Size: 716 213 531 99 70 240 51 567
Fragments arranged by size:
716 567 531 240 213 99 70 51
BtsI GCAGTG_nn'
Cuts at: 0 64 941 1028 1385 2487
Size: 64 877 87 357 1102
Fragments arranged by size:
1102 877 357 87 64
DraIII CAC_nnn'GTG
Cuts at: 0 561 1910 2487
Size: 561 1349 577
Fragments arranged by size:
1349 577 561
DrdI GACnn_nn'nnGTC
Cuts at: 0 1804 2487
Size: 1804 683
EaeI y'GGCC_r
Cuts at: 0 141 552 1556 1923 2239 2487
Size: 141 411 1004 367 316 248
Fragments arranged by size:
1004 411 367 316 248 141
EarI CTCTTCn'nnn_
Cuts at: 0 2102 2117 2487
Size: 2102 15 370
Fragments arranged by size:
2102 370 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2382 2487
Size: 2382 105
EcoICRI GAG'CTC
Cuts at: 0 546 2378 2487
Size: 546 1832 109
Fragments arranged by size:
1832 546 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 75 122 280 831 1273 1837 1849
Size: 25 50 47 158 551 442 564 12
Cuts at: 1849 2185 2305 2487
Size: 336 120 182
Fragments arranged by size:
564 551 442 336 182 158 120 50
47 25 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 1831 2487
Size: 40 1791 656
Fragments arranged by size:
1791 656 40
EcoO109I rG'GnC_Cy
Cuts at: 0 364 588 789 1239 1710 2487
Size: 364 224 201 450 471 777
Fragments arranged by size:
777 471 450 364 224 201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 384 2487
Size: 384 2103
HaeII r_GCGC'y
Cuts at: 0 151 2487
Size: 151 2336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 653 685 1949 1981 2353 2385
Size: 369 32 252 32 1264 32 372 32
Cuts at: 2385 2487
Size: 102
Fragments arranged by size:
1264 372 369 252 102 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1785 2487
Size: 1785 702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2384 2423 2487
Size: 2384 39 64
Fragments arranged by size:
2384 64 39
MscI TGG'CCA
Cuts at: 0 554 1558 1925 2487
Size: 554 1004 367 562
Fragments arranged by size:
1004 562 554 367
MslI CAynn'nnrTG
Cuts at: 0 610 904 934 1080 1279 1489 1925 2487
Size: 610 294 30 146 199 210 436 562
Fragments arranged by size:
610 562 436 294 210 199 146 30
MspA1I CmG'CkG
Cuts at: 0 1489 1887 2354 2487
Size: 1489 398 467 133
Fragments arranged by size:
1489 467 398 133
NcoI C'CATG_G
Cuts at: 0 1460 1559 1629 1869 1920 2487
Size: 1460 99 70 240 51 567
Fragments arranged by size:
1460 567 240 99 70 51
NdeI CA'TA_TG
Cuts at: 0 1842 2487
Size: 1842 645
NheI G'CTAG_C
Cuts at: 0 1455 2487
Size: 1455 1032
NspI r_CATG'y
Cuts at: 0 2466 2487
Size: 2466 21
PasI CC'CwG_GG
Cuts at: 0 175 2487
Size: 175 2312
PciI A'CATG_T
Cuts at: 0 2462 2487
Size: 2462 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 1474 2487
Size: 150 1324 1013
Fragments arranged by size:
1324 1013 150
PfoI T'CCnGG_A
Cuts at: 0 242 1346 2487
Size: 242 1104 1141
Fragments arranged by size:
1141 1104 242
PmlI CAC'GTG
Cuts at: 0 1290 2487
Size: 1290 1197
PpuMI rG'GwC_Cy
Cuts at: 0 364 588 789 1710 2487
Size: 364 224 201 921 777
Fragments arranged by size:
921 777 364 224 201
PsiI TTA'TAA
Cuts at: 0 2272 2487
Size: 2272 215
PstI C_TGCA'G
Cuts at: 0 59 1392 2487
Size: 59 1333 1095
Fragments arranged by size:
1333 1095 59
PvuII CAG'CTG
Cuts at: 0 1489 1887 2487
Size: 1489 398 600
Fragments arranged by size:
1489 600 398
SacI G_AGCT'C
Cuts at: 0 548 2380 2487
Size: 548 1832 107
Fragments arranged by size:
1832 548 107
SanDI GG'GwC_CC
Cuts at: 0 364 2487
Size: 364 2123
SexAI A'CCwGG_T
Cuts at: 0 687 2487
Size: 687 1800
SfcI C'TryA_G
Cuts at: 0 55 426 476 1388 2487
Size: 55 371 50 912 1099
Fragments arranged by size:
1099 912 371 55 50
SmlI C'TyrA_G
Cuts at: 0 391 2487
Size: 391 2096
StyI C'CwwG_G
Cuts at: 0 110 1460 1559 1629 1869 1920 2487
Size: 110 1350 99 70 240 51 567
Fragments arranged by size:
1350 567 240 110 99 70 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 631 1156 2161 2238 2487
Size: 315 316 525 1005 77 249
Fragments arranged by size:
1005 525 316 315 249 77
TatI w'GTAC_w
Cuts at: 0 878 2487
Size: 878 1609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 875 907 1360 1392 2487
Size: 875 32 453 32 1095
Fragments arranged by size:
1095 875 453 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2089 2487
Size: 2089 398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 933 1182 2178 2487
Size: 933 249 996 309
Fragments arranged by size:
996 933 309 249
XmnI GAAnn'nnTTC
Cuts at: 0 160 283 1822 2487
Size: 160 123 1539 665
Fragments arranged by size:
1539 665 160 123
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlwNI ApaLI
BaeI BanI BanII BbsI BciVI BclI BglII Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BssSI BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NcoI NdeI NheI
NspI PasI PciI PflMI PfoI PmlI PpuMI PsiI
PstI PvuII SacI SanDI SexAI SfcI SmlI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbvCI BcgI BfrBI BglI BlpI BplI BsiWI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NotI NruI NsiI
PacI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI ZraI