Restriction map for
pF1KB5789
(Linear) (Six-base) MAPSORT of: KIBB5789.seq Check: 7757 from: 1 to: 2487
KIBB5789.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
Acc65I G'GTAC_C
Cuts at: 0 412 2487
Size: 412 2075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 434 1079 1172 1269 2487
Size: 173 261 645 93 97 1218
Fragments arranged by size:
1218 645 261 173 97 93
AfeI AGC'GCT
Cuts at: 0 1941 2487
Size: 1941 546
AflIII A'CryG_T
Cuts at: 0 309 533 1294 2487
Size: 309 224 761 1193
Fragments arranged by size:
1193 761 309 224
AleI CACnn'nnGTG
Cuts at: 0 712 788 2487
Size: 712 76 1699
Fragments arranged by size:
1699 712 76
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2207 2239 2487
Size: 2207 32 248
Fragments arranged by size:
2207 248 32
AlwNI CAG_nnn'CTG
Cuts at: 0 223 1057 1131 1519 1541 1633 1866 2265
Size: 223 834 74 388 22 92 233 399
Cuts at: 2265 2487
Size: 222
Fragments arranged by size:
834 399 388 233 223 222 92 74
22
ApaI G_GGCC'C
Cuts at: 0 202 249 2487
Size: 202 47 2238
Fragments arranged by size:
2238 202 47
ApaLI G'TGCA_C
Cuts at: 0 340 802 1849 2487
Size: 340 462 1047 638
Fragments arranged by size:
1047 638 462 340
ApoI r'AATT_y
Cuts at: 0 105 927 1834 2487
Size: 105 822 907 653
Fragments arranged by size:
907 822 653 105
AvaI C'yCGr_G
Cuts at: 0 828 1393 1435 2487
Size: 828 565 42 1052
Fragments arranged by size:
1052 828 565 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1046 1079 2487
Size: 1046 33 1408
Fragments arranged by size:
1408 1046 33
BamHI G'GATC_C
Cuts at: 0 213 2487
Size: 213 2274
BanI G'GyrC_C
Cuts at: 0 366 412 1873 2147 2374 2487
Size: 366 46 1461 274 227 113
Fragments arranged by size:
1461 366 274 227 113 46
BanII G_rGCy'C
Cuts at: 0 202 249 399 564 924 1043 1138 1394
Size: 202 47 150 165 360 119 95 256
Cuts at: 1394 1442 2487
Size: 48 1045
Fragments arranged by size:
1045 360 256 202 165 150 119 95
48 47
BbeI G_GCGC'C
Cuts at: 0 1877 2151 2487
Size: 1877 274 336
Fragments arranged by size:
1877 336 274
BbvCI CC'TCA_GC
Cuts at: 0 571 2487
Size: 571 1916
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 962 2487
Size: 928 34 1525
Fragments arranged by size:
1525 928 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1911 2487
Size: 1911 576
BclI T'GATC_A
Cuts at: 0 1329 2487
Size: 1329 1158
BlpI GC'TnA_GC
Cuts at: 0 403 2487
Size: 403 2084
Bme1580I G_kGCm'C
Cuts at: 0 202 249 344 371 806 1731 1853 2012
Size: 202 47 95 27 435 925 122 159
Cuts at: 2012 2096 2471 2487
Size: 84 375 16
Fragments arranged by size:
925 435 375 202 159 122 95 84
47 27 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1854 2487
Size: 1854 633
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 527 1533 1854 2487
Size: 527 1006 321 633
Fragments arranged by size:
1006 633 527 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 497 565 628 807 835 895 979
Size: 313 184 68 63 179 28 60 84
Cuts at: 979 1655 2137 2487
Size: 676 482 350
Fragments arranged by size:
676 482 350 313 184 179 84 68
63 60 28
Bpu10I CC'TnA_GC
Cuts at: 0 502 571 1037 1132 1139 2487
Size: 502 69 466 95 7 1348
Fragments arranged by size:
1348 502 466 95 69 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 463 2487
Size: 365 98 2024
Fragments arranged by size:
2024 365 98
BsaAI yAC'GTr
Cuts at: 0 345 867 969 1295 2212 2487
Size: 345 522 102 326 917 275
Fragments arranged by size:
917 522 345 326 275 102
BsaHI Gr'CG_yC
Cuts at: 0 1874 2148 2487
Size: 1874 274 339
Fragments arranged by size:
1874 339 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 780 810 2290 2320 2487
Size: 780 30 1480 30 167
Fragments arranged by size:
1480 780 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 2487
Size: 274 2213
BseYI C'CCAG_C
Cuts at: 0 59 518 555 1203 1731 1877 1986 2065
Size: 59 459 37 648 528 146 109 79
Cuts at: 2065 2487
Size: 422
Fragments arranged by size:
648 528 459 422 146 109 79 59
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1615 1931 2010 2487
Size: 949 666 316 79 477
Fragments arranged by size:
949 666 477 316 79
BsiHKAI G_wGCw'C
Cuts at: 0 344 399 589 709 806 1043 1214 1853
Size: 344 55 190 120 97 237 171 639
Cuts at: 1853 2487
Size: 634
Fragments arranged by size:
639 634 344 237 190 171 120 97
55
BsmI GAATG_Cn'
Cuts at: 0 852 2487
Size: 852 1635
Bsp1286I G_dGCh'C
Cuts at: 0 202 249 344 371 399 564 589 709
Size: 202 47 95 27 28 165 25 120
Cuts at: 709 806 924 1043 1138 1214 1394 1442 1731
Size: 97 118 119 95 76 180 48 289
Cuts at: 1731 1853 2012 2096 2471 2487
Size: 122 159 84 375 16
Fragments arranged by size:
375 289 202 180 165 159 122 120
119 118 97 95 95 84 76 48
47 28 27 25 16
BspHI T'CATG_A
Cuts at: 0 1575 2487
Size: 1575 912
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 914 2487
Size: 914 1573
BsrFI r'CCGG_y
Cuts at: 0 1735 2487
Size: 1735 752
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1084 2310 2487
Size: 1084 1226 177
Fragments arranged by size:
1226 1084 177
BstEII G'GTnAC_C
Cuts at: 0 237 285 649 2487
Size: 237 48 364 1838
Fragments arranged by size:
1838 364 237 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 879 1263 2044 2487
Size: 249 630 384 781 443
Fragments arranged by size:
781 630 443 384 249
BstYI r'GATC_y
Cuts at: 0 213 483 2487
Size: 213 270 2004
Fragments arranged by size:
2004 270 213
Bsu36I CC'TnA_GG
Cuts at: 0 660 1145 1379 1786 2487
Size: 660 485 234 407 701
Fragments arranged by size:
701 660 485 407 234
BtgI C'CryG_G
Cuts at: 0 683 933 1256 1646 2043 2129 2228 2487
Size: 683 250 323 390 397 86 99 259
Fragments arranged by size:
683 397 390 323 259 250 99 86
BtsI GCAGTG_nn'
Cuts at: 0 674 1200 2311 2363 2487
Size: 674 526 1111 52 124
Fragments arranged by size:
1111 674 526 124 52
DraIII CAC_nnn'GTG
Cuts at: 0 1119 2487
Size: 1119 1368
DrdI GACnn_nn'nnGTC
Cuts at: 0 1024 1071 2487
Size: 1024 47 1416
Fragments arranged by size:
1416 1024 47
EaeI y'GGCC_r
Cuts at: 0 1253 1406 2084 2487
Size: 1253 153 678 403
Fragments arranged by size:
1253 678 403 153
EarI CTCTTCn'nnn_
Cuts at: 0 442 2487
Size: 442 2045
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1438 2487
Size: 1438 1049
EcoICRI GAG'CTC
Cuts at: 0 397 1041 2487
Size: 397 644 1446
Fragments arranged by size:
1446 644 397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 313 434 497 565 628 807 835
Size: 173 140 121 63 68 63 179 28
Cuts at: 835 895 979 1079 1172 1269 1655 2137 2487
Size: 60 84 100 93 97 386 482 350
Fragments arranged by size:
482 386 350 179 173 140 121 100
97 93 84 68 63 63 60 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 505 910 1378 2487
Size: 505 405 468 1109
Fragments arranged by size:
1109 505 468 405
EcoO109I rG'GnC_Cy
Cuts at: 0 198 245 246 614 832 1630 1974 2073
Size: 198 47 1 368 218 798 344 99
Cuts at: 2073 2442 2487
Size: 369 45
Fragments arranged by size:
798 369 368 344 218 198 99 47
45 1
EcoRI G'AATT_C
Cuts at: 0 927 2487
Size: 927 1560
EcoRV GAT'ATC
Cuts at: 0 1323 2487
Size: 1323 1164
FspI TGC'GCA
Cuts at: 0 2314 2487
Size: 2314 173
HaeII r_GCGC'y
Cuts at: 0 1877 1943 2151 2487
Size: 1877 66 208 336
Fragments arranged by size:
1877 336 208 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 482 514 643 675 1523 1555 2268 2300
Size: 482 32 129 32 848 32 713 32
Cuts at: 2300 2487
Size: 187
Fragments arranged by size:
848 713 482 187 129 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 1873 2147 2487
Size: 1873 274 340
Fragments arranged by size:
1873 340 274
KpnI G_GTAC'C
Cuts at: 0 416 2487
Size: 416 2071
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1670 1790 2487
Size: 1670 120 697
Fragments arranged by size:
1670 697 120
MscI TGG'CCA
Cuts at: 0 1255 1408 2086 2487
Size: 1255 153 678 401
Fragments arranged by size:
1255 678 401 153
MslI CAynn'nnrTG
Cuts at: 0 712 788 2487
Size: 712 76 1699
Fragments arranged by size:
1699 712 76
MspA1I CmG'CkG
Cuts at: 0 135 543 624 2487
Size: 135 408 81 1863
Fragments arranged by size:
1863 408 135 81
NaeI GCC'GGC
Cuts at: 0 1737 2487
Size: 1737 750
NarI GG'CG_CC
Cuts at: 0 1874 2148 2487
Size: 1874 274 339
Fragments arranged by size:
1874 339 274
NcoI C'CATG_G
Cuts at: 0 933 1256 2043 2487
Size: 933 323 787 444
Fragments arranged by size:
933 787 444 323
NgoMIV G'CCGG_C
Cuts at: 0 1735 2487
Size: 1735 752
NspI r_CATG'y
Cuts at: 0 313 537 1528 2313 2340 2487
Size: 313 224 991 785 27 147
Fragments arranged by size:
991 785 313 224 147 27
PciI A'CATG_T
Cuts at: 0 309 533 2487
Size: 309 224 1954
Fragments arranged by size:
1954 309 224
PflMI CCAn_nnn'nTGG
Cuts at: 0 223 606 627 1519 1633 2487
Size: 223 383 21 892 114 854
Fragments arranged by size:
892 854 383 223 114 21
PmlI CAC'GTG
Cuts at: 0 345 969 1295 2212 2487
Size: 345 624 326 917 275
Fragments arranged by size:
917 624 345 326 275
PpuMI rG'GwC_Cy
Cuts at: 0 614 832 2073 2442 2487
Size: 614 218 1241 369 45
Fragments arranged by size:
1241 614 369 218 45
PspOMI G'GGCC_C
Cuts at: 0 198 245 2487
Size: 198 47 2242
Fragments arranged by size:
2242 198 47
PspXI vC'TCGA_Gb
Cuts at: 0 1435 2487
Size: 1435 1052
PstI C_TGCA'G
Cuts at: 0 69 1871 2487
Size: 69 1802 616
Fragments arranged by size:
1802 616 69
PvuII CAG'CTG
Cuts at: 0 543 624 2487
Size: 543 81 1863
Fragments arranged by size:
1863 543 81
SacI G_AGCT'C
Cuts at: 0 399 1043 2487
Size: 399 644 1444
Fragments arranged by size:
1444 644 399
SanDI GG'GwC_CC
Cuts at: 0 832 2487
Size: 832 1655
SbfI CC_TGCA'GG
Cuts at: 0 1871 2487
Size: 1871 616
SexAI A'CCwGG_T
Cuts at: 0 362 1050 2487
Size: 362 688 1437
Fragments arranged by size:
1437 688 362
SfcI C'TryA_G
Cuts at: 0 65 280 390 1867 2103 2487
Size: 65 215 110 1477 236 384
Fragments arranged by size:
1477 384 236 215 110 65
SfoI GGC'GCC
Cuts at: 0 1875 2149 2487
Size: 1875 274 338
Fragments arranged by size:
1875 338 274
SmaI CCC'GGG
Cuts at: 0 830 1395 2487
Size: 830 565 1092
Fragments arranged by size:
1092 830 565
SmlI C'TyrA_G
Cuts at: 0 380 442 1435 2487
Size: 380 62 993 1052
Fragments arranged by size:
1052 993 380 62
SphI G_CATG'C
Cuts at: 0 2313 2340 2487
Size: 2313 27 147
Fragments arranged by size:
2313 147 27
StuI AGG'CCT
Cuts at: 0 420 658 2487
Size: 420 238 1829
Fragments arranged by size:
1829 420 238
StyI C'CwwG_G
Cuts at: 0 53 578 933 1256 2043 2115 2186 2487
Size: 53 525 355 323 787 72 71 301
Fragments arranged by size:
787 525 355 323 301 72 71 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 269 637 702 2190 2431 2487
Size: 269 368 65 1488 241 56
Fragments arranged by size:
1488 368 269 241 65 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 780 812 2103 2135 2290 2322 2487
Size: 780 32 1291 32 155 32 165
Fragments arranged by size:
1291 780 165 155 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2427 2487
Size: 2427 60
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 2161 2487
Size: 681 1480 326
Fragments arranged by size:
1480 681 326
XhoI C'TCGA_G
Cuts at: 0 1435 2487
Size: 1435 1052
XmaI C'CCGG_G
Cuts at: 0 828 1393 2487
Size: 828 565 1094
Fragments arranged by size:
1094 828 565
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmlI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SbfI SexAI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AlfI
AscI AseI AsiSI AvrII BbsI BfrBI BglI BglII
BmgBI BmtI BsaI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraI EagI FalI FseI FspAI
HincII HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacII SalI SapI ScaI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI XbaI XmnI ZraI