Restriction map for
pF1KB5791
(Linear) (Six-base) MAPSORT of: KIBB5791.seq Check: 6765 from: 1 to: 2490
KIBB5791.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 553 1438 1798 2490
Size: 553 885 360 692
Fragments arranged by size:
885 692 553 360
Acc65I G'GTAC_C
Cuts at: 0 698 1406 2490
Size: 698 708 1084
Fragments arranged by size:
1084 708 698
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 814 852 1271 2048 2154 2490
Size: 60 754 38 419 777 106 336
Fragments arranged by size:
777 754 419 336 106 60 38
AflIII A'CryG_T
Cuts at: 0 494 621 1603 2121 2490
Size: 494 127 982 518 369
Fragments arranged by size:
982 518 494 369 127
AleI CACnn'nnGTG
Cuts at: 0 1695 2490
Size: 1695 795
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1552 2490
Size: 1552 938
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 596 628 2490
Size: 596 32 1862
Fragments arranged by size:
1862 596 32
AlwNI CAG_nnn'CTG
Cuts at: 0 424 978 2490
Size: 424 554 1512
Fragments arranged by size:
1512 554 424
ApaI G_GGCC'C
Cuts at: 0 716 1832 2490
Size: 716 1116 658
Fragments arranged by size:
1116 716 658
ApoI r'AATT_y
Cuts at: 0 56 1051 1494 1771 2490
Size: 56 995 443 277 719
Fragments arranged by size:
995 719 443 277 56
AvaI C'yCGr_G
Cuts at: 0 523 924 1370 2481 2490
Size: 523 401 446 1111 9
Fragments arranged by size:
1111 523 446 401 9
AvrII C'CTAG_G
Cuts at: 0 2442 2490
Size: 2442 48
BamHI G'GATC_C
Cuts at: 0 278 2490
Size: 278 2212
BanI G'GyrC_C
Cuts at: 0 698 1396 1406 2490
Size: 698 698 10 1084
Fragments arranged by size:
1084 698 698 10
BanII G_rGCy'C
Cuts at: 0 614 716 1832 2490
Size: 614 102 1116 658
Fragments arranged by size:
1116 658 614 102
BglI GCCn_nnn'nGGC
Cuts at: 0 1067 2490
Size: 1067 1423
BlpI GC'TnA_GC
Cuts at: 0 1540 2490
Size: 1540 950
Bme1580I G_kGCm'C
Cuts at: 0 84 716 787 1717 1832 2490
Size: 84 632 71 930 115 658
Fragments arranged by size:
930 658 632 115 84 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 357 774 943 1315 1348 2490
Size: 357 417 169 372 33 1142
Fragments arranged by size:
1142 417 372 357 169 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 2490
Size: 538 1952
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1026 1186 1490 1843 1895 2072 2490
Size: 1026 160 304 353 52 177 418
Fragments arranged by size:
1026 418 353 304 177 160 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1227 2490
Size: 628 599 1263
Fragments arranged by size:
1263 628 599
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 2490
Size: 520 1970
BsaAI yAC'GTr
Cuts at: 0 588 624 2490
Size: 588 36 1866
Fragments arranged by size:
1866 588 36
BsaBI GATnn'nnATC
Cuts at: 0 277 403 2490
Size: 277 126 2087
Fragments arranged by size:
2087 277 126
BsaWI w'CCGG_w
Cuts at: 0 900 2490
Size: 900 1590
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 244 274 466 496 532 562 596 626
Size: 244 30 192 30 36 30 34 30
Cuts at: 626 1448 1478 2490
Size: 822 30 1012
Fragments arranged by size:
1012 822 244 192 36 34 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1283 2490
Size: 1283 1207
BseYI C'CCAG_C
Cuts at: 0 715 942 2490
Size: 715 227 1548
Fragments arranged by size:
1548 715 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 696 985 1109 1512 2213 2490
Size: 672 24 289 124 403 701 277
Fragments arranged by size:
701 672 403 289 277 124 24
BsiEI CG_ry'CG
Cuts at: 0 1669 2490
Size: 1669 821
BsiHKAI G_wGCw'C
Cuts at: 0 446 614 629 2490
Size: 446 168 15 1861
Fragments arranged by size:
1861 446 168 15
BsmI GAATG_Cn'
Cuts at: 0 733 841 1516 1849 2490
Size: 733 108 675 333 641
Fragments arranged by size:
733 675 641 333 108
Bsp1286I G_dGCh'C
Cuts at: 0 84 446 614 629 716 787 1717 1832
Size: 84 362 168 15 87 71 930 115
Cuts at: 1832 2490
Size: 658
Fragments arranged by size:
930 658 362 168 115 87 84 71
15
BspHI T'CATG_A
Cuts at: 0 629 2490
Size: 629 1861
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 553 829 1438 1798 1891 2287 2490
Size: 553 276 609 360 93 396 203
Fragments arranged by size:
609 553 396 360 276 203 93
BsrBI CCG'CTC
Cuts at: 0 1020 2332 2490
Size: 1020 1312 158
Fragments arranged by size:
1312 1020 158
BsrDI GCAATG_nn'
Cuts at: 0 919 1440 1927 2490
Size: 919 521 487 563
Fragments arranged by size:
919 563 521 487
BssSI C'ACGA_G
Cuts at: 0 1763 2490
Size: 1763 727
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1193 2490
Size: 1193 1297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 709 723 1839 2251 2490
Size: 709 14 1116 412 239
Fragments arranged by size:
1116 709 412 239 14
BstYI r'GATC_y
Cuts at: 0 278 1347 2490
Size: 278 1069 1143
Fragments arranged by size:
1143 1069 278
BtgI C'CryG_G
Cuts at: 0 1001 1179 2490
Size: 1001 178 1311
Fragments arranged by size:
1311 1001 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2155 2490
Size: 2155 335
BtsI GCAGTG_nn'
Cuts at: 0 444 679 684 973 1022 1345 2490
Size: 444 235 5 289 49 323 1145
Fragments arranged by size:
1145 444 323 289 235 49 5
EaeI y'GGCC_r
Cuts at: 0 2 203 2253 2490
Size: 2 201 2050 237
Fragments arranged by size:
2050 237 201 2
EarI CTCTTCn'nnn_
Cuts at: 0 872 2490
Size: 872 1618
EcoICRI GAG'CTC
Cuts at: 0 612 2490
Size: 612 1878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 814 852 1026 1186 1271 1490 1843
Size: 60 754 38 174 160 85 219 353
Cuts at: 1843 1895 2048 2072 2154 2490
Size: 52 153 24 82 336
Fragments arranged by size:
754 353 336 219 174 160 153 85
82 60 52 38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 806 1910 2490
Size: 238 568 1104 580
Fragments arranged by size:
1104 580 568 238
EcoO109I rG'GnC_Cy
Cuts at: 0 243 1828 2490
Size: 243 1585 662
Fragments arranged by size:
1585 662 243
EcoRI G'AATT_C
Cuts at: 0 1494 1771 2490
Size: 1494 277 719
Fragments arranged by size:
1494 719 277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2015 2047 2490
Size: 2015 32 443
Fragments arranged by size:
2015 443 32
HincII GTy'rAC
Cuts at: 0 2264 2490
Size: 2264 226
HindIII A'AGCT_T
Cuts at: 0 1331 2490
Size: 1331 1159
KpnI G_GTAC'C
Cuts at: 0 702 1410 2490
Size: 702 708 1080
Fragments arranged by size:
1080 708 702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 876 2177 2490
Size: 876 1301 313
Fragments arranged by size:
1301 876 313
MscI TGG'CCA
Cuts at: 0 4 205 2255 2490
Size: 4 201 2050 235
Fragments arranged by size:
2050 235 201 4
MslI CAynn'nnrTG
Cuts at: 0 153 303 1695 2490
Size: 153 150 1392 795
Fragments arranged by size:
1392 795 153 150
MspA1I CmG'CkG
Cuts at: 0 1046 1100 1164 1244 2054 2490
Size: 1046 54 64 80 810 436
Fragments arranged by size:
1046 810 436 80 64 54
NcoI C'CATG_G
Cuts at: 0 1001 2490
Size: 1001 1489
NdeI CA'TA_TG
Cuts at: 0 2451 2490
Size: 2451 39
NspI r_CATG'y
Cuts at: 0 498 1095 1607 2096 2125 2243 2490
Size: 498 597 512 489 29 118 247
Fragments arranged by size:
597 512 498 489 247 118 29
PciI A'CATG_T
Cuts at: 0 494 1603 2121 2490
Size: 494 1109 518 369
Fragments arranged by size:
1109 518 494 369
PflMI CCAn_nnn'nTGG
Cuts at: 0 45 648 2159 2490
Size: 45 603 1511 331
Fragments arranged by size:
1511 603 331 45
PfoI T'CCnGG_A
Cuts at: 0 1974 2303 2490
Size: 1974 329 187
Fragments arranged by size:
1974 329 187
PmlI CAC'GTG
Cuts at: 0 624 2490
Size: 624 1866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 596 628 2490
Size: 596 32 1862
Fragments arranged by size:
1862 596 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 2490
Size: 243 2247
PshAI GACnn'nnGTC
Cuts at: 0 1119 2483 2490
Size: 1119 1364 7
Fragments arranged by size:
1364 1119 7
PsiI TTA'TAA
Cuts at: 0 1595 2490
Size: 1595 895
PspOMI G'GGCC_C
Cuts at: 0 712 1828 2490
Size: 712 1116 662
Fragments arranged by size:
1116 712 662
PstI C_TGCA'G
Cuts at: 0 505 640 865 1423 2059 2490
Size: 505 135 225 558 636 431
Fragments arranged by size:
636 558 505 431 225 135
PvuI CG_AT'CG
Cuts at: 0 1669 2490
Size: 1669 821
PvuII CAG'CTG
Cuts at: 0 1046 2054 2490
Size: 1046 1008 436
Fragments arranged by size:
1046 1008 436
SacI G_AGCT'C
Cuts at: 0 614 2490
Size: 614 1876
SfcI C'TryA_G
Cuts at: 0 255 501 636 861 1071 1233 1419 1468
Size: 255 246 135 225 210 162 186 49
Cuts at: 1468 1962 2055 2490
Size: 494 93 435
Fragments arranged by size:
494 435 255 246 225 210 186 162
135 93 49
SmaI CCC'GGG
Cuts at: 0 1372 2490
Size: 1372 1118
SmlI C'TyrA_G
Cuts at: 0 523 607 1206 2490
Size: 523 84 599 1284
Fragments arranged by size:
1284 599 523 84
SphI G_CATG'C
Cuts at: 0 2096 2243 2490
Size: 2096 147 247
Fragments arranged by size:
2096 247 147
SspI AAT'ATT
Cuts at: 0 162 2490
Size: 162 2328
StyI C'CwwG_G
Cuts at: 0 1001 2442 2490
Size: 1001 1441 48
Fragments arranged by size:
1441 1001 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 297 2350 2490
Size: 297 2053 140
Fragments arranged by size:
2053 297 140
TatI w'GTAC_w
Cuts at: 0 1660 1958 2490
Size: 1660 298 532
Fragments arranged by size:
1660 532 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 890 922 1448 1480 2490
Size: 890 32 526 32 1010
Fragments arranged by size:
1010 890 526 32 32
XbaI T'CTAG_A
Cuts at: 0 2202 2490
Size: 2202 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2218 2490
Size: 2218 272
XhoI C'TCGA_G
Cuts at: 0 523 2490
Size: 523 1967
XmaI C'CCGG_G
Cuts at: 0 1370 2490
Size: 1370 1120
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApoI AvaI AvrII BamHI BanI BanII BglI
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NcoI NdeI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SfcI SmaI SmlI SphI
SspI StyI TaqII TatI TstI XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI ApaLI
AscI AseI AsiSI BaeI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I BsaHI
BsiWI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PspXI PsrI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI
Tth111I XmnI ZraI