Restriction map for pF1KB5796
 (Linear) (Six-base) MAPSORT of: KIBB5796.seq  Check: 7614  from: 1  to: 2340



KIBB5796.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1430    2179    2316    2340 
   Size:       1430     749     137      24

  Fragments arranged by size: 

                1430     749     137      24
 
AfeI AGC'GCT

Cuts at:      0    1834    2340 
   Size:       1834     506
 
AflIII A'CryG_T

Cuts at:      0    1116    2340 
   Size:       1116    1224
 
AleI CACnn'nnGTG

Cuts at:      0     158     506     524     806     821    2340 
   Size:        158     348      18     282      15    1519

  Fragments arranged by size: 

                1519     348     282     158      18      15
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2200    2232    2340 
   Size:       2200      32     108

  Fragments arranged by size: 

                2200     108      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      19    1272    2340 
   Size:         19    1253    1068

  Fragments arranged by size: 

                1253    1068      19
 
ApaI G_GGCC'C

Cuts at:      0    1659    2340 
   Size:       1659     681
 
ApoI r'AATT_y

Cuts at:      0     471    2340 
   Size:        471    1869
 
BamHI G'GATC_C

Cuts at:      0    1628    2075    2340 
   Size:       1628     447     265

  Fragments arranged by size: 

                1628     447     265
 
BanI G'GyrC_C

Cuts at:      0     307     884    1927    2149    2340 
   Size:        307     577    1043     222     191

  Fragments arranged by size: 

                1043     577     307     222     191
 
BanII G_rGCy'C

Cuts at:      0     117     270     356     605    1075    1659    2114    2129 
   Size:        117     153      86     249     470     584     455      15

Cuts at:   2129    2213    2340 
   Size:         84     127

  Fragments arranged by size: 

                 584     470     455     249     153     127     117      86
                  84      15
 
BbeI G_GCGC'C

Cuts at:      0     311    1931    2340 
   Size:        311    1620     409

  Fragments arranged by size: 

                1620     409     311
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1035    1181    1632    2102    2340 
   Size:       1035     146     451     470     238

  Fragments arranged by size: 

                1035     470     451     238     146
 
BclI T'GATC_A

Cuts at:      0     810    2340 
   Size:        810    1530
 
BglI GCCn_nnn'nGGC

Cuts at:      0     281    1663    2340 
   Size:        281    1382     677

  Fragments arranged by size: 

                1382     677     281
 
BlpI GC'TnA_GC

Cuts at:      0    1687    2340 
   Size:       1687     653
 
Bme1580I G_kGCm'C

Cuts at:      0     152     500    1659    1809    1923    2340 
   Size:        152     348    1159     150     114     417

  Fragments arranged by size: 

                1159     417     348     152     150     114
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     815    1188    2340 
   Size:        815     373    1152

  Fragments arranged by size: 

                1152     815     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1083    1210    2339    2340 
   Size:       1083     127    1129       1

  Fragments arranged by size: 

                1129    1083     127       1
 
Bpu10I CC'TnA_GC

Cuts at:      0     595    2340 
   Size:        595    1745
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287    1474    1618    2065    2340 
   Size:        287    1187     144     447     275

  Fragments arranged by size: 

                1187     447     287     275     144
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     443     727    2340 
   Size:        443     284    1613

  Fragments arranged by size: 

                1613     443     284
 
BsaAI yAC'GTr

Cuts at:      0    1056    1117    1473    2340 
   Size:       1056      61     356     867

  Fragments arranged by size: 

                1056     867     356      61
 
BsaHI Gr'CG_yC

Cuts at:      0     308    1928    2340 
   Size:        308    1620     412

  Fragments arranged by size: 

                1620     412     308
 
BsaWI w'CCGG_w

Cuts at:      0     180    1319    1625    2340 
   Size:        180    1139     306     715

  Fragments arranged by size: 

                1139     715     306     180
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2200    2230    2340 
   Size:       2200      30     110

  Fragments arranged by size: 

                2200     110      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     710    2340 
   Size:        710    1630
 
BseYI C'CCAG_C

Cuts at:      0     620    2340 
   Size:        620    1720
 
BsiEI CG_ry'CG

Cuts at:      0    1221    2340 
   Size:       1221    1119
 
BsiHKAI G_wGCw'C

Cuts at:      0     117     356     964    2114    2213    2340 
   Size:        117     239     608    1150      99     127

  Fragments arranged by size: 

                1150     608     239     127     117      99
 
Bsp1286I G_dGCh'C

Cuts at:      0     117     152     270     356     500     605     964    1075 
   Size:        117      35     118      86     144     105     359     111

Cuts at:   1075    1659    1809    1923    2114    2129    2213    2340 
   Size:        584     150     114     191      15      84     127

  Fragments arranged by size: 

                 584     359     191     150     144     127     118     117
                 114     111     105      86      84      35      15
 
BspEI T'CCGG_A

Cuts at:      0     180    1319    1625    2340 
   Size:        180    1139     306     715

  Fragments arranged by size: 

                1139     715     306     180
 
BspHI T'CATG_A

Cuts at:      0     378     693    2340 
   Size:        378     315    1647

  Fragments arranged by size: 

                1647     378     315
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     671    1783    2234    2340 
   Size:        671    1112     451     106

  Fragments arranged by size: 

                1112     671     451     106
 
BsrBI CCG'CTC

Cuts at:      0    1112    2270    2340 
   Size:       1112    1158      70

  Fragments arranged by size: 

                1158    1112      70
 
BsrFI r'CCGG_y

Cuts at:      0     881    1216    1220    2340 
   Size:        881     335       4    1120

  Fragments arranged by size: 

                1120     881     335       4
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     160     364     397     526     676     709    2136    2340 
   Size:        160     204      33     129     150      33    1427     204

  Fragments arranged by size: 

                1427     204     204     160     150     129      33      33
            
 
BstYI r'GATC_y

Cuts at:      0    1418    1628    2075    2340 
   Size:       1418     210     447     265

  Fragments arranged by size: 

                1418     447     265     210
 
Bsu36I CC'TnA_GG

Cuts at:      0    1660    2340 
   Size:       1660     680
 
BtgI C'CryG_G

Cuts at:      0     270     444     504     522     543     642     675     834 
   Size:        270     174      60      18      21      99      33     159

Cuts at:    834     915     942    1066    1559    1793    2340 
   Size:         81      27     124     493     234     547

  Fragments arranged by size: 

                 547     493     270     234     174     159     124      99
                  81      60      33      27      21      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1482    1731    2340 
   Size:       1482     249     609

  Fragments arranged by size: 

                1482     609     249
 
ClaI AT'CG_AT

Cuts at:      0    2288    2340 
   Size:       2288      52
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     267     302    2340 
   Size:        267      35    2038

  Fragments arranged by size: 

                2038     267      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     147    2340 
   Size:        147    2193
 
EaeI y'GGCC_r

Cuts at:      0    1218    1556    1562    2340 
   Size:       1218     338       6     778

  Fragments arranged by size: 

                1218     778     338       6
 
EagI C'GGCC_G

Cuts at:      0    1218    2340 
   Size:       1218    1122
 
EarI CTCTTCn'nnn_

Cuts at:      0    1267    1627    1786    1792    1873    2318    2340 
   Size:       1267     360     159       6      81     445      22

  Fragments arranged by size: 

                1267     445     360     159      81      22       6
 
EcoICRI GAG'CTC

Cuts at:      0     115     354    2112    2211    2340 
   Size:        115     239    1758      99     129

  Fragments arranged by size: 

                1758     239     129     115      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1083    1210    1430    2179    2316    2339    2340 
   Size:       1083     127     220     749     137      23       1

  Fragments arranged by size: 

                1083     749     220     137     127      23       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1297    2340 
   Size:       1297    1043
 
EcoO109I rG'GnC_Cy

Cuts at:      0     386     530     683     761    1656    1674    1721    2340 
   Size:        386     144     153      78     895      18      47     619

  Fragments arranged by size: 

                 895     619     386     153     144      78      47      18
            
 
EcoRV GAT'ATC

Cuts at:      0     779    1155    2340 
   Size:        779     376    1185

  Fragments arranged by size: 

                1185     779     376
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1305    2340 
   Size:       1305    1035
 
HaeII r_GCGC'y

Cuts at:      0     311    1836    1931    2032    2340 
   Size:        311    1525      95     101     308

  Fragments arranged by size: 

                1525     311     308     101      95
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     460     492    1879    1911    2340 
   Size:         27     433      32    1387      32     429

  Fragments arranged by size: 

                1387     433     429      32      32      27
 
KasI G'GCGC_C

Cuts at:      0     307    1927    2340 
   Size:        307    1620     413

  Fragments arranged by size: 

                1620     413     307
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     381     630    2340 
   Size:        381     249    1710

  Fragments arranged by size: 

                1710     381     249
 
MscI TGG'CCA

Cuts at:      0    1558    2340 
   Size:       1558     782
 
MslI CAynn'nnrTG

Cuts at:      0     158     380     506     524     674     707     806     821 
   Size:        158     222     126      18     150      33      99      15

Cuts at:    821    1126    1384    1390    1471    1564    1765    1906    2340 
   Size:        305     258       6      81      93     201     141     434

  Fragments arranged by size: 

                 434     305     258     222     201     158     150     141
                 126      99      93      81      33      18      15       6
            
 
MspA1I CmG'CkG

Cuts at:      0    1230    1795    2232    2340 
   Size:       1230     565     437     108

  Fragments arranged by size: 

                1230     565     437     108
 
NaeI GCC'GGC

Cuts at:      0    1222    2340 
   Size:       1222    1118
 
NarI GG'CG_CC

Cuts at:      0     308    1928    2340 
   Size:        308    1620     412

  Fragments arranged by size: 

                1620     412     308
 
NcoI C'CATG_G

Cuts at:      0     270     444     522     543     675     834     942    1559 
   Size:        270     174      78      21     132     159     108     617

Cuts at:   1559    2340 
   Size:        781

  Fragments arranged by size: 

                 781     617     270     174     159     132     108      78
                  21
 
NgoMIV G'CCGG_C

Cuts at:      0    1220    2340 
   Size:       1220    1120
 
PasI CC'CwG_GG

Cuts at:      0    1503    1725    1923    2340 
   Size:       1503     222     198     417

  Fragments arranged by size: 

                1503     417     222     198
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1338    2340 
   Size:       1338    1002
 
PfoI T'CCnGG_A

Cuts at:      0     991    1307    1706    2340 
   Size:        991     316     399     634

  Fragments arranged by size: 

                 991     634     399     316
 
PmlI CAC'GTG

Cuts at:      0    1117    2340 
   Size:       1117    1223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2200    2232    2340 
   Size:       2200      32     108

  Fragments arranged by size: 

                2200     108      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     386     530     683     761    1674    1721    2340 
   Size:        386     144     153      78     913      47     619

  Fragments arranged by size: 

                 913     619     386     153     144      78      47
 
PshAI GACnn'nnGTC

Cuts at:      0    1702    2340 
   Size:       1702     638
 
PspOMI G'GGCC_C

Cuts at:      0    1655    2340 
   Size:       1655     685
 
PstI C_TGCA'G

Cuts at:      0    2124    2231    2340 
   Size:       2124     107     109

  Fragments arranged by size: 

                2124     109     107
 
PvuII CAG'CTG

Cuts at:      0    2232    2340 
   Size:       2232     108
 
SacI G_AGCT'C

Cuts at:      0     117     356    2114    2213    2340 
   Size:        117     239    1758      99     127

  Fragments arranged by size: 

                1758     239     127     117      99
 
SacII CC_GC'GG

Cuts at:      0    1796    2340 
   Size:       1796     544
 
SanDI GG'GwC_CC

Cuts at:      0     386    1721    2340 
   Size:        386    1335     619

  Fragments arranged by size: 

                1335     619     386
 
ScaI AGT'ACT

Cuts at:      0    1975    2340 
   Size:       1975     365
 
SexAI A'CCwGG_T

Cuts at:      0    1991    2340 
   Size:       1991     349
 
SfcI C'TryA_G

Cuts at:      0     558    2120    2227    2340 
   Size:        558    1562     107     113

  Fragments arranged by size: 

                1562     558     113     107
 
SfoI GGC'GCC

Cuts at:      0     309    1929    2340 
   Size:        309    1620     411

  Fragments arranged by size: 

                1620     411     309
 
SmlI C'TyrA_G

Cuts at:      0     302    1489    1633    2044    2340 
   Size:        302    1187     144     411     296

  Fragments arranged by size: 

                1187     411     302     296     144
 
StuI AGG'CCT

Cuts at:      0    2042    2340 
   Size:       2042     298
 
StyI C'CwwG_G

Cuts at:      0     270     444     522     543     675     804     834     942 
   Size:        270     174      78      21     132     129      30     108

Cuts at:    942    1337    1445    1559    2340 
   Size:        395     108     114     781

  Fragments arranged by size: 

                 781     395     270     174     132     129     114     108
                 108      78      30      21
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1079    1082    1138    2340 
   Size:       1079       3      56    1202

  Fragments arranged by size: 

                1202    1079      56       3
 
TatI w'GTAC_w

Cuts at:      0    1973    2340 
   Size:       1973     367
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     601     633    2340 
   Size:        601      32    1707

  Fragments arranged by size: 

                1707     601      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     952    1092    2340 
   Size:        952     140    1248

  Fragments arranged by size: 

                1248     952     140
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     487    1566    2340 
   Size:        487    1079     774

  Fragments arranged by size: 

                1079     774     487
 
XmnI GAAnn'nnTTC

Cuts at:      0    1846    2340 
   Size:       1846     494
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     AleI     AloI    AlwNI     ApaI     ApoI
    BamHI     BanI    BanII     BbeI     BbsI     BclI     BglI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SacI    SacII    SanDI     ScaI    SexAI     SfcI
     SfoI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AlfI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BbvCI     BcgI    BciVI    BfrBI    BglII    BmgBI     BmtI     BplI
    BsaBI     BsgI    BsiWI     BsmI    BsmBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtsI     DraI     DrdI
     EciI    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PsiI    PspXI     PsrI     PvuI
    RsrII     SalI     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XhoI
     XmaI     ZraI
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