(Linear) (Six-base) MAPSORT of: KIBB5796.seq Check: 7614 from: 1 to: 2340 KIBB5796.orf With 182 enzymes: SgfI * October 7, 2009 14:17 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1430 2179 2316 2340 Size: 1430 749 137 24 Fragments arranged by size: 1430 749 137 24 AfeI AGC'GCT Cuts at: 0 1834 2340 Size: 1834 506 AflIII A'CryG_T Cuts at: 0 1116 2340 Size: 1116 1224 AleI CACnn'nnGTG Cuts at: 0 158 506 524 806 821 2340 Size: 158 348 18 282 15 1519 Fragments arranged by size: 1519 348 282 158 18 15 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2200 2232 2340 Size: 2200 32 108 Fragments arranged by size: 2200 108 32 AlwNI CAG_nnn'CTG Cuts at: 0 19 1272 2340 Size: 19 1253 1068 Fragments arranged by size: 1253 1068 19 ApaI G_GGCC'C Cuts at: 0 1659 2340 Size: 1659 681 ApoI r'AATT_y Cuts at: 0 471 2340 Size: 471 1869 BamHI G'GATC_C Cuts at: 0 1628 2075 2340 Size: 1628 447 265 Fragments arranged by size: 1628 447 265 BanI G'GyrC_C Cuts at: 0 307 884 1927 2149 2340 Size: 307 577 1043 222 191 Fragments arranged by size: 1043 577 307 222 191 BanII G_rGCy'C Cuts at: 0 117 270 356 605 1075 1659 2114 2129 Size: 117 153 86 249 470 584 455 15 Cuts at: 2129 2213 2340 Size: 84 127 Fragments arranged by size: 584 470 455 249 153 127 117 86 84 15 BbeI G_GCGC'C Cuts at: 0 311 1931 2340 Size: 311 1620 409 Fragments arranged by size: 1620 409 311 BbsI GAAGACnn'nnnn_ Cuts at: 0 1035 1181 1632 2102 2340 Size: 1035 146 451 470 238 Fragments arranged by size: 1035 470 451 238 146 BclI T'GATC_A Cuts at: 0 810 2340 Size: 810 1530 BglI GCCn_nnn'nGGC Cuts at: 0 281 1663 2340 Size: 281 1382 677 Fragments arranged by size: 1382 677 281 BlpI GC'TnA_GC Cuts at: 0 1687 2340 Size: 1687 653 Bme1580I G_kGCm'C Cuts at: 0 152 500 1659 1809 1923 2340 Size: 152 348 1159 150 114 417 Fragments arranged by size: 1159 417 348 152 150 114 BmrI ACTGGGnnnn_n' Cuts at: 0 815 1188 2340 Size: 815 373 1152 Fragments arranged by size: 1152 815 373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1083 1210 2339 2340 Size: 1083 127 1129 1 Fragments arranged by size: 1129 1083 127 1 Bpu10I CC'TnA_GC Cuts at: 0 595 2340 Size: 595 1745 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 287 1474 1618 2065 2340 Size: 287 1187 144 447 275 Fragments arranged by size: 1187 447 287 275 144 BsaI GGTCTCn'nnnn_ Cuts at: 0 443 727 2340 Size: 443 284 1613 Fragments arranged by size: 1613 443 284 BsaAI yAC'GTr Cuts at: 0 1056 1117 1473 2340 Size: 1056 61 356 867 Fragments arranged by size: 1056 867 356 61 BsaHI Gr'CG_yC Cuts at: 0 308 1928 2340 Size: 308 1620 412 Fragments arranged by size: 1620 412 308 BsaWI w'CCGG_w Cuts at: 0 180 1319 1625 2340 Size: 180 1139 306 715 Fragments arranged by size: 1139 715 306 180 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2200 2230 2340 Size: 2200 30 110 Fragments arranged by size: 2200 110 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 710 2340 Size: 710 1630 BseYI C'CCAG_C Cuts at: 0 620 2340 Size: 620 1720 BsiEI CG_ry'CG Cuts at: 0 1221 2340 Size: 1221 1119 BsiHKAI G_wGCw'C Cuts at: 0 117 356 964 2114 2213 2340 Size: 117 239 608 1150 99 127 Fragments arranged by size: 1150 608 239 127 117 99 Bsp1286I G_dGCh'C Cuts at: 0 117 152 270 356 500 605 964 1075 Size: 117 35 118 86 144 105 359 111 Cuts at: 1075 1659 1809 1923 2114 2129 2213 2340 Size: 584 150 114 191 15 84 127 Fragments arranged by size: 584 359 191 150 144 127 118 117 114 111 105 86 84 35 15 BspEI T'CCGG_A Cuts at: 0 180 1319 1625 2340 Size: 180 1139 306 715 Fragments arranged by size: 1139 715 306 180 BspHI T'CATG_A Cuts at: 0 378 693 2340 Size: 378 315 1647 Fragments arranged by size: 1647 378 315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 671 1783 2234 2340 Size: 671 1112 451 106 Fragments arranged by size: 1112 671 451 106 BsrBI CCG'CTC Cuts at: 0 1112 2270 2340 Size: 1112 1158 70 Fragments arranged by size: 1158 1112 70 BsrFI r'CCGG_y Cuts at: 0 881 1216 1220 2340 Size: 881 335 4 1120 Fragments arranged by size: 1120 881 335 4 BstXI CCAn_nnnn'nTGG Cuts at: 0 160 364 397 526 676 709 2136 2340 Size: 160 204 33 129 150 33 1427 204 Fragments arranged by size: 1427 204 204 160 150 129 33 33 BstYI r'GATC_y Cuts at: 0 1418 1628 2075 2340 Size: 1418 210 447 265 Fragments arranged by size: 1418 447 265 210 Bsu36I CC'TnA_GG Cuts at: 0 1660 2340 Size: 1660 680 BtgI C'CryG_G Cuts at: 0 270 444 504 522 543 642 675 834 Size: 270 174 60 18 21 99 33 159 Cuts at: 834 915 942 1066 1559 1793 2340 Size: 81 27 124 493 234 547 Fragments arranged by size: 547 493 270 234 174 159 124 99 81 60 33 27 21 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1482 1731 2340 Size: 1482 249 609 Fragments arranged by size: 1482 609 249 ClaI AT'CG_AT Cuts at: 0 2288 2340 Size: 2288 52 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 267 302 2340 Size: 267 35 2038 Fragments arranged by size: 2038 267 35 DraIII CAC_nnn'GTG Cuts at: 0 147 2340 Size: 147 2193 EaeI y'GGCC_r Cuts at: 0 1218 1556 1562 2340 Size: 1218 338 6 778 Fragments arranged by size: 1218 778 338 6 EagI C'GGCC_G Cuts at: 0 1218 2340 Size: 1218 1122 EarI CTCTTCn'nnn_ Cuts at: 0 1267 1627 1786 1792 1873 2318 2340 Size: 1267 360 159 6 81 445 22 Fragments arranged by size: 1267 445 360 159 81 22 6 EcoICRI GAG'CTC Cuts at: 0 115 354 2112 2211 2340 Size: 115 239 1758 99 129 Fragments arranged by size: 1758 239 129 115 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1083 1210 1430 2179 2316 2339 2340 Size: 1083 127 220 749 137 23 1 Fragments arranged by size: 1083 749 220 137 127 23 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 1297 2340 Size: 1297 1043 EcoO109I rG'GnC_Cy Cuts at: 0 386 530 683 761 1656 1674 1721 2340 Size: 386 144 153 78 895 18 47 619 Fragments arranged by size: 895 619 386 153 144 78 47 18 EcoRV GAT'ATC Cuts at: 0 779 1155 2340 Size: 779 376 1185 Fragments arranged by size: 1185 779 376 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1305 2340 Size: 1305 1035 HaeII r_GCGC'y Cuts at: 0 311 1836 1931 2032 2340 Size: 311 1525 95 101 308 Fragments arranged by size: 1525 311 308 101 95 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 27 460 492 1879 1911 2340 Size: 27 433 32 1387 32 429 Fragments arranged by size: 1387 433 429 32 32 27 KasI G'GCGC_C Cuts at: 0 307 1927 2340 Size: 307 1620 413 Fragments arranged by size: 1620 413 307 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 381 630 2340 Size: 381 249 1710 Fragments arranged by size: 1710 381 249 MscI TGG'CCA Cuts at: 0 1558 2340 Size: 1558 782 MslI CAynn'nnrTG Cuts at: 0 158 380 506 524 674 707 806 821 Size: 158 222 126 18 150 33 99 15 Cuts at: 821 1126 1384 1390 1471 1564 1765 1906 2340 Size: 305 258 6 81 93 201 141 434 Fragments arranged by size: 434 305 258 222 201 158 150 141 126 99 93 81 33 18 15 6 MspA1I CmG'CkG Cuts at: 0 1230 1795 2232 2340 Size: 1230 565 437 108 Fragments arranged by size: 1230 565 437 108 NaeI GCC'GGC Cuts at: 0 1222 2340 Size: 1222 1118 NarI GG'CG_CC Cuts at: 0 308 1928 2340 Size: 308 1620 412 Fragments arranged by size: 1620 412 308 NcoI C'CATG_G Cuts at: 0 270 444 522 543 675 834 942 1559 Size: 270 174 78 21 132 159 108 617 Cuts at: 1559 2340 Size: 781 Fragments arranged by size: 781 617 270 174 159 132 108 78 21 NgoMIV G'CCGG_C Cuts at: 0 1220 2340 Size: 1220 1120 PasI CC'CwG_GG Cuts at: 0 1503 1725 1923 2340 Size: 1503 222 198 417 Fragments arranged by size: 1503 417 222 198 PflMI CCAn_nnn'nTGG Cuts at: 0 1338 2340 Size: 1338 1002 PfoI T'CCnGG_A Cuts at: 0 991 1307 1706 2340 Size: 991 316 399 634 Fragments arranged by size: 991 634 399 316 PmlI CAC'GTG Cuts at: 0 1117 2340 Size: 1117 1223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2200 2232 2340 Size: 2200 32 108 Fragments arranged by size: 2200 108 32 PpuMI rG'GwC_Cy Cuts at: 0 386 530 683 761 1674 1721 2340 Size: 386 144 153 78 913 47 619 Fragments arranged by size: 913 619 386 153 144 78 47 PshAI GACnn'nnGTC Cuts at: 0 1702 2340 Size: 1702 638 PspOMI G'GGCC_C Cuts at: 0 1655 2340 Size: 1655 685 PstI C_TGCA'G Cuts at: 0 2124 2231 2340 Size: 2124 107 109 Fragments arranged by size: 2124 109 107 PvuII CAG'CTG Cuts at: 0 2232 2340 Size: 2232 108 SacI G_AGCT'C Cuts at: 0 117 356 2114 2213 2340 Size: 117 239 1758 99 127 Fragments arranged by size: 1758 239 127 117 99 SacII CC_GC'GG Cuts at: 0 1796 2340 Size: 1796 544 SanDI GG'GwC_CC Cuts at: 0 386 1721 2340 Size: 386 1335 619 Fragments arranged by size: 1335 619 386 ScaI AGT'ACT Cuts at: 0 1975 2340 Size: 1975 365 SexAI A'CCwGG_T Cuts at: 0 1991 2340 Size: 1991 349 SfcI C'TryA_G Cuts at: 0 558 2120 2227 2340 Size: 558 1562 107 113 Fragments arranged by size: 1562 558 113 107 SfoI GGC'GCC Cuts at: 0 309 1929 2340 Size: 309 1620 411 Fragments arranged by size: 1620 411 309 SmlI C'TyrA_G Cuts at: 0 302 1489 1633 2044 2340 Size: 302 1187 144 411 296 Fragments arranged by size: 1187 411 302 296 144 StuI AGG'CCT Cuts at: 0 2042 2340 Size: 2042 298 StyI C'CwwG_G Cuts at: 0 270 444 522 543 675 804 834 942 Size: 270 174 78 21 132 129 30 108 Cuts at: 942 1337 1445 1559 2340 Size: 395 108 114 781 Fragments arranged by size: 781 395 270 174 132 129 114 108 108 78 30 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1079 1082 1138 2340 Size: 1079 3 56 1202 Fragments arranged by size: 1202 1079 56 3 TatI w'GTAC_w Cuts at: 0 1973 2340 Size: 1973 367 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 601 633 2340 Size: 601 32 1707 Fragments arranged by size: 1707 601 32 Tth111I GACn'n_nGTC Cuts at: 0 952 1092 2340 Size: 952 140 1248 Fragments arranged by size: 1248 952 140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 487 1566 2340 Size: 487 1079 774 Fragments arranged by size: 1079 774 487 XmnI GAAnn'nnTTC Cuts at: 0 1846 2340 Size: 1846 494 Enzymes that do cut: AcuI AfeI AflIII AleI AloI AlwNI ApaI ApoI BamHI BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtgZI ClaI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI ScaI SexAI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI BsaBI BsgI BsiWI BsmI BsmBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI DraI DrdI EciI EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI ZraI