Restriction map for
pF1KB5798
(Linear) (Six-base) MAPSORT of: KIBB5798.seq Check: 2324 from: 1 to: 2343
KIBB5798.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1392 2343
Size: 1392 951
Acc65I G'GTAC_C
Cuts at: 0 973 2343
Size: 973 1370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 892 1156 1309 1385 1393 1870 2250
Size: 373 519 264 153 76 8 477 380
Cuts at: 2250 2343
Size: 93
Fragments arranged by size:
519 477 380 373 264 153 93 76
8
AfeI AGC'GCT
Cuts at: 0 1370 2343
Size: 1370 973
AflIII A'CryG_T
Cuts at: 0 2278 2343
Size: 2278 65
AhdI GACnn_n'nnGTC
Cuts at: 0 2315 2343
Size: 2315 28
AleI CACnn'nnGTG
Cuts at: 0 94 2343
Size: 94 2249
AlwNI CAG_nnn'CTG
Cuts at: 0 219 240 645 1015 1113 1624 2343
Size: 219 21 405 370 98 511 719
Fragments arranged by size:
719 511 405 370 219 98 21
ApaI G_GGCC'C
Cuts at: 0 2252 2343
Size: 2252 91
ApoI r'AATT_y
Cuts at: 0 773 1265 2343
Size: 773 492 1078
Fragments arranged by size:
1078 773 492
AvaI C'yCGr_G
Cuts at: 0 370 439 660 702 830 1003 1089 2343
Size: 370 69 221 42 128 173 86 1254
Fragments arranged by size:
1254 370 221 173 128 86 69 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 965 998 1487 1520 1899 1932 2343
Size: 965 33 489 33 379 33 411
Fragments arranged by size:
965 489 411 379 33 33 33
BamHI G'GATC_C
Cuts at: 0 116 1994 2343
Size: 116 1878 349
Fragments arranged by size:
1878 349 116
BanI G'GyrC_C
Cuts at: 0 48 655 756 973 1019 1061 2343
Size: 48 607 101 217 46 42 1282
Fragments arranged by size:
1282 607 217 101 48 46 42
BanII G_rGCy'C
Cuts at: 0 257 538 1090 1178 1933 2252 2343
Size: 257 281 552 88 755 319 91
Fragments arranged by size:
755 552 319 281 257 91 88
BbeI G_GCGC'C
Cuts at: 0 659 760 2343
Size: 659 101 1583
Fragments arranged by size:
1583 659 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 361 2343
Size: 361 1982
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 666 700 2106 2140 2343
Size: 666 34 1406 34 203
Fragments arranged by size:
1406 666 203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1515 2343
Size: 1515 828
Bme1580I G_kGCm'C
Cuts at: 0 53 1022 2252 2343
Size: 53 969 1230 91
Fragments arranged by size:
1230 969 91 53
BmgBI CAC'GTC
Cuts at: 0 1458 2343
Size: 1458 885
BmrI ACTGGGnnnn_n'
Cuts at: 0 958 1971 2073 2343
Size: 958 1013 102 270
Fragments arranged by size:
1013 958 270 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 510 2343
Size: 510 1833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 96 344 1300 1417 1438 1471 1567
Size: 58 38 248 956 117 21 33 96
Cuts at: 1567 2343
Size: 776
Fragments arranged by size:
956 776 248 117 96 58 38 33
21
Bpu10I CC'TnA_GC
Cuts at: 0 472 1099 1778 2343
Size: 472 627 679 565
Fragments arranged by size:
679 627 565 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 1634 2343
Size: 748 886 709
Fragments arranged by size:
886 748 709
BsaI GGTCTCn'nnnn_
Cuts at: 0 953 2343
Size: 953 1390
BsaAI yAC'GTr
Cuts at: 0 2281 2343
Size: 2281 62
BsaHI Gr'CG_yC
Cuts at: 0 656 757 2101 2343
Size: 656 101 1344 242
Fragments arranged by size:
1344 656 242 101
BsaWI w'CCGG_w
Cuts at: 0 846 907 2343
Size: 846 61 1436
Fragments arranged by size:
1436 846 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 607 637 950 980 1390 1420 1549 1579
Size: 607 30 313 30 410 30 129 30
Cuts at: 1579 2343
Size: 764
Fragments arranged by size:
764 607 410 313 129 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 265 1252 1327 1348 1637 1666 2294
Size: 55 210 987 75 21 289 29 628
Cuts at: 2294 2343
Size: 49
Fragments arranged by size:
987 628 289 210 75 55 49 29
21
BseYI C'CCAG_C
Cuts at: 0 1106 2343
Size: 1106 1237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 220 450 696 1405 2343
Size: 118 102 230 246 709 938
Fragments arranged by size:
938 709 246 230 118 102
BsiEI CG_ry'CG
Cuts at: 0 400 2343
Size: 400 1943
BsiHKAI G_wGCw'C
Cuts at: 0 110 257 1211 1711 2343
Size: 110 147 954 500 632
Fragments arranged by size:
954 632 500 147 110
BsiWI C'GTAC_G
Cuts at: 0 401 2343
Size: 401 1942
Bsp1286I G_dGCh'C
Cuts at: 0 53 110 257 538 1022 1090 1178 1211
Size: 53 57 147 281 484 68 88 33
Cuts at: 1211 1711 1933 2252 2343
Size: 500 222 319 91
Fragments arranged by size:
500 484 319 281 222 147 91 88
68 57 53 33
BspEI T'CCGG_A
Cuts at: 0 846 2343
Size: 846 1497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 704 1131 1392 2343
Size: 204 500 427 261 951
Fragments arranged by size:
951 500 427 261 204
BsrBI CCG'CTC
Cuts at: 0 1043 2343
Size: 1043 1300
BsrFI r'CCGG_y
Cuts at: 0 147 2343
Size: 147 2196
BstAPI GCAn_nnn'nTGC
Cuts at: 0 219 1383 2343
Size: 219 1164 960
Fragments arranged by size:
1164 960 219
BstBI TT'CG_AA
Cuts at: 0 686 2343
Size: 686 1657
BstEII G'GTnAC_C
Cuts at: 0 171 732 903 2343
Size: 171 561 171 1440
Fragments arranged by size:
1440 561 171 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 168 327 1471 2151 2343
Size: 168 159 1144 680 192
Fragments arranged by size:
1144 680 192 168 159
BstYI r'GATC_y
Cuts at: 0 116 131 1667 1994 2209 2343
Size: 116 15 1536 327 215 134
Fragments arranged by size:
1536 327 215 134 116 15
Bsu36I CC'TnA_GG
Cuts at: 0 1032 2343
Size: 1032 1311
BtgI C'CryG_G
Cuts at: 0 626 2343
Size: 626 1717
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1500 2343
Size: 1500 843
BtsI GCAGTG_nn'
Cuts at: 0 1349 2343
Size: 1349 994
DraIII CAC_nnn'GTG
Cuts at: 0 1224 2343
Size: 1224 1119
EaeI y'GGCC_r
Cuts at: 0 158 245 397 1950 2343
Size: 158 87 152 1553 393
Fragments arranged by size:
1553 393 158 152 87
EagI C'GGCC_G
Cuts at: 0 397 2343
Size: 397 1946
EarI CTCTTCn'nnn_
Cuts at: 0 182 1078 1159 1699 1846 2343
Size: 182 896 81 540 147 497
Fragments arranged by size:
896 540 497 182 147 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 425 2343
Size: 425 1918
EcoICRI GAG'CTC
Cuts at: 0 255 2343
Size: 255 2088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 96 344 373 892 1156 1300 1309
Size: 58 38 248 29 519 264 144 9
Cuts at: 1309 1385 1393 1417 1438 1471 1567 1870 2250
Size: 76 8 24 21 33 96 303 380
Cuts at: 2250 2343
Size: 93
Fragments arranged by size:
519 380 303 264 248 144 96 93
76 58 38 33 29 24 21 9
8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1138 2343
Size: 1138 1205
EcoO109I rG'GnC_Cy
Cuts at: 0 216 501 680 880 2249 2335 2343
Size: 216 285 179 200 1369 86 8
Fragments arranged by size:
1369 285 216 200 179 86 8
FspI TGC'GCA
Cuts at: 0 610 2343
Size: 610 1733
HaeII r_GCGC'y
Cuts at: 0 240 580 659 760 1131 1372 1778 2343
Size: 240 340 79 101 371 241 406 565
Fragments arranged by size:
565 406 371 340 241 240 101 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 1306 1338 1549 1581 1632 1664
Size: 147 32 1127 32 211 32 51 32
Cuts at: 1664 1761 1793 2201 2233 2301 2333 2343
Size: 97 32 408 32 68 32 10
Fragments arranged by size:
1127 408 211 147 97 68 51 32
32 32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 969 2343
Size: 969 1374
KasI G'GCGC_C
Cuts at: 0 655 756 2343
Size: 655 101 1587
Fragments arranged by size:
1587 655 101
KpnI G_GTAC'C
Cuts at: 0 977 2343
Size: 977 1366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1695 1956 2157 2343
Size: 1695 261 201 186
Fragments arranged by size:
1695 261 201 186
MscI TGG'CCA
Cuts at: 0 160 2343
Size: 160 2183
MslI CAynn'nnrTG
Cuts at: 0 94 2161 2343
Size: 94 2067 182
Fragments arranged by size:
2067 182 94
MspA1I CmG'CkG
Cuts at: 0 628 802 941 1149 1278 1380 1913 2234
Size: 628 174 139 208 129 102 533 321
Cuts at: 2234 2343
Size: 109
Fragments arranged by size:
628 533 321 208 174 139 129 109
102
NarI GG'CG_CC
Cuts at: 0 656 757 2343
Size: 656 101 1586
Fragments arranged by size:
1586 656 101
NdeI CA'TA_TG
Cuts at: 0 1491 2343
Size: 1491 852
NspI r_CATG'y
Cuts at: 0 1789 2343
Size: 1789 554
PasI CC'CwG_GG
Cuts at: 0 327 2272 2343
Size: 327 1945 71
Fragments arranged by size:
1945 327 71
PflMI CCAn_nnn'nTGG
Cuts at: 0 2056 2326 2343
Size: 2056 270 17
Fragments arranged by size:
2056 270 17
PfoI T'CCnGG_A
Cuts at: 0 112 1415 2343
Size: 112 1303 928
Fragments arranged by size:
1303 928 112
PmlI CAC'GTG
Cuts at: 0 2281 2343
Size: 2281 62
PpuMI rG'GwC_Cy
Cuts at: 0 216 2343
Size: 216 2127
PspOMI G'GGCC_C
Cuts at: 0 2248 2343
Size: 2248 95
PstI C_TGCA'G
Cuts at: 0 86 215 574 641 997 1195 1586 2343
Size: 86 129 359 67 356 198 391 757
Fragments arranged by size:
757 391 359 356 198 129 86 67
PvuII CAG'CTG
Cuts at: 0 802 1149 1278 1913 2234 2343
Size: 802 347 129 635 321 109
Fragments arranged by size:
802 635 347 321 129 109
SacI G_AGCT'C
Cuts at: 0 257 2343
Size: 257 2086
SacII CC_GC'GG
Cuts at: 0 629 2343
Size: 629 1714
SapI GCTCTTCn'nnn_
Cuts at: 0 1078 1159 1699 2343
Size: 1078 81 540 644
Fragments arranged by size:
1078 644 540 81
SbfI CC_TGCA'GG
Cuts at: 0 215 574 2343
Size: 215 359 1769
Fragments arranged by size:
1769 359 215
ScaI AGT'ACT
Cuts at: 0 1717 2343
Size: 1717 626
SfcI C'TryA_G
Cuts at: 0 82 211 570 637 743 993 1191 1582
Size: 82 129 359 67 106 250 198 391
Cuts at: 1582 2169 2343
Size: 587 174
Fragments arranged by size:
587 391 359 250 198 174 129 106
82 67
SfoI GGC'GCC
Cuts at: 0 657 758 2343
Size: 657 101 1585
Fragments arranged by size:
1585 657 101
SmaI CCC'GGG
Cuts at: 0 662 832 1091 2343
Size: 662 170 259 1252
Fragments arranged by size:
1252 662 259 170
SmlI C'TyrA_G
Cuts at: 0 727 1649 2343
Size: 727 922 694
Fragments arranged by size:
922 727 694
SspI AAT'ATT
Cuts at: 0 280 2343
Size: 280 2063
StuI AGG'CCT
Cuts at: 0 493 1030 2343
Size: 493 537 1313
Fragments arranged by size:
1313 537 493
StyI C'CwwG_G
Cuts at: 0 1212 1553 2343
Size: 1212 341 790
Fragments arranged by size:
1212 790 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 891 2055 2314 2343
Size: 891 1164 259 29
Fragments arranged by size:
1164 891 259 29
TatI w'GTAC_w
Cuts at: 0 1406 1715 2343
Size: 1406 309 628
Fragments arranged by size:
1406 628 309
Tth111I GACn'n_nGTC
Cuts at: 0 2043 2343
Size: 2043 300
XbaI T'CTAG_A
Cuts at: 0 786 2343
Size: 786 1557
XmaI C'CCGG_G
Cuts at: 0 660 830 1089 2343
Size: 660 170 259 1254
Fragments arranged by size:
1254 660 259 170
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspMI
BsrBI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NarI NdeI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SacII SapI SbfI ScaI SfcI SfoI
SmaI SmlI SspI StuI StyI TaqII TatI Tth111I
XbaI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AlfI AloI ApaLI
AscI AseI AsiSI AvrII BbvCI BclI BfrBI BglI
BglII BlpI BmtI BsaBI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BssSI BstZ17I ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI MfeI
MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI TstI XcmI XhoI
XmnI ZraI